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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (12): 2112-2120.doi: 10.3724/SP.J.1006.2008.02112

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Indica-Japonica Characteristics in Thermo-Sensitive Genic Male Sterile Rice Line Zhu 1S

LIU Ping1**,DAI Xiao-Jun1**,YANG Yuan-Zhu2,LI Wen-Jia1,OU Li-Jun1,LIANG Man-Zhong1,CHEN Liang-Bi1*   

  1. 1 College of Life Science, Hunan Normal University, Changsha 410081, Hunan; 2 Academy of Yahua Seeds Sciences, Changsha 410116, Hunan, China
  • Received:2008-03-31 Revised:2008-06-13 Online:2008-12-12 Published:2008-09-06
  • Contact: CHEN Liang-Bi

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Abstract:

Zhu 1S is an early indica thermo-sensitive genic male sterile (TGMS) rice line widely used in hybrid rice production. In this study, with typical indica and japonica cultivars as control, the DNA polymorphism of Zhu 1S was analyzed by using three molecular markers of ISSR, SRAP, and TRAP. The results showed that the nuclear genome of Zhu 1S was mainly indica-derived, but it contained some japonica-specific DNA fragments and had certain japonica consanguinity. The specific molecular fingerprint of Zhu 1S could be established by using any of the three molecular markers. Moreover, the analysis of ORF100, ORF29–TrnCGCA, TrnTUGU–TrnLUAA, intron sequences of rps16 gene in chloroplast DNA of Zhu 1S showed that the chloroplast genome of Zhu 1S was indica-type, while the chloroplast genomes of Pei’ai 64S and Zhun S were japonica-type. There were two base substitutions in TrnTUGU–TrnLUAA fragment of Zhu 1S. It should be applicable approach for breeding two-line early hybrid rice combination with high and stable yield by using dual-purpose genic male sterile lines with indica-type cytoplasm and appropriate japonica consanguinity in double-cropping rice area around the middle and lower reaches of the Yangtze river. Hence, the results have some instructions for the breeding of high quality dual-purpose genic male sterile lines and the rice combinations.

Key words: Thermo-sensitive genic male sterile, Molecular markers, Nuclear DNA, Chloroplast DNA

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