烟草转录因子NtNAC080在非生物胁迫下的表达分析及功能鉴定

doi:10.3724/SP.J.1006.2023.24193

Abstract

Abstract:

NAC proteins, which constitute one of the largest plant-specific transcription factor families, are widely involved in the regulation of plant development, senescence and stress responses. To explore the function of NtNAC080 in abiotic stress response, qRT-PCR was used to analyze the relative expression pattern of NtNAC080 genes under different abiotic stress treatments. Results showed that the relative expression level of NtNAC080 genes was induced by drought, salt, ABA, MeJA, and SA treatments. The NtNAC080 mutant and WT (K326) plants were used to analyze the phenotypes under salt and drought stresses. Results showed that NtNAC080 knockout tobacco lines had increased tolerance to salt and drought stresses. Under salt and drought stresses treatments, the antioxidant enzymes (SOD, POD, and CAT) activities, soluble protein, and proline contents of these two knockout lines plants were significantly higher than those of WT, while MDA content was significantly lower than that of WT. In contrast, NtNAC080-overexpressing Arabidopsis plants had more sensitive to salt and drought stresses. Furthermore, NtNAC080 mutation in tobacco resulted in the up-regulated expression of abiotic stress-related genes (NtDREB1A, NtKAT2, and NtNHX1) after salt and drought treatments. These results indicated that NtNAC080 could be play a negative role in response to salt and drought stresses by regulating the activity of antioxidant enzymes and the relative expression level of stress-related genes.

Key words: tobacco, NtNAC080, ROS, the relative expression analysis, abiotic stresses

WEN Li-Chao, XIONG Tao, DENG Zhi-Chao, LIU Tao, GUO Cun, LI Wei, GUO Yong-Feng. Expression and functional characterization of NtNAC080 transcription factor gene from Nicotiana tabacumin under abiotic stress[J].Acta Agronomica Sinica, 2023, 49(8): 2171-2182.

Figures/Tables 7

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基因名称 Gene name	基因编号 Accession number	正向引物序列 Forward primer (5°-3°)	反向引物序列 Reverse primer (5°-3°)
NtActin	XM_016618658	ACCTCTATGGCAACATTGTGCTCAG	CTGGGAGCCAAAGCGGTGATT
NtNAC080	XM_016596133	TTCACGACTATTTGATGACAATGCTA	TATTGGTCATTGTGTTTTGGTTGTT
NtDREB1A	XM_016627803	TAACCCCAAGAAGCGAGCAG	TAGCCGCCATTTCTGCAGAA
NtKAT2	XM_016602939	CCGCGCAAATCCAGAAGATG	TGGCCCTCCATTTGTTTGGT
NtERF5	XM_016584910	TGGATGAAGAGCCAAGCCAT	GAATCTCCGCCGCGAATTTC
NtNHX1	XM_016578291	CCCCCACAGAGGCAGTTAAG	GCGCCAGTATCTATGCACCT
NtSOS1	XM_016611193	TCGCTTTGCTTGTTCTTGGC	ACCGCCAACAGAAGATCAGG
AtActin	NM_001338359	TGTGCCAATCTACGAGGGTTT	TTTCCCGCTCTGCTGTTGT
AtDREB1A	NM_118680	TTTCAAACCGCTGAGATGGC	AAGCCGAGTCAGCGAAATTG
AtKAT2	NM_001341273	TTCGCCTTTGATGTCTGCTC	TCTCAAGCCTTGCAAACAGC
AtERF5	NM_124094	CGCAGCTGAAAACACCAAAC	AATTTCCCCCACGGTCTTTG
AtNHX1	NM_122597	GGTGCCATATTTGCTGCAAC	ATCGCGTTGAAGACCACAAC
AtSOS1	NM_126259	ACCAATGAAACTGCGTGGTG	ATGCAGCGAGTGATTGTTGC

Expression and functional characterization of NtNAC080 transcription factor gene from Nicotiana tabacumin under abiotic stress

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