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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 721-727.doi: 10.3724/SP.J.1006.2010.00721

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Tagging of a Stripe Rust Resistance Gene in a Triticum durumAegilops squarrosa Synthetic Wheat CI191

REN Qiang1,2,LIU Hui-Juan1,**,CHEN Yang1,XU Shi-Chang3,HE Ming-Zhao1,XIN Zhi-Yong1,ZHANG Zeng-Yan1*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:2009-11-24 Revised:2010-02-07 Online:2010-05-12 Published:2010-03-15
  • Contact: ZHANG Zheng-Yan,E-mail: zhangzy@mail.caas.net.cn; Tel: 010-82108781

Abstract:

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most serious fungal diseases in wheat worldwide. To diversify the resistance resources, a resistance synthetic wheat CI191 (CPI/GEDIZ/3/GOO//JO69/CRA/ 4/AE.SQ629) has been mined from 94 accessions of Triticum durum-Aegilops squarrosa synthetic wheat introduced from CYMMT. CI191 show highly resistant to six Pst races prevailing in Chinese, such as CY28, CY29, CY30, CY31, CY32, and CY-Su11-4. Based on the responses of CI191 nd 24 wheat materials possessing known stripe rust resistance genes to 22 Pst races, we postulated CI191 was different from all the known resistant genes. According to analysis of inherited mode, the resistance gene in CI191 was controlled by a single dominate gene, tentatively designated YrC191. Using the cross between CI191 and Mingxian 169 (a sensitive wheat cultivar to Pst races), the F2 segregation population and the bulked segregant pools were established to screen the the simple sequence repeat (SSR) markers linked to YrC191. Seven SSR markers were found to be linked with YrC191 in the alignment of Xwmc419-YrC191/ Xbarc240-Xbarc8-Xcfd65/Xbarc187/Xgwm18/Xgwm11. YrC191 was cosegrated withthe marker Xbarc240, flanked by the closest linked markers Xbarc8 and Xwmc419 with the relative genetic distances of 1.6 cM and 3.1 cM, respectively. Using Chinese Spring nullisomic-tetrasomics and ditelosomic lines of homoeologous group 1, Xcfd65, Xgwm18, Xgwm11, Xbarc187, and Xwmc419 markers were physically mapped on the chromosome arm 1BS, and YrC191 was also located on 1BS. Four SSR markers linked tightly with YrC191 and 1 sequence tagged site (STS) marker for Yr26 could discriminate YrC191 from the other resistant genes on 1BS, such as Yr24, Yr26, Yr10, Yr15, YrCH42, and YrC142. The resusltes suggested that YrC191 may be a novel resistance gene to Pst.

Key words: Triticum durum/Aegilops tauschii synthetic wheat, stripe rust, resistance gene, gene postulation, SSR marker

[1] Xie H(谢皓), Niu Y-C(牛永春), Chen X(陈孝), Ma Y-Z(马有志), Wu L-R(吴立人), Lin Z-S(林志珊). Assessment and genetic analysis on the resistance of a new wheat line YW243 to stripe rust. Acta Phytopathol Sin (植物病理学报), 2003, 33(3): 243–247 (in Chinese with English abstract)

[3] Fang T L(房体麟), Cheng Y(程颖), Li G-Q(李根桥), Xu S-C(徐世昌), Xie C-J(解超杰), You M-S(尤明山), Yang Z-M(杨作民), Sun Q-X(孙其信), Liu Z-Y(刘志勇).Molecular characterization of a stripe rust resistance gene from wheat line S2199 and its allelism with Yr5[J].Acta Agron Sin(作物学报.2008, 34(3):355-

[4] McIntosh R A, Yamazaki Y, Devos K M, Dubcovsky J, Rogers W J, Appels R. Catalogue of gene symbols for wheat. In: Pogna N E, Romano N, Pogna E A, Galterio G, eds. Proceedings of the Tenth International Wheat Genetics Symposium. Vol. 4. Rome: Istituto Sperimentale per la Cerealcoltura, 2003. p 8

[5] McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Anderson O D. Catalogue of gene symbols for wheat: 2007 supplement. http://www.wheat.pw.usda.gov/ggpages/macgene/supplement2007.htm

[6] Zhang H(张宏), Ren Z-L(任志龙), Hu Y-G(胡银岗), Wang C-Y(王长有), Ji W-Q(吉万全). Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139[J].Acta Agron Sin (作物学报.2010, 36:109-114

[7] Mujeeb-Kazi A, Rosas V, Roland S. Conservation of the genetic variation of Triticum Tauschii (Coss[J].) Schmalh. (Aegilops aquarrosa L.) in synthetic hexaploid wheats (T.turgidum L. × T. tauschii, 2n = 6x = 42, AABBDD) and its potential utilization for wheat improvement. Genet Reours Crop Evol

[8] Multani D S, Daliwal H S, Singh P, Gill K S. Synthetic amphiploids of wheat as a source of resistance to Karnal bunt (Neovossia indica). Plant Breed, 100: 122-125

[9] Mujeeb-Kazi A, Fuentes-Davila G, Villareal R L, Cortes A, Rosas V, Delgado R. Registration of 10 synthetic hexaploid wheat and six bread wheat germplasms resistant to Karnal bunt. Crop Sci, 41: 1652-1653

[10] Singh R P, Mujeeb-Kazi A, Huerta-Espino J. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat[J].Phytopathology

[11] Ma H, Singh R P, Mujeeb-Kazi A. Resistance to stripe rust in Triticum turgidum, T. tauschii and their synthetic hexaploids. Euphytica, 1995, 82: 117-124

[12] Ma H, Singh R P, Mujeeb-Kazi A. Resistance to stripe rust in durum wheats, A-genome diploids and their amphiloids[J].Euphytica

[13] Li G Q, Li Z F, Yang W Y, Zhang Y, He Z H, Xu S C, Singh R P, Qu Y Y, Xia X C. Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26[J].Theor Appl Genet

[14] Wang L M, Zhang Z Y, Liu H J, Xu S C, He M Z, Liu H X, Veisz O, Xin Z Y. Identification, gene postulation and molecular tagging of a stripe rust resistance gene in synthetic wheat CI142[J].Cereal Res Commun

[15] Li T(李韬), Zhang Z-Y(张增艳), Lin Z-S(林志珊), Chen X(陈孝), Gao S(高珊), Xin Z-Y(辛志勇). Mapping of a novel gene conferring resistance to wheat powdery mildew using AFLP and SSR markers. Acta Agron Sin (作物学报), 2005, 31: 1105-1109 (in Chinese with English abstract)

[16] Shao Y T, Niu Y C, Zhu L H, Zhai W X, Xu S C, Wu LR. Identification of an AFLP marker linked to the stripe rust resistance gene Yr10 in wheat[J].Chin Sci Bull

[17] Wang L F, Ma J X, Zhou R H, Wang X M, Jia J Z. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, P.I. 178383 (Triticum aestivum L.). Euphytica, 2002, 124: 71-73

[18] Wen W E, Li G Q, He Z H, Yang W Y, Xu M L, Xia X C. Development of an STS marker tightly linked to Yr26 against wheat stripe rust using the resistance gene-analog polymorphism (RGAP) technique. Mol Breed, 2008 22: 507-515

[19] Niu Y-C(牛永春), Qiao Q(乔奇), Wu L-R(吴立人). Postulation of resistance genes to stripe rust in commercial wheat cultivars from Henan, Shandong and Anhui provinces. Phytopathol Sin (植物病理学报), 2000, 30(2): 122-128 (in Chinese with English abstract)

[20] Sharp P G, Kreis M, Shewry P R, Gale M D. Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet, 1988, 75: 289-290

[21] Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007-2023

[22] Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L[J].). Theor Appl Genet

[23] Lincoln S, Daly M, Lander E. Constructing Genetic Maps with Mapmaker/EXP3.0. Whitehead Institute Techn Rep, 3rd Edn. Whitehead Institute, Cambridge, Masachussetts, USA. 1992

[24] Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1944, 12: 172-175

[25] He M-Z(何名召), Wang L-M(王丽敏), Zhang Z-Y(张增艳), Xu S-C(徐世昌), Xin Z-Y(辛志勇). Identification and molecular mapping of a novel stripe rust resistancegene in a Triticum durum-Aegilops tauschii Amphiloid CI108. Acta Agron Sin(作物学报), 33: 1045-1050 (in Chinese with English abstract)

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