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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1505-1510.doi: 10.3724/SP.J.1006.2011.01505

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Genetic Stability of Rice Aneuploid during Its Asexual Propagation

GONG Zhi-Yun,SHI Guo-Xin,LIU Xiu-Xiu,YI Chuan-Deng,YU Heng-Xiu*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2011-01-12 Revised:2011-05-20 Online:2011-09-12 Published:2011-06-28
  • Contact: 于恒秀, E-mail: hxyu@yzu.edu.cn, ?Tel: 0514-87979304, Fax: 0514-87972138

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Abstract: Telotetrasome is a kind of aneuploid with two additional identical telocentric chromosomes. To investigate the genetic stability of riceaneuploid during its asexual propagation,a telotetrasome (2n+·8S+·8S) was selected from the progenies of a rice telotrisome (2n+·8S), and preserved by asexsual reproduction. But one of the extra short arms (·8S) was easy to be lost in the asexual propagation offspring of 2n+·8S+·8S and led to morphological variations. FISH results indicated that one of the two extra ·8S was short of detectable rice centromeric satellite repeat (CentO) and centromere-specific retrotransposon (CRR) and could not be transmitted stably. The other extra ·8S contained CRR, but not detectable CentO and could be transmitted steadily. However, the extra ·8S contained the CentO and CRR sequences simultaneously in the initial telotrisomic line (2n+·8S). These results showed that CentO and CRR of the extra ·8S may be randomly lost and led inheritance instability in the aneuploid harbouring extra ·8S during asexual propagation.

Key words: Rice, Aneuploid, Telocentric chromosome, CentO, CRR

[1]Yu W, Han F, Kato A, Birchler J A. Characterization of a maize isochromosome 8S*8S. Genome, 2006, 49: 700–706
[2]Cheng Z K, Yan H H, Dang B Y. Microdissection and amplification of the chromosome arm 5S in a rice telo-tetrasomic. Chin Sci Bull, 1998, 43: 590–594 
[3]Wang Z X, Ideta O, Yoshimura A, Iwata N. Identification of extra chromosome of aneuhaploids and tetrasomics in rice and the use of these aneuhaploids in genome analysis. Breed Sci, 1995, 45: 327–330
[4]Cheng Z K, Yan H H, Yu H X, Tang S C, Jiang J M, Gu M H, Zhu L H. Development and applications of a complete set of rice telotrisomics. Genetics, 2001, 157: 361–368
[5]Cheng Z-K(程祝宽), Yan H-H(颜辉煌), Yu H-X(于恒秀), Qian Q(前钱), Yi C-D(裔传灯), Gu M-H(顾铭洪), Zhu L-H(朱立煌). Fast assignment of DNA sequences to individual chromosome arms based on dosage effects from a set of rice telotrisomics. Acta Bot Sin (植物学报), 2000, 42(7): 708–711 (in Chinese with English abstract)
[6]Houben A, Schubert I. DNA and proteins of plant centromeres. Curr Opin Plant Biol, 2003, 6: 554–560
[7]Malik H S, Henikoff S. Conflict begets complexity: the evolution of centromeres. Curr Opin Genet Dev, 2002, 12: 711–718
[8]Nagaki K, Kashihara K, Murata M. A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco. Chromosoma, 2009, 118: 249–257
[9]Cheeseman I M, Drubin D G, Barnes G. Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast. J Cell Biol, 2002, 157: 199–203
[10]Clarke L, Carbon J. Genomic substitutions of centromeres in Saccharomyces cerevisiae. Nature, 1983, 305: 23–28
[11]Clarke L. Centromeres of budding and fission yeasts. Trends Genet, 1990, 6: 150–154
[12]Ananiev E V, Phillips R L, Rines H W. Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions. Proc Natl Acad Sci USA, 1998, 95: 13073–13078
[13]Kamm A, Galasso I, Schmidt T, Heslop-Harrison J S. Analysis of a repetitive DNA family from Arabidopsis arenosa and relationships between Arabidopsis species. Plant Mol Biol, 1995, 27: 853–862
[14]Sun X, Wahlstrom J, Karpen G. Molecular structure of a functional Drosophila centromere. Cell, 1997, 91: 1007–1019
[15]Schueler M G, Higgins A W, Rudd M K, Gustashaw K, Willard H F. Genomic and genetic definition of a functional human centromere. Science, 2001, 294: 109–115
[16]Wevrick R, Willard H F. Long-range organization of tandem arrays of alpha satellite DNA at the centromeres of human chromosomes: high-frequency array-length polymorphism and meiotic stability. Proc Natl Acad Sci USA, 1989, 86: 9394–9398
[17]Ma J, Jackson S A. Retrotransposon accumulation and satellite amplification mediated by segmental duplication facilitate centromere expansion in rice. Genome Res, 2006, 16: 251–259
[18]Henikoff S, Ahmad K, Malik H S. The centromere paradox: stable inheritance with rapidly evolving DNA. Science, 2001, 293: 1098–1102
[19]Hosouchi T, Kumekawa N, Tsuruoka H, Kotani H. Physical map-based sizes of the centromeric regions of Arabidopsis thaliana chromosomes 1, 2, and 3. DNA Res, 2002, 9: 117–121
[20]She C-W(佘朝文), Song Y-C(宋运淳). Advances in research of the structure and function of plant centromeres. Hereditas(遗传), 2006, 28(12): 1597–1606 (in Chinese with English abstract)
[21]Cheng Z, Dong F, Langdon T, Ouyang S, Buell C R, Gu M, Blattner F R, Jiang J. Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell, 2002, 14: 1691–1704
[22]Nagaki K, Cheng Z, Ouyang S, Talbert P B, Kim M, Jones K M, Henikoff S, Buell C R, Jiang J. Sequencing of a rice centromere uncovers active genes. Nat Genet, 2004, 36: 138–145
[23]Kurata N. OT. Karyotype analysis in rice I. A new method for identifying all chromosome pairs. Jpn J Genet, 1978, 53: 251–255
[24]Wu H K. Note on preparing of pachytene chromosomes by double mordant. Sci Agric, 1967, 15: 40–44
[25]Jiang J, Gill B S, Wang G L, Ronald P C, Ward D C. Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes. Proc Natl Acad Sci USA, 1995, 92: 4487–4491
[26]Cheng Z, Buell C R, Wing R A, Jiang J. Resolution of fluorescence in-situ hybridization mapping on rice mitotic prometaphase chromosomes, meiotic pachytene chromosomes and extended DNA fibers. Chromosome Res, 2002, 10: 379–387
[27]Diao X-M(刁现民), Sun J-S(孙敬三). Cytological and molecular biological research progress in plant somaclonal variation. Chin Bull Bot (植物学通报), 1999, 16(4): 372–377 (in Chinese with English abstract)
[28]Larkin P J, Scowcroft W P. Somaclonal variation novel source of variability from cell culture for plant improvement. Theor Appl Genet, 1981, 60: 197–214
[29]Thomas J W, Schueler M G, Summers T J, Blakesley R W, McDowell J C, Thomas P J, Idol J R, Maduro V V, Lee-Lin S Q, Touchman J W, Bouffard G G, Beckstrom-Sternberg S M, Green E D. Pericentromeric duplications in the laboratory mouse. Genome Res, 2003, 13: 55–63
[30]Maggert K A, Karpen G H. The activation of a neocentromere in Drosophila requires proximity to an endogenous centromere. Genetics, 2001, 158: 1615–1628
[31]Amor D J, Choo K H. Neocentromeres: role in human disease, evolution, and centromere study. Am J Hum Genet, 2002, 71: 695–714
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