Table 1

Primers used in this study"

Fig. 1

PCR amplification and sequence comparison of the GhCDN10 gene A: PCR amplification of the GhCDN10 gene; M: 5000 bp DNA marker; Lane 1: PCR products amplified using ‘86 III 72’ gDNA as template; Lane 2: PCR products amplified using ‘86 III 72 glandless’ gDNA as template. B: comparison of PCR sequences amplified from glanded and glandless cotton; a: gene sequence from TM-1 reference genome; b: gene sequence from PCR product using ‘86 III 72 glandless’ gDNA as a template; c: gene sequence from PCR product using ‘86 III 72’ gDNA as a template."

Fig. 2

Exon-intron structure, motif arrangment, LD of SNPs and haplotype in GhCDN10 genes A: GhCDN10 gene structure (exon-intron); B: LD between SNPs in GhCDN10 body; C: LD decay curve in the whole DNA segment of GhCDN10; D: haplotypes of GhCDN10; E: information on SNPs in the DNA body of GhCDN10."

Table 2

Features of DNA sequence of GhCDN10"

Fig. 3

Cluster analysis of 32 germplasms based on SNPs within the gene body of GhCDN10 Black and red indicates normal accessions and low-gossypol germplasms, respectively."

Fig. 4

Phylogenetic tree (A) based on amino acid sequences, motif arrangement (B) and exon-intron structure (C) of GhCDN10"

Fig. 5

Secondary structure (A) and tertiary structure (B) of GhCDN10 protein Blue: α-helix; Red: β-turn; Green: extended strand; Purple: random coil."

Fig. 6

Expression profiles of the GhCDN10 gene A: expression pattern of GhCDN10 in G. hirsutum (glanded cotton and glandless cotton) embryo (14, 16, 32 DPA) (RNA-Seq); B: expression pattern in G. hirsutum (glanded cotton and glandless cotton) leaf (RNA-Seq); C: expression pattern in diverse tissues, developmental stages of ovule and fiber (RNA-Seq); D: expression pattern of upland cotton roots under light and dark conditions (RNA-Seq); E: expression pattern of roots and stems of glanded cotton TM-1 and TM-1_VIGS plants, and of glandless cotton T582 (RNA-Seq); F: expression pattern of leaves and ovules of glanded cotton and glandless cotton (RNA-Seq); G: gene expression abundance from qRT-PCR assays; H: expression pattern validation; Bar: expression levels in qRT-PCR assay; Line: abundance of gene expression in RNA-Seq data. Different lowercase letters indicate significant difference at the 0.05 probability level. DPA: days post-anthesis."

Fig. 7

GO term (A) and KEGG pathway (B) enrichment of genes correlated with GhCDN10 in expression profiles"

Fig. 8

VIGS analysis of the GhCDN10 gene A: PCR amplification of VIGS fragment of GhCDN10; M: 2000 bp DNA marker; 1: PCR product; B, C: cotton phynotype after VIGS and the silencing effect (albino leaf) of PDS gene in cotton; D, E: plant leaves inoculated with Agrobacterium carrying TRV2::GUS and TRV2::GhCDN10 vectors respectively (illustrating quantity and density of gland); F: qRT-PCR expression level of GhCDN10 gene after VIGS; G: number of glands on leaves after VIGS; H: gossypol content of leaves after VIGS; I, J: liquid chromatography profile of gossypol (Left: gossypol standard, Right: tested sample). Different lowercase letters indicate significant differences at P < 0.05."

Fig. 9

Subcellular localization of the GhCDN10-GFP fusion protein"