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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (01): 145-153.doi: 10.3724/SP.J.1006.2015.00145


Drought Resistance Identification of Mungbean Germplasm Resources at Seedlings Stage

WANG Lan-Fen,WU Jing,JING Rui-Lian,CHENG Xu-Zhen,WANG Shu-Min?   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China?
  • Received:2014-05-22 Revised:2014-09-16 Online:2015-01-12 Published:2014-09-26
  • Contact: 王述民, E-mail: wangshumin@caas.cn


It is significant to identify and screen drought resistant mungbean germplasm resources at seedling stage for mungbean cultivar improvement and production in China, at the same time, which could provide some reference methods and basic information for developing “the technical specification of identification and evaluation for drought resistance in mungbean”. The indices including survival rate of seedlings, wilt index, plant height, weight of fresh and dry leaf, relative water content, biomass weight and stress index were measured with the method of repeat drought stress in 70 accessions of mungbean resources. The survival rate of seedlings had significantly positive correlation between the first and the second drought stress, both wilt index and plant height had significantly negative correlation with survival rate. The survival rate of seedling under the first drought stress, wilt index and plant height were selected as suitable indices for drought resistance identification and evaluation based on correlation analysis. The various resistant accessions of mungbean germplasm resources screened by subordinative function analysis included 16 highly resistant, 20 resistant, 23 moderately resistant, eight susceptible and three highly susceptible. The drought resistance was evaluated based on the survival rate of seedling under the first drought stress, wilt index and plant height with the concordance rate of 70.0%, 58.6%, and 51.4% respectively as compared to the comprehensive evaluation. The survival rate of seedling under the first drought stress was recommended as a drought resistance index in screening a large number of germplasm resources ofmungbean.

Key words: Mungbean, Seedling stage, Repeat drought, Drought resistance, Correlation analysis, Subordinative function analysis

[1]2013年中国绿豆生产业发展迅速. http://www.ibaogao.com/free/03221102T2013.html [Issued date: 2013-3-22] [Visited date: 2014-3-14]

[2]张璞, 田建华. 抗旱性绿豆种质的选育. 干旱地区农业研究, 1999,17(4): 41–44

Zhang P, Tian J H. Drought resistance breeding of mungbean germplasm resources. Agric Res Arid Areas, 1999, (4): 41–44 (in Chinese)

[3]景蕊莲. 作物抗旱研究的现状与思考. 干旱地区农业研究, 1999, 17(2):79–85

Jing R L. The statues and deliberate of research drought on crops. Agric Res Arid Areas, 1999, 17(2): 79–85 (in Chinese)

[4]殷桂香, 王瑾, 徐惠君, 陶丽莉, 杜丽璞, 毛新国, 田小海, 叶兴国. 几个小麦基因型苗期抗旱性鉴定及相关生理指标分析. 麦类作物学报, 2009, 29: 319–323

Yin G X, Wang J, Xu H J, Tao L L, Du L P, Mao X G, Tian X H, Ye X G. Drought tolerance test and related seedling stage of physiological indexes analysis several wheat genotypes. J Triticeae Crops, 2009, 29: 319–323 (in Chinese with English abstract)

[5]高吉寅, 胡荣海, 路漳, 杨国良. 水稻等种质苗期抗早生理指标的探讨. 中国农业科学, 1984, 17(4): 41–46

Gao J Y, Hu R H, Lu Z, Yang G L. Exploring physiological indexes of drought resistance during seedling in rice resources. Sci Agric Sin, 1984, 17(4): 41–46 (in Chinese)

[6]王贺正, 李艳, 马均, 张荣萍, 李旭毅.水稻苗期抗旱性指标的筛选. 作物学报, 2007, 33: 1523–1529

Wang H Z, Li Y, Ma J, Zhang R P, Li X Y. Screening indexes of drought resistance during seedling in rice. Acta Agron Sin, 2007, 33: 1523–1529 (in Chinese with English abstract)

[7]杜家会, 董超, 汤翠凤, 徐福荣, 张斐斐, 杨雅云, 张恩来, 阿新祥, 戴陆园. 缅甸引进稻种资源苗期抗旱性鉴定. 浙江农业学报, 2012, 24: 764–770

Du J H, Dong C, Tang C F, Xu F R, Zhang F F, Yang Y Y, Zhang E L, A X X, Dai L Y. Evaluation drought resistance on the rice germplasms introducted from Myanmar at the seedling stage. Acta Agric Zhejiangensis, 2012, 24: 764–770 (in Chinese with English abstract)

[8]武斌, 李新海, 肖木辑, 谢传晓, 郝转芳, 李明顺, 张世煌. 53份玉米自交系的苗期耐旱性分析. 中国农业科学, 2007, 40:665–676

Wu B, Li X H, Xiao M J, Xie C X, Hao Z F, Li M S, Zhang S H. Genetic variation in fifty-three maize inbred lines in relation to drought tolerance at seedling stage. Sci Agric Sin, 2007, 40: 665–676 (in Chinese with English abstract)

[9]冯朋飞, 远红杰, 郭晋杰.不同玉米自交系苗期抗旱性鉴定. 广东农业科学, 2013,(9):9–13

Feng P F, Yuan H J, Guo J J. Evaluation for seeding drought resistance of different genotype maize inbred lines. Sci Agric Guangdong, 2013, (9): 9–13 (in Chinese with English abstract)

[10]汪军成, 孟亚雄, 徐先良, 王晋, 赖勇, 李葆春, 马小乐, 王化俊. 大麦苗期抗旱性鉴定及评价. 干旱地区农业研究, 2013, 31(4): 135–143

Wang J C, Meng Y X, Xu X L, Wang J, Lai Y, Li B C, Ma X L, Wang H J. Identification and assessment on drought-resistance of Hordeum vulgare L. at seedling stage. Agric Res Arid Areas, 2013, 31(4): 135–143

[11]农业部科技司. 中国农业科技研究进展(第1分册), 北京:农业出版社, 1991. pp 54–56

Ministry of Agriculture Science and Technology Division. Advances in Agricultural Science and Technology Research Chinese (Part 1). Beijing: China Agriculture Press, 1991. pp 54–56 (in Chinese)

[12]王育红, 姚宇卿, 张灿军, 吕军杰, 张洁, 王聪慧, 李俊红. 旱稻抗旱性鉴定方法与指标研究: IV. 旱稻苗期抗旱性. 干旱地区农业研究, 2005, 23(4): 134–137

Wang YH, Yao YQ, Zhang C J, Lü J J, Zhang J, Wang C H, Li J H. Study on resistance drought identify method and index of upland rice: IV:  Resistance drought of upland rice in seedling. Agric Res Arid Areas, 2005, 23(4): 134–137 (in Chinese)

[13]杨子光, 张灿军, 冀天会, 郭军伟, 孟丽梅, 张珂.小麦抗旱性鉴定方法及评价指标研究: V. 苗期抗旱指标的比较研究. 中国农学通报, 2008, 24(1):156–159

Yang Z G, Zhang C J, Ji T H, Guo J W, Meng L M, Zhang K.Study on resistance drought identify method and evaluation index of wheat V the comparative studyon resistance drought index of wheat in seedling. Chin Agric Sci Bull, 2008, 24(1): 156–159 (in Chinese with English abstract)

[14]Todd G W, Webster D L. Effect of repeated drought periods on photosynthesis and survival of cereal seedlings. J Agron, 1965, 57: 399–404

[15]胡荣海, 昌小平, 王环. 反复干旱法的生理基础及其应用. 华北农学报, 1996, 11(3): 51–56

Hu R H, Chang X P, Wang H. The physiological base and utilization of repeated drought method. Acta Agric Boreali-Sin, 1996, 11(3): 51–56 (in Chinese with English abstract)

[16]Bouslama M, Schapaugh W T. Stress tolerance in soybeans: I. Evaluation of three screening techniques for heat and drought tolerance. Crop Sci, 1984, 24: 933–937

[17]王兰芬, 武晶, 景蕊莲, 程须珍, 王述民. 绿豆种质资源芽期抗旱性鉴定. 植物遗传资源学报, 2014, 15: 498–503

Wang L F, Wu J, Jing R L, Cheng X Z, Wang S M. drought resistance identification of mungbean germplasm resources at germination stage. J Plant Genet Resour, 2014, 15:498–503 (in Chinese with English abstract)

[18]景蕊莲, 胡荣海, 张灿军, 朱志华, 昌小平, 王娟玲.小麦抗旱性鉴定评价技术规范. 北京:中国标准出版社, 2008. pp1–5

Jing R L, Hu R H, Zhang C J, Zhu Z H, Chang X P, Wang J L. Technical Specification of Identification and Evaluation for Drought Resistance in Wheat. GB/T 21127-2007. Beijing: China Standards Press, 2008. pp 1–5 (in Chinese)

[19]姚艳荣, 贾秀领, 马瑞昆, 贾银锁, 张丽华, 董志强, 申海平, 郭丽.一种新型玉米苗期反复干旱存活率鉴定试验设计及效果研究.华北农学报, 2010,25(增刊):148–151

Yao Y R, Jia X L, Ma R K, Jia Y S, Zhang L H, Dong Z Q, Shen H P, Guo L. A new design method of double randomized block with single planting for evaluating seedling survival percentage after a repeated drought stress in maize seedling survival .Acta Agric Boreali-Sin, 2010,25(suppl):148–151(in Chinese with English abstract)

[20]龚明. 作物抗旱性鉴定方法与指标及其综合评价. 云南农业大学学报, 1989, 4: 73–81

Gong M. The methods and indices of identification drought resistance and comprehensive evaluation. J Yunnan Agric Univ, 1989, 4:73–81(in Chinese)

[21]黎裕. 作物抗旱性鉴定方法与指标. 干旱地区农业研究, 1993, 11(1):91–99

Li Y. The methods and indices of identification drought resistance on crops. Agric Res Arid Areas, 1993, 11(1): 91–99(in Chinese)

[22]蒲伟凤, 纪展波, 李桂兰, 乔亚科.作物抗旱性鉴定方法研究进展. 河北科技师范学院学报, 2011, 25(2): 34–39

Pu W F, Ji Z B, Li G L, Qiao Y K. The research progress of identification drought resistance methods. J Hebei Norm Univ Sci&Technol, 2011, 25(2): 34–39 (in Chinese with English abstract)

[23]敬礼恒, 刘利成, 梅坤, 陈光辉. 水稻抗旱性能鉴定方法及评价指标研究进展. 中国农学通报, 2013, 29(12): 1–5

Jing L L, Liu L C, Mei K, Chen G H. Research progress of drought resistance identification and evaluation in rice. Chin Agric Sci Bull, 2013, 29(12): 1–5 (in Chinese with English abstract)

[24]胡荣海. 农作物抗早鉴定方法和指标. 作物种质资源, 1986, (4): 36–39

Hu R L.The methods and indices drought resistance identification in crops. Crops Germplasm Resour, 1986, (4): 36–39 (in Chinese with English abstract)

[25]白玉. 谷子萌发期和苗期抗旱性研究及抗旱鉴定指标的筛选. 首都师范大学硕士论文, 北京, 2009. pp 10–47

Bai Y. Researching and Screening the Indices of Identification Drought Resistance at Germination and Seedling in Foxtail Millet Originated from China. MS Thesis of Capital Normal University, Beijing, China, 2009. pp 10–47

[26]邱鹏程, 张闻博, 李灿东, 蒋洪蔚, 刘春燕, 范冬梅, 曾庆力, 胡国华, 陈庆山. 利用选择导入系分析大豆芽期和苗期耐旱性的遗传重叠. 作物学报, 2011, 37: 477−483

Qiu P C, Zhang W B, Li C D, Jiang H W, Liu C Y, Fan D M, Zeng Q L, Hu G H, Chen Q S. Genetic overlap of drought-tolerance loci between germination stage and seedling stage analyzed using introgression lines in soybean. Acta Agron Sin, 2011, 37: 477−483 (in Chinese with English abstract)

[27]姜奇彦, 胡正, 张辉, 王萌萌, 唐俊源, 倪志勇, 姜锋. 大豆种质资源耐盐性鉴定与研究. 植物遗传资源学报, 2012, 13: 726–732

Jiang Q Y, Hu Z, Zhang H, Wang M M, Tang J Y, Ni Z Y, Jiang F. Evaluation for salt tolerance in soybean cultivars (Glycine max L. Merrill). J Plant Genet Resour, 2012, 13: 726–732 (in Chinese with English abstract)

[28]刘鸿艳, 邹桂花, 刘国兰, 胡颂平, 李明寿, 余新桥, 梅捍卫, 罗利军. 水分梯度下水稻CT、LWP和SF的相关及其QTL定位研究. 科学通报, 2005, 50: 130–139

Liu H Y, Zou G L, Liu G L, Hu S P, Li M S, Yu X Q, Mei H W, Luo L J. Studies on relationships between CT, LWP and SF and their QTL mapping in rice. Sci Bull, 2005, 50: 130–139 (in Chinese)

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