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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 159-167.doi: 10.3724/SP.J.1006.2012.00159

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Changes in Starch Accumulation and Activity of Enzymes Associated with Starch Synthesis under different Nitrogen Applications in Japonica Rice in Cold Region

DU Xiao-Dong,ZHAO Hong-Wei*,WANG Jing-Guo,LIU Hua-Long,YANG Liang,XU Jing,SONG Jin-Tong?   

  1. Rice Research Institute, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
  • Received:2011-03-31 Revised:2011-07-25 Online:2012-01-12 Published:2011-11-07
  • Contact: 赵宏伟, E-mail: hongweizhao@163.com

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Abstract: The effect of different nitrogen application regimes on rice starch accumulation, starch biosynthetic enzyme activity and the relationship between them after flowering was studied under same N amount with different application periods time, and Songjing 6 and Songjing 9. The results showed that reducing the proportion of base nitrogen fertilizer and increasing the nitrogen fertilizer proportion in grain killing period could promote grain filling, increase total starch content, and appropriately increase amylopectin content. Under different nitrogen applications, the trends and the peak activities of changed of key enzymes associated with starch synthesis were different. In grain filling stage, the activities of ADPG pyrophosphorylase and Q enzyme could be improved significantly by reducing base N and increasing ear-N. In the early grain filling stage, the SSS activity was decreased with decreasing the ratio face fertilizer to tillering fertilizer. the activities of ADPG pyrophosphorylase, Q enzyme, and SSS were significantly correlated the content of starch. Fertilizer application ratio influenced both the content and the composition of starch. Reasonable fertilizer application ratio (5:3:1:1, at processing stage, tillering stage, panicle stage and grain filling stage) could regulate the activities of key enzymes for starch synthesis in different stages and increase contents of starch and starch components.

Key words: Japonica rice in cold region, Nitrogen application, Starch, Starch accumulation, Key enzymes of starch synthesis

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