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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 2015-2023.doi: 10.3724/SP.J.1006.2012.02015

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Inheritance of Sterility in Genic Male Sterile Line (20118A) and Marker-Assisted Selection in Hybrid Breeding of Brassica napus L.

REN Meng-Yang1,2,NI Xi-Yuan2,WANG Hao3,CHEN Fei2,TIAN Jian-Hua3,HUANG Ji-Xiang2,LI Dian-Rong3,ZHAO Jian-Yi2,*   

  1. 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 Hybrid Rapeseed Research Center of Shaanxi Province, Dali 715105, China
  • Received:2012-02-11 Revised:2012-04-16 Online:2012-11-12 Published:2012-05-11
  • Contact: 赵坚义, E-mail: jyzhao3@yahoo.com, Tel: 0571-86403406 E-mail:yun2006717@163.com

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Abstract:

In the present study, twenty varieties/lines of Brassica napus were test-crossed with sterile lines 20118A, and its temporary maintainer 20118A-TAM. Both traditional genetic analysis and molecular marker assisted technology were employed to confirm their genetic model of sterility with two gene control system, to check the allelic distributions among normal rapeseed varieties or lines. Furthermore, the efficiency and accuracy of marker-assisted selection (MAS) for two-type line and temporary maintainers based on co-dominant markers were also investigated. The results showed that the segregation proportion of male fertile to sterile plants in F2 progenies from six varieties (lines) crossed with 20118A fitted mendelian segregation (3:1 and 13:3), and that with 20118A-TAM showed either 13:3 or full male fertile, indicating that the sterility of 20118Ais controlled by one recessive sterile gene interacting with a recessive epistatic suppression gene. In addition, a reverse validation approach based on Bnms3 and Bnrf linked marker assisted selection was used to further confirm the two gene control system. From a total of 1059 F2 plants, 70, 69 and 135 individuals carrying temporary maintainer(ms3ms3rfrf), homozygous sterile (ms3ms3RfRf) and fertile (Ms3ms3RfRf) marker genotypes were screened out, respectively, which also fitted the Mendelian segregation proportions of two gene model (1/16, 1/16, and 1/8). After test-crossing with known homozygous sterile plants or temporary maintainers or one another among marker genotypes, higher than 95% of lines were approved to be the expected genotypes. Finally, according to the information from 20 testcross cultivars/lines, only two alleles of Rf and rf were observed in BnRf locus, implying the third allele naturally existed very few, if any. Therefore, for practical breeding purpose, a marker assisted selection strategy simply based on BnRf/rf and BnMs3/ms3linked co-dominant markers is proposed.

Key words: Brassica napus L., Recessive genic male sterility, Molecular marker assisted selection, Two-type line, Temporary maintainer

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