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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 724-732.doi: 10.3724/SP.J.1006.2009.00724

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Photosynthesis and Related Physiological characteristics Affected by Exogenous Glucose in Wheat Seedlings under Water Stress

HU Meng-Yun;Li Hui*;ZHANG Ying-Jun;LIU Qian   

  1. Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and forestry Sciences/Hebei Provincial Laboratory of Crop Genetics and Breeding, Shijiazhuang 050031,China
  • Received:2008-08-28 Revised:2008-10-25 Online:2009-04-12 Published:2009-01-16
  • Contact: LI Hui E-mail:zwslihui@163.com

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Abstract:

Drought, one of the main adverse environmental factors, obviously affects plant growth and development. Previous studies have shown one of the plant mechanisms conferred stress tolerance is the rapid accumulation of soluble sugar (glucose, fructose and sucrose) during water stress. A large number of stress responsive genes have been reported to be induced by glucose, indicating the role of sugars in environmental responses. Although it has already been shown that sugars act as signaling molecules in plants to modulate growth, development, and stress responses, little is known about the mechanisms by which plants respond to them under water stress. To investigate the regulational relation between glucose and drought resistance in wheat, the effects of exogenous glucose on growth and photosynthesis in wheat (Triticum aestivum L.) seedlings under water stress were studied. Wheat seedlings were treated with Hoagland solution (T0), 15% PEG-6000 (T1), 15% PEG-6000+100 μmol L−1 Glc (T2), and 15% PEG-6000+300 μmol L−1Glc (T3), respectively. Effects of exogenous glucose on the leaf water potential and relative water content, photosynthesis, photosynthetic pigmentcontent, soluble sugar and proline content in leaves, as well as growth of seedlings and roots were analyzed. The leaf water potential and leaf photosynthesis decreased significantly, and the growth of seedlings was inhibited when the plants were subjected to water stress. However, the leaf water potential, photosynthetic pigments content increased in T2 and T3 treatments. Simultaneously, the net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), and water use efficiency (WUE) in leaf increased. However, the but transpiration rate (Tr) decreased when the seedlings were treated with exogenous glucose under water stress and rewatered after water stress. Moreover,the levels of proline and soluble sugar in leaves increased by glucose treatment under water stress. Exogenous glucose treatment increased the number of lateral roots, and the length of adventitious roots, and the plant dry matter increased by 14.32–40.39% compared to the drought treatment alone. These results indicate that exogenous glucose may improve drought resistance by increasing roots growth and photosynthesis in leaves under water stress.

Key words: Wheat, Glucose, Water stress, Photosynthesis, Growth

[1] Glombitza S, Dubuis P H, Thulke O, Welzl G, Bovet L, Gotz M, Affenzeller M, Geist B, Hehn A, Asnaghi C, Ernst D, Seidlitz H K, Gundlach H, Mayer K F, Martinoia E, Werck R D, Mauch F, Schaffner A. Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism. Plant Mol Biol, 2004, 51: 1–19
[2] Ashraf M, Foolad M R. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot, 2007, 59: 206–216
[3] Gibson S I. Plant sugar-response pathway. Part of a complex regulatory web. Plant Physiol, 2000, 124: 1532–1539
[4] Couée I, Sulmon C, Gouesbet G, El Amrani A. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot, 2006, 57: 449–459
[5] Furuichi T, Mori IC, Takahashi K, Muto S. Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants. Plant Cell Physiol, 2001, 42: 1149–1155
[6] Ohto M, Onai K, Furukawa Y, Aoki E, Akai T, Nakamura K. Effects of sugar on vegetative development and floral transition in Arabidopsis. Plant Physiol, 2001,127: 252–261
[7] Yuan K, Joanna W D. Phytohormone signalling pathways interact with sugars during seed germination and seedling development. J Exp Bot, 2006, 57: 3359–3367
[8] Shao Y-J(邵艳军), Shan L(山仑), Li G-M(李广敏). Comparison of osmotic regulation and antioxidation between sorghum and maize seedlings under soil drought stress and water recovering conditions. Chin J Eco-agric(中国生态农业学报), 2006, 14(1): 68–70(in Chinese with English abstract)
[9] Praxedes S C, DaMatta F M, Loureiro M E. Effects of long-term soil drought on photosynthesis and carbonhydrate in mature robusta coffee (Coffea canephora Pierre var. kouillou) leaves. Environ Exp Bot, 2006, 56: 263–273
[10] Liu T, van Staden J. Partitioning of carbohydrates in salt-sensitive and salt-tolerant soybean callus cultures under salinity stress and its subsequent relief. Plant Growth Regul, 2001, 33: 13–17
[11] Watanabe S, Kojima K, Ide Y, Sasaki S. Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell, Tissue Organ Culture, 2000, 63: 199–206
[12] Suriyan C, Roytakul S, Sathung T, Maijandang A, Tengsiriwattana S, Kirdmanee C. Exogenous glucose and abscisic acid pre-treatment in indica rice (Oryza sativa L. ssp. indica) responses to sodium chloride salt stress. J Plant Sci, 2007, 2: 141–152
[13] Price J, Laxmi A, Martin S K, Jang J C. Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis. Plant Cell, 2004, 16: 2128–2150
[14] Xu C Y, Jing R L, Mao X G, et al. A wheat (Triticum aestivum) protein phosphatase 2A catalytic subunit gene provides enhanced drought tolerance in tobacco. Ann Bot, 2007, 99: 439–450
[15] Li D-X(李得孝), Hou W-W(侯万伟), Yuan H-Y(员海燕). Fast-soaking methods of chlorophyll from maize leaf. J Northwest A&F Univ (Nat Sci Edn)(西北农林科技大学学报·自然科学版), 2006, 34(11): 65–67 (in Chinese with English abstract)
[16] Zhao S-J(赵世杰), Xu C-C(许长成), Zou Q(邹琦), Meng Q-W(孟庆伟). The improvement of MDA measurement in plant. Plant Physiol Commun (植物生理学通讯), 1994, 30(3): 207–210(in Chinese)
[17] Bates L S, Waldren R P, Teare I D. Rapid determination of free proline for water-stress studies. Plant Soil, 1973, 39: 205?207
[18] Levitt J. Responses of plants to environmental stress. In: Water Relation, Salt and Other Stresses, Vol. 2. 2nd edn. New York: Academic Press, 1980. p 606
[19] Qu Y-Y(曲延英), Mu P(穆平), Li X-Q(李雪琴), Tian Y-X(田玉秀), Wen F(文峰), Zhang H-L(张洪亮), Li Z-C(李自超). QTL mapping and correlations between leaf water potential and drought resistance in rice under upland and lowland environments. Acta Agron Sin (作物学报), 2008, 34(2): 198?206(in Chinese with English abstract)
[20] Gao S-B(高世斌), Feng Z-L(冯质雷), Li W-C(李晚忱), Rong T-Z(荣廷昭). Mapping QTLs for root and yield under drought stress in maize. Acta Agron Sin (作物学报), 2005, 31(6): 718?722(in Chinese with English abstract)
[21] Shao H B, Liang Z S, Shao M A. Changes of some physiological and biochemical indices for soil water deficits among 10 wheat genotypes at seedling stage. Colloids Surface B: Biointerface, 2005, 42: 103–107
[22] Lu P, Outlaw W H, Smith B G, Freed G. A new mechanism for the regulation of stomatal aperture size in intact leaves: Accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba. Plant Physiol, 1997, 114: 109–118
[23] Zhou L-N(周莉娜), Qu D(曲东), Shao L-L(邵丽丽), Yi W-J(易维洁). Effects of sulfur fertilization on the contents of photosynthetic pigments and MDA under drought stress. Acta Bot Boreali-Occident Sin(西北植物学报), 2005, 25(8): 1579–1583(in Chinese with English abstract)
[24] Zou C-J(邹春静), Han S-J(韩士杰), Xu W-D(徐文铎), Li D-T(李道棠). Eco-physiological responses of Picea mongolica ecotypes to drought stress. Chin J Appl Ecol (应用生态学报), 2003, 14(9): 1446–1450(in Chinese with English abstract)
[25] Guo S-K(郭书奎), Zhao K-F(赵可夫). The possible mechanisms of NaCl inhibit photosynthesis of maize seedlings. Acta Phytophysiol Sin (植物生理学报), 2001, 27(6): 461–466 (in Chinese with English abstract)
[26] Hou C-X(侯彩霞), Tang Z-C(汤章城). Function and mechanism of cornpatible solutes. Plant Physiol Commun (植物生理学通讯), 1999, 35(1): 1–7 (in Chinese)
[27] Cheng W H, Endo A, Zhou L, Penney J, Chen H C, Arroyo A, Leon P, Nambara E, Asami T, Seo M, Koshiba T, Sheen J. A unique short-chain dehydrogenase/ reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell, 2002, 14: 2723–2743
[28] Hellmann H, Funck D, Rentsch D, Frommer W B. Hypersensitivit of an Arabidopsis sugar signaling mutant toward exogenous proline application. Plant Physiol, 2000, 123: 779–789
[29] Boriboonkaset T, Bunyakijjinda V, Chaum S, Kirdmanee C. Effect of exogenous sugar classes and concentrations on salt-tolerant ability of indica rice (Oryza sativa L.). Acta Hort (ISHS), 2007, 764: 155–164
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