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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 494-505.doi: 10.3724/SP.J.1006.2013.00494

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Water Shortage and Increasing Nitrogen Application on Photosynthetic Function of Different Hybrid Rice Combinations at Grain Filling Stage

WU Zi-Ming,WANG Zhu-Qing,LI Mu-Ying*,ZENG Lei,SHI Qing-Hua,PAN Xiao-Hua,TAN Xue-Ming   

  1. Key Laboratory of Crop Physiology Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2012-08-06 Revised:2012-11-16 Online:2013-03-12 Published:2013-01-04
  • Contact: 李木英, E-mail: myli53@yahoo.com.cn, Tel: 0791-83813877

Abstract:

The response of photosynthetic function of leaves in aging process to water shortage and additional nitrogen application were studied using premature senescence type and non-premature senescence type of hybrid rice combinations. The results showed that chlorophyll content, leaf area, Rubisco activity and photosynthetic rate decreased, and ZR (zeatin riboside) content declined by water deficiency. There were significantly different responses to water shortage among different combinations. Under water stress, Zhongyou 838, Tianyou 998, and Longping 601 were more sensitive than others, and their chlorophyll decline rate increased by 9–12%, whereas photosynthetic rate decreased by 19.55%, 19.12%, and 12.73% respectively, and their ZR content, Rubisco activity dropped much more than those of other combinations, Under water shortage, additional nitrogen application rate could relieve the effect of water shortage stress, the decline rate of chlorophyll decreased by 6.14–27.10%, photosynthetic rate increased by 9.30–15.81% for Longping 601, Tianyou 998, Xianyou 63, and Zhongyou 838, and 3–5% for Liangyou 6326 and C Liangyou 396. And Rubisco activity was enhanced by 18.94% in Longping 601, 12.16% in Zhongyou 838, and 3–9% in other combinations. Additional nitrogen application increased ZR content by 16.05% in Zhongyou 838, 8.31% and 8.16% in C Liangyou 396 and Xianyou 63, and 4.90% and 1.13% in Liangyou 6326 and Longping 601.The correlation analysis showed that ZR content was positively correlated with, photosynthetic rate and Rubisco activity. Rubisco activity and photosynthetic rate had a positive correlation with chlorophyll content, and content of total sugars and yield were significantly correlated with photosynthetic rate.The conclusion was that water shortage speeds up the aging and the decline photosynthetic function decrease. Increasing moderate nitrogen fertilizer will delay the premature senescence of leaves caused by drought stress, enhance the photosynthetic function, and extend photosynthetic function period, suggesting that N nutrition is the most important factor for regulating rice leaf senescence.

Key words: Hybrid rice, Senescence, Water shortage, Additional nitrogen application, Photosynthetic function

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