Table 1

Functional markers and sequencing primers designed based on sequence differences"

Fig. 1

Sequence alignment of the functional locus of the STV11 gene in resistant rice varieties The picture shows the sequence comparison results of Huaidao 5, Nanjing 45, Nanjing 46, Nanjing 5055, Nanjing 9108, Nongxiang 39, Yuehesimiao, and Zhendao 88. The gray frame shows the difference of resistant varieties."

Fig. 2

Polymorphism analysis of the STV11 gene using high-resolution melting (HRM) functional markers The horizontal axis denotes temperature, while the vertical axis corresponds to the fluorescence emission intensity measured between 465 nm and 510 nm. A: test result of stvHRM-1; B: result of stvHRM-2 detection; C: test result of stvHRM-4 detection; D: test result of stvHRM-5; E: result of stvHRM-3 detection. Blue shows the melting curves of Zhendao 88, Nanjing 46, Nanjing 5055, Nanjing 9108, Nongxiang 39 and Yuehesimiao. Red shows the melting curves of Huaidao 5 and Nanjing 45. Gray shows the melting curve of mixed DNA of resistant varieties. STV11-R represents the disease-resistant genotype, STV11-S represents the disease-susceptible genotype, and Heterozygote represents the heterozygous type."

Fig. 3

Identification of STV11 genotype of intermediate material for rice breeding Abbreviations are the same as those given in Fig. 2. Blue represents the melting curves of Linghe 9178, Nongxiang 21, Habo 3003, Fan 20, R6547, Huazhan, Baohuang 33145, Xingdao 99, and Suxiu 867. Red represents the melting curves of Xudao 268, Jinwujing 3087, Huajing 0061, Ningjing 2243, Chunjing 747, Tianfengjing 98, Xudao 3, and Huazhejing 20124. Green represents the melting curves of Jindanjing 125, Huaidao 5/Runyangdao 17001 (Pigm) (F7), Wuyunjing 39//Suifan-20 ([53045/07GY31(pigm)BC1F2] /Huaidao 5 (fast grain-filling) (F5)) (BC1F1) (F5), Zijingnuo 1 Xiang/A120-41 (F8), Baofeng 246, and Jinjing 59/ ([9108/NJ45HR (F2)]/71029 (F7)) (F5)."

Fig. 4

Sequencing peaks of STV11 gene in rice materials identified by stvHRM-3 marker Abbreviations are the same as those given in Fig. 2. A to C show the sequencing chromatograms of the STV11 gene from single plants of Linghe 9178, Huajing 0061, and Huaidao 5/Runyangdao 17001 (Pigm) (F7), respectively. The red box indicates nucleotides 773-779 of the STV11 gene."

Table 2

Detection and phenotype of resistance genotypes to stripe leaf blight in Jiangsu province"

Table 3

Comparison of resistance identification results for rice stripe virus using the molecular markers stvHRM-3 and ST10"

Fig. 5

Detection of the molecular marker ST10 in late-ripening medium japonica identification materials The presence of a band indicates the resistant genotype, while its absence denotes the susceptible genotype. Numbers in this figure correspond to the materials was listed in Table S1."

Table S1

Hybrid combination of partial late-maturing medium japonica evaluation lines in the evaluation"

Table 4

HRM-PCR analysis of STV11 genotypes of 520 rice accessions"