冬小麦种质矮孟牛第一部分同源群染色体遗传差异分析

doi:10.3724/SP.J.1006.2010.01450

Abstract

Abstract: Aimengniu (Hereafter AMN), cultivated by Shandong Agricultural University, is a renowned Chinese winter wheat germplasm. Knowing better its genetic component is of great value and necessity for its in-depth utilization and study. Purpose of this study is to reveal the genetic differences in detail among seven AMN-derived types. Both molecular markers and genomic in situ hybridization were used to identify translocation between 1B and 1R in seven AMN-derived types. PCR results of primers specific for 1R and 1B detected the presence of both 1RS and 1BL chromatin and absence of 1BS and 1RL in AMNII and AMNIV to VII, while AMNI and AMNIII contained common 1B chromatin. Genomic in situ hybridization confirmed the replacement of chromosome arm 1BS by 1RS in AMNII and AMNIV to VII, and a typical common wheat karyotype was contained in AMNI and AMNIII. In addition, genetic differences among seven AMN-derived types in homoeologous group 1 were detected by 138 polymorphic markers, and genotypic information for each and every one of seven AMN-derived types were represented. Specific segments of AMNV were detected by 3 markers, Xwmc336-Xmag1884 (1A)and Xgwm124 (1B), originating from Neuzucht and Aifeng3 respectively. The results above revealed that genetic differences exist among seven sister lines of germplasm AMN in homoeologous group 1, which will facilitate its further utilization and study.

Key words: Winter wheat germplasm, Genetic difference, Genomic insitu hybridization, Molecular marker

CUI Fa,Chun-Hua, BAO Yi-Guang, ZONG Hao, WANG Yu-Hai, WANG Qiang-Zhuan, DU Bin, MA Hang-Yuan, WANG Hong-Gang. Genetic Differences in Homoeologous Group 1 of Seven Types of Winter Wheat Aimengniu[J].Acta Agron Sin, 2010, 36(09): 1450-1456.

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Genetic Differences in Homoeologous Group 1 of Seven Types of Winter Wheat Aimengniu

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