Fig. 1

Sequence and positions of targets in OsMATL The black bold letters are the protospacer adjacent motif (PAM) sequences; the red triangle points to the mutation site in the native MTL allele in maize."

Table 1

Primer sequences used in this study"

Fig. 2

Schematic diagram of the CRISPR/Cas9 vector construction A: single-target vector; B: dual-target vector."

Fig. 3

Multiple alignment of MTL homologous genes amino sequences in cereals and A. thaliana The red box highlights the mutation site in the native MTL allele of maize."

Fig. 4

Promoter sequence and major cis-acting elements of OsMATL The blue letters are TATA-box; the gray shadow shows CAAT-box; other elements are underlined whose name remarks under the sequence."

Table 2

Homozygous mutation in coding region and large fragment deletion mutation in promoter region types of T0 generation"

Fig. 5

Tertiary structure prediction and relative expression levels of OsMATL gene in wild type and mutant A: tertiary structure prediction of OsMATL gene in wild type; B: tertiary structure prediction of OsMATL gene in the first exon mutant; C: tertiary structure prediction of OsMATL gene in the third exon mutant; D: tertiary structure prediction of OsMATL gene in the fourth exon mutant; E: relative expression levels of OsMATL gene in promoter region 293 bp deletion mutant. WT is wild type and MT is mutant."

Fig. 6

Seed setting rate and abortion seed rate of wild type and OsMATL mutant ** indicates significant differences at P < 0.01 between OsMATL mutant and wild type."

Fig. 7

Seed setting phenotype and different types of abortion seed phenotypes A, B: the phenotype of seed setting; C, D: the phenotype of abortion seed; the red triangles point to solid grains. Bar = 0.5 cm."