甜菜HIPPs基因家族鉴定与镉胁迫下的表达分析

doi:10.3724/SP.J.1006.2023.34010

Abstract

Abstract:

Cadmium (Cd) ion balance and detoxification regulation are the cores of exploring cadmium tolerance mechanism in sugar beet, and the basis of heavy metal bioremediation using sugar beet. Heavy metal-associated isoprene plant proteins (HIPPs) are a class of multifunctional metal chaperone proteins, which may play a key role in absorption, transport, and compartmentalization of Cd ions. In the transcriptome expression profile of sugar beet responding to cadmium stress, BvHIPPs were found to be differentially expressed. Based on the above results, the whole BvHIPPs gene family members in beet genome was identified by bioinformatics method, and their physicochemical properties, evolutionary relationships, gene structure, cis-acting elements, chromosome localization, transcription, and expression characteristics under cadmium stress were analyzed. The results showed that there were 23 BvHIPPs family members in the beet genome, all of which contained HMA domains and isoprenylation motif, and 16 BvHIPPs were predicted to be located in the nucleus. According to cis-acting element analysis, beet BvHIPPs can participate in a variety of biological and abiotic stress responses. Transcriptomic analysis showed that all 23 BvHIPPs differentially participated in sugar beet in response to Cd, and qRT-PCR analysis verified furtherly the regulatory characteristics of the BvHIPPs correlated with Cd response. BvHIPPs might play an essential role in the adaptation of sugar beet to cadmium stress. The results suggest that BvHIPPs may play an important role in the process of beet adaptation to cadmium stress, and the results will lay a foundation for the molecular mechanism of beet bioremediation in heavy metal pollution.

Key words: sugar beet, HIPPs, cadmium stress, gene family identification, transcriptional expression characteristics

ZHAO Xiao-Xin, HUANG Shuo-Qi, TAN Wen-Bo, XING Wang, LIU Da-Li. Identification and relative expression profile of HIPPs gene family cadmium stress in sugar beet[J].Acta Agronomica Sinica, 2023, 49(12): 3302-3314.

Figures/Tables 8

Table 1

Table 2

Basic information of BvHIPPs gene family in Beta vulgaris L."

基因 Gene name	氨基酸数 Amino acid number	分子量 Relative molecular mas (kD)	等电点 Isoelectric point	不稳定系数 Instability index	脂肪系数 Aliphatic index	平均亲水性 Hydrophilic coefficient	亚细胞定位 Subcellular localization
BVRB_6g129220	131	14.52	6.76	39.11	72.82	-0.355	未定位Not located
BVRB_7g161540	355	38.77	9.06	32.26	50.45	-1.251	细胞核 Nucleus
BVRB_5g126430	343	38.33	6.00	48.78	51.40	-1.301	细胞核 Nucleus
BVRB_3g050800	340	37.64	4.91	71.30	65.06	-0.926	细胞核 Nucleus
BVRB_2g043150	149	16.82	9.45	43.45	71.81	-0.556	细胞核 Nucleus
BVRB_2g027600	147	16.52	9.28	50.11	66.26	-0.559	细胞核 Nucleus
BVRB_6g135550	155	17.25	9.42	33.95	64.71	-0.561	未定位Not located
BVRB_1g021330	147	16.38	9.39	17.54	65.65	-0.416	线粒体 Mitochondrion
BVRB_9g214040	158	18.07	9.01	40.36	65.82	-0.676	线粒体, 细胞核 Mitochondrion, nucleus
BVRB_5g104360	143	16.47	9.17	34.27	70.77	-0.617	细胞核 Nucleus
BVRB_3g055490	134	14.84	6.28	36.58	72.84	-0.249	未定位Not located
BVRB_4g081750	508	53.07	9.35	47.01	32.62	-1.273	细胞核 Nucleus
BVRB_3g069170	562	55.92	9.85	49.7	34.63	-0.894	细胞核 Nucleus
BVRB_7g164120	417	44.63	8.11	67.82	58.56	-0.823	细胞核 Nucleus
BVRB_1g004050	167	19.32	7.60	52.93	65.93	-1.255	细胞核 Nucleus
BVRB_4g075740	345	37.67	6.19	52.19	45.16	-1.208	细胞核 Nucleus
BVRB_5g122000	137	14.99	9.05	41.58	73.14	-0.381	细胞核 Nucleus
BVRB_8g184430	297	34.30	6.62	56.84	62.59	-1.118	细胞核 Nucleus
BVRB_3g053290	132	14.88	8.85	40.62	73.03	-0.767	细胞核 Nucleus
BVRB_3g053300	127	14.16	8.28	27.07	94.88	-0.181	细胞核 Nucleus
BVRB_6g142690	101	11.21	9.67	48.14	100.99	-0.149	叶绿体, 细胞核 Chloroplast, nucleus
BVRB_3g048110	370	41.00	5.70	76.65	42.16	-1.253	细胞核 Nucleus
BVRB_3g053310	113	12.70	8.37	24.08	81.77	-0.295	叶绿体, 细胞质, 细胞核Chloroplast, cytoplasm, nucleus

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

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基因Gene name	上游引物Forward primer (5°-3°)	下游引物Reverse primer (5°-3°)
BVRB_1g021330	AAGCAACAGAAGGTGACGGT	GGGTGTGCCACCATAGTGTA
BVRB_3g050800	ATAAAAGGCGTGGTGGAGCC	GGGTTCACCCTCATTTGCCG
BVRB_4g075740	AACACGGCGTATCCTAGCAG	TATCAGACGGTGGAACTGCG
BVRB_6g135550	GCAAAAGCCACAGGGAAGAAG	GTCCACCTTCCTGACATGACC
BVRB_3g053310	CTCAGTTGGGCCAGCAAAAG	GTACGGAGGGTTGTAGCCG
BVRB_6g129220	GCCCGATCTGAAGTGTCCAT	CTATGGGCCTGCCATCACAA
BVRB_7g161540	TTGTCCGCAAGTGTGATGGA	CCTTCTTTGCAGGAGCAAGC
BVRB_5g126430	TCCTATTCCGAAACCACCCG	GCATGTGAACCTTGAGCACC
BVRB_2g043150	TGTGGAGCCAAATAGGGTGC	TTTGACATAGCCGGAGGGTG
BVRB_2g027600	ACAGGGCACGTAGATCCAAA	GCCAGCAGGTGCTCTCTTAT
BVRB_9g214040	CGTGTGAAGCATCGAACAGG	ACTCCAGGAGCGTAAGGGT
BVRB_5g104360	GTTCTGGCCTTACCCTAACCC	GAGGGTGCCATGTCTATCACC
BVRB_3g055490	ATCAAAAGGGCAGGCAAAGC	CCGTCGGAAGTGTCGTAGTAA
BVRB_4g081750	TTGCCAGCTTCCCACATGAT	TGCGGATTCATCGGTACTGG
BVRB_3g069170	AAATGGGCCCTATGGGTTCG	TGGAAGTATTCGGGTGCTGC
BVRB_7g164120	CTACCCGCACAGGCCATATC	GTTGCTACTGCTCGATTGCG
BVRB_1g004050	AGGCGAAGCAGAAAGCTCTTA	TGTTGTTGGCCAATACTTGCG
BVRB_5g122000	AGGTTGAGCCAGTGCCAATC	ACTGCCTCTGTTGCTGATCG
BVRB_8g184430	TGTGGCGGTGGATCGAAAA	GCATTTCGGCCCGTTTTGT
BVRB_3g053290	CACAGAAATTGTCTCAGTTGGGC	GAAAGCCCGTTACAATCTCGC
BVRB_3g053300	TTGGCGATGTGGATCCTGTAG	TTTGCAGGCCCAACTGAGAC
BVRB_6g142690	TGACCGGAAATGTGGAACCAA	TGAGCAACGTAAGGGTAGGAC
BVRB_3g048110	CGTAGTATACCACCATCCGCC	TTGATGGCTGCTCCAGTGTC
BvGAPDH	GCTTTGAACGACCACTTCGC	ACGCCGAGAGCAACTTGAAC

Identification and relative expression profile of HIPPs gene family cadmium stress in sugar beet

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