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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1658-1666.doi: 10.3724/SP.J.1006.2014.01658

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Impact of IAA, GA3, and ABA on Negative Root Phototropism and Root Growth of Rice

LIU Da-Tong,JING Yan-Ping,SHI Hai-Xiang,ZHONG Ting-Ting,WANG Zhong*   

  1. College of Bioscience and Biotechnology, Yangzhou University / Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou 225009, China
  • Received:2014-01-29 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-10
  • Contact: 王忠, E-mail: wangzhong@yzu.edu.cn

Abstract:

The objective of this research was to investigate the relationship between rice roots negative phototropism and endogenous plant hormone levels, as well as the impact of exogenous hormones on rice root growth. The adventitious roots and primary roots of conventional cultivars Yangdao6 (indica), Nipponbare (japonica), Zhonghua11 (japonica) and its OsPIN1a over expressed transgenic seedlings were used to observe the process of root negative phototropism, measure contents of endogenous auxin (IAA), gibberellin (GA3) and abscisic acid (ABA) in the bending part of root tips, and investigate the impact of exogenous hormones to rice root morphology and anatomy. Results indicated that seedling roots of both Yangdao6 and Nipponbare had negative phototropism, and the lateral roots and adventitious roots of Yangdao6 showed stronger negative phototropism. Plant hormones and negative phototropism of rice root and root development are closely linked. Under unilateral illumination, endogenous IAA, GA3 and ABA levels declined. The content of these phytohormones in the irradiated side was lower than that in the shaded side. The treatment of unilateral light and exogenous IAA induced a large number of root hairs in Yangdao6. Compared with conventional rice, the angel of negative phototropism was lager in OsPIN1a transgenic plant. There was no direct relationship between exogenous GA3 and ABA treatments and root negative phototropism of rice. Exogenous ABA inhibited root growth obviously. The total absorption surface area, root number and root length per plant were significantly reduced by ABA treatment. The treatment of 10 μmol L-1ABA not only caused a dysplasia of both meristematic and elongation zone but also promoted the development of the central vascular tissue in root tips.

Key words: Rice, Root negative phototropism, IAA, GA3, ABA

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