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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1752-1759.doi: 10.3724/SP.J.1006.2010.01752

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Mechanisms of Enhanced Uptake of Potassium Nutrition in EdHP1 (H+-pyrophosphatase) Transgenic Tobacco (Nicotiana tabacum)

JIN  Wei-Huan12,DONG  Jian-Hui2,CHEN  Ming2*,CHEN  Yao-Feng1,LI  Lian-Cheng2,XU  Zhao-Shi2,MA  You-Zhi2*   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-03-10 Revised:2010-05-23 Online:2010-10-12 Published:2010-05-23
  • Contact: CHEN Ming,E-mail:chenming@mail.caas.net.cn;MA You-Zhi,E-mail:mayouzhi@yahoo.com.cn

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Abstract: Potassium is necessary for plant growth and enhancing uptake of potassium is important to improve crop yield. In this study, previous transgenic tobacco transformed with Elymus dahuricus EdHP1 (H+-pyrophosphatase) was used to compare the physiological characteristics between transgenic tobacco and wild type tobacco. The results showed that under starvation of potassium, the content of K+ accumulated in wild-type tobacco was 62.9% of that in transgenic tobacco, and the total root length, total root surface area, and total root volume in wild type tobacco were observably lower than those in transgenic tobacco (P<0.05), with the decrease of 72.9%, 68.1%, and 60.6%, respectively. In addition, in different root systems of fine roots (diameter=0.5–2.0 mm), middle roots (diameter= 2.5–4.0 mm) and coarse roots (diameter>4.5 mm), fine roots were biggest different between transgenic and wild type tobacco which fine roots were 72.9% of those in transgenic tobacco. The activity of root system in transgenic tobacco was 1.2 times as high as that in wild type tobacco. The activity of H+-pyrophosphatase and the content of free IAA in wide-type tobacco were only 46.4% and 66.2% of those in transgenic tobacco respectively. The results of non-destructive ion flux measurement showed that under starvation of potassium the efflux of K+ from wild type tobacco was observably higher than that from transgenic tobacco, but inverse result was found efflux of H+ with similarly total root surface area of transgenic tobacco and wild type tobacco. Two physiological mechanisms containing well-developed root system and higher activity of K+ transporters enhanced uptake of potassium in EdHP1 transgenic tobacco plants.

Key words: Tobacco, Uptake of potassium, H+-pyrophosphatase, Root system

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