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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (03): 520-529.doi: 10.3724/SP.J.1006.2013.00520

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Photosynthetic Characteristics and Chlorophyll Fluorescence Kinetic Parameters Analyses of Chlorophyll-Reduced Mutant in Brassica napus L.

XIAO Hua-Gui1,2,YANG Huan-Wen1,*,RAO Yong2,*,YANG Bin2,ZHU Ying3   

  1. 1 College of Tobacco Science, Yunnan Agricultural University, Kunming 650201, China; 2 Guizhou Institute of Oil Crops, Guiyang 550006, China; 3 Guizhou Institute of Biological Technology, Guiyang 550006, China
  • Received:2012-07-27 Revised:2012-11-16 Online:2013-03-12 Published:2013-01-04
  • Contact: 杨焕文, E-mail: mryanghuanwen@126.com, Tel: 0871-5227816; 饶勇, E-mail: ry590@21cn.com E-mail:xiaohuagui74@21cn.com

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Abstract:

We investigatedthe photosynthetic pigment contents, photosynthetic characteristics, chlorophyll fluorescence kinetic parameters and agronomic traits at five-leaf stage of the chlorophyll-reduced mutant (NY), wild-type (NG), F1 and rF1 of their combinations (reciprocal cross). The results showed that the chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in the mutant were significantly reduced compared with there in other materials, expecially for chlorophyll b. The net photosynthetic rate (Pn) of the mutant was significantly lower than that of wild-type and their F1 and rF1. Relatively high intercellular CO2 concentration (Ci) and an equivalent stomatal conductance (Gs) in the mutant indicated that stomatal factor was not the limiting factor of the photosynthetic rate. The mutant had higher light compensation point (LCP) and light saturation point (LSP), and its dark respiration rate (Rd) was equal to that of wild-type, whereas apparent quantum yield (AQY) and quantum yield at light compensation point (φc) in the mutant were significantly decresed. The CO2 compensation point (CCP), rate of photorespiration (Rp) and carboxylation efficiency (CE) of the mutant NY were significantly lower than those of the wild-type NG and their combinations of NY×NG and NG×NY, but the mutant had higher CO2 saturation point (CSP). The fluorescence parameters in the mutant, including Fo, Fm, Fv/Fm, Fv'/Fm', ΦPSII, qp, NPQ, and ETR were significantly reduced. Lower photosynthetic pigment content may be the main reason for low leaf photosynthetic rate which directly leads to the lower light-harvesting capacity and photochemical conversion efficiencies of the PSII reaction center. Moreover, lasting etiolation of leaves in the mutant had a greater impact on its growth and development, but the photosynthetic characteristics and agronomic traits were restored to normal level in F1 of the cross between the mutant and the normal parent.

Key words: Brassica napus L., Chlorophyll-reduced mutant, Photosynthetic pigment content, Photosynthetic characteristics, Chlorophyll fluorescence kinetic parameters

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