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    12 September 2025, Volume 51 Issue 9
    • REVIEW
      Research advances on plant midday depression of photosynthesis
      GAO Yuan, LI Xia, WEI Shao-Bo, TIAN Xiao-Hai, ZHOU Wen-Bin
      Acta Agronomica Sinica. 2025, 51(9):  2253-2265.  doi:10.3724/SP.J.1006.2025.53033
      Abstract ( 199 )   HTML ( 27 )   PDF (709KB) ( 82 )   Save
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      The midday depression of plant photosynthesis refers to the phenomenon that plants experience a decline in photosynthetic efficiency around noon under natural conditions, followed by a subsequent recovery. This phenomenon was first identified over a century ago, yet its underlying mechanisms remain unclear. It not only affects the plant photosynthetic productivity, but also plays an important role in the yield formation of field crops. Therefore, exploring the physiological and molecular mechanisms of photosynthetic midday depression is of great theoretical and practical importance for improving the crop photosynthetic efficiency and increasing yields. This review reviewed the discovery process of photosynthetic midday depression, the environmental factors affecting the formation of photosynthetic midday depression and the related physiological regulation mechanism. Additionally, we discussed and provided perspectives on the future research directions, aiming to offer insights and references for future studies in this field.

      CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
      Genome-wide association analysis of stomatal-related traits in wheat leaves
      LI Lu-Qi, CHENG Yu-Kun, BAI Bin, LEI Bin, GENG Hong-Wei
      Acta Agronomica Sinica. 2025, 51(9):  2266-2284.  doi:10.3724/SP.J.1006.2025.51028
      Abstract ( 144 )   HTML ( 15 )   PDF (11500KB) ( 71 )   Save
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      Wheat stomata are microscopic pores that regulate photosynthesis and transpiration, playing a critical role in determining yield. Understanding the genetic mechanisms underlying stomatal traits and identifying candidate genes associated with these characteristics are essential for improving wheat productivity. In this study, 276 winter wheat varieties (lines) were evaluated under two irrigation treatments—normal irrigation and drought stress. Stomatal traits, including stomatal density, average stomatal area, and stomatal pore proportion, were measured at both the heading and filling stages. A genome-wide association study (GWAS) was conducted using a 90K wheat SNP chip to analyze these traits. The results revealed significant phenotypic variation in stomatal traits across the two irrigation regimes and developmental stages, with coefficients of variation ranging from 0.06 to 0.28. GWAS identified 88 loci significantly associated with stomatal traits across 20 chromosomes, excluding chromosome 4D (P < 0.001). Among these, four stable genetic loci related to average stomatal area were identified on chromosomes 1B, 3A, and 6A, explaining 2.78% to 6.55% of the phenotypic variance. These loci were all detected during the filling stage under normal irrigation. Two loci associated with stomatal density were identified at both the heading and filling stages: Ex_c69429_328 on chromosome 6A under normal irrigation explained 2.31% to 3.06% of the variation, while BS00064423_51 on chromosome 4A under drought stress accounted for 4.40% to 6.09%. Additionally, eight loci with pleiotropic effects were identified on chromosomes 1A, 1B, 3A, 4A, 5A, 6A, and 6D, explaining 1.25% to 7.31% of the phenotypic variation. Haplotype analysis was performed on loci with a contribution rate greater than 5.00% that were detected in at least two environments or associated with two or more traits. Notably, the wsnp-Ex_rep_c69627_68580121 locus (R2 = 6.47%), significantly associated with both average stomatal area and stomatal pore proportion, exhibited two haplotypes—Hap1 and Hap2. Among the 276 wheat lines, those carrying Hap1 (frequency: 81.20%) had a significantly smaller average stomatal area than those with Hap2 (frequency: 18.80%) (P < 0.05). Haplotype distribution varied across wheat-growing regions, with Hap1 most prevalent in the southwest winter wheat region, while Hap2 was more common in northern regions. A total of nine candidate genes associated with stomatal traits were identified based on loci detected in multiple environments and those with pleiotropic effects. These genes are likely involved in stomatal development, photosynthesis, and stress responses, offering promising targets for further research and genetic improvement of wheat stomatal traits.

      CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
      Establishment of regeneration system and creation of glufosinate-resistant germplasm for early-mid maturing potato ‘Longshu 20’
      JIA Xiao-Xia, QI En-Fang, WEN Guo-Hong, MA Sheng, HUANG Wei, LYU He-Ping, LI Jian-Wu, QU Ya-Ying, DING Ning
      Acta Agronomica Sinica. 2025, 51(9):  2285-2294.  doi:10.3724/SP.J.1006.2025.54024
      Abstract ( 87 )   HTML ( 14 )   PDF (12095KB) ( 28 )   Save
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      To establish an efficient regeneration system for the new early-mid maturing, vegetable-use potato cultivar ‘Longshu 20’ and to develop novel germplasm resistant to glufosinate, we systematically evaluated the embryogenic callus induction efficiency and shoot regeneration capacity of test-tube plantlet stem segments cultured on MS medium supplemented with varying concentrations and combinations of 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA), and gibberellic acid (GA3). The highest rates of embryogenic callus induction and shoot differentiation were observed on medium MS + 1.0 mg L-1 6-BA + 0.5 mg L-1 NAA + 4.5 mg L-1 GA3 + 3% sucrose. On day 30, the embryogenic callus induction rate reached 89.83%, while the shoot differentiation rate reached 91.81% by day 45. A Bar gene overexpression vector was constructed using recombinant DNA technology and introduced into stem segments via Agrobacterium-mediated transformation. After selection on MS medium containing 2.0 mg L-1 phosphinothricin (PPT), six independent transgenic lines were successfully obtained. Both transgenic and non-transgenic control lines were grown and treated with commercial glufosinate-ammonium at an active ingredient content of 1271 g hm-2 during the seedling stage. Within 9 days, all non-transgenic control plants had completely withered and died, while all transgenic lines exhibited stable resistance to glufosinate-ammonium. Phenotypic analysis at maturity revealed variation among transgenic lines in terms of tuber number per plant, yield per plant, and key quality traits. Notably, transgenic line S20-4 showed no significant differences from the non-transgenic control in tuber number, yield, or key quality parameters such as starch, crude protein, and reducing sugar content. These results demonstrate that S20-4 successfully integrates glufosinate resistance while concurrently maintaining the yield and quality traits of the recipient cultivar. The efficient regeneration system developed in this study provides a solid foundation for the genetic improvement of ‘Longshu 20’, and the transgenic line S20-4 represents a promising candidate for direct application in breeding programs targeting herbicide resistance and high yield.

      CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
      Effects of waterlogging stress on root metabolism of maize seedlings
      JIANG Huan-Qi, DUAN Ao, GUO Chao, HUANG Xiao-Meng, AI De-Jun, LIU Xiao-Xue, TAN Jing-Yi, PENG Cheng-Lin, LI Man-Fei, DU He-Wei
      Acta Agronomica Sinica. 2025, 51(9):  2295-2306.  doi:10.3724/SP.J.1006.2025.53016
      Abstract ( 126 )   HTML ( 2 )   PDF (9159KB) ( 62 )   Save
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      To investigate key metabolites and changes in metabolic pathways in maize (Zea mays L.) seedlings under waterlogging stress, plants were subjected to four stress durations (0, 1, 4, and 7 days). Transcriptome sequencing (RNA-seq) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were employed to identify differentially accumulated metabolites, followed by KEGG enrichment analysis to explore associated metabolic pathways. A total of 1361 differential metabolites were annotated and quantified. The most pronounced metabolic changes were observed at 4 days, with 414 metabolites significantly altered—372 upregulated and 42 downregulated. The top 20 most differentially accumulated metabolites were all upregulated, including ferulic acid-4-O-glucoside, 2-phenylethanol, 7-methyl-ergochalcone, and S-allyl-L-cysteine. KEGG pathway analysis revealed significant enrichment in flavonoid biosynthesis, carotenoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signaling, ABC transporters, fatty acid degradation, starch and sucrose metabolism, glycine, serine and threonine metabolism, tryptophan metabolism, and glycolysis/gluconeogenesis. Among these, flavonoid biosynthesis was found to be closely associated with waterlogging stress, with key flavonoids such as naringenin and luteolin, and enzymes including chalcone isomerase, flavonoid synthase II, and flavonoid 3',5'-hydroxylase/3'-monooxygenase playing critical roles. These findings provide new insights into the molecular mechanisms underlying maize tolerance to waterlogging and offer a theoretical basis for breeding waterlogging-tolerant maize varieties.

      Screening of potato germplasm for resistance to Meloidogyne incognita and analysis of related physiological responses
      ZHU Jin-Cheng, YANG Qiu-Hua, CHENG Li-Xiang, LI Wen-Li, SHI Ming-Ming, LI Hui-Xia, ZHANG Feng
      Acta Agronomica Sinica. 2025, 51(9):  2307-2317.  doi:10.3724/SP.J.1006.2025.54001
      Abstract ( 64 )   HTML ( 3 )   PDF (3966KB) ( 15 )   Save
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      This study aimed to identify potato germplasm with resistance to Meloidogyne incognita and to analyze their physiological defense responses, thereby providing a theoretical basis for breeding nematode-resistant potato varieties and enriching pest-resistant germplasm resources. Molecular identification of nematodes was performed using the universal primer pair D2A/D3B and the M. incognita-specific primer pair Inc-K14-F/Inc-K14-R. A total of 54 wild potato introgression lines and 31 cultivated varieties were inoculated with M. incognita, and the numbers of egg masses and root galls were recorded 35 days after inoculation. The egg index (EI), gall index (GI), and disease index (DI) were calculated to evaluate resistance. Following preliminary screening, non-inoculated resistant and susceptible materials were used as controls, and the contents of lignin, jasmonic acid (JA), and salicylic acid (SA) in roots were measured at 3, 7, and 35 days post-inoculation to analyze the physiological responses of resistant potato root systems to M. incognita. The results revealed significant variation in egg mass and root gall numbers, EI, and GI among the 85 potato genotypes. Based on DI values, materials were categorized into four resistance levels: highly resistant (1 < DI ≤ 2), moderately susceptible (4 < DI ≤ 5), susceptible (5 < DI ≤ 6), and highly susceptible (DI > 6). The wild introgression line 232-8 was identified as highly resistant (DI = 1.01); lines 232-9 (DI = 4.02), 315-53 (DI = 4.09), and 390-10 (DI = 4.33) were moderately susceptible; while lines 364-3 (DI = 5.17), 19-2 (DI = 5.28), 53-1 (DI = 5.88), and 317-8 (DI = 5.88) were susceptible. The remaining 77 genotypes were highly susceptible, with DI values ranging from 6.13 to 74.26; Tianshu 12 showed the highest DI (74.26). Following M. incognita inoculation, line 232-8 exhibited significantly higher root contents of lignin, JA, and SA compared with Tianshu 12. Lignin content peaked at 35 days post-inoculation (476.18 mg g-1), while JA and SA contents peaked at 7 days (10.80 ng g-1 and 1623.15 ng g-1, respectively). These results suggest that wild potato introgression line 232-8 is resistant to M. incognita, and the significant induction of lignin, JA, and SA following nematode invasion may contribute to its physiological resistance mechanisms.

      Identification and functional analysis of low temperature responsive genes ZmNTL1 and ZmNTL5 in maize
      GAO Yuan, WANG Yu-Qi, JIANG Jia-Ning, ZHAO Jian-Xiong, WANG Xue-He-Yuan, WANG Hao-Yu, ZHANG Rui-Jia, XU Jing-Yu, HE Lin
      Acta Agronomica Sinica. 2025, 51(9):  2318-2329.  doi:10.3724/SP.J.1006.2025.53011
      Abstract ( 92 )   HTML ( 8 )   PDF (12035KB) ( 41 )   Save
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      Low temperature is one of the major abiotic stresses limiting maize yield in Northeast China. Membrane-bound NAC transcription factors play important roles in plant stress responses. Investigating their function provides a theoretical basis for breeding maize with enhanced cold tolerance. In this study, the molecular characteristics of nine maize NTL genes were analyzed using bioinformatics approaches. Analysis of publicly available RNA-seq data revealed that six of these genes were significantly upregulated by low temperature stress in both roots and leaves. Among them, ZmNTL1 and ZmNTL5, which share 80.04% sequence homology, were selected for further study. A double knockout mutant (zmntl1zmntl5) was generated using gene-editing techniques. Under low temperature stress, the ion leakage rate in the leaves of zmntl1zmntl5 was significantly higher than in the wild-type B104. The fresh and dry weights of both aboveground and belowground tissues, as well as chlorophyll content, were significantly lower in zmntl1zmntl5 compared to B104. In contrast, zmntl1zmntl5 exhibited significantly higher levels of O2- and H2O2, while the activities of antioxidant enzymes GST, SOD, POD, and CAT were significantly reduced. Furthermore, the expression levels of ZmGST24, ZmNCED3, ZmDREB1.6, and ZmDREB2A were markedly decreased in zmntl1zmntl5 compared to B104 under cold stress. In summary, the absence of ZmNTL1 and ZmNTL5 compromises cold tolerance in maize, suggesting that both genes function as positive regulators in the cold stress response. This study provides a valuable theoretical foundation and genetic resources for the improvement of cold-tolerant maize varieties and the development of new cultivars.

      Development and application of functional insertion and deletion (InDel) markers associated with maize Waxy gene compatible with dual-platform
      ZHU Wei-Jia, WANG Rui, XUE Ying-Jie, TIAN Hong-Li, FAN Ya-Ming, WANG Lu, LI Song, XU Li, LU Bai-Shan, SHI Ya-Xing, YI Hong-Mei, LU Da-Lei, YANG Yang, WANG Feng-Ge
      Acta Agronomica Sinica. 2025, 51(9):  2330-2340.  doi:10.3724/SP.J.1006.2025.53013
      Abstract ( 75 )   HTML ( 0 )   PDF (5555KB) ( 19 )   Save
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      To enable rapid identification of variation types associated with the Waxy gene in waxy maize and to support its application in modern waxy maize breeding, we developed functional markers targeting four common InDel variations of the Waxy gene: wx-D7, wx-D10, wx-124, and wx-hAT. The specificity and effectiveness of these markers were validated across multiple molecular detection platforms, including in common maize, waxy maize, sweet maize, and sweet-waxy maize. Results showed that the four Waxy-associated functional markers enabled specific genotyping on both the KASP and fluorescence capillary electrophoresis platforms. These markers effectively distinguished common maize from waxy maize and identified the Waxy gene variation types in waxy maize lines. The waxy phenotype could be inferred based on the Waxy gene haplotype when specific functional markers were detected in inbred lines. Maize germplasms lacking these four waxy variations exhibited either non-waxy phenotypes or rare waxy variants. For hybrid maize samples, four possible genotypic combinations were observed based on Waxy haplotype analysis: recessive homozygous, recessive allele heterozygous, waxy/non-waxy heterozygous, and dominant homozygous genotypes. Notably, over 85% of waxy maize carried the wx-D7 variation, indicating that wx-D7 is the predominant allele used in modern waxy maize breeding in China. Additionally, we found that multiple Waxy gene variations, such as D7/D10, coexisted in waxy maize hybrids, while only a single variation type was present in waxy inbred lines. This suggests that the aggregation of different Waxy variations may contribute to genetic improvement in waxy maize breeding. In summary, we developed a set of functional markers for the Waxy gene that are compatible with multiple molecular detection platforms, providing an efficient tool for the identification and screening of waxy maize germplasm.

      Cloning and functional analysis of HvERF039 gene in Qingke (Hordeum vulgare L. var. nudum Hook. f)
      MA Juan-E, YAO You-Hua, YAO Xiao-Hua, WU Kun-Lun, CUI Yong-Mei
      Acta Agronomica Sinica. 2025, 51(9):  2341-2357.  doi:10.3724/SP.J.1006.2025.51011
      Abstract ( 116 )   HTML ( 3 )   PDF (16174KB) ( 28 )   Save
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      AP2/ERF (APETALA2/ethylene responsive factor) transcription factors play crucial roles in responses to abiotic stresses, but their functions remain poorly understood in Qingke (hulless barley). In this study, we cloned the HvERF039 gene and investigated its role in cold stress response through bioinformatics analysis, qRT-PCR, and heterologous expression in Arabidopsis thaliana. HvERF039 encoded a hydrophilic, unstable protein containing a typical AP2 conserved domain. The promoter region of HvERF039 contained various cis-acting elements, including those responsive to light, hormones and cold stress. Subcellular localization and transactivation assays revealed that HvERF039 protein was localized in both the membrane and nucleus and possessed transcriptional activation activity. qRT-PCR analysis showed that HvERF039 expression was significantly upregulated under cold treatment, with the highest expression observed in leaves and detectable levels in most other tissues. Functional analysis demonstrated that transgenic Arabidopsis plants overexpressing HvERF039 exhibited significantly higher germination and survival rates under cold stress compared to wild-type plants. These transgenic lines also showed enhanced physiological resistance, including lower ion leakage, reduced H2O2 and MDA contents, and increased CAT activity. Furthermore, HvERF039 was found to physically interact with multiple stress-related proteins. Collectively, these findings suggest that HvERF039 plays a positive regulatory role in cold stress tolerance and represents a promising genetic resource for improving cold resistance in Qingke cultivars.

      Genetic diversity analysis of domestic albino tea germplasm resources based on the tea plant liquid phase functional chip
      MEI Piao, LIU Ding-Ding, YE Yuan-Yuan, ZHANG Chen-Yu, DING Shi-Qi, LI Ya-Qi, WANG Pei-Xin, MEI Ju-Fen, MA Chun-Lei
      Acta Agronomica Sinica. 2025, 51(9):  2358-2370.  doi:10.3724/SP.J.1006.2025.54043
      Abstract ( 118 )   HTML ( 0 )   PDF (10060KB) ( 27 )   Save
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      In this study, a self-developed high-density liquid-phase functional chip for tea plants was employed to genotype the major albino tea germplasms in China. Based on genetic similarity analyses, 61 core accessions were selected for genetic diversity assessment. Phylogenetic tree construction and population structure analysis revealed that these albino tea resources could be classified into three main groups, with their distribution closely associated with geographical origins and breeding histories. Principal Component Analysis further indicated that most albino tea accessions in China originate from Zhejiang province. Compared with the rich genetic resources of conventional tea germplasms in China, albino tea plants exhibit relatively limited genetic diversity, underscoring the need and potential for genetic improvement through breeding. Additionally, the quality components of 30 representative albino tea accessions were analyzed. Caffeine content ranged from 2.26% to 4.17%, with an average of 3.51%; total amino acid content varied from 1.85% to 7.54%, with a mean of 4.33%; and total catechin content ranged from 8.63% to 16.68%, averaging 13.28%. Compared to conventional green tea cultivars, most albino tea accessions exhibited higher amino acid levels and lower alkaloid and catechin contents, making them promising raw materials for the production of high-quality green tea. In conclusion, this study provides a comprehensive analysis of the genetic structure and diversity of major albino tea germplasms in China and demonstrates the effectiveness of the liquid-phase functional chip in tea germplasm and cultivar identification. These findings lay a theoretical foundation for the innovative utilization and genetic improvement of albino tea resources.

      Screening of drought and salt tolerant germplasm during wheat seedling stage and comprehensive evaluation of drought and salt tolerance
      HU Run-Hui, WANG Jun-Cheng, SI Er-Jing, ZHANG Hong, LI Xing-Mao, MA Xiao-Le, MENG Ya-Xiong, WANG Hua-Jun, LIU Qing, YAO Li-Rong, LI Bao-Chun
      Acta Agronomica Sinica. 2025, 51(9):  2371-2386.  doi:10.3724/SP.J.1006.2025.51022
      Abstract ( 176 )   HTML ( 2 )   PDF (2526KB) ( 71 )   Save
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      A drought and salt stress evaluation system was established to screen for drought- and salt-tolerant wheat germplasm. Eight wheat genotypes (varieties/lines) were used as experimental materials, and hydroponic culture was employed at the seedling stage. Plants were subjected to drought stress (20% PEG-6000), salt stress (200 mmol L-1 NaCl), and combined stress (20% PEG-6000+200 mmol L-1 NaCl). A total of 19 traits, including biomass, root-related parameters, and physiological indices, were measured under each stress condition. Drought and salt tolerance indices were calculated for each trait, followed by principal component analysis (PCA) and cluster analysis using the comprehensive membership function method to evaluate the stress tolerance of the different wheat varieties. Compared with the control, leaf relative water content decreased to varying degrees under all three stress treatments. In contrast, protective enzyme activities (SOD, POD, and CAT), membrane lipid peroxidation (MDA content), and proline accumulation showed overall increases. Soluble protein content declined across the different wheat varieties. Root morphological indicators such as average root volume, root surface area, and total root length generally increased under stress. The coefficient of variation among traits reached up to 116.86% across treatments. PCA was performed on the drought and salt tolerance indices of the 19 traits, and the comprehensive evaluation index (D value) was calculated using the membership function method. Multiple regression analysis identified chlorophyll content (SPAD), soluble protein content (SP), root surface area, average root volume, total root length, and root-to-crown ratio as key indicators for evaluating drought and salt tolerance in wheat lines. Systematic cluster analysis further revealed that Xinong 535, Longyu 11, Lan 19, Lantian 10, and Longzimai 1 exhibited strong drought resistance; Longjian 114 and Xikemai 510 showed strong salt tolerance; and Xinong 535 and Longyu 11 performed best under combined drought and salt stress.

      Genome-wide association analysis of drought resistance traits in wheat seedlings introduced from ICARDA
      LI Yun-Xiang, GUO Qian-Qian, HOU Wan-Wei, ZHANG Xiao-Juan
      Acta Agronomica Sinica. 2025, 51(9):  2387-2398.  doi:10.3724/SP.J.1006.2025.51019
      Abstract ( 88 )   HTML ( 2 )   PDF (5545KB) ( 23 )   Save
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      Wheat is one of the most important staple crops globally, and drought stress can severely impact its growth and development. Therefore, understanding the genetic basis of drought tolerance in wheat and identifying superior drought-resistance- related genes is of great importance for ensuring national food security. In this study, 159 wheat accessions introduced from ICARDA were used as experimental materials. A 20% PEG-6000 solution was applied to simulate drought conditions at the seedling stage in hydroponic experiments. Phenotypic data were collected for five root traits—total root length, root surface area, root volume, average root diameter, and root fork number. Correlation analysis was conducted, and drought tolerance coefficients for the five traits were calculated using data obtained from a 55K SNP chip. The results revealed substantial phenotypic variation in root traits under both control and drought conditions. Under normal conditions, the coefficients of variation ranged from 27.10% to 40.46%, while under drought stress they ranged from 24.95% to 57.04%. Correlation analysis of the drought tolerance coefficients showed no significant relationship between the coefficient for average root diameter and those for root surface area and root fork number. However, a significant negative correlation was observed between average root diameter and total root length, while the other traits exhibited significant positive correlations with each other. Genome-wide association analysis (GWAS) identified a total of 39 SNP loci significantly associated with root traits at the P ≤ 0.001 level. These loci were distributed across 16 chromosomes (1B, 1D, 2B, 3A, 3B, 3D, 4A, 4B, 4D, 5A, 5B, 6A, 6D, 7A, 7B, and 7D), with explained phenotypic variation ranging from 7.12% to 14.44%. Six pleiotropic loci were identified, all significantly associated with both root surface area and total root length, and located on chromosomes 3B and 4A, with contribution rates ranging from 7.15% to 14.44%. Based on these 39 significant loci, candidate gene prediction identified 12 genes potentially related to drought tolerance in wheat. Among them, TraesCS5B01G556300 (MYB transcription factor 60), TraesCS7A01G508700 (WRKY transcription factor WRKY28), TraesCS2B01G002700 (dehydration-responsive element-binding protein 1C), and TraesCS3D01G055500 (14-3-3-like protein) are likely to play important roles in regulating drought tolerance in wheat.

      Comparison of wound healing capacity of tubers of different potato varieties
      YIN Li-Na, ZHANG Rui, CHEN Guo-Huan, BAI Lei, LI Jun, GUO Hua-Chun, YANG Fang
      Acta Agronomica Sinica. 2025, 51(9):  2399-2411.  doi:10.3724/SP.J.1006.2025.54027
      Abstract ( 73 )   HTML ( 0 )   PDF (9233KB) ( 10 )   Save
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      The wound healing ability of potato tubers varies among cultivars. In this study, five tetraploid potato cultivars ‘Dianshu 47’ (D47), ‘Dianshu 14023’ (14023), ‘Dianshu 1208’ (1208), ‘Cooperation-88’ (C88), and ‘Qingshu 9’ (Q9) were used, as experimental materials. The accumulation of suberin and lignin at wound sites was observed, along with the activity of key enzymes and metabolite contents involved in the phenylpropanoid pathway, as well as the relative expression levels of genes related to suberin biosynthesis. The wound healing capacity of the five cultivars was comprehensively evaluated using the membership function method. The results showed that the activities of PAL, 4CL, C4H, and POD, as well as the relative expression levels of StPAL, StPOD, St4CL, and StC4H, increased rapidly during the early stage of wound healing (0-2 d) and peaked during the middle to late stages (4-8 d). As healing progressed, the contents of lignin, flavonoids, and total phenolics increased, while SPP and SPA were rapidly deposited to form the wound periderm. Among the whole cultivars, D47 exhibited, the lowest weight loss rate, 1208 and C88 showed the highest. The deposition rates of SPA, SPP, and lignin layers in D47 were relatively rapid, reaching maximum levels at 6 days post-injury, while deposition in 14023 and 1208 was comparatively slower. Additionally, D47 and C88 showed higher activities of PAL, POD, and C4H, and therelative expression levels of StPAL, StPOD, and StC4H were significantly higher in D47 and Q9 than in the other cultivars (P < 0.05), In contrast, 14023 and 1208 exhibited lower PAL and POD activities. During the wound-healing process, lignin, total phenolics, and flavonoid contents were consistently higher in D47 and lower in 1208 and 14023. Principal component analysis of 18 wound-healing indicators across the 0-8 day period identified weight loss rate, SPA cell layer thickness, SPP cell layer thickness, lignin layer thickness, and the relative expression levels of StPAL, StC4H, and StPOD as representative indicators for evaluating wound healing ability. Based on membership function analysis, the overall ranking of wound healing ability was: D47 (3.9808) > Q9 (3.5767) > C88 (3.4663) > 1208 (3.3546) > 14023 (2.0241). These findings suggest that the phenylpropanoid metabolic pathway plays a key role in the wound suberization process of potato tubers.

      Differences and correlations in physiological and molecular mechanisms of barley germination under drought stress
      HE Peng-Xu, YAO Li-Rong, CHEN Yuan-Ling, YAN Yan, ZHANG Hong, WANG Jun-Cheng, LI Bao-Chun, YANG Ke, SI Er-Jing, MENG Ya-Xiong, MA Xiao-Le, WANG Hua-Jun
      Acta Agronomica Sinica. 2025, 51(9):  2412-2432.  doi:10.3724/SP.J.1006.2025.41079
      Abstract ( 87 )   HTML ( 2 )   PDF (4877KB) ( 36 )   Save
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      Drought is a major abiotic stress affecting the growth and development of barley (Hordeum vulgare L.), with potentially irreversible impacts across all growth stages, including seed germination and flowering. To investigate the germination responses of diverse barley germplasm under drought stress, this study employed a vermiculite-based water control method to evaluate the germination characteristics of 54 barley germplasm accessions. Based on drought tolerance during germination, the accessions were classified into five categories: excellent, good, moderate, poor, and very poor, with the highest proportion (37.04%) falling into the moderate group. Seven accessions with strong drought tolerance during germination, such as E0270389 and ZDM5430, exhibited rapid germination, uniform emergence, and vigorous seedling growth. In contrast, six accessions with poor drought tolerance, including 08 Jing 134 and Ziyuan136-21490058, showed low germination rates, asynchronous emergence, and poor seedling uniformity. Six representative accessions (E0270389, ZDM5430, 7DCADA, Z1920057W, Feite 36, and ZY69-G231M004M) with significantly different drought germination characteristics were further analyzed for physiological and biochemical parameters, as well as DNA damage repair mechanisms during germination under drought conditions. The results revealed that accessions with good drought tolerance had lower expression of HvFPG during mid-germination and lower HvOGG1 expression during early germination compared to drought-sensitive accessions. In addition, these drought-tolerant accessions maintained higher peroxidase (POD) activity during early and middle germination stages, and showed less reduction in superoxide dismutase (SOD) and cysteine protease activities before germination. Soluble sugar content at 6 hours after drought induction did not differ significantly from the control. In contrast, drought-sensitive accessions exhibited significantly reduced POD activity during mid-germination and a marked decrease in soluble sugar content, as observed in Feite 36. In summary, barley germplasm with good drought tolerance during germination demonstrated enhanced DNA repair capacity, antioxidant activity, and metabolic adaptability under drought stress, leading to faster germination and healthier seedling development.

      TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
      Population dynamic characteristics and formation mechanisms of super high-yielding of two types of glutinous rice in the middle and lower reaches of the Yangtze Rive
      GUO Bao-Wei, WANG Wang, WANG Kai, WANG Yan, ZENG Xin, JING Xiu, WANG Jing, NI Xin-Hua, XU Ke, ZHANG Hong-Cheng
      Acta Agronomica Sinica. 2025, 51(9):  2433-2453.  doi:10.3724/SP.J.1006.2025.52003
      Abstract ( 102 )   HTML ( 1 )   PDF (2007KB) ( 32 )   Save
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      To investigate the characteristics and formation patterns of super high-yielding (SHY) populations in glutinous rice and to provide theoretical guidance for high-yield cultivation, we used three glutinous rice cultivars as experimental materials: the conventional japonica variety Yangjingnuo 2 and the indica-japonica hybrid varieties Shuyounuo 82 and Shuyounuo 85. We analyzed the photosynthetic production and translocation of assimilates, population structure, lodging resistance, and yield performance across high-yielding (HY), higher-yielding (HRY), and super high-yielding (SHY) glutinous rice populations to clarify their characteristics and formation mechanisms. The results showed as follows: (1) Compared with HY and HRY populations, the SHY population had a significantly higher total spikelet number, while 1000-grain weight and seed setting rate were slightly lower, but not significantly different. SHY populations were characterized by large panicles and high total spikelet numbers (exceeding 43,000×104 hm-2 in conventional japonica and 60,000×104 hm-2 in hybrid types), while maintaining stable 1000-grain weight and seed setting rate. (2) From the effective critical leaf age to the jointing stage, there were no significant differences in leaf area index (LAI) or photosynthetic potential among yield types. However, from heading to maturity, both LAI and photosynthetic potential followed the order SHY > HRY > HY, while leaf area decay rate showed the opposite trend. Differences among the three yield types were highly significant during this period. (3) No significant differences in dry matter accumulation were observed from the critical leaf age to the booting stage. However, from heading to maturity, SHY populations accumulated significantly more dry matter than HRY and HY. During the booting to milky stage, SHY also showed a significantly higher dry matter accumulation rate. From the milky to waxy stage, the accumulation rate did not differ significantly in the conventional japonica type, but in the hybrid indica-japonica type, SHY populations still exhibited a significantly higher rate than HRY and HY. (4) With increasing yield grade, the top three leaves became longer, with smaller opening and base angles and reduced drooping; plant height increased, and the plant architecture became more upright. The first, second, and third basal internodes increased significantly in length, thickness, and width, contributing to enhanced stem strength and lodging resistance. Except for the third internode in conventional glutinous rice, the lodging index significantly decreased with higher yield grades. (5) In both glutinous rice types, population yield was significantly correlated with LAI at heading, dry matter weight at heading and maturity, and net dry matter accumulation. A strong positive correlation was also found between yield and LAI at maturity. SHY populations possessed a large and stable sink capacity (panicle size), a strong photosynthetic source capacity after panicle initiation, and an upright plant type with high stem strength and lodging resistance. These traits contribute to increased late-stage photosynthate production and dry matter accumulation, supporting a favorable grain-to-leaf ratio for safe and stable grain filling.

      Comparative analysis of metabolite changes during young panicle development in wheat AL type cytoplasmic male serile line and homologous maintainers
      KONG De-Zhen, SANG Wei, NIE Ying-Bin, LI Wei, XU Hong-Jun, LI Jiang-Bo, LIU Peng-Peng, TIAN Xiao-Ming
      Acta Agronomica Sinica. 2025, 51(9):  2454-2466.  doi:10.3724/SP.J.1006.2025.51016
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      To investigate the relationship between the AL-type cytoplasmic male sterile (CMS) wheat line and the metabolome of its maintainer line during spikelet development, and to provide a theoretical foundation for the use of exogenous hormones in regulating fertility restoration, the AL-22A sterile line and the AL-22B homotypic maintainer line were used as experimental materials. Self-seed setting rate was determined using international standard methods. Spike samples were collected at nine developmental stages: young panicle differentiation, double ridge, floret primordium differentiation, pistil and stamen differentiation, chamber formation, tetrad, mononuclear median, uninucleate, binucleate, and trinucleate stages. The levels of endogenous hormones, peroxidase (POD), reactive oxygen species (ROS), and ATP were measured using liquid chromatography-mass spectrometry (LC-MS), and metabolites were analyzed through non-targeted metabolomics. The self-seed setting rate of the sterile line was zero, indicating complete sterility. Compared to the maintainer line, the sterile line showed higher levels of abscisic acid (ABA) and lower levels of indole-3-acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid (GA3). Methyl jasmonate (MeJA) content was lower in the sterile line from the double ridge to tetrad stages but higher after the mononuclear median stage. The IAA/ABA ratio was consistently lower in the sterile line, suggesting that hormone imbalance may contribute to sterility, particularly the elevated levels of ABA and MeJA. No significant differences in ATP and ROS levels were observed between the two lines across developmental stages. However, POD activity was significantly higher in the maintainer line from the double ridge to tetrad stages, while the sterile line showed distinct POD levels at the binucleate and trinucleate stages. Non-targeted metabolomic analysis revealed that the sterile line had more downregulated metabolites during the pistil and stamen differentiation and mononuclear median stages, while other stages showed more upregulated than downregulated metabolites, with significant differences. Clustering analysis of metabolite expression patterns across developmental stages grouped samples into early (I-III), middle (IV-VI), and late (VII-IX) phases. Stage-specific differences in metabolite expression were associated with fertility changes. Pathway enrichment analysis indicated that differential metabolites were primarily concentrated in the floret primordium differentiation, chamber formation, tetrad, uninucleate, and trinucleate stages, mainly involving carboxylic acid metabolism and secondary metabolism. The stages most critical to fertility development occurred in early to mid (II-IV) and mid to late (VI-VIII) phases. These results suggest that the interplay between endogenous hormone balance and abnormal metabolite accumulation may be key factors in CMS. This study provides both theoretical insights and practical references for understanding the physiological and metabolic mechanisms of spikelet development in CMS wheat and for developing strategies to promote fertility restoration in AL-type sterile lines.

      Effects of straw returning and phosphorus application rates on grain starch and the activities of starch synthesis-related enzymes in dryland wheat
      YANG Ying-Cong, ZHANG Jun-Hao, TANG Yi-Zhe, QIAO Chang-Chang, WANG Peng-Bo, HUANG Ming, XU Guo-Wei, WANG He-Zheng
      Acta Agronomica Sinica. 2025, 51(9):  2467-2484.  doi:10.3724/SP.J.1006.2025.51027
      Abstract ( 79 )   HTML ( 3 )   PDF (606KB) ( 20 )   Save
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      To investigate the effects of straw incorporation and phosphorus application rates on starch quality and the synthesis of related enzymes in dryland wheat grains, a split-plot field experiment was conducted using the cultivar Luohan 22. The main plot factor was straw incorporation, with two treatments: no straw return (S0) and full straw incorporation (S1). The sub-plot factor was phosphorus application, with five levels: 0 kg hm-2 (P0), 75.0 kg hm-2 (P1), 112.5 kg hm-2 (P2), 150.0 kg hm-2 (P3), and 187.5 kg hm-2 (P4). The effects of these treatments on wheat yield, starch composition, and the activity of starch-synthesizing enzymes were examined. Results showed that both straw incorporation and increasing phosphorus application enhanced the number of ears, grains per ear, 1000-grain weight, and overall yield. Under the same straw treatment, these yield components generally increased with phosphorus application up to P3, followed by a decline; the highest values (except for 1000-grain weight) were observed at P3. Over the two growing seasons, yield under the S1P3 treatment increased by 35.70% and 49.32% compared with S1P0. The contents of amylose, amylopectin, and total starch in wheat grains increased progressively during grain development and followed a similar rise-then-decline trend with increasing phosphorus, peaking at P3. For any given phosphorus level, the starch content was higher under S1 than S0. In 2021-2022, the interaction between straw incorporation and phosphorus application significantly or highly significantly affected the amylopectin/amylose ratio from 5 to 20 days after anthesis, while in 2022-2023, this interaction was only significant 5 days after anthesis. Throughout both years, the activities of sucrose synthase (SS), ADP-glucose pyrophosphorylase (AGPase), soluble starch synthase (SSS), and granule-bound starch synthase (GBSS) in grains followed a pattern of increasing and then decreasing with grain development, whereas sucrose phosphate synthase (SPS) activity declined continuously. Regardless of straw treatment, AGPase and GBSS activities showed a similar rise-then-decline pattern with phosphorus level, peaking at P3. In conclusion, the combination of straw incorporation and phosphorus fertilization at 150.0 kg hm-2 (S1P3) was the most effective management strategy for improving starch quality and yield in dryland wheat under the conditions of this study.

      Effects of different types and ratios of water-soluble fertilizers on the yield and quality of table-use sweet potato [Ipomoea batatas (L.) Lam.] under drip irrigation
      ZHANG Hai-Yan, XIE Bei-Tao, DONG Shun-Xu, ZHANG Li-Ming, DUAN Wen-Xue
      Acta Agronomica Sinica. 2025, 51(9):  2485-2500.  doi:10.3724/SP.J.1006.2025.54021
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      To investigate the effects of different nitrogen, phosphorus, and potassium (NPK) ratios on the yield and quality of table-use sweet potato (Ipomoea batatas (L.) Lam.) under drip irrigation, field experiments were conducted using two cultivars, Jishu 26 and Yanshu 25, at two sites: Pingyin and Sishui. The study examined the impact of water-soluble fertilizers on storage root yield, starch content, and soluble sugar content. Four treatments were applied: (1) a control (CK) with a basal application of compound fertilizer (N:P2O5:K2O = 15:15:15) at 450 kg hm-2; (2) F1, a drip-applied water-soluble fertilizer (N:P2O5:K2O = 16:6:36) at 150 kg hm-2 applied at 20, 50, and 80 days after transplanting; (3) F2, a drip-applied water-soluble fertilizer (N:P2O5:K2O = 8:12:35) at 150 kg hm-2 applied at the same intervals; and (4) F3, a combination of 150 kg hm-2 of N:P2O5:K2O = 16:6:36 at 20 days after transplanting and 150 kg hm-2 of N:P2O5:K2O = 8:12:35 at 50 and 80 days. The results showed that all water-soluble fertilizer treatments significantly improved yield and marketable root rate compared to the control, with performance ranked as F2 > F3 > F1. For Jishu 26, yields increased by 10.04%, 28.60%, and 19.23% under F1, F2, and F3 at Pingyin, and by 10.88%, 29.55%, and 21.29% at Sishui, respectively. For Yanshu 25, yields increased by 7.62%, 27.79%, and 20.12% at Pingyin, and by 8.52%, 29.17%, and 19.90% at Sishui under the same treatments. In the middle and late stages of growth, F2 significantly reduced vine length, leaf number, and aboveground biomass, while significantly increasing the dry weight of storage roots. Additionally, F2 resulted in significantly lower starch content and significantly higher soluble sugar content compared to the other treatments. Therefore, under the given experimental conditions (soil available nitrogen content ≤ 80 mg kg-1) the F2 treatment—applying 150 kg hm-2 of water-soluble fertilizer (N:P2O5:K2O = 8:12:35, humic acid content ≥ 3%) at 20, 50, and 80 days after transplanting—is recommended as the optimal fertilization strategy for table-use sweet potato production under drip irrigation.

      Effect of replacing common urea with controlled-release fertilizer on dry matter accumulation, partitioning, yield, and quality of sorghum in dryland farming
      FU Jiang-Peng, LIU Fa-Cai, YAN Bao-Qin, WANG Yong-Dong, LI Li-Li, WEI Wei, ZHOU Ying-Xia
      Acta Agronomica Sinica. 2025, 51(9):  2501-2513.  doi:10.3724/SP.J.1006.2025.54002
      Abstract ( 123 )   HTML ( 2 )   PDF (781KB) ( 31 )   Save
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      The substitution of conventional urea with controlled-release fertilizers (CRFs) offers a more efficient fertilization strategy that reduces chemical nitrogen input while enhancing crop yield and quality. A field experiment was conducted from 2022 to 2023 in Gaoping town, Jingchuan county, located on the arid plateau region of the Longdong Loess Plateau, using the sorghum variety Jiza 127. Seven treatments were implemented: no nitrogen application (CK), conventional urea at 240 kg hm-2 of pure nitrogen (CU240), and five CRF-based treatments with varying nitrogen reduction rates—0 (CRU240), 10% (CRU216), 20% (CRU192), 30% (CRU168), and 40% (CRU144). The study aimed to evaluate the effects of replacing conventional urea with CRFs on dry matter accumulation and partitioning, as well as yield and grain quality in sorghum. Results showed that throughout the growth period, the dry matter accumulation rate (DMAR) followed a typical pattern of increasing and then decreasing, peaking during the jointing-to-heading stage. Logistic models fitted to DMA dynamics demonstrated strong goodness-of-fit (R2 = 0.949-0.985) across all treatments. Nitrogen application reduced the proportion of dry matter retained in stems and leaves but significantly enhanced translocation to the panicle at maturity. Notably, the proportion of dry matter allocated to the panicle in CRU240 and CRU216 treatments was, on average, 1.78% and 0.94% higher, respectively, than in CU240. Before flowering, pre-flowering dry matter translocation (DMR), translocation efficiency (DMRE), and the contribution of pre-flowering translocation to grain dry matter accumulation (DMRCG) were significantly lower in CRU168 and CRU144 compared to CU240, while no significant differences were observed in CRU240, CRU216, and CRU192. After flowering, post-flowering DMA in CRU240, CRU216, and CRU192 treatments was comparable to CU240, but DMA in CRU192 was 8.86% and 14.15% higher than that in CRU168 and CRU144, respectively. The highest yield was achieved under CRU240, which was significantly greater than CRU168 and CRU144 by 19.36% and 33.01%, respectively. As nitrogen input decreased, yield declined accordingly, with reductions of 3.63%, 8.83%, 16.20%, and 24.82% observed in CRU216, CRU192, CRU168, and CRU144, respectively, compared to CRU240. Grain protein content peaked in CRU240, being significantly higher than in CRU168 and CRU144 by 19.92% and 20.70%, respectively. In contrast, starch content increased as nitrogen input decreased. Tannin content did not differ significantly among treatments. Path analysis indicated that panicle grain weight and 1000-grain weight were the key determinants of high and stable yields. In conclusion, compared to conventional urea, reducing controlled-release nitrogen application by 10%-20% effectively promotes dry matter accumulation, partitioning, and translocation, while maintaining high yield and improving grain quality. This strategy enhances both agronomic performance and resource use efficiency and is therefore recommended as an optimal nitrogen management approach for dryland sorghum production.

      Characteristics of light distribution in maize‖alfalfa intercropping systems and their effects on plant traits and yield
      YANG Shu, BAI Wei, CAI Qian, DU Gui-Juan
      Acta Agronomica Sinica. 2025, 51(9):  2514-2526.  doi:10.3724/SP.J.1006.2025.53004
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      To elucidate the light resource utilization mechanism in maize‖alfalfa intercropping systems and identify the optimal row configuration for dryland agriculture in Northeast China, a field experiment was conducted in Fuxin, Liaoning—a representative dryland region—during 2021-2022. Five planting patterns were evaluated: intercropping of two rows of maize with two rows of alfalfa (2M2A), two rows of maize with four rows of alfalfa (2M4A), four rows of maize with four rows of alfalfa (4M4A), as well as sole maize (M) and sole alfalfa (A). The effects of intercropping on light distribution, plant traits, and yield were investigated. The results showed that the photosynthetically active radiation (PAR) within the alfalfa canopy was significantly lower than that within the maize canopy. Intercropping improved the light environment of maize: compared with sole maize, light transmittance at the ear layer increased by 28.8%-178.4%, and a similar trend was observed at the canopy base, though differences were not statistically significant. In contrast, the light environment in intercropped alfalfa deteriorated, with canopy light transmittance reduced by 21.4%-59.2% and bottom light transmittance reduced by 40.3%-50.3% compared to sole alfalfa. Intercropping significantly increased stem diameter, leaf area per plant at the filling stage, and ear length of maize, while ear diameter slightly increased and bald tip length slightly decreased. Maize yield in intercropping systems increased by 13.29%-28.22%, mainly due to a significant increase in ear number and grains per ear. However, alfalfa hay yield decreased by 20.91%-49.20% compared to sole alfalfa. In summary, intercropping altered the light distribution within the maize ‖ alfalfa system. An appropriate row configuration can balance light competition between the two crops, improve plant traits, and enhance yield. Among the systems tested, 2M4A showed the highest land equivalent ratio (1.01), indicating a moderate intercropping advantage, and its net economic benefit was 0.8% and 10.5% higher than that of sole maize and sole alfalfa, respectively. Although the land equivalent ratio of 4M4A was 1.00 (no intercropping advantage), its net benefit was still 1.9% and 11.8% higher than that of sole maize and sole alfalfa. Considering both intercropping advantages and economic returns, 2M4A is recommended as a suitable intercropping model for dryland areas in Northeast China.

      RESEARCH NOTES
      Functional verification of the key gene NtLPAT involved in lipid biosynthesis in tobacco
      JI Bai-Lu, SUN Yi-Wen, LIU Wan-Feng, QIAN Ya-Xin, JIANG Cai-Hong, GENG Rui-Mei, LIU Dan, CHENG Li-Rui, YANG Ai-Guo, HUANG Li-Yu, LI Xiao-Xu, PU Wen-Xuan, GAO Jun-Ping, ZHANG Qiang, WEN Liu-Ying
      Acta Agronomica Sinica. 2025, 51(9):  2527-2537.  doi:10.3724/SP.J.1006.2025.44216
      Abstract ( 97 )   HTML ( 3 )   PDF (16722KB) ( 24 )   Save
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      Lysophosphatidyl transferase (LPAT) is a key enzyme in the lipid biosynthesis pathway, catalyzing the transfer of a fatty acyl group from fatty acyl-CoA (Acyl-CoA) to lysophosphatidic acid (LPA) to produce phosphatidic acid (PA). However, the functional role of LPAT in tobacco remains largely unexplored. In this study, we cloned the NtLPAT gene from the tobacco cultivar K326 and generated an NtLPAT knockout mutant (ntlpat) using CRISPR/Cas9 technology. The ntlpat mutant was evaluated for agronomic traits, disease resistance, and phenotypic appearance. In addition, lipidomic and transcriptomic analyses were conducted to assess the impact of NtLPAT loss of function. Our results showed that NtLPAT expression was induced by infection with Ralstonia solanacearum and Phytophthora parasitica. The ntlpat mutant exhibited reduced plant height but enhanced resistance to cucumber mosaic virus (CMV) and bacterial wilt. Lipidomic analysis revealed altered glyceride metabolism in the mutant: triacylglycerol (TAG) levels were significantly decreased, while the contents of two major glycerol glycolipids—monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), both critical components of the thylakoid membrane—were increased. Additionally, changes were observed in sphingolipid and phosphatidylinositol compositions. Transcriptomic analysis indicated that genes involved in photosynthesis, carbon fixation, sphingolipid biosynthesis, and phosphatidylinositol signaling pathways were reprogrammed in ntlpat. These findings suggest that NtLPAT possesses acyltransferase activity, regulates de novo TAG biosynthesis, and plays a role in cellular signaling pathways, thereby affecting tobacco growth and resistance to CMV and R. solanacearum. This study provides valuable genetic resources and an experimental basis for tobacco breeding improvement.

      Screening of low glycemic potato varieties (lines) based on cooking methods and regeneration temperature
      ZHUO Feng-Qi, TANG Zhen-San, LEI Yu-Jun, CHENG Li-Xiang, ZHAO Tian-Tian, LYU Tai, YANG Chen, ZHANG Feng
      Acta Agronomica Sinica. 2025, 51(9):  2538-2546.  doi:10.3724/SP.J.1006.2025.54023
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      This study investigated the effects of different cooking methods and starch retrogradation temperatures on the glycemic index (GI) of potato tubers, aiming to identify cultivars with inherently low GI values that could serve as candidates for nutrition-oriented breeding. Twenty major commercial cultivars and advanced breeding lines were used as experimental materials. Total starch (TS), amylose, dietary fiber, rapidly digestible starch (RDS), slowly digestible starch (SDS), resistant starch (RS), and GI were measured in baked, microwaved, and steamed tubers subjected to retrogradation at 40℃, 30℃, and 20℃. Statistical comparisons and stability analyses were conducted. The results showed that, compared to raw tubers, cooking significantly reduced the contents of TS (by 1.21% FW), RS (8.06% FW), and soluble dietary fiber (1.32% FW), while RDS, SDS, and insoluble dietary fiber increased significantly by 4.75% FW, 3.20% FW, and 5.63% FW, respectively. Among the cooking methods, microwave processing resulted in the lowest GI (69.52). As retrogradation temperature decreased from 40℃ to 20℃, GI and RDS content significantly declined, while RS significantly increased. At 20℃, the lowest GI (72.99) and RDS (5.74% FW) values were observed, along with the highest RS content (7.01% FW). GI was strongly positively correlated with RDS (r = 0.90) and SDS (r = 0.43), and negatively correlated with RS (r = -0.58) and dietary fiber content (r = -0.34). The cultivar Lucinda demonstrated consistently low and stable GI values across all cooking methods and retrogradation temperatures, with a GI of 65.26. RDS and SDS contents after cooking were identified as the main factors influencing GI, and their conversion to RS was dependent on retrogradation temperature. Microwave treatment proved to be the most effective method for reducing the GI in potato tubers. Lucinda was identified as the most promising low-GI cultivar across different cooking methods and retrogradation conditions.

      Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique
      WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie
      Acta Agronomica Sinica. 2025, 51(9):  2547-2556.  doi:10.3724/SP.J.1006.2025.42051
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      Rice stripe disease, transmitted by the brown planthopper, poses a major threat to rice (Oryza sativa L.) production, particularly affecting japonica rice (Oryza sativa subsp. japonica). The causal agent is the rice stripe virus (RSV), which causes significant yield losses. To accelerate the breeding of rice varieties resistant to rice stripe disease, this study aimed to develop functional markers for the rapid and accurate identification of RSV resistance genes, thereby improving the efficiency of rice germplasm enhancement. STV11, a resistance gene identified in the indica variety Kasalath, was targeted. Based on a six-base deletion polymorphism in the Kasalath-type resistance allele STV11KAS (LOC_Os11g30910), sequence data from NCBI were analyzed. A PCR-based functional molecular marker, stvHRM-3, was designed according to nucleotide differences at positions 773-779 between resistant and susceptible varieties. PCR amplification and sequencing of the target fragments were conducted to validate the marker’s specificity. Through HRM-PCR detection and sequencing analysis, stvHRM-3 was confirmed as a functional marker for the STV11 gene. Using this marker, the STV11 genotypes of 520 japonica rice accessions—including materials from the Jiangsu Provincial Late Japonica Rice Regional Trials, late-maturing medium japonica preliminary tests, breeding intermediates, and selected varieties—were analyzed. Results showed that 217 accessions carried the resistance allele, 294 carried the susceptibility allele, and nine exhibited a heterozygous genotype. Accessions identified as resistant through marker analysis consistently exhibited high or moderate levels of resistance. The stvHRM-3 marker, developed using HRM-PCR technology, enables rapid, high-throughput genotyping of STV11 alleles and provides an effective tool for the early screening of RSV resistance. This marker holds great potential for application in marker-assisted selection and breeding of stripe virus-resistant rice varieties.

Co-sponsored:
the Crop Science Society of China
the Institute of Crop Science, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Chen Xiao-ya Yang Jian-chang Zhang Xian-long Wang Jian-kang Xu Ming-liang Liu Chun-ming Wang Dao-wen Sun Chuan-qing Ding Yan-feng Jin Wei-wei Chu Cheng-cai Cheng Wei-hong
Director of the editorial department:
Yan Chun-ling
CN 11-1809/S
ISSN 0496-3490
Post subscription code: 82-336

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  • Started in 2013
  • Covered by SCIE
  • Open access in ScienceDirect

Editor in chief: Wan Jian-min
CN 10-1112/S
ISSN 2095-5421, 2214-5141(online)
Online published:
https://www.sciencedirect.com/journal/the-crop-journal
Submission: https://www.editorialmanager.com/cj/
E-mail: cropjournal@caas.cn

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