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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2008-2014.doi: 10.3724/SP.J.1006.2009.02008

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Profile Analysis of Ghpg2 Gene Associated with Fertility in Cotton（Gossypium hirsutum L.）

HOU Si-Yu,ZHANG Rui,GUO San-Dui*   

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081
  • Received:2009-01-08 Revised:2009-05-30 Online:2009-11-12 Published:2009-08-07
  • Contact: GUO San-Dui, Email: gsdui@mail.caas.net.cn

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Abstract:

Polygalacturonase (PGs) gene family is a kind of proteolytic enzyme in cell wall. A number of higher plants have a higher level of the excision activity at pollen. Polygalacturonase plays an important role on the processes of pollen mature and pollen tube growth. Through BAC library screening, a BAC clone Z55E7 was obtained and congregated with molecular marker STS679 by using PCR strategy. Sequencing analysis results showed that there were six genes in the clone. One of them was a Polygalacturonase gene in cotton, named GhPG2. The expression profiles analysis of this gene would help to know the relationshipof GhPG2 gene with the molecular mechanism of cotton cytoplasm male sterile. GhPG2 gene was isolated from BAC clone. The amino acids and nucleotide sequence of the gene were analyzed by bioinformation software. GhPG2 gene expression levels of different tissues in CMS line, maintainer and restorer line were analyzed by RT-PCR and real-time quantitative PCR method. The gene with the sequence of 1518 bp and an open reading frame of 1239bp coded 412 amino acids and included four conserved domains. Multiple alignment and phylogenetic analysis based on the amino acid sequences showed that GhPG2 was categorized as a gene expressed in pollen. GhPG2 gene expression level in anther, petal and the sixth grade flower bud was higher in the maintainer and restorer lines and lower obviously in the CMS line. And its relative transcript levels were 1.73, 1.45; 3.7, 3.4; 1.63, and 1.39 folds compared with petal, anther and VI flower bud of sterile line, respectively.The gene expression level in anther, petal and the sixth grade flower bud was no significance between the maintainer and restorer line with relative transcript levels were 4.33, 4.08; 13.29, 12.12; 3.25 and 3.10 folds, respectively. It is speculated that GhPG2 gene is of significance in floral organ development in cotton.

Key words: Cotton, GhPG2, Flower Development, Polygalacturonase, Expression profiling

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