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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 905-910.doi: 10.3724/SP.J.1006.2010.00905

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Analysis of MADS-box Gene from Soybean (Glycine max L. Merr.) Cytoplasmic Male Sterile Line

 HAN Li-Chao, JIANG Wei, YANG Shou-Ping, YU De-Ti, GAI Jun-Yi   

  1. National Center for Soybean Improvement,National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2009-12-21 Revised:2010-03-19 Online:2010-06-12 Published:2010-04-14
  • Contact: YANG Shuo-Ping,E-mail:spyang@njau.edu.cn,Tel:025-84396463;GAI Jun-Yi,E-mail:sri@njau.edu.cn, Tel: 025-84395405
  • About author:hanlitao0210@yahoo.com.cn

Abstract:

CMS (cytoplasmic male sterility) plays an important role in the utilization of crop heterosis. It is of important significance on theory and practice to study the genetic base and mechanism of CMS. To reveal the molecular mechanism of soybean cytoplasmic male sterility, we analyzed the gene differential expression between the soybean cytoplasmic male sterile line NJCMS2A and its maintainer line NJCMS2B by the cDNA-AFLP differential display method. A differentially expressed fragment from the flower buds of NJCMS2A was cloned and sequenced. The Blast results showed that the differentially expressed fragment shared 98.7% homology with g29510.1 cDNA fragment on Gm13 of soybean genome, and 98% homology with a MADS-boxgene of soybean. The Blast results of the amino acid sequences indicated that the differentially expressed fragment shared 96% homology with a MADS-box protein of Glycine max, 83% homology with the MADS-box M7 protein of Pisum sativum, 88% homology with the MADS-box 2 protein of Momordica charantia, and 83% homology with AGAMOUS protein of Gossypium barbadense. The structure and function prediction of the amino acid sequence showed that the protein encoded by the differentially expressed fragment was the transcription factor of the MADS-box gene which contained a typical K-box domain. The results of semi-quantitative RT-PCR showed that the expression quantity of the differentially expressed fragment in the flower buds of NJCMS2A was much higher than that of NJCMS2B. According to the above results, it was inferred that the differentially expressed fragment was probably related to the soybean cytoplasmic male sterility.

Key words: Soybean, Cytoplasmic male sterility, cDNA-AFLP, MADS-box transcription factor

[1] Bachem C W B, Van Der Hoeven R S, De Bruijn S M, Vreugdenhil D, Zabeau M, Visser R G F. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development. Plant J, 1996, 9: 745-753

[2] Habu Y, Fukada-Tanaka S, Hisatomi Y, Iida S. Amplified restriction fragment length polymorphism-based mRNA fingerprinting using a single restriction enzyme that recognizes a 4-bp sequence. Biochem Biophys Res Commun,1997, 234: 516-521

[3] Van Der Biezen E A, Juwana H, Parker J E, Jones J D G. cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana. Mol Plant-Microbe Interac, 2000, 13: 895-898


 

[4] Qin L, Overmars H, Helder J, Popeijus H, Van Der Voort J R, Groenink W, Van Koert P, Schots A, Bakker J, Smant G. An efficient cDNA-AFLP-based strategy for the identification of putative pathogenicity factors from the potato cyst nematode Globodera rostochiensis. Mol Plant-Microbe Interac,2000, 13: 830-836

[5] Ling X-Y凌杏元), Zhou P-J周培疆), Huang Q-Y黄青阳), Guan H-X关和新), Zhu Y-G朱英国). Isolation and sequence analysis of a mitochondrial DNA fragment associated with CMS in Hong Lian type rice. Acta Biol Exp Sin (实验生物学报), 2000, 33(2): 151-155 (in Chinese with English abstract)(((((

[6] Wu M-S(吴敏生), Gao Z-H(高志环), Dai J-R(戴景瑞). Studies on differential gene expression of maize (Zea mays L.) by means of cDNA- AFLP technique. Acta Agron Sin (作物学报), 2001, 27(3): 339-342 (in Chinese with English abstract)

[7] Wang Y-Q(王永勤), Cao J-S(曹家树), Fu Q-G(符庆功), Yu X-L(余小林), Ye W-Z(叶纨芝), Xiang X(向珣). Differential expression analysis of genic male sterility A/B lines by cDNA-AFLP in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino). Sci Agric Sin (中国农业科学), 2003, 36(5): 557-560 (in Chinese with English abstract)

[8] Lü S-H(吕山花), Meng Z(孟征). Gene duplication and functional diversification in the MADS-box gene family. Chin Bull Bot (植物学通报), 2007, 24(1): 60-70 (in Chinese with English abstract)

[9] Parenicova L, De Folter S, Kieffer M, Horner D S, Favalli C, Busscher J, Cook H E, Ingram R M, Kater M M, Davies B, Angenent G C, Colombo L. Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: New openings to the MADS world. Plant Cell, 2003, 15: 1538-1551

[10] Nam J, Kim J, Lee S, An G, Ma H, Nei M. Type I MADS-box genes have experienced faster birth-and-death evolution than type II MADS-box genes in angiosperms. Proc Nat Acad Sci USA, 2004, 101: 1910-1915

[11] Michaels S D, Ditta G, Gustafson-Brown C, Pelaz S, Yanofsky M, Amasino R M. AGL24 acts as a promoter of flowering in Arabidopsis and is positively regulated by vernalization. Plant J, 2003, 33: 867-874

[12] Battaglia R, Brambilla V, Colombo L, Stuitje A R, Kater M M. Functional analysis of MADS-box genes controlling ovule development in Arabidopsis using the ethanol-inducible alc gene-expression system. Mech Dev, 2006, 123: 267-276

[13] Wang X-L(汪潇琳), Chen Y-P(陈艳萍), Yu D-Y(喻德跃). Expression of the MADS-box gene GmAGL15 in seed development of soybean. Acta Agron Sin (作物学报), 2008, 34(2): 330-332 (in Chinese with English abstract)

[14] Alvarez-Buylla E R, Liljegren S J, Pelaz S, Gold S E, Burgeff C, Ditta G S, Vergara-Silva F, Yanofsky M F. MADS-box gene evolution beyond flowers: Expression in pollen, endosperm, guard cells, roots and trichomes. Plant J, 2000, 24: 457-466

[15] Bai Y N, Gai J Y. Development of a new cytoplasmic-nuclear male-sterility line of soybean and inheritance of its male-fertility restorability. Plant Breed, 2006, 125: 85-88

[16] Bachem C W B, Oomen R J F J, Visser R G F. Transcript imaging with cDNA-AFLP: A step-by-step protocol. Plant Mol Biol Rep, 1998, 16: 157-173

[17] Coen E S, Meyerowitz E M. The war of the whorls: Genetic interactions controlling flower development. Nature, 1991, 353: 31-37

[18] Ferrario S, Immink R G H, Shchennikova A, Busscher-Lange J, Angenent G C. The MADS box gene FBP2 is required for SEPALLATA function in petunia. Plant Cell, 2003, 15: 914-925

[19] TheiBen G, Saedler H. Floral quartets. Nature, 2001, 409: 469-471

[20] Hu R-B(胡瑞波), Fan C-M(范成明), Li H-Y(李宏宇), Lin C-T(林辰涛), Fu Y-F(傅永福). Analysis of MIKC-type MADS-box genes in soybean (Glycine max). Mol Plant Breed (分子植物育种), 2009, 7(3): 429-436 (in Chinese with English abstract)

[21] Bowman J L, Smyth D R, Meyerowitz E M. Genes directing flower development in Arabidopsis. Plant Cell,1989, 1: 37-52

[22] Murai K, Takumi S, Koga H, Ogihara Y. Pistillody, homeotic transformation of stamens into pistil-like structures, caused by nuclear- cytoplasm interaction in wheat. Plant J, 2002, 29: 169-181

[23] Meguro A, Takumi S, Ogihara Y, Murai K. WAG, a wheat AGAMOUS homolog, is associated with development of pistil-like stamens in alloplasmic wheats. Sex Plant Reprod, 2003, 15: 221-230

[24] Hama E, Takumi S, Ogihara Y, Murai K. Pistillody is caused by alterations to the class-B MADS-box gene expression pattern in alloplasmic wheats. Planta, 2004, 218: 712-720

[25] Sun Q-P(孙清萍), Wang L(汪莉), Yi P(易平), Zhu Y-G(朱英国). Expression analysis of MADS-box gene family on uni-nucleate and bi-nucleate stage anthers on HL-CMS system. Wuhan Bot Res (武汉植物学研究), 2002, 20(5): 325-328 (in Chinese with English abstract)

[26] Yuan Z-Q(袁自强), Qian X-Y(钱晓茵), Liu J(刘军), Liu J-D(刘建东), Qian M(钱旻), Yang J-S(杨金水). cDNA cloning and analysis of two MADS-box genes in rice. Prog Nat Sci (自然科学进展), 2000, 10(2): 129-134 (in Chinese)

[27] Zhou L-L(周琳璘), Song G-Q(宋国琦), Li H-Y(李红燕), Hu Y-G(胡银岗), He B-R(何蓓如). A MADS-box transcription factor related to fertility conversion in male sterile wheat lines. Acta Agron Sin (作物学报), 2008, 34(4): 598-604 (in Chinese with English abstract)
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