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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1521-1530.doi: 10.3724/SP.J.1006.2014.01521

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

An Evaluation System for Rice Black-Streaked Dwarf Virus Disease and Screening for Resistant Rice Germplasm

WANG Bao-Xiang1,HU Jin-Long2,SUN Zhi-Guang2,SONG Zhao-Qiang2,LU Bai-Guan1,ZHOU Zhen-Ling1,FAN Ji-Wei1,QIN De-Rong1,LIU Yu-Qiang2,JIANG Ling2,XU Da-Yong1,*,WAN Jian-Min2,3,*   

  1. 1 Institute of Lianyungang Agricultural Science of Xuhuai Area, Lianyungang 222006, China; 2 State Key Laboratory of Crop Genetics and Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2014-02-19 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-10
  • Contact: 万建民, E-mail: wanjm@njau.edu.cn, Tel: 025-84396516; 徐大勇, E-mail: xudayong3030@sina.com, Tel: 0518-85589316

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Abstract:

Rice black-streaked dwarf virus disease (RBSDVD) is one of the most serious viral diseases. The absence of efficient resistance evaluation system for RBSDVD restricts the excavation of the resistance resource, thereby the resistance breeding and basal researches are limited. Here, we proposed that the appropriate density of small brown planthopper (SBPH) should be 8 million ha-1 and the rate of RBSDV-carrying SBPH should be over 5% for RBSDVD evaluation in field condition. Further, we optimized the artificial inoculation evaluation system including circulative period, number of SBPH per plant, infestation time and age of SBPH. We evaluated 1240 varieties from 21 countries for RBSDV resistance by natural inoculation in 2010, 34 cultivars had the disease incidence less than 10%. The 34 cultivars were further evaluated by natural inoculation in three different locations in 2011 and 2012, respectively. Finally, only three varieties, Kanyakumari 29, Madurai 25, and Vietnam 160, which all came from Southeast Asia, had the consistent disease incidence less than 10% in different locations and years. To verify the resistance of the three varieties against RBSDVD, we carried out the interval sowing inoculation experiment in the three different locations in 2012. In the interval sowing experiment, the disease incidence of Kanyakumari 29 was less than 12%, whereas Madurai 25 and Vietnam 160 less than 9%. In addition, in the artificial field inoculation and artificial room inoculation experiments, the disease incidence of the three varieties was less than 9%. The above natural and artificial inoculation experiments showed that Kanyakumari 29, Madurai 25, and Vietnam 160 performed stable and high RBSDVD resistance. Taken together, the RBSDVD evaluation system combining field with artificial infestation identifications used in this study is accurate and reliable. This system could be used to widely screen RBSDVD resistance resources. The construction of RBSDVD evaluation system and the RBSDVD resistance resources identified in this study will provide the useful tools and materials for the identification of RBSDVD resistance gene(s) and development of RBSDVD resistance varieties.

Key words: Rice (Oryza sativa L.), Rice black-streaked dwarf virus, Resistance identification

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