Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 159-167.doi: 10.3724/SP.J.1006.2012.00159


Changes in Starch Accumulation and Activity of Enzymes Associated with Starch Synthesis under different Nitrogen Applications in Japonica Rice in Cold Region

DU Xiao-Dong,ZHAO Hong-Wei*,WANG Jing-Guo,LIU Hua-Long,YANG Liang,XU Jing,SONG Jin-Tong?   

  1. Rice Research Institute, College of Agriculture, Northeast Agricultural University, Harbin 150030, China
  • Received:2011-03-31 Revised:2011-07-25 Online:2012-01-12 Published:2011-11-07
  • Contact: 赵宏伟, E-mail: hongweizhao@163.com

Abstract: The effect of different nitrogen application regimes on rice starch accumulation, starch biosynthetic enzyme activity and the relationship between them after flowering was studied under same N amount with different application periods time, and Songjing 6 and Songjing 9. The results showed that reducing the proportion of base nitrogen fertilizer and increasing the nitrogen fertilizer proportion in grain killing period could promote grain filling, increase total starch content, and appropriately increase amylopectin content. Under different nitrogen applications, the trends and the peak activities of changed of key enzymes associated with starch synthesis were different. In grain filling stage, the activities of ADPG pyrophosphorylase and Q enzyme could be improved significantly by reducing base N and increasing ear-N. In the early grain filling stage, the SSS activity was decreased with decreasing the ratio face fertilizer to tillering fertilizer. the activities of ADPG pyrophosphorylase, Q enzyme, and SSS were significantly correlated the content of starch. Fertilizer application ratio influenced both the content and the composition of starch. Reasonable fertilizer application ratio (5:3:1:1, at processing stage, tillering stage, panicle stage and grain filling stage) could regulate the activities of key enzymes for starch synthesis in different stages and increase contents of starch and starch components.

Key words: Japonica rice in cold region, Nitrogen application, Starch, Starch accumulation, Key enzymes of starch synthesis

[1]FAO. Rice in Human Nutrition. Laguna, Philippines: International Rice Research Institute, 1993. pp 35−60
[2]Emes M J, Bowsher C G, Hedley C, Burrell M M, Scrase-Field E S F, Tetlow I J. Starch synthesis and carbon partitioning in developing endosperm. J Exp Bot, 2003, 54: 569−575
[3]Wan L-J(万靓军), Zhang H-C(张洪程), Huo Z-Y(霍中洋), Lin Z-C(林忠成), Dai Q-G(戴其根), Xu K(许轲), Zhang J(张军). Effects of nitrogen application regimes on yield qualityand nitrogen use efficiency of super japonica hybrid rice. Acta Agron Sin (作物学报), 2007, 33(2): 175−182 (in Chinese with English abstract)
[4]Zuo Z-P(左振朋), Wang J(王婧), Dong L-H(董鲁浩), Ma D-C(马登超), Sun Q-Q(孙庆泉), Dong S-T(董树亭). Comparison of multiplication of endosperm cell and physiological activity in developing kernels among normal corn, glutinous corn and pop corn. Acta Agron Sin (作物学报), 2010, 36(4): 630−636 (in Chinese with English abstract)
[5]Ma J(马均), Ming D-F(明东风), Ma W-B(马文波), Xu F-Y(许凤英). Changes in starch accumulation and activity of enzymes associated with starch synthesis under different n supplying date. Sci Agric Sin (中国农业科学), 2005, 38(1): 52−58 (in Chinese with English abstract)
[6]Jia S-F(贾士芳), Dong S-T(董树亭), Wang K-J(王空军), Zhang J-W(张吉旺), Li C-F(李从锋). Effect of shading on kernel quality at different stages from flowering to maturity in maize. Acta Agron Sin (作物学报), 2007, 33(12): 1960−1967 (in Chinese with English abstract)
[7]Sun Q-Q(孙庆泉), Wu Y-Q(吴元奇), Hu C-H(胡昌浩), Dong S-T(董树亭), Rong T-Z(荣廷昭), Zhang Y(张颖). Physiological activities and multiplication of endosperm cell at filling stage of kernels with different yield potential in maize. Acta Agron Sin (作物学报), 2005, 31(5): 612−618 (in Chinese with English abstract)
[8]Li S-C(李绍长), Lu J-H(陆嘉惠), Meng B-M(孟宝民). The relationship between endosperm cell differentiating and kernel filling. J Maize Sci (玉米科学), 2000, 8(4): 45−47 (in Chinese with English abstract)
[9]Cheng F-M(程方民), Jiang D-A(蒋德安), Wu P(吴平), Shi C-H(石春海). The dynamic change of starch synthesis enzymes during the kernel filling stage and effects of temperature upon it. Acta Agron Sin (作物学报), 2001, 27(2): 201−206 (in Chinese with English abstract)
[10]Yang J C, Peng S B, Gu S L, Visperas R M, Zhu Q S. Changes in activities of three enzymes associated with starch synthesis in rice grains during grain filling. Acta Agron Sin (作物学报), 2001, 27(2): 157-164 (in Chinese with English abstract)
[11]S hi H-R(史鸿儒), Zhang W-Z(张文忠),Xie W-X(解文孝), Yang Q(杨庆), Zhang Z-Y(张振宇), Han Y-D(韩亚东), Xu Z-J(徐正进), Chen W-F(陈温福). Analysis of matter production characteristics under different nitrogen application patterns of japonica super rice in North China. Acta Agron Sin (作物学报), 2008, 34(11): 1985−1993 (in Chinese with English abstract)
[12]Douglas C D, Tsung M K, Frederick C F. Enzymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize. Plant Physiol, 1988, 86: 1013−1019
[13]Cheng F-M(程方民), Jiang D-A(蒋德安), Wu P(吴平), ShiC-H(石春海). The dynamic change of starch synthesis enzymes during the kernel filling stage and effects of temperature upon it. Acta Agron Sin (作物学报), 2001, 27(2): 201−206 (in Chinese with English abstract)
[14]Li T-G(李太贵), Shen B(沈波), Chen N(陈能), Luo Y-K(罗玉坤). Effect of Q-enzyme on the chalkiness formation of rice grain. Acta Agron Sin (作物学报), 1997, 23(3): 338-344 (in Chinese with English abstract)
[15]He Z-F(何照范). Grain Quality and Its Analysis Technology (粮油籽粒品质及其分析技术). Beijing: Agriculture Press, 1985. pp 274−294 (in Chinese)
[16]Nakamura Y, Yuki K, Park S Y. Carbohydate metabolis in the developing endosperm of rice grains. Plant Cell Physiol, 1989, 30: 833−839
[17]Zhao B H, Zhang W J, Wang Z Q, Zhu Q S, Yang J C. Changes in activities of the key enzymes related to starch synthesis in rice grains during grain filling and their relationships with the filling fate and cooking quality. Agric Sci China, 2005, 4(1): 26−33
[18]Pan X-H(潘晓华), Li M-Y(李木英), Cao J-M(曹聚明). Starch accumulation in developing endosperm of rice and changes in activities of enzymes associated with starch synthesis. J Jiangxi Agric Univ (江西农业大学学报), 1999, 21(4): 456−462
[19]Wang W-J(王文静). The relationship between source sink intensity and starch accumulation during grain filling period in two winter wheat cultivars with different spike types. Acta Agron Sin (作物学报), 2004, 30(9): 916−921 (in Chinese with English abstract)
[20]Mengel K, Judel G K. Effect of shading on nonstructural carbohydrate sand their turnover in culms and leaves during the grain filling period of spring wheat. Crop Sci, 1982, 22: 958−962
[21]Wang W(王维), Cai Y-X(蔡一霞), Cai K-Z(蔡坤争), Zhang J-H(黄张建华), Yang J-C(杨建昌), Zhu Q-S(朱庆森). Effect of controlled soil drying on grain filling and activities of key enzyme starch synthesis in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2006, 32(7): 972−979 (in Chinese with English abstract)
[22]Huang F-S(黄发松), Sun Z-X(孙宗修), Hu P-S(胡培松), Tang S-Q(唐绍清). Present situations and prospects for the research on rice grain quality forming. Chin J Rice Sci (中国水稻科学), 1998, 12(3): 172−176
[23]Sun Q-Q(孙庆泉), Hu C-H(胡昌浩), Dong S-T(董树亭), Wang K-J(王空军). Advances of physiological characters studies about leaf source and kernel sink on cultivars in maize. J Shandong Agric Univ (山东农业大学学报), 1999, 30(4): 484−492 (in Chinese with English abstract)
[1] PENG Xi-Hong, CHEN Ping, DU Qing, YANG Xue-Li, REN Jun-Bo, ZHENG Ben-Chuan, LUO Kai, XIE Chen, LEI Lu, YONG Tai-Wen, YANG Wen-Yu. Effects of reduced nitrogen application on soil aeration and root nodule growth of relay strip intercropping soybean [J]. Acta Agronomica Sinica, 2022, 48(5): 1199-1209.
[2] YUAN Jia-Qi, LIU Yan-Yang, XU Ke, LI Guo-Hui, CHEN Tian-Ye, ZHOU Hu-Yi, GUO Bao-Wei, HUO Zhong-Yang, DAI Qi-Gen, ZHANG Hong-Cheng. Nitrogen and density treatment to improve resource utilization and yield in late sowing japonica rice [J]. Acta Agronomica Sinica, 2022, 48(3): 667-681.
[3] CHEN Yun, LIU Kun, ZHANG Hong-Lu, LI Si-Yu, ZHANG Ya-Jun, WEI Jia-Li, ZHANG Hao, GU Jun-Fei, LIU Li-Jun, YANG Jian-Chang. Effects of machine transplanting density and panicle nitrogen fertilizer reduction on grains starch synthesis in good taste rice cultivars [J]. Acta Agronomica Sinica, 2021, 47(8): 1540-1550.
[4] YANG Zhi-Yuan, SHU Chuan-Hai, ZHANG Rong-Ping, YANG Guo-Tao, WANG Ming-Tian, QIN Jian, SUN Yong-Jian, MA Jun, LI Na. Comparison of tolerances to nitrogen fertilizer between compact and loose hybrid indica rice varieties [J]. Acta Agronomica Sinica, 2021, 47(8): 1593-1602.
[5] YANG Fan, ZHONG Xiao-Yuan, LI Qiu-Ping, LI Shu-Xian, LI Wu, ZHOU Tao, LI Bo, YUAN Yu-Jie, DENG Fei, CHEN Yong, REN Wan-Jun. Effects of delayed sowing and planting date on starch RVA profiles of different indica hybrid rice in the sub-suitable region of ratoon rice [J]. Acta Agronomica Sinica, 2021, 47(4): 701-713.
[6] DONG Er-Wei, WANG Jin-Song, WU Ai-Lian, WANG Yuan, WANG Li-Ge, HAN Xiong, GUO Jun, JIAO Xiao-Yan. Effects of row space and plant density on characteristics of grain filling, starch and NPK accumulation of sorghum grain of different parts of panicle [J]. Acta Agronomica Sinica, 2021, 47(12): 2459-2470.
[7] HUANG Su-Hua, LIN Xi-Yue, LEI Zheng-Ping, DING Zai-Song, ZHAO Ming. Physiological characters of carbon, nitrogen, and hormones in ratooning rice cultivars with strong regeneration ability [J]. Acta Agronomica Sinica, 2021, 47(11): 2278-2289.
[8] SHI Xiao-Juan, HAN Huan-Yong, WANG Fang-Yong, HAO Xian-Zhe, GAO Hong-Yun, LUO Hong-Hai. Effects of chemical topping with fortified mepiquat chloride on photosynthetic characteristics of cotton leaves under different nitrogen rates [J]. Acta Agronomica Sinica, 2020, 46(9): 1416-1429.
[9] TANG Jian,TANG Chuang,GUO Bao-Wei,ZHANG Cheng-Xin,ZHANG Zhen-Zhen,WANG Ke,ZHANG Hong-Cheng,CHEN Heng,SUN Ming-Zhu. Effect of nitrogen application on yield and rice quality of mechanical transplanting high quality late rice [J]. Acta Agronomica Sinica, 2020, 46(01): 117-130.
[10] Li-Na JIANG,Jing-Li MA,Bao-Ting FANG,Jian-Hui MA,Chun-Xi LI,Zhi-Min WANG,Bao-Zhen HAO. Effect of lower water and nitrogen supply on grain yield and dry matter remobilization of organs in different layers of winter wheat plant in northern Henan province [J]. Acta Agronomica Sinica, 2019, 45(6): 957-966.
[11] Fang NING,Yuan-Hong ZHANG,Peng-Fei WEN,Rui WANG,Qian WANG,Zhao-Yang DONG,Guang-Can JIA,Jun LI. Responses of maize growth and yield to nitrogen application in dryland under different precipitation conditions [J]. Acta Agronomica Sinica, 2019, 45(5): 777-791.
[12] YU Hai-Yue,HAN Zi-Xuan,ZHANG Yu-Shi,DUAN Liu-Sheng,ZHANG Ming-Cai,LI Zhao-Hu. Regulation of coronatine on the grain filling characteristics and starch synthesis in maize kernels [J]. Acta Agronomica Sinica, 2019, 45(10): 1535-1543.
[13] Sha-Sha LI,Geng MA,Wei-Xing LIU,Juan KANG,Yu-Lu CHEN,Yang-Yang HU,Pan-Pan ZHANG,Chen-Yang WANG. Effects of Long-Term Irrigation and Nitrogen Regimes on Soil Nitrogen Content and Paste Property of Wheat Grain [J]. Acta Agronomica Sinica, 2018, 44(7): 1067-1076.
[14] Chun-Yan LI,Wen-Xia ZHANG,Yu-Xue ZHANG,Meng-Hao YAO,Jin-Feng DING,Xin-Kai ZHU,Wen-Shan GUO,Chao-Nian FENG. Differences in Physicochemical Properties between Flour and Starch of Wheat Grain [J]. Acta Agronomica Sinica, 2018, 44(7): 1077-1085.
[15] Yang-Yang HU, Hong-Fang LU, Wei-Xing LIU, Juan KANG, Geng MA, Sha-Sha LI, Ying-Ying CHU, Chen-Yang WANG. Effects of High Temperature and Water Deficiency during Grain Filling on Activities of Key Starch Synthesis Enzymes and Starch Accumulation in Wheat [J]. Acta Agronomica Sinica, 2018, 44(04): 591-600.
Full text



No Suggested Reading articles found!