青稞分蘖角度的QTL定位

doi:10.3724/SP.J.1006.2025.31086

Abstract

Abstract:

Tiller angle (TA) is a crucial component of qingke (hulless barley) architecture, significantly influencing lodging resistance and grain yield. To investigate the genetic basis of TA, we constructed a high-density genetic linkage map using reduced- representation genome sequencing on recombinant inbred lines (RILs) developed from two parental lines: ‘Dazhangzi’ (characterized by a loose plant architecture) and ‘Kunlun 10’ (characterized by a compact plant architecture). Quantitative trait locus (QTL) mapping was performed based on phenotypic data collected from multiple environments. Additionally, residual hybrid lines (RHLs) derived from the RILs were used to expand the population and fine-map the major QTL, qTA7H-1. A total of nine QTLs associated with TA were identified across seven chromosomes in qingke, with phenotypic variation explained (PVE) ranging from 6.41% to 33.57%. Two QTLs, qTA3H-1 and qTA7H-1, were consistently detected across various environmental conditions, showing average additive effects of 5.42° (increasing TA) and -3.87° (decreasing TA), respectively. Four RHLs were selected within the initial localization interval of qTA7H-1, and F_8:9 near-isogenic lines (NILs) were developed through self-pollination. To further refine the mapping, fourteen pairs of molecular markers were designed and densely placed within the QTL’s confidence interval, targeting the extreme single lines of the RHLs. Ultimately, qTA7H-1 was fine-mapped to a 9.54 Mb physical interval between markers PC08 (32,252,397) and PA10 (41,790,765) using five types of recombinant individuals. Taken together, this study elucidates the genetic factors controlling TA, providing a foundation for genetic improvement and molecular breeding of qingke with optimized architecture.

Key words: qingke, tiller angle, QTL mapping, major genetic locus

YANG Jing-Fa, YU Xin-Lian, YAO You-Hua, YAO Xiao-Hua, WANG Lei, WU Kun-Lun, LI Xin. QTL mapping of tiller angle in qingke (Hordeum vulgare L.)[J].Acta Agronomica Sinica, 2025, 51(1): 260-272.

Figures/Tables 11

Fig. 1

Table 1

Table 2

Fig. 2

Table 3

Fig. 3

Fig. 4

Table 4

Fig. 5

Fig. 6

Table 5

Functional annotation of candidate genes within qTA7H-1"

基因名称Gene ID	基因注释功能Function
HORVU7Hr1G022220	Cinnamoyl-CoA reductase
HORVU7Hr1G022230	SCP-like extracellular protein
HORVU7Hr1G022250	Nucleoside-triphosphatase
HORVU7Hr1G022270	Phox domain-containing protein
HORVU7Hr1G022310	No apical meristem protein
HORVU7Hr1G022340	F-box family protein
HORVU7Hr1G022410	RNA recognition motif containing protein
HORVU7Hr1G022430	Photosystem II P680 chlorophyll A apoprotein
HORVU7Hr1G022440	OsLonP2: putative lon protease homologue
HORVU7Hr1G022480	Cytochrome P450
HORVU7Hr1G022500	6-phosphofructokinase
HORVU7Hr1G022510	Rad21/Rec8 like protein
HORVU7Hr1G022550	Bacterial transferase hexapeptide domain containing protein
HORVU7Hr1G022560	Dual specificity protein phosphatase
HORVU7Hr1G022570	Profilin domain containing protein
HORVU7Hr1G022580	3-ketoacyl-CoA synthase
HORVU7Hr1G022680	Nucleoside-triphosphatase
HORVU7Hr1G022700	DUF803 domain containing
HORVU7Hr1G022720	3-ketoacyl-CoA synthase
HORVU7Hr1G022770	SnRK1-interacting protein 1
HORVU7Hr1G022780	3-ketoacyl-CoA synthase
HORVU7Hr1G022810	Expressed protein
HORVU7Hr1G022820	Ulp1 protease family, protein
HORVU7Hr1G022910	Transporter family protein
HORVU7Hr1G022940	Expressed protein
HORVU7Hr1G022970	Peptidase, T1 family
HORVU7Hr1G022980	ATEXO70G1
HORVU7Hr1G023000	Nodulin
HORVU7Hr1G023010	Integral membrane protein DUF6 containing protein
HORVU7Hr1G023030	Cytokinin-O-glucosyltransferase 2
HORVU7Hr1G023070	Methyltransferase
HORVU7Hr1G023140	2Fe-2S iron-sulfur cluster binding
HORVU7Hr1G023150	WAX2
HORVU7Hr1G023210	GDSL-like lipase/acylhydrolase
HORVU7Hr1G023250	Expressed protein
HORVU7Hr1G023260	Estradiol 17-beta-dehydrogenase 12
HORVU7Hr1G023270	Containing protein
HORVU7Hr1G023280	3-ketoacyl-CoA synthase
HORVU7Hr1G023320	Male sterility protein
HORVU7Hr1G023380	Phytosulfokine receptor precursor
HORVU7Hr1G023410	Hypothetical protein
HORVU7Hr1G023450	Hypothetical protein
HORVU7Hr1G023500	Cysteine synthase, chloroplast/chromoplast precursor
HORVU7Hr1G023530	3-ketoacyl-CoA synthase
HORVU7Hr1G023560	Serine esterase family protein
HORVU7Hr1G023600	Expressed protein
HORVU7Hr1G023610	OsFBL27: F-box domain and LRR containing protein
HORVU7Hr1G023660	RGH1A
HORVU7Hr1G023730	RGH1A
HORVU7Hr1G023760	CGMC_MAPKCMGC_2_ERK.12: CGMC kinases
HORVU7Hr1G023770	RGH1A
HORVU7Hr1G023800	Expressed protein
HORVU7Hr1G023820	RGH1A
HORVU7Hr1G023890	Erythronate-4-phosphate dehydrogenase
HORVU7Hr1G023900	Flavin monooxygenase
HORVU7Hr1G023910	Flavin monooxygenase
HORVU7Hr1G023920	Expressed protein
HORVU7Hr1G023940	OsMADS25: MADS-box family gene
HORVU7Hr1G023970	Sulfotransferase domain containing protein
HORVU7Hr1G023980	IQ calmodulin-binding motif domain protein
HORVU7Hr1G023990	Expressed protein
HORVU7Hr1G024000	OsMADS25-MADS-box family gene
HORVU7Hr1G024010	MYB family transcription factor
HORVU7Hr1G024060	IQ calmodulin-binding domain containing protein
HORVU7Hr1G024190	GDSL-like lipase/acylhydrolase
HORVU7Hr1G024210	Uncharacterized protein ycf45
HORVU7Hr1G024220	GDSL-like lipase/acylhydrolase
HORVU7Hr1G024240	GDSL-like lipase/acylhydrolase
HORVU7Hr1G024250	GDSL-like lipase/acylhydrolase
HORVU7Hr1G024260	Transcription elongation factor 1
HORVU7Hr1G024270	Short-chain dehydrogenase/reductase
HORVU7Hr1G024310	Expressed protein
HORVU7Hr1G024350	AMP-binding enzyme
HORVU7Hr1G024370	Estradiol 17-beta-dehydrogenase 12
HORVU7Hr1G024400	Expressed protein
HORVU7Hr1G024450	Sas10/Utp3 family protein
HORVU7Hr1G024480	Chalcone and stilbene synthases
HORVU7Hr1G024550	Transposon protein, putative, unclassified
HORVU7Hr1G024590	Wax synthase
HORVU7Hr1G024600	DC1 domain-containing protein
HORVU7Hr1G024670	GDSL-like lipase/acylhydrolase
HORVU7Hr1G024690	Galactosyltransferase family protein
HORVU7Hr1G024790	Helix-loop-helix DNA-binding
HORVU7Hr1G024810	rRNA-processing protein FCF
HORVU7Hr1G024890	rRNA-processing protein FCF
HORVU7Hr1G024900	Glyoxalase resistance protein/dioxygenase
HORVU7Hr1G024920	OsFBDUF60: F-box protein
HORVU7Hr1G024930	OsSub18: putative Subtilisin homologue
HORVU7Hr1G024940	OsSub17: putative Subtilisin homologue
HORVU7Hr1G024950	OsSub17: Putative Subtilisin homologue
HORVU7Hr1G024960	ABC transporter, ATP-binding protein
HORVU7Hr1G024980	ABC transporter, ATP-binding protein
HORVU7Hr1G024990	Histone H3
HORVU7Hr1G025000	ABC transporter, ATP-binding protein
HORVU7Hr1G025040	ABC transporter, ATP-binding protein
HORVU7Hr1G025110	ABC transporter, ATP-binding protein
HORVU7Hr1G025130	Thioesterase family protein
HORVU7Hr1G025160	Histone H3
HORVU7Hr1G025200	Histone H3
HORVU7Hr1G025230	ABC transporter, ATP-binding protein
HORVU7Hr1G025240	Cytochrome P450
HORVU7Hr1G025300	Proline-rich cell wall protein-like
HORVU7Hr1G025340	Cytochrome P450
HORVU7Hr1G025390	Starch synthase

Table 5

References 44

[1]	余鑫莲, 李新, 姚晓华, 姚有华, 白羿雄, 安立昆, 吴昆仑. 青稞早抽穗主效QTL cqHD2H-2的遗传定位及候选基因分析. 作物学报, 2022, 48: 2463-2474.
	Yu X L, Li X, Yao X H, Yao Y H, Bai Y X, An L K, Wu K L. Genetic mapping and candidate gene analysis of the major QTL cqHD2H-2 for early heading in barley (Hordeum vulgare L.). Acta Agron Sin, 2022, 48: 2463-2474 (in Chinese with English abstract).
[2]	吴昆仑, 姚晓华, 姚有华, 白羿雄, 迟德钊. 多元化用途背景下青稞品种选育的思考与实践. 西藏农业科技, 2018, 40(增刊1): 1-2.
	Wu K L, Yao X H, Yao Y H, Bai Y X, Chi D Z. Reflections and practice on breeding barley varieties under the background of diversified uses. Tibet J Agric Sci, 2018, 40(S1): 1-2 (in Chinese with English abstract).
[3]	李家洋. 水稻分蘖数目与分蘖角度的分子机理. 中国基础科学, 2008, 10(3): 14-15.
	Li J Y. Molecular regulation mechanism of rice tillering number and tillering angle. China Basic Sci, 2008, 10(3): 14-15 (in Chinese).
[4]	王文广, 王永红. 作物株型与产量研究进展与展望. 中国科学: 生命科学, 2021, 51: 1366-1375.
	Wang W G, Wang Y H. Crop plant architecture and grain yields. Sci Sin Vitae, 2021, 51: 1366-1375 (in Chinese with English abstract).
[5]	李红斌. 小麦分蘖角度调控基因TaTA1-6D的精细定位. 南京农业大学硕士学位论文, 江苏南京, 2021.
	Li H B. Fine Mapping of Wheat Tillering Angle Regulation Gene TaTA1-6D. MS Thesis of Nanjing Agricultural University, Nanjing, Jiangsu, China, 2021 (in Chinese with English abstract).
[6]	刘兆晔, 于经川, 姜鸿明, 辛庆国, 刘克宁, 赵明. 小麦理想株型的探讨. 中国农学通报, 2010, 26(8): 137-141.
	Liu Z Y, Yu J C, Jiang H M, Xin Q G, Liu K N, Zhao M. A discussion on ideal plant type of wheat. Chin Agric Sci Bull, 2010, 26(8): 137-141 (in Chinese with English abstract).
[7]	周毅. 大麦分蘖角度基因的定位研究. 长江大学硕士学位论文, 湖北荆州, 2017.
	Zhou Y. Mapping of Tillering Angle Genes in Barley. MS Thesis of Yangtze University, Jingzhou, Hubei, China, 2017 (in Chinese with English abstract).
[8]	贺记外. 一个控制水稻分蘖角度主效QTL TAC8的精细定位及候选基因分析. 中国农业科学院博士学位论文, 北京, 2016.
	He J W. Fine Mapping and Candidate Gene Analysis of a Major QTL TAC8 for Controlling Tillering Angle in Rice. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2016 (in Chinese with English abstract).
[9]	Zhang N, Yu H, Yu H, Cai Y Y, Huang L Z, Xu C, Xiong G S, Meng X B, Wang J Y, Chen H F, Liu G F, Jing Y H, Yuan Y D, Liang Y, Li S J, Smith S M, Li J Y, Wang Y H. A core regulatory pathway controlling rice tiller angle mediated by the LAZY1- dependent asymmetric distribution of auxin. Plant Cell, 2018, 30: 1461-1475.
[10]	赵德辉. 小麦分蘖角度遗传解析与标记发掘. 西北农林科技大学博士学位论文, 陕西杨凌, 2020.
	Zhao D H. Genetic Analysis and Marker Excavation of Tillering Angle in Wheat. PhD Dissertation of Northwest A & F University, Yangling, Shaanxi, China, 2020 (in Chinese with English abstract).
[11]	Zhong X H, Peng S B, Sanico A L, Liu H X. Quantifying the interactive effect of leaf nitrogen and leaf area on tillering of rice. J Plant Nutr, 2003, 26: 1203-1222.
[12]	于亚辉, 徐正进. 不同栽培条件下水稻分蘖角度动态变化分析. 中国农学通报, 2006, 22(3): 179-181.
	Yu Y H, Xu Z J. Analysis on dynamic change of tiller angle under different cultivation conditions in rice. Chin Agric Sci Bull, 2006, 22(3): 179-181 (in Chinese with English abstract).
[13]	Dong H J, Zhao H, Xie W B, Han Z M, Li G W, Yao W, Bai X F, Hu Y, Guo Z L, Lu K, Yang L, Xing Y Z. A novel tiller angle gene, TAC3 together with TAC1 and D2 largely determine the natural variation of tiller angle in rice cultivars. PLoS Genet, 2016, 12: e1006412.
[14]	Talamé V, Sanguineti M C, Chiapparino E, Bahri H, Ben salem M, Forster B P, Ellis R P, Rhouma S, Zoumarou W, Waugh R, Tuberosa R. Identification of Hordeum spontaneum QTL alleles improving field performance of barley grown under rainfed conditions. Ann Appl Biol, 2004, 144: 309-319.
[15]	Zang J J, Yang X, Moolhuijzen P, Li C D, Bellgard M, Lance R, Apples R. Towards isolation of the barley green revolution gene. In: 12th Australian Barley Technical Symposium, 11-14 Sep. 2005, Hobart, Tasmania.
[16]	周红. 野生大麦重要农艺性状的遗传解析及ceRNA调控网络挖掘. 四川农业大学博士学位论文, 四川雅安, 2020.
	Zhou H. Genetic Analysis of Important Agronomic Characters of Wild Barley and Excavation of ceRNA Regulatory Network. PhD Dissertation of Sichuan Agricultural University, Ya’an, Sichuan, China, 2020 (in Chinese with English abstract).
[17]	Li Z K, Paterson A H, Pinson S R M, Stansel J W. RFLP facilitated analysis of tiller and leaf angles in rice (Oryza sativa L.). Euphytica, 1999, 109: 79-84.
[18]	钱前, 何平, 滕胜, 曾大力, 朱立煌. 水稻分蘖角度的QTLs分析. 遗传学报, 2001, 28: 29-32.
	Qian Q, He P, Teng S, Zeng D L, Zhu L H. QTLs analysis of tiller angle in rice (Oryza sativa L.). Acta Genet Sin, 2001, 28: 29-32 (in Chinese with English abstract).
[19]	Thomson M J, Tai T H, McClung A M, Lai X H, Hinga M E, Lobos K B, Xu Y, Martinez C P, McCouch S R. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107: 479-493.
[20]	余传元, 刘裕强, 江玲, 王春明, 翟虎渠, 万建民. 水稻分蘖角度的QTL定位和主效基因的遗传分析. 遗传学报, 2005, 32: 948-954.
	Yu C Y, Liu Y Q, Jiang L, Wang C M, Zhai H Q, Wan J M. QTLs mapping and genetic analysis of tiller angle in rice (Oryza sativa L.). Acta Genet Sin, 2005, 32: 948-954 (in Chinese with English abstract).
[21]	沈圣泉, 庄杰云, 包劲松, 郑康乐, 夏英武, 舒庆尧. 水稻分蘖最大角度的QTL分析. 农业生物技术学报, 2005, 13: 16-20.
	Shen S Q, Zhuang J Y, Bao J S, Zheng K L, Xia Y W, Shu Q Y. Analysis of QTLs with additive, epistasis and G×E interaction effects of the tillering angle trait in rice. J Agric Biotechnol, 2005, 13: 16-20 (in Chinese with English abstract).
[22]	Li C B, Zhou A L, Sang T. Genetic analysis of rice domestication syndrome with the wild annual species, Oryza nivara. New Phytol, 2006, 170: 185-193.
[23]	Yu B S, Lin Z W, Li H X, Li X J, Li J Y, Wang Y H, Zhang X, Zhu Z F, Zhai W X, Wang X K, Xie D X, Sun C Q. TAC1, a major quantitative trait locus controlling tiller angle in rice. Plant J, 2007, 52: 891-898.
[24]	Wu X R, Tang D, Li M, Wang K J, Cheng Z K. Loose Plant Architecture1, an INDETERMINATE DOMAIN protein involved in shoot gravitropism, regulates plant architecture in rice. Plant Physiol, 2013, 161: 317-329.
[25]	Li P J, Wang Y H, Qian Q, Fu Z M, Wang M, Zeng D L, Li B H, Wang X J, Li J Y. LAZY1 controls rice shoot gravitropism through regulating polar auxin transport. Cell Res, 2007, 17: 402-410.
[26]	Wu Y Z, Zhao S S, Li X R, Zhang B S, Jiang L Y, Tang Y Y, Zhao J, Ma X, Cai H W, Sun C Q, Tan L B. Deletions linked to PROG1 gene participate in plant architecture domestication in Asian and African rice. Nat Commun, 2018, 9: 4157.
[27]	Xie W B, Feng Q, Yu H H, Huang X H, Zhao Q, Xing Y Z, Yu S B, Han B, Zhang Q F. Parent-independent genotyping for constructing an ultrahigh-density linkage map based on population sequencing. Proc Natl Acad Sci USA, 2010, 107: 10578-10583.
[28]	McCouch S R. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11-13.
[29]	Li H. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. arXiv: Genomics, 2013, 1303: 3997v2.
[30]	Choi M S, Woo M O, Koh E B, Lee J, Ham T H, Seo H S, Koh H J. Teosinte Branched 1 modulates tillering in rice plants. Plant Cell Rep, 2012, 31: 57-65.
[31]	康文启, 欧阳由男, 章善庆, 董成琼, 朱练峰, 禹盛苗, 许德海, 金千瑜. 分蘖角度动态型水稻的形态特征及生长特性分析. 中国水稻科学, 2007, 21: 372-378.
	Kang W Q, Ou-Yang Y N, Zhang S Q, Dong C Q, Zhu L F, Yu S M, Xu D H, Jin Q Y. Morphological and ontogenic characterization of rice with dynamic tiller angle. Chin J Rice Sci, 2007, 21: 372-378 (in Chinese with English abstract).
[32]	徐云碧, 申宗坦. 早籼稻品种分蘖角度的遗传分析. 浙江农业学报, 1993, 5(1): 1-5.
	Xu Y B, Shen Z T. Genetic analysis of tiller angles for early season indica rice. Acta Agric Zhejiangensis, 1993, 5(1): 1-5 (in Chinese with English abstract).
[33]	谢元璋, 夏仲炎. 粳稻品种分蘖状的遗传研究. 安徽农业科学, 1994, 22: 319-322.
	Xie Y Z, Xia Z Y. Genetical study on tillering characters of japonica rice varieties. J Anhui Agric Sci, 1994, 22: 319-322 (in Chinese with English abstract)
[34]	陈文帅. 小麦分蘖角度QTL定位. 四川农业大学硕士学位论文, 四川雅安, 2019.
	Chen W S. QTL Mapping of Tillering Angle in Wheat. MS Thesis of Sichuan Agricultural University, Ya’an, Sichuan, China, 2019 (in Chinese with English abstract).
[35]	Dabbert T, Okagaki R J, Cho S, Heinen S E, Boddu J, Muehlbauer G J. The genetics of barley low-tillering mutants: low number of tillers-1 (lnt1). Theor Appl Genet, 2010, 121: 705-715.
[36]	Okagaki R J, Cho S, Kruger W M, Xu W W, Heinen S E, Muehlbauer G J. The barley UNICULM2 gene resides in a centromeric region and may be associated with signaling and stress responses. Funct Integr Genomics, 2013, 13: 33-41.
[37]	欧阳由男, 李春生, 章善庆, 王会民, 朱练峰, 禹盛苗, 金千瑜, 张国平. 光周期和有效积温对水稻分蘖角度动态变化的影响. 应用生态学报, 2009, 20: 1099-1104.
	Ou-Yang Y N, Li C S, Zhang S Q, Wang H M, Zhu L F, Yu S M, Jin Q Y, Zhang G P. Dynamic changes of rice (Oryza sativa L.) tiller angle under effects of photoperiod and effective accumulated temperature. Chin J Appl Ecol, 2009, 20: 1099-1104 (in Chinese with English abstract).
[38]	Marone D, Rodriguez M, Saia S, Papa R, Rau D, Pecorella I, Laidò G, Pecchioni N, Lafferty J, Rapp M, Longin F H, De Vita P. Genome-wide association mapping of prostrate/erect growth habit in winter durum wheat. Int J Mol Sci, 2020, 21: 394.
[39]	Okamura M, Hirose T, Hashida Y, Ohsugi R, Aoki N. Suppression of starch synthesis in rice stems splays tiller angle due to gravitropic insensitivity but does not affect yield. Funct Plant Biol, 2014, 42: 31-41.
[40]	曹鑫, 邓梅, 张正丽, 刘宇娇, 杨希兰, 周红, 刘亚西. 小麦分蘖角度TaTAC1基因同源克隆及表达分析. 植物遗传资源学报, 2017, 18: 125-132.
	Cao X, Deng M, Zhang Z L, Liu Y J, Yang X L, Zhou H, Liu Y X. Molecular characterization and expression analysis of TaTAC1 gene in Triticum aestivum L. J Plant Genet Resour, 2017, 18: 125-132 (in Chinese with English abstract).
[41]	Heang D, Sassa H. An atypical bHLH protein encoded by positive regulator of grain length 2 is involved in controlling grain length and weight of rice through interaction with a typical bHLH protein apg. Breed Sci, 2012, 62: 133-141.
[42]	Jang S, An G, Li H Y. Rice leaf angle and grain size are affected by the OsBUL1 transcriptional activator complex. Plant Physiol, 2017, 173: 688-702.
[43]	Carretero-Paulet L, Galstyan A, Roig-Villanova I, Martínez- García J F, Bilbao-Castro J R, Robertson D L. Genome-wide classification and evolutionary analysis of the bHLH family of transcription factors in Arabidopsis, poplar, rice, moss, and algae. Plant Physiol, 2010, 153: 1398-1412.
[44]	Dong H J, Zhao H, Li S L, Han Z M, Hu G, Liu C, Yang G Y, Wang G W, Xie W B, Xing Y Z. Genome-wide association studies reveal that members of bHLH subfamily 16 share a conserved function in regulating flag leaf angle in rice (Oryza sativa). PLoS Genet, 2018, 14: e1007323.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Li Shaoqing, Li Yangsheng, Wu Fushun, Liao Jianglin, Li Damo. Optimum Fertilization and Its Corresponding Mechanism under Complete Submergence at Booting Stage in Rice[J]. Acta Agronomica Sinica, 2002, 28(01): 115 -120 .
[2]	Wang Lanzhen;Mi Guohua;Chen Fanjun;Zhang Fusuo. Response to Phosphorus Deficiency of Two Winter Wheat Cultivars with Different Yield Components[J]. Acta Agron Sin, 2003, 29(06): 867 -870 .
[3]	YANG Jian-Chang;ZHANG Jian-Hua;WANG Zhi-Qin;ZH0U Qing-Sen. Changes in Contents of Polyamines in the Flag Leaf and Their Relationship with Drought-resistance of Rice Cultivars under Water Deficiency Stress[J]. Acta Agron Sin, 2004, 30(11): 1069 -1075 .
[4]	Yan Mei;Yang Guangsheng;Fu Tingdong;Yan Hongyan. Studies on the Ecotypical Male Sterile-fertile Line of Brassica napus L.Ⅲ. Sensitivity to Temperature of 8-8112AB and Its Inheritance[J]. Acta Agron Sin, 2003, 29(03): 330 -335 .
[5]	Wang Yongsheng;Wang Jing;Duan Jingya;Wang Jinfa;Liu Liangshi. Isolation and Genetic Research of a Dwarf Tiilering Mutant Rice[J]. Acta Agron Sin, 2002, 28(02): 235 -239 .
[6]	WANG Li-Yan;ZHAO Ke-Fu. Some Physiological Response of Zea mays under Salt-stress[J]. Acta Agron Sin, 2005, 31(02): 264 -268 .
[7]	TIAN Meng-Liang;HUNAG Yu-Bi;TAN Gong-Xie;LIU Yong-Jian;RONG Ting-Zhao. Sequence Polymorphism of waxy Genes in Landraces of Waxy Maize from Southwest China[J]. Acta Agron Sin, 2008, 34(05): 729 -736 .
[8]	HU Xi-Yuan;LI Jian-Ping;SONG Xi-Fang. Efficiency of Spatial Statistical Analysis in Superior Genotype Selection of Plant Breeding[J]. Acta Agron Sin, 2008, 34(03): 412 -417 .
[9]	WANG Yan;QIU Li-Ming;XIE Wen-Juan;HUANG Wei;YE Feng;ZHANG Fu-Chun;MA Ji. Cold Tolerance of Transgenic Tobacco Carrying Gene Encoding Insect Antifreeze Protein[J]. Acta Agron Sin, 2008, 34(03): 397 -402 .
[10]	ZHENG Xi;WU Jian-Guo;LOU Xiang-Yang;XU Hai-Ming;SHI Chun-Hai. Mapping and Analysis of QTLs on Maternal and Endosperm Genomes for Histidine and Arginine in Rice (Oryza sativa L.) across Environments[J]. Acta Agron Sin, 2008, 34(03): 369 -375 .

引物名称 Primer name	正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
PA04	TTCCAATGTGGCAGTCATCTATGT	GTGTGAAACTCACACAAGCATAGTCC
PA05	AATAAACACCAACGGCAGGTTATG	ATGGTTGGTGGAACCTTCTCATTA
PA07	AGCATGACTCTCATATCACCATCT	ACGGTGTGGTGTTTTATGGATATT
PA10	TTCACAGAAAGGTGACATGAGGAT	CTTCACTAAACGGGGTAATAAGCG
PA11	CTGCTATTTAGTAGTAGGGCGTGT	TGGCACTACTAGGGAAACACTATG
PA12	TGACTGCTTCCGCTGAGATATTTA	TGAATTCTGGAGTCTTCCCTTTGT
PA14	GGGTCTACCTTATGGGCTACTCTA	AATGCATGATGAAAAGTGGTGTGT
PC01	AAGGAACCATAGCTCTTGTTTTGC	GACTTCACCAACAGAGTCCTAGAG
PC06	CAAAAGGCATAATTCACATAGCCG	GCCAAGGCGAACAAGAACAG
PC07	GCCAACCTGAGCCTAACTTAAATC	ACACTGTACATATTGGCAAGTCCT
PC08	TAGCACTCTAGTCCAATGTCGAAC	AGTTTATCAAGCTTCCCGATCCAT
PC11	TATTCCATTGCTTGATGCTGATCG	AAACTTCGGTAAAGAGACGAGACA
PC12	CTACGGACGAAAACAAACGAGAAA	CCTCACCTAATAAGTTCTGCTGGT
PC13	TGGAGTGTAATTTCTAACTCCGCA	ACTCGTGTTAGTTGTCTAGAGTGG

环境 Environment	亲本 Parents		F₁(°)	RIL群体 RIL lines				遗传力 H²
环境 Environment	达章紫 DZZ (°)	昆仑10号 KL10 (°)	F₁(°)	范围 Range (°)	平均值±SD Mean ± SD (°)	偏度 Skewness	峰度 Kurtosis	遗传力 H²
2022YM	57.17±16.82	30.42±4.26^*	37.47±3.09	15.97-52.35	32.01±7.58	0.56	0.11	0.86
2023MY	40.01±6.31	27.19±2.15^**	39.46±7.38	16.94-49.24	31.92±7.77	0.42	0.05
2023XN	64.48±7.07	32.74±5.54^**	39.91±14.62	24.09-83.77	43.11±11.07	1.08	1.69
2024XN	57.56±10.73	24.94±3.74^**	32.23±3.23	21.78-73.22	40.21±11.29	0.76	0.12

环境 Environment	2022元谋 2022YM	2023西宁 2023XN	2023门源 2023MY	2024西宁 2024XN
2022YM	1.000
2023XN	0.229^*	1.000
2023MY	0.008	0.337^**	1.000
2024XN	0.154^*	0.298^**	0.260^**	1.000

位点 QTL locus	染色体 Chr.	环境 Environment	侧翼标记 Flanking markers	阈值 LOD	表型贡献率 R² (%)	加性效应 Additive effect (°)	遗传区间 Confidence interval (cM)
qTA1H-1	1H	2022YM	c01b155-c01b158	3.77	10.31	3.18	121.00-123.40
qTA1H-2	1H	2022YM	c01b172-c01b176	2.56	6.73	2.53	132.70-133.90
qTA3H-1	3H	2022YM	c03b001-c03b006	2.60	6.97	2.08	0-2.40
		2023XN	c03b001-c03b006	4.73	11.89	4.59	0-2.40
		2024XN	c03b001-c03b008	2.90	10.79	3.76	0-4.50
		2022YM (BSA)	c03b001-c03b006	2.46	11.89	4.18	0-2.50
		2022XN (BSA)	c03b001-c03b004	5.04	25.61	12.51	0-1.50
qTA3H-2	3H	2023XN	c03b016-c03b023	2.55	6.70	3.30	8.80-12.90
qTA3H-3	3H	2024XN	c03b037-c03b039	3.10	11.50	3.84	26.60-29.70
qTA4H-1	4H	2023MY	c04b136-c04b142	2.85	8.09	1.82	119.60-127.00
qTA6H-1	6H	2023MY	c06b121-c06b130	2.65	6.41	3.11	120.90-129.40
qTA7H-1	7H	2022YM	c07b022-c07b033	4.23	12.56	3.00	26.80-43.50
		2023MY	c07b025-c07b033	2.69	7.56	1.73	28.80-43.30
		2022YM (BSA)	c07b026-c07b027	4.66	32.25	6.89	30.50-34.50
qTA7H-2	7H	2024XN	c07b159-c07b165	3.15	11.69	3.87	130.80-133.50
qTA7H-2	7H	2024XN (BSA)	c07b161-c07b162	10.40	33.57	13.42	131.50-133.50

QTL mapping of tiller angle in qingke (Hordeum vulgare L.)

RichHTML432

PDF

Abstract

Cite this article

share this article

Figures/Tables 11

References 44

Related Articles 15

Metrics

Comments

Recommended 10

[1]	YONG Rui, HU Wen-Jing, WU Di, WANG Zun-Jie, LI Dong-Sheng, ZHAO Die, YOU Jun-Chao, XIAO Yong-Gui, WANG Chun-Ping. Identification and validation of quantitative trait loci for grain number per spike showing pleiotropic effect on thousand grain weight in bread wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2025, 51(2): 312-323.
[2]	GUO Shu-Hui, PAN Zhuan-Xia, ZHAO Zhan-Sheng, YANG Liu-Liu, HUANG-FU Zhang-Long, GUO Bao-Sheng, HU Xiao-Li, LU Ya-Dan, DING Xiao, WU Cui-Cui, LAN Gang, LYU Bei-Bei, TAN Feng-Ping, LI Peng-Bo. Genetic analysis of a major fiber length locus on chromosome D11 of upland cotton [J]. Acta Agronomica Sinica, 2025, 51(2): 383-394.
[3]	HAN Li, TANG Sheng-Sheng, LI Jia, HU Hai-Bin, LIU Long-Long, WU Bin. Construction of SNP high-density genetic map and localization of QTL for β-glucan content in oats [J]. Acta Agronomica Sinica, 2024, 50(7): 1710-1718.
[4]	BI Jun-Ge, ZENG Zhan-Kui, LI Qiong, HONG Zhuang-Zhuang, YAN Qun-Xiang, ZHAO Yue, WANG Chun-Ping. QTL mapping and KASP marker development of grain quality-relating traits in two wheat RIL populations [J]. Acta Agronomica Sinica, 2024, 50(7): 1669-1683.
[5]	QIN Na, YE Zhen-Yan, ZHU Can-Can, FU Sen-Jie, DAI Shu-Tao, SONG Ying-Hui, JING Ya, WANG Chun-Yi, LI Jun-Xia. QTL mapping for flavonoid content and seed color in foxtail millet [J]. Acta Agronomica Sinica, 2024, 50(7): 1719-1727.
[6]	ZHENG Xue-Qing, WANG Xing-Rong, ZHANG Yan-Jun, GONG Dian-Ming, QIU Fa-Zhan. Mapping of QTL for ear-related traits and prediction of key candidate genes in maize [J]. Acta Agronomica Sinica, 2024, 50(6): 1435-1450.
[7]	ZHANG Yue, WANG Zhi-Hui, HUAI Dong-Xin, LIU Nian, JIANG Hui-Fang, LIAO Bo-Shou, LEI Yong. Research progress on genetic basis and QTL mapping of oil content in peanut seed [J]. Acta Agronomica Sinica, 2024, 50(3): 529-542.
[8]	HAO Qian-Lin, YANG Ting-Zhi, LYU Xin-Ru, QIN Hui-Min, WANG Ya-Lin, JIA Chen-Fei, XIA Xian-Chun, MA Wu-Jun, XU Deng-An. QTL mapping and GWAS analysis of coleoptile length in bread wheat [J]. Acta Agronomica Sinica, 2024, 50(3): 590-602.
[9]	WU Jia-Jun, TU Ran-Ran, ZHANG Qiu-Li, ZOU Qin-Wen, SUN Zhi-Hao, WANG Hong, HE Guang-Hua. Overexpression of OsPIN2 increases tiller angle by reducing shoot gravitropic response in rice [J]. Acta Agronomica Sinica, 2024, 50(12): 2962-2970.
[10]	LIU Ting-Xuan, GU Yong-Zhe, ZHANG Zhi-Hao, WANG Jun, SUN Jun-Ming, QIU Li-Juan. Mapping soybean protein QTLs based on high-density genetic map [J]. Acta Agronomica Sinica, 2023, 49(6): 1532-1541.
[11]	YANG Jun-Fang, WANG Zhou, QIAO Lin-Yi, WANG Ya, ZHAO Yi-Ting, ZHANG Hong-Bin, SHEN DengGao, WANG HongWei, CAO Yue. QTL mapping of seed size traits based on a high-density genetic map in castor [J]. Acta Agronomica Sinica, 2023, 49(3): 719-730.
[12]	YANG Bin, QIAO Ling, ZHAO Jia-Jia, WU Bang-Bang, WEN Hong-Wei, ZHANG Shu-Wei, ZHENG Xing-Wei, ZHENG Jun. QTL mapping and validation of chlorophyll content of flag leaves in wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2023, 49(3): 744-754.
[13]	YANG Shuo, WU Yang-Chun, LIU Xin-Lei, TANG Xiao-Fei, XUE Yong-Guo, CAO Dan, WANG Wan, LIU Ting-Xuan, QI Hang, LUAN Xiao-Yan, QIU Li-Juan. Fine mapping of qPRO-20-1 related to high protein content in soybean [J]. Acta Agronomica Sinica, 2023, 49(2): 310-320.
[14]	SUN Jian-Qiang, HONG Hui-Long, ZHANG Yong, GU Yong-Zhe, GAO Hua-Wei, ZHOU Ya, CAO Jie, QI Hang, ZHAO Quan, BAO Li-Gao, CHEN Qing-Shan, QIU Li-Juan. Mapping of stable QTL qSW20-1 for 100-seed weight and its effect on yield and quality in soybean [J]. Acta Agronomica Sinica, 2023, 49(10): 2621-2632.
[15]	CAO Hui-Min, YANG Xian-Li, WANG Li-Zhi, LI Ping-Ping, ZHAI Lai-Yuan, JIANG Shu-Kun, ZHENG Tian-Qing, QIU Xian-Jin, XU Jian-Long. QTL identification and favorable allele mining of cold tolerance at seedling stage by reciprocal introgression and recombinant inbred line populations in rice [J]. Acta Agronomica Sinica, 2023, 49(10): 2633-2642.