小麦茎秆木质素代谢及其与抗倒性的关系

doi:10.3724/SP.J.1006.2011.01616

Abstract

Abstract: As a component of cell wall, lignin is believed to be relevant to mechanical intensity of culm. In this study, we investigated the dynamic changes of lignin content and activities of four enzymes involved in lignin metabolism in culm as well as the snapping resistance and lodging index of wheat (Tritcum aestivum L.) to disclose the relationship between lignin content and lodging resistance. The lignin content and activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), cinnamyl alcohol dehydrogenase (CAD), and 4-coumarate: CoA ligase (4CL) were tested with the second internode of six cultivars with different lodging resistance. The snapping resistance and culm lodging index were determined at anthesis, milking, and maturity stages. The lignin content varied significantly among cultivars. Jimai 22 and Jimai 20, which showed high resistance to culm snapping and loging, had higher lignin content and the activities of PAL, TAL, CAD, and 4CL than other cultivars. The lignin content was negatively correlated with the actual lodging ratio (r = -0.83, P < 0.05), and positively correlated with the snapping resistance of culm (r = 0.86, P < 0.05). The activities of PAL, TAL, and CAD were positively correlated with lignin content with correlation coefficients of 0.78 (P < 0.05), 0.77 (P < 0.05) and 0.85 (P < 0.05), respectively. Based on these results, we conclude that high activities of PAL and CAD are the enzymatic basis of increased lignin content, and the lignin content in culm can be used as an important indicator to evaluate lodging resistance of wheat.

Key words: Winter wheat, Lignin metabolism, Lodging resistance, Lodging index, Enzyme activity

CHEN Xiao-Guang, SHI Chun-Tu, YIN Yan-Ping, WANG Zhen-Lin, SHI Yu-Hua, PENG Dian-Liang, NI Yang-Li, CAI Tie. Relationship between Lignin Metabolism and Lodging Resistance in Wheat[J].Acta Agron Sin, 2011, 37(09): 1616-1622.

References

[1]Yao R-L(姚瑞亮), Zhu W-X(朱文祥). The correlation analysis of the stem traits and lodging in wheat. J Guangxi Agric Univ (广西农业大学学报), 1998, 17(12): 16–18 (in Chinese with English abstract)
[2]Dong Q(董琦), Wang A-P(王爱萍), Liang S-M(梁素明). Study on the architectural characteristics of wheat stalks. J Shanxi Agric Univ (山西农业大学学报), 2003, 23(3): 188–191 (in Chinese with English abstract)
[3]Zhu X-K(朱新开), Wang X-J(王祥菊), Guo K-Q(郭凯泉), Guo W-S(郭文善), Feng C-N(封超年), Peng Y-X(彭永欣). Stem characteristics of wheat with stem lodging and effects of lodging on grain yield and quality. J Triticeae Crops (麦类作物学报), 2006, 26(1): 87–92 (in Chinese with English abstract)
[4]Zahour A, Rasmusson D C, Gallagher L W. Effects of semi-dwarf stature, head number, and kernel number on grain yield in barley in morocco. Crop Sci, 1987, 27: 161–165
[5]Yuan Z-H(袁志华), Feng B-P(冯宝萍), Zhao A-Q(赵安庆), Liang A-Q(梁爱琴). Dynamic analysis and comprehensive evaluation of crop-stem lodging resistance. Trans CSAE (农业工程学报), 2002, 18(6): 30–31 (in Chinese with English abstract)
[6]Yuan Z H, Li Y J. Relationship between bending property and density of wheat stem. Agric Sci Technol, 2009, 10(5): 100–101
[7]Wang F-E(王芬娥), Huang G-B(黄高宝), Guo W-J(郭维俊), Zhang F-W(张锋伟), Wu J-M(吴建民), Zhao D-J(赵多佳). Mechanical properties and micro-structure of wheat stems. Trans Chin Soc Agric Machinery (农业机械学报), 2009, 40(5): 92–95 (in Chinese with English abstract)
[8]Wei F-Z(魏凤珍), Li J-C(李金才), Wang C-Y(王成雨), Qu H-J(屈会娟), Shen X-S(沈学善). Effects of nitrogenous fertilizer application model on culm lodging resistance in winter wheat. Acta Agron Sin (作物学报), 2008, 34(6): 1080–1085 (in Chinese with English abstract)
[9]Zhu L, Qian Q, Zhou Y H, Yan M X, Sun L, Zhang M, Fu Z-M, Wang Y-H, Han B, Pang X M, Chen M S, Li J Y. Brittle culm1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants. Plant Cell, 2003, 15: 2020–2031
[10]Kokubo A, Sakurai N, Kuraishi S, Takeda K. Culm brittleness of barley (Hordeum vulgare L.) mutants is caused by smaller number of cellulose molecules in cell wall. Plant Physiol, 1991, 97: 509–514
[11]Li J-C(李金才), Yin J(尹钧), Wei F-Z(魏凤珍). Effects of planting density on characters of culm and culm lodging resistant index in winter wheat. Acta Agron Sin (作物学报), 2005, 31(5): 662–666 (in Chinese with English abstract)
[12]Guo W-J(郭维俊), Wang F-N(王芬娥), Huang G-B(黄高宝), Zhang F-W(张锋伟), Wei S-L(魏时来). Experiment on mechanical properties and chemical compositions of wheat stems. Trans Chin Soc Agric Machinery (农业机械学报), 2009, 40(2): 110–114 (in Chinese with English abstract)
[13]Zhang K-P(张坤普), Zhao L(赵亮), Hai Y(海燕), Chen G-F(陈广凤), Tian J-C(田继春). QTL mapping for adult-plant resistance to powdery, lodging resistance and internode length below spike in wheat. Acta Agron Sin (作物学报), 2008, 34(8): 1350–1357 (in Chinese with English abstract)
[14]Ma Q H. The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance. J Exp Bot, 2009, 60: 2763–2771
[15]Ma Q H. Functional analysis of a cinnamyl alcohol dehydrogenase involved in lignin biosynthesis in wheat. J Exp Bot, 2010, 61: 2735–2744
[16]Jones L, Ennos A R, Turner S R. Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis. Plant J, 2001, 26: 205–216
[17]Welton F A. Lodging in oats and wheat. Bot Gaz, 1928, 85: 121–151
[18]Ookawa T, Ishihara K V. Difference of the cell wall components affecting the ding stress of the culm in relating to the lodging resistance in paddy rice. Jpn J Crop Sci, 1993, 62: 378–384
[19]Tripathi S C, Sayre K D, Kaul J N. Growth and morphology of spring wheat (Triticum aestivum L.) culms and their association with lodging: effects of genotypes, N levels and ethephon. Field Crops Res, 2003, 84: 271–290
[20]Lin K(林葵), Huang X-H(黄祥辉), Wang L-H(王隆华), Li R-G(李人圭), Yan J-Q(颜季琼). The change of PAL activity and lignin content during adventitious buds formation of cotyledon in Cucumis melon L. J East China Normal Univ (Nat Sci) (华东师范大学学报?自然科学版), 1996, (2): 92–97 (in Chinese with English abstract)
[21]Zhang Z-L(张志良), Qu W(瞿伟). Experimental Guide for Plant Physiology (植物生理学实验指导). Beijing: Higher Education Press, 2003 (in Chinese)
[22]Liu X-Y(刘晓燕), Jin J-Y(金继运), He P(何萍), Liu H-L(刘海龙), Li W-J(李文娟). Preliminary study on the relation between potassium chloride suppressing corn stalk rots and soil microorganism characteristics. Plant Nutr Fert Sci (植物营养与肥料学报), 2007, 13(2): 279–285 (in Chinese with English abstract)
[23]Knobloch K H, Hahlbrock K. Isoenzymes of p-coumarate: CoA ligase from cell suspension cultures of Glycine max. Eur J Biochem, 1975, 52: 311–320
[24]Morrison T A, Kessler J R, Hatfield R D, Buxton D R. Activity of two lignin biosynthesis enzymes during development of a maize internode. J Sci Food Agric, 1994, 65: 133–139
[25]Turner S R, Somerville C R. Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell, 1997, 9: 689–701
[26]Lewis N G, Yamamoto E. Lignin: occurrence, biogenesis and biodegradation. Annu Rev Plant Physiol Plant Mol Biol, 1990, 41: 455–496
[27]Baucher M, Monties B, Van Montagu M, Boerjan W. Biosynthesis and genetic engineer in lignin. Critical Rev Plant Sci, 1998, 17: 125–197
[28]Berry P M, Spink J, Sterling M, Pickett A A. Methods for rapidly measuring the lodging resistance of wheat cultivars. J Agron Crop Sci, 2003, 189: 390–401
[29]Tripathi S C, Sayre K D, Kaul J N, Norden A J, Frey K J. Growth and morphology of spring wheat (Triticum aestivum L.) culms and their association with lodging: effects of genotypes, N levels and ethephon. Field Crops Res, 2003, 84: 271–290
[30]Mohammadi M, Kazemi H. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Sci, 2002, 162: 491–498
[31]Luo Z-S(罗自生). Effect of GA3 treatment on lignification and endogenous hormone levels of postharvest bamboo shoots. Acta Hortic Sin (园艺学报), 2005, 32(3): 454–457 (in Chinese with English abstract)
[32]Boudet A M, Kajita S, Grima-Pettenati J. Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends Plant Sci, 2003, 8: 576–581

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Relationship between Lignin Metabolism and Lodging Resistance in Wheat

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