Table 1

Primers used for qRT-PCR"

Table 2

Prediction of physicochemical properties of AhGA3ox family proteins"

Fig. 1

Chromosomal distribution GA3ox gene family in peanut genome The black bars represent the chromosomes. GA3ox genes are marked to the right of the chromosomes. The scale bar on the left indicates the length of the chromosomes."

Fig. 2

Phylogenetic and structural analysis of AhGA3ox gene family and encoded proteins in peanut (A) phylogenetic tree is constructed with amino acid sequences of AhGA3ox; (B) the structure of AhGA3ox genes. Green rectangles, yellow rectangles, and black line represent UTR, CDS, and introns; (C) the structure of AhGA3ox protein. Blue and purple rectangles represent DIOX_N and 2OG-FeII_Oxy domains."

Fig. 3

Phylogenetic analysis of GA3ox family The light blue background was tetraploid wild peanut (Arachis moticolar), the green area was cultivated soybean (Glycine max), the blue area was rice (Oryza sativa), the light gray area was Arabidopsis (Arabidopsis thaliana), the yellow area was A genomic diploid wild peanut (Arachis duranensis), the brown area was wild peanut with B genome diploid (Arachis ipaensis), the purple area was wild soybean (Glycine soja), the red area was tetraploid cultivated peanut (Arachis hypogaea L.). Phylogenetic relationships among 71 GA3ox proteins in peanut, arabidopsis, rice, soybean and wild soybean. GA3ox proteins were divided into 4 classes. The phylogenetic tree is built on the basis of the complete amino acid sequences of GA3ox proteins by MEGA-X with maximum likelihood method. Bootstrap=1000."

Fig. 4

Distribution of conserved motifs in GA3ox proteins Phantom analysis is performed by MEME program. The height of different amino acid represents repeatability. The scale bar at the bottom indicates the length of the motif protein sequence. On the left are the names of the protein series. The different colors on the right correspond to the positions of different conserved motifs on the series, respectively."

Fig. 5

Prediction of cis-acting elements in the promoters of AhGA3ox gene family Different colored boxes represent different cis-elements, and the scale at the bottom represents the length of the promoter sequence."

Table 3

Comparison of agronomic traits of two peanut lines"

Fig. 6

Relative expression pattern of AhGA3ox genes family in different plant tissues of P92 and P92-1 Different lowercase letters means significant difference at the 0.05 probability level in peanut tissue."

Fig. 7

Relative expression pattern of AhGA3ox gene family in the shell of two peanut lines with different pod size S1-S5 represents the 10th, 17th, 24th, 31st, and 38th day after the gynophore enters the soil, respectively. *, ** mean significant difference at the 0.05 and 0.01 probability levels, respectively."