Table 1

Primers for PCR in tea plant"

Fig. 1

Cloning and physicochemical characterization of CsMCC genes in tea plant (A): gel electrophoresis analysis of CsMCC genes; (B): chromosomal localization of CsMCC genes; (C): gene structural analysis of CsMCC and AtMCC1; (D): transmembrane structure prediction of CsMCC1 protein; (E): hydrophobicity prediction of CsMCC protein, values < 0 indicate hydrophilic and > 0 indicate hydrophobic."

Table 2

Physicochemical properties of CsMCC1 and CsMCC2 proteins in tea plant"

Fig. 2

Evolutionary tree of the HAT protein family (A) and plant MCC proteins (B) The evolutionary tree of MCC homologous proteins from Camellia sinensis (CsMCC1/2), Arabidopsis thaliana (AtMCC1), Solanum lycopersicum (Solyc03T002163), Zea mays (Zm00001d017691), Oryza sativa (LOC_Os02g46700), Vitis vinifera (VIT_216s0039g01810), Carya illinoinensis Pawnee (CiPaw.05G216100), Castanea dentata (Caden.12G113400), Cucumis sativus (Cucsa.318740), Gossypium darwinii (Godar.D08G277700, Godar.A08G263100), Capsella rubella (Carub.0003s0211), Eruca vesicaria (Eruve.2849s0002), Isatis tinctoria (Isati.1576s0019), Citrus clementina (Ciclev10021817m), and Populus trichocarpa (Potri.013G079900)."

Fig. 3

Characterization of the MCC protein sequences (A): the analysis of conserved motifs in CsMCC and AtMCC1 proteins; (B): the schematic representation for conserved structural domains of MCC proteins; (C): the multiple sequence alignment of plant MCC proteins."

Fig. 4

Spatial structure analysis of CsMCC1 and CsMCC2 proteins (A): protein secondary structure; (B): protein tertiary structure."

Fig. 5

Subcellular localization of CsMCC in Arabidopsis protoplasts"

Fig. 6

Cis-elements detected in the promoter of the CsMCC genes (A): promoter element distribution, different colors correspond to different elements in the figure below; (B): the heat map shows the number of promoter elements, and the gray square indicates that the elements couldn’t be detected."

Fig. 7

Spatial-specific expression analysis of CsMCC genes in tea plants (A): the relative expression level of CsMCC1 and CsMCC2 genes in different tissues of tea plants; (B)-(E): the relative expression level of CsMCC1 and CsMCC2 genes at different developmental stages of tea buds, leaves, flowers, and roots, respectively."

Fig. 8

Relative expression pattern of CsMCC genes under different abiotic stresses and phytohormones in tea plant (A): 25% PEG was used to simulate the arid environment of tea plants, and CK_P, PEG_24 h/48 h/72 h indicated that tea plants were treated with PEG for 0, 24, 48, and 72 h, respectively. (B): simulated salt stress of tea plants with 11.7 g L-1 NaCl, CK_N, NaCl_24 h/48 h/72 h indicated that tea plants were treated with NaCl for 0, 24, 48, and 72 h, respectively. (C): cold acclimation treatment, CK_C as control, CA1 for cold acclimation, CA2 for de-acclimated; (D): CK_M, MeJA_12 h/24 h/48 h/denoting 0, 12, 24, and 48 h treated with MeJA, respectively. (E): CK_G and GA3_24 h/48 h indicated that tea plants were treated 0, 24, and 48 h were with 100 mg L-1 GA3, respectively; (F): CK_I, IAA_24 h/48 h indicated that tea plants were treated 0, 24, and 48 h with 50 mg L-1 IAA, respectively. TBtools generated a heatmap with normalized log2-transformed values. Red indicates upward expression, while blue indicates downward expression, and the darker the color, the greater the change in expression."

Fig. 9

Functional interacting network of CsMCC proteins"