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Acta Agronomica Sinica ›› 2025, Vol. 51 ›› Issue (7): 1736-1746.doi: 10.3724/SP.J.1006.2025.44141

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and functional analysis of glutathione S-transferase gene IbGSTU7 in sweetpotato

YIN Yu-Meng1,**,WANG Yan-Nan1,**,KANG Zhi-He1,QIAO Shou-Chen1,BIAN Qian-Qian1,LI Ya-Wei1,CAO Guo-Zheng1,ZHAO Guo-Rui1,XU Dan-Dan2,YANG Yu-Feng1,*   

  1. 1 Cereal Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China; 2 Tongxu Academy of Agricultural Sciences, Kaifeng 475400, Henan, China
  • Received:2024-08-30 Revised:2025-04-27 Accepted:2025-04-27 Online:2025-07-12 Published:2025-05-07
  • Supported by:
    This study was supported by the China Agriculture Research System of MOF and MARA (CARS-10), the Special Fund for Henan Agriculture Research System (HARS-22-04-G1), the Outstanding Youth Science and Technology Fund of Henan Academy of Agricultural Sciences (2024YQ17), the Autonomous Innovation Project of Henan Academy of Agricultural Sciences (2024ZC014), and the Central Government Guides Local Funds for Scientific and Technological Development of Henan Province (Z20231811177). 

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Abstract:

In this study, the IbGSTU7 gene was cloned based on our previously obtained transcriptome data from a sweetpotato skin color mutant. The open reading frame (ORF) of IbGSTU7 is 660 bp in length and encodes a protein comprising 219 amino acids. Protein sequence analysis revealed that IbGSTU7 is an acidic, hydrophilic, and structurally stable protein, showing the closest phylogenetic relationship to its homolog in Ipomoea triloba, a wild relative of sweetpotato. Subcellular localization analysis indicated that IbGSTU7 is localized in the cytoplasm. Quantitative RT-PCR analysis demonstrated that IbGSTU7 is expressed across major sweetpotato tissues, with the highest transcript level observed in the skin of storage roots. Furthermore, its expression was inducible by drought, salt, and exogenous abscisic acid (ABA) treatments. The IbGSTU7 gene was subsequently overexpressed in sweetpotato using an Agrobacterium tumefaciens-mediated transformation method. In overexpression (OE) lines, anthocyanin content in the skin of storage roots and the expression levels of anthocyanin biosynthesis-related genes IbPAL and IbUFGT were significantly increased. Additionally, the OE plants exhibited enhanced tolerance to PEG-simulated drought stress, as evidenced by significant increases in fresh weight and root length compared to wild-type (WT) plants. Under drought conditions, OE lines showed a marked reduction in hydrogen peroxide (H2O2) levels, accompanied by significant upregulation of reactive oxygen species (ROS) scavenging-related genes IbMDHAR and IbPOD. These findings provide novel insights into the role of IbGSTU7 in regulating anthocyanin accumulation and improving drought stress tolerance in sweetpotato.

Key words: sweetpotato, IbGSTU7, overexpression, anthocyanin, drought resistance

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