Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2192-2197.doi: 10.3724/SP.J.1006.2014.02192

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Sequence Characteristics and Expression of NAD-malic enzyme in Amaranthus hypochondriacus L.

BAI Yun-Feng1,3,NIE Jiang-Ting2,ZHANG Zhong-Liang1,LI Ping1,ZHANG Wei-Feng1,YAN Jian-Jun1,FENG Rui-Yun1,ZHANG Yao1   

  1. 1 Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China; 2 College of Bioengineering, Shanxi University, Taiyuan 030006, China; 3 China Key Laboratory of Loess Plateau Crop Gene Resources and Germplasm Creation, Ministry of Agriculture, Taiyuan 030006, China
  • Received:2014-02-25 Revised:2014-09-16 Online:2014-12-12 Published:2014-09-26

Abstract:

The NAD(P)-malic enzyme (NAD(P)-ME) found in many metabolic pathways catalyzes the oxidative decarboxylation of L-malate, which results in producing pyruvate, CO2 and NAD(P)H. In C4 plants, NAD(P)-ME plays a key role in photosynthetic carbon fixation. This study was aimed to characterize the AhNAD-ME in dicotyledonous C4 Amaranthus hypochondriacus by sequence analysis, examine the expression patterns of AhNAD-ME gene in different tissues and different durations of illumination time, and construct a recombinant plasmid pEASY-E1 harboring the AhNAD-ME cDNA and then transform the plasmid into E. coli Transette (DE3) for prokaryotic expression after IPTG induction. The result showed that AhNAD-ME contains all of the motifs required for a complete and functional malic enzyme and is localized specifically to the mitochondrial matrix.Semi-quantitative RT-PCR results showed that AhNAD-ME was constitutively expressed in all examined tissues, with different expression levels, and strongly up-regulated under light in the leaf and stem. Results of SDS-PAGE demonstrated that the specific fusion protein with an expected molecular weight was successfully expressed in E. coli transette (DE3) induced by IPTG

Key words: Amaranthus hypochondriacus, AhNAD-ME, Sequence characteristics, Expression pattern, Prokaryotic expression

[1]Shearer H L, Turpin D H, Delinis D T. Characterization of NADP-dependent malic enzyme from developing castor oil seed endosperm. Arch Biochem Biophy, 2004, 429: 134–144



[2]Drincovich M F, Casati P, Andreo C S. NADP-malic enzyme from plants: a ubiquitous enzyme involved in different metabolic pathways. FEBS Lett, 2001, 490: 1–6



[3]Davies D D. The fine control of cytosolic pH. Physiol Plant, 1986, 67: 702–706



[4]董庆龙, 王海荣, 安淼, 余贤美, 王长君. 苹果NADP依赖的苹果酸酶基因克隆、序列和表达分析. 中国农业科学, 2013, 46: 1857–1866



Dong Q L, Wang H R, An M, Yu X M, Wang C J. Cloning, sequence and expression analysis of NADP-malic enzyme genes in apple. Sci Agric Sin, 2013, 46: 1857–1866 (in Chinese with English abstract)



[5]Casati P, Drincovich M F, Edwards G E, Andreo C S. Malate metabolism by NADP-malic enzyme in plant defense. Photosynth Res, 1999, 61: 99–105



[6]Fu Z Y, Zhang Z B, Hu X J, Shao H B, Ping X. Cloning, identification, expression analysis and phylogenetic relevance of two NADP-dependent malic enzyme genes from hexaploid wheat. Comptes Rendus Biol, 2009, 332: 591–602



[7]Berry J O, Yerramsetty P, Zielinski A M, Mure C M. Photosynthetic gene expression in higher plants. Photosynth Res, 2013, 117: 91–120



[8]Moreney J V, Jungnick N, Dimario R J, Longstreth D J. Photorespiration and carbon concentrating mechanisms: two adaptation to high O2, low CO2 conditions. Photosynth Res, 2013, 117: 121–131



[9]Langdale J A. C4 Cycles: Past, present, and future research on C4 photosynthesis. Plant Cell, 2011, 23: 3879–3892



[10]Tausta S, Coye H, Rothermel B, Stiefel V, Nelson T. Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor. Plant Mol Biol, 2002, 50: 635–652



[11]Detarsio E, Maurino V G, Alvarez C E, Mueller G L. Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots. Plant Mol Biol, 2008, 68: 355–367



[12]Maurino V, Drincovich M, Casati P, Andreo C, Edwards G, Ku M, Gupta S, Franceschi V. NADP-malic enzyme: immunolocalization in different tissues of the C4 plant maize and the C3 plant wheat. J Exp Bot, 1997, 48: 799–811



[13]Chi W, Yang J H, Wu N H, Zhang F. Four rice genes encoding NADP-malic enzyme exhibit distinct expression profiles. Bios Biotechnol Biochem, 2004, 68: 1865–1874



[14]Detarsio E, Andreo C S, Drincocich M F. Basic residues play key roles in catalysis and NADP+-specificity in maize (Zea mays L.) photosynthetic NADP+-dependent malic enzyme. Biochem J, 2004, 382: 1025–1030



[15]张锡州, 李廷轩, 王昌金. 富钾植物籽粒览研究进展. 中国农学通报, 2005, 21(4): 230–235



Zhang X Z, Li T X, Wang C J. Progress of research on K-rich plant of grain amaranth. Chin Agric Sci Bull, 2005, 21(4): 230–235 (in Chinese with English abstract)



[16]李廷轩, 马国瑞. 籽粒苋富钾基因型的根系形态和生理特性. 作物学报, 2004, 30: 1145–1151



Li T X, Ma G R. Physiological and morphological characteristics of roots in grain amaranth genotypes enrichment in potassium. Acta Agron Sin, 2004, 30: 1145–1151 (in Chinese with English abstract)



[17]Johnson B L, Henderson T L. Water use patterns of grain amaranth in the northern great plains. Agron J, 2002, 94: 1437–1443



[18]Shukla S, Bhargava A, Chatterjee A, Srivastava A, Singh S P. Genotypic variability in vegetable amaranth (Amaranthus tricolor L.) for foliage yield and its contributing traits over successive cuttings and years. Euphytica, 2006, 151: 103–110



[19]李平, 白云凤, 张维锋. 籽粒苋苹果酸酶基因克隆及分析. 西北植物学报, 2010, 30: 229–236



Li P, Bai Y F, Zhang W F. Cloning and analysis of NAD-ME gene of Amaranthus hypochondriacus. Acta Bot Boreali-Occident Sin, 2010, 30: 229–236 (in Chinese with English abstract)



[20]李平, 白云凤, 冯瑞云, 王原媛, 张维锋. 籽粒苋苹果酸酶(NAD-ME)基因密码子偏好性分析. 应用与环境生物学报, 2011, 17: 12–17



Li P, Bai Y F, Feng R Y, Wang Y Y, Zhang W F. Analysis of codon bias of NAD-ME gene in Amarnathus hypochondriacus. Chin J Appl Environ Biol, 2011, 17: 12–17 (in Chinese with English abstract)



[21]Petersen T, Brunak S, von Heijne G, Nielsen H. SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods, 2011, 8: 785–786



[22]Truscott K N, Brandner K, Pfanner N. Mechanisms of Protein Import into Mitochondria. Curr Biol, 2003, 13: R326–R337



[23]von Heijne G, Steppuhn J, Herrmann R G. Domain structure of mitochondrial and chloroplast targeting peptides. EurJ Biochem, 1989, 180: 535–545



[24]Christopher J, Tonkin C J, Foth B J, Ralph S A, Struck N, Cowman A F, McFadden G I. Evolution of malaria parasite plastid targeting sequences. Proc Natl Acad Sci USA, 2008, 105: 4781–4785



[25]Long J J, Wang J L, Berry J O. Cloning and analysis of the C4 photosynthetic NAD-dependeng malic enzyme of Amaranth Mitochondria. J Biol Chem, 1994, 269: 2827–2833



[26]Murata T, Ohsugi R, Matsuoka M, Nakamoto H. Purification and characterization of NAD malic enzyme from Leaves of Eleusine coracana and Panicum dichotomiflorum. Plant Physiol, 1989, 89: 316–324



[27]Oshugi R, Murata T. Leaf anatomy, post-illumination CO2 burst and NAD-malic enzyme activity in Panicum dichotomiflorum. Plant Cell Physiol, 1980, 21: 1329–1333



[28]Esposito D, Chatterjee D K. Enhancement of soluble protein expression through the use of fusion tags. Curr Opin Biotechnol, 2006, 17: 353–358



[29]Waugh D S. Making the most of affinity tags. Trends Biotechnol, 2005, 23: 316–320

[1] Sun Shu-Feng, Xu Zhen-Nan, Huang Jia-Xin, Weng Jian-Feng, Li Xin-Hai. Genome-wide identification of the maize MAPK gene family and its response to Fusarium verticillioides infection [J]. Acta Agronomica Sinica, 2026, 52(5): 1291-1308.
[2] Hu Cheng-Zhen, Gao Wei-Dong, Kong Bin-Xue, Wang Jian-Fei, Che Zhuo, Yang De-Long, Chen Tao. Genome-wide identification of the TaAPC11 gene family in wheat and functional characterization of TaAPC11-5B in drought stress responses [J]. Acta Agronomica Sinica, 2026, 52(1): 148-164.
[3] YAN Zhi-Lan, ZHAO Qin, CHANG Tian-Da, WANG Yi-Ming, WANG Bi-Hui, WANG Peng, HUANG Chun-Guo, ZHANG Hui, WANG Li-Xiang, HAO Xiao-Peng, ZHAO Bo. Genome-wide identification and characterization of Alternative oxidase (AOX) genes in leguminous crops and their expression patterns in response to abiotic stresses in common bean [J]. Acta Agronomica Sinica, 2025, 51(7): 1769-1783.
[4] SHEN Ao, LIU Min, NI Di-An, LIU Wei. Promoter characterization and expression pattern analysis of the m6A methyltransferase gene SiMTA1 in foxtail millet [J]. Acta Agronomica Sinica, 2025, 51(7): 1969-1978.
[5] XU Lin-Shan, GAO Geng-Dong, WANG Yu, WANG Jia-Xing, YANG Ji-Zhao, WU Ya-Rui, ZHANG Xiao-Han, CHANG Ying, LI Zhen, XIE Xiong-Ze, GONG De-Ping, WANG Jing, GE Xian-Hong. Analysis of expression patterns of laccase gene family members in Brassica napus and their association with stem fracture resistance [J]. Acta Agronomica Sinica, 2025, 51(1): 134-148.
[6] LIU Zhen, CHEN Li-Min, LI Zhi-Tao, ZHU Jin-Yong, WANG Wei-Lu, QI Zhe-Ying, YAO Pan-Feng, BI Zhen-Zhen, SUN Chao, BAI Jiang-Ping, LIU Yu-Hui. Genome-wide identification and expression analysis of ARM gene family in potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2024, 50(6): 1451-1466.
[7] ZUO Chun-Yang, LI Ya-Wei, LI Yan-Long, JIN Shuang-Xia, ZHU Long-Fu, ZHANG Xian-Long, MIN Ling. Relative expression patterns of laccase gene family members in upland Gossypium hirsutum L. [J]. Acta Agronomica Sinica, 2023, 49(9): 2344-2361.
[8] MA Chun-Min, LI Wei-Xi, LI Fang-Jun, TIAN Xiao-Li, LI Zhao-Hu. Identification and expression analysis of nitrate transporter NRT gene family in upland cotton (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2023, 49(6): 1496-1517.
[9] XU Zi-Yin, YU Xiao-Ling, ZOU Liang-Ping, ZHAO Ping-Juan, LI Wen-Bin, GENG Meng-Ting, RUAN Meng-Bin. Expression pattern analysis and interaction protein screening of cassava MYB transcription factor MeMYB60 [J]. Acta Agronomica Sinica, 2023, 49(4): 955-965.
[10] SUN Quan-Xi, YUAN Cui-Ling, MOU Yi-Fei, YAN Cai-Xia, ZHAO Xiao-Bo, WANG Juan, WANG Qi, SUN Hui, LI Chun-Juan, SHAN Shi-Hua. Genome-wide identification and expression analysis of SWEET genes from peanut genomes [J]. Acta Agronomica Sinica, 2023, 49(4): 938-954.
[11] YANG Jia-Bao, ZHANG Zhan, ZHOU Zhi-Ming, LYU Xin-Hua, SUN Li. Cloning and function analysis of a HaLACS9 gene in Helianthus annuus L. [J]. Acta Agronomica Sinica, 2023, 49(2): 426-437.
[12] ZHAO Li-Rong, LI Wen, WANG Li-Min, QI Yan-Ni, LI Wen-Juan, XIE Ya-Ping, DANG Zhao, ZHAO Wei, ZHANG Jian-Ping. Identification and relative expression pattern of PLA1 gene family in flax [J]. Acta Agronomica Sinica, 2023, 49(11): 2949-3295.
[13] CHEN Wu-Jun, LIU Jiang-Dong, JIANG Kai-Xuan, WANG You-Ping, JIANG Jin-Jin. Identification and analysis of BnKNOX gene family in Brassica napus [J]. Acta Agronomica Sinica, 2023, 49(11): 2991-3006.
[14] SUN Lan-Lan, MA Rong-Hui, XUE Fei, YANG Mu-Han, XU Hong-Le, SU Wang-Cang, LU Chuan-Tao, WU Ren-Hai. Cloning and the relative expression pattern of GST31 gene in maize [J]. Acta Agronomica Sinica, 2023, 49(10): 2717-2726.
[15] WANG Heng-Bo, ZHANG Chang, WU Ming-Xing, LI Xiang, JIANG Zhong-Li, LIN Rong-Xiao, GUO Jin-Long, QUE You-Xiong. Genome-wide identification of NAC transcription factors ATAF subfamily in Sacchrum spontaneum and functional analysis of its homologous gene ScNAC2 in sugarcane cultivar [J]. Acta Agronomica Sinica, 2023, 49(1): 46-61.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!