栽培种花生单仁重QTL定位分析

doi:10.3724/SP.J.1006.2023.24190

Abstract

Abstract:

Peanut (Arachis hypogaea L.) is an important oil and cash crop in China, and the single-seed weight is one of the important traits that determine the yield and commodity of peanut. In this study, QTLs mapping for single-seed weight of peanut was performed using RIL population constructed from a cross between large-kernel variety Shanhua 15 (female) and small-kernel variety Zhonghua 12 (male), based on a high-density genetic map. Nine QTLs related to single-seed weight were identified on seven chromosomes (A04, A06, A07, B05, B07, B09, and B10). The LOD values of these QTLs was 3.01-33.97, phenotypic variation contribution rates was 2.61%-34.28%, the additive effect values were -0.03 to 0.15 g, and the physical range of positioning was 0.03-4.32 Mb. qSSWA07.1 was a stable major QTL repeatedly detected in six planting environments, and qSSWA06.1 and qSSWB09.1 were repeatedly detected in four planting environments. The additive effect of qSSWA07.1 and qSSWA06.1 were positive, and the favorable alleles were inherited from Shanhua 15. The additive effect of qSSWB09.1 was negative, and the favorable allele was inherited from Zhonghua 12. The additive effect of qSSWA06.1, qSSWA07.1, and qSSWB09.1 on peanut single-seed weight was analyzed with the genotype of three bin markers (A06: Block3344, A07: Block3373, and B09: Block10032) closely-linked with QTLs. The mean value of single-seed weight of lines with three favorable alleles was the largest, and that of the lines without the favorable alleles was the smallest. The candidate genes in qSSWA06.1, qSSWA07.1, and qSSWB09.1 were analyzed by KEGG enrichment pathway, according to the expression in different tissues and functional annotation of these genes, four candidate genes were predicted, Arahy.9UY90I, Arahy.RX7YKY, Arahy.3ZC2CN, and Arahy.9V2WXE, which may play a significant role in the regulation of peanut seed growth and development. The results provided the reference for the genetic improvement of yield related traits in peanut.

Key words: peanut, single-seed weight, QTLs mapping, candidate genes

LI Xing, YANG Hui, LUO Lu, LI Hua-Dong, ZHANG Kun, ZHANG Xiu-Rong, LI Yu-Ying, YU Hai-Yang, WANG Tian-Yu, LIU Jia-Qi, WANG Yao, LIU Feng-Zhen, WAN Yong-Shan. QTLs mapping for single-seed weight of cultivated peanut[J].Acta Agronomica Sinica, 2023, 49(8): 2160-2170.

Figures/Tables 8

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种植环境 Planting environment	亲本Parent		RIL群体 RIL population
种植环境 Planting environment	山花15号 Shanhua 15	中花12号 Zhonghua 12	最大值 Max.	最小值 Min.	平均值 Mean	标准差 SD	变异系数 CV (%)	偏度 Skewness	峰度 Kurtosis
2014	1.39	0.71^**	1.63	0.41	0.93	0.25	26.88	0.291	-0.413
2015	1.21	0.58^**	1.33	0.22	0.74	0.18	24.32	0.283	0.286
2016	1.08	0.42^**	1.24	0.24	0.64	0.18	28.13	0.414	0.217
2017	1.14	0.75^**	1.52	0.59	0.94	0.19	20.21	0.354	-0.209
2020	1.18	0.76^**	1.57	0.48	0.97	0.21	21.68	0.116	-0.543
2021	1.16	0.76^**	1.21	0.33	0.76	0.17	22.37	0.027	-0.437

QTL	种植环境 Planting environment	染色体 Chr.	标记区间 Marker interval	物理区间长度 Physical interval length (Mb)	阈值 LOD	贡献率 PVE (%)	加性效应 ADD (g)
qSSWA04.1	2015	A04	Block1622-Block1625	2.05	3.01	3.41	-0.03
qSSWA06.1	2021	A06	Block3360-Block3309	4.32	6.82	5.83	0.05
	2020				8.30	7.61	0.05
	2017				10.24	9.40	0.06
	2014				8.11	7.39	0.07
qSSWA07.1	2021	A07	Block3421-Block3366	1.39	31.02	32.30	0.10
	2020				33.62	34.06	0.11
	2017				33.97	34.28	0.12
	2016				13.75	16.36	0.07
	2015				18.57	21.34	0.08
	2014				30.17	31.34	0.15
qSSWB05.1	2020	B05	Block7608-Block7556	0.84	3.12	2.92	0.04
qSSWB05.2	2017	B05	Block7651-Block7587	3.01	4.34	3.33	0.03
qSSWB07.1	2014	B07	Block8272-Block8264	0.40	3.80	4.12	0.05
qSSWB07.2	2020	B07	Block8284-Block8285	0.03	3.08	2.61	0.04
qSSWB09.1	2021	B09	Block10054-Block10004	1.45	5.27	4.21	-0.04
	2020				5.79	4.48	-0.04
	2017				7.12	6.38	-0.04
	2014				6.30	5.39	-0.06
qSSWB10.1	2015	B10	Block10084-Block10083	0.58	3.16	3.36	0.03

基因 ID Gene ID	染色体 Chr.	物理区间 Physical interval (bp)	基因家族 Gene family	功能注释 Functional description
Arahy.9UY90I	A07	447423-452848	legfed_v1_0.L_MWNP2J	烯醇酶 Enolase
Arahy.KE1FF5	A07	955911-958635	legfed_v1_0.L_KG5CQL	半胱氨酸合酶/半胱氨酸β合酶 Cysteine synthase/cystathionine β-synthase
Arahy.K4CMZR	A07	1001166-1002296	legfed_v1_0.L_1XK45N	丝氨酸O-乙酰转移酶 Serine O-acetyltransferase
Arahy.K0DIZR	A07	1218259-1224643	legfed_v1_0.L_0GKWV0	ABC转运蛋白1型 ABC transporter type 1
Arahy.RX7YKY	B09	157391371-157396195	legfed_v1_0.L_BH5DLF	甜菜碱同型半胱氨酸甲基转移酶 Betaine-homocysteine methyltransferase
Arahy.E9249P	B09	157550706-157553539	legfed_v1_0.L_0C7SKC	类RmlC-like cupins蛋白 Proteins similar to the RmlC-like cupins
Arahy.IQP7TJ	B09	157714814-157717184	legfed_v1_0.L_FMT5KJ	糖苷水解酶 Glycoside hydrolase
Arahy.QJT344	A06	110434786-110446942	legfed_v1_0.L_9WXLVF	泛素特异性蛋白酶 Ubiquitin specific proteases
Arahy.3ZC2CN	A06	110489370-110491090	legfed_v1_0.L_63KDF3	LSM蛋白质 LSM proteins
Arahy.S90AI0	A06	112231732-112241188	legfed_v1_0.L_J3GGX7	核转录和剪接因子 Nuclear transcription and splicing factors
Arahy.57RIKC	A06	110715861-110721136	legfed_v1_0.L_F86644	磷酸吡哆醛依赖性转移酶 Pyridoxal phosphate-dependent transferase
Arahy.9V2WXE	A06	111734794-111743008	legfed_v1_0.L_RNPQZ0	真核翻译起始因子 Eukaryotic translation initiation factor

QTLs mapping for single-seed weight of cultivated peanut

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