Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (03): 386-393.doi: 10.3724/SP.J.1006.2015.00386

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Bacterial Blight Resistance Gene in Yunnan Wild Rice

LI Ding-Qin,CHEN Ling,LI Wei-Jiao,KE Xue,YU Teng-Qiong,LI E-Xian,HUANG Xing-Qi,CHENG Zai-Quan*   

  1. 1Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences / Yunnan Provincial Key Laboratory of Agricultural Biotechnology / Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223, China
  • Received:2014-09-23 Revised:2014-12-19 Online:2015-03-12 Published:2015-01-12
  • Contact: 程在全,E-mail: czquan-99@163.com E-mail:lidingqin37@aliyun.com

Abstract:

Bacterial blight (BB) is one of the most destructive diseases in rice production. The most efficient and economical way of controlling the disease is developing disease resistance varieties. Owing to the limited number of resistance genes from cultivated rice and the narrow spectrum of some bacterial blight resistance genes, exploring new resistance genes from wild rice will be conducive to development of new resistance varieties with wider spectrum and higher resistance. Yunnan is rich in resources of wild rice. However, till now there are few reports concerning whether they contain seven cloned BB resistance genes. In this study, seven cloned BB genes were detected in Yunnan wild rice by resistance identification and PCR analysis. The results showed that Yunnan wild rice had different levels of resistance to BB strains Y8 and PXO99, and O. granulata was immune to Y8 and PXO99. The resistance genes xa5, xa13 and Xa21 were not found in all three wild rice materials. Xa1 was detected in O. rufipogon type from Jinghong. Xa3/Xa26 was identified in O. rufipogon types from Yuanjiang and Jinghong, respectively. Xa3/Xa26 was alsoidentified in O. officinalis, Xa23 was found in O. rufipogon type from Yuanjiang, Xa27 in O. rufipogon type from Jinghong, and Xa27 in O. granulata. The results provided a theoretical reference for further exploring and cloning new BB resistance genes in Yunnan wild rice to improve the disease resistance of cultivated rice.

Key words: Wild rice, Bacterial blight, Resistance gene, PCR identification

[1]Mew T W. Current status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol, 1987, 25: 359–382



[2]Song W, Wang G, Chen L, Kim H S, Pi L Y, Holsten T, Gardner J, Wang B, Zhai W X, Zhu L H, Fauquet C, Ronald P. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 1995, 270: 1804–1806



[3]Yoshimura S, Yamanouchi U, Katayose Y, Toki S, Wang Z X, Kono I, Kurata N, Yano M, Iwata N, Sasaki T. Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation. Proc Natl Acad Sci USA, 1998, 95: 1663–1668



[4]Xiang Y, Cao Y L, Xu C G, Li X H, Wang S P. Xa3, conferring resistance for rice bacterial blight and encoding a receptor kinase-like protein, is the same as Xa26. Theor Appl Genet, 2006, 113: 1347–1355



[5]Iyer A S, McCouch S R. The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Mol Plant Microbe Int, 2004, 17: 1348–1354



[6]Jiang G H, Xia Z H, Zhou Y L, Wan J, Li D Y, Chen R S, Zhai W X, Zhu L H. Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5(Xa5) in comparison with its homolog TFIIAγ1. Mol Gen Genomics, 2006, 275: 354–366



[7]Gu K Y, Yang B, Tian D, Wu L, Wang D, Sreekala C, Yang F, Chu Z, Wang G L, White F F, Yin Z. R gene expression induced by a type-III effector triggers disease resistance in rice. Nature, 2005, 435: 1122–1125



[8]Chu Z H, Yuan M, Yao J L, Ge X J, Yuan B, Xu C G, Li X H, Fu B Y, Li Z K, Bennetzen J L, Zhang Q F, Wang S P. Promoter mutations of an essential gene for pollen development result in disease resistance in rice. Genes Dev, 2006, 20: 1–5



[9]王春连. 水稻抗白叶枯病基因Xa23的图位克隆. 中国农业科学院博士学位论文, 北京, 2006



Wang C L. Map-based Cloning of Rice Bacterial Blight Resistance Gene Xa23. Ph.D. Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2006 (in Chinese with English abstract)



[10]郭嗣斌, 张端品, 林兴华. 小粒野生稻抗白叶枯病新基因的鉴定与初步定位. 中国农业科学, 2010, 43: 2611–2618



Guo S B, Zhang D P, Lin X H. Identification and mapping of a novel bacterial blight resistance gene Xa35(t) originated from Oryza minuta. Sci Agric Sin, 2010, 43: 2611–2618 (in Chinese with English abstract)



[11]夏春, 陈红旗, 朱旭东. 水稻白叶枯病抗性基因的鉴定、定位、克隆与育种应用. 分子植物育种, 2012, 10: 761–771



Xia C, Chen H Q, Zhu X D. Identification, mapping, isolation of the genes resisting to bacterial blight and their breeding application in rice. Mol Plant Breed, 2012, 10: 761–771 (in Chinese with English abstract)



[12]鄂志国, 王磊. 野生稻有利基因的发掘和利用. 遗传, 2008, 30: 1397–1405



E Z G, Wang L. Discovery and utilization of favorable genes in wild rice. Hereditas (Beijing), 2008, 30: 1397–1405 (in Chinese with English abstract)



[13]谭光轩, 任翔, 翁清妹, 时振英, 祝莉莉, 何光存. 药用野生稻转育后代一个抗白叶枯病新基因的定位. 遗传学报, 2004, 31: 724–729



Tan G X, Ren X, Weng Q M, Shi Z Y, Zhu L L, He G C. Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Acta Genet Sin, 2004, 31: 724–729 (in Chinese with English abstract)



[14]金旭炜, 王春连, 杨清, 江祺祥, 樊颖伦, 刘古春, 赵开军. 水稻抗白叶枯病近等基因系CBB30的培育及Xa30(t)的初步定位. 中国农业科学, 2007, 40(6): 1094–1100



Jin X W, Wang C L, Yang Q, Jiang Q X, Fan Y L, Liu G C, Zhao K J. Breeding of near-isogenic line CBB30 and molecular mapping of Xa30(t), a new resistance gene to bacterial blight in rice. Sci Agric Sin, 2007, 40: 1094–1100 (in Chinese with English abstract)



[15]郑崇珂, 王春连, 于元杰, 梁云涛, 赵开军. 水稻抗白叶枯病新基因Xa32(t)的鉴定和初步定位. 作物学报, 2009, 35: 1173–1180



Zheng C K, Wang C L, Yu Y J, Liang Y T, Zhao K J. Identification and molecular mapping of Xa32(t), a novel resistance gene for bacterial blight (Xanthomonas oryzae pv. oryzae) in rice. Acta Agron Sin, 2009, 35: 1173–1180 (in Chinese with English abstract)



[16]Ruan H H, Yan C Q, An D R, Liu R H, Chen J P. Identifying and mapping new gene xa32(t) for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae, Xoo) from Oryza meyeriana L. Acta Agric Boreali-Occident Sin, 2008, 17(6): 170–174



[17]Cheng Z Q, Huang X Q, Zhang Y Z, Qian J, Yang M Z, Wu C J, Liu J F. Diversity in the content of some nutritional components in husked seeds of three wild rice species and rice varieties in Yunnan province of China. J Integr Plant Biol, 2005, 47: 1260–1270



[18]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4325



[19]范宏环, 王林友, 张礼霞, 于新, 王曦, 金庆生, 王建军. 通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系. 中国水稻科学, 2011, 25: 331–334



Fan H H, Wang L Y, Zhang L X, Yu X, Wang X, Jin Q S, Wang J J. Breeding of rice lines with bacterial blight resistance gene Xa23 by using marker-assisted selection. Chin J Rice Sci, 2011, 25: 331–334 (in Chinese with English abstract)



[20]Xia Z H, Han F, Gao L F, Yuan Q H, Zhai W X, Liu D, Luo Y H. Application of functional markers to identify genes for bacterial blight resistance in Oryza rufipogon. Rice Sci, 2010, 17: 73–76



[21]彭绍裘, 魏子生, 毛昌祥, 黄河清, 肖放华, 罗宽. 云南省疣粒野生稻、药用野生稻和普通野生稻多抗性鉴定. 植物病理学报, 1982, 12(4): 58–60



Peng S Q, Wei Z S, Mao C X, Huang H Q, Xiao F H, Luo K. Identification of multiresistance of O. meyeriana, O. officinalis and O. sativa f. spantanea growing in Yunnan Province. Acta Phytopathol Sin, 1982, 12(4): 58–60 (in Chinese)



[22]Cheng Z Q, Ying F Y, Li D Q, Yu T Q, Fu J, Yan H J, Zhong Q F, Zhang D Y, Li W J, Huang X Q. Genetic diversity of wild rice species in Yunnan Province of China. Rice Sci, 2012, 19(1): 21–28



[23]钱君, 程在全, 杨明挚, 刘继梅, 吴成军, 黄兴奇. 云南野生稻中Xa21基因外显子II的分离及序列分析. 遗传, 2005, 27: 382–386



Qian J, Cheng Z Q, Yang M Z, Liu J M, Wu C J, Huang X Q. Isolation and sequence analysis of the Xa21 gene exon II homologs from different species of wild rice in Yunnan. Hereditas (Beijing), 2005, 27: 382–386 (in Chinese with English abstract)



[24]程在全, 晏慧君, 耿显胜, 殷富有, 孙一丁, 章成, 黄兴奇. 云南疣粒野生稻抗白叶枯病鉴定及叶片组织学观察. 植物病理学报, 2008, 38: 582–591



Cheng Z Q, Yan H J, Geng X S, Yin F Y, Sun Y D, Zhang C, Huang X Q. Identification of Oryza granulata for resistance to Xanthomonas oryzae pv. oryzae and observation of leaf tissue. Acta Phytopathol Sin, 2008, 38: 582–591 (in Chinese with English abstract)



[25]杨青, 王春台, 刘学群, 谭艳平. 水稻白叶枯病抗性基因Xa31(t)候选基因的克隆及遗传转化. 中国农学通报, 2011, 27(18): 223–227



Yang Q, Wang C T, Liu X Q, Tan Y P. Cloning and genetic transformation of the candidate genes for the bacterial blight resistance gene Xa31(t) in rice. Chin Agric Sci Bull, 2011, 27(18): 223–227 (in Chinese with English abstract)

[1] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[2] Su Ai-Guo, Xiao Sen-Lin, Yi Hong-Mei, Duan Sai-Ru, Wang Shuai-Shuai, Zhang Ru-Yang, Xing Jin-Feng, Li Chun-Hui, Sun Xuan, Xu Rui-Bin, Xu Tian-Jun, Li Zhi-Yong, Zhang Yong, Wang Rong-Huan, Song Wei, Zhao Jiu-Ran. Research progress and breeding application of resistance genetics to ear rot in maize [J]. Acta Agronomica Sinica, 2026, 52(1): 1-13.
[3] Dong Li-Hua, Dong Cheng-Yan, Li Zheng-Nan, Yu Jing, Ye Liang, Liu Fang, Tan Jing. Screening and identification of candidate resistance genes to gibberella ear rot caused by Fusarium graminearum in maize [J]. Acta Agronomica Sinica, 2026, 52(1): 131-147.
[4] ZHANG Zheng-Kang, SU Yan-Hong, RUAN Sun-Mei, ZHANG Min, ZHANG Pan, ZHANG Hui, ZENG Qian-Chun, LUO Qiong. Cloning and functional study of OgXa13 in Oryza meyeriana [J]. Acta Agronomica Sinica, 2025, 51(2): 334-346.
[5] XIE Liu-Jie, DUAN Min, YANG Yong, PAN Xiao-Biao, MA Bo-Jun, HUANG Shan-Jun, CHEN Xi-Feng. Development of wide-compatibility restorer lines resistant to bacterial blight and their application in rice hybrid breeding [J]. Acta Agronomica Sinica, 2025, 51(12): 3133-3143.
[6] HUANG Lin-Yu, ZHANG Xiao-Yue, LI Hao, DENG Mei, KANG Hou-Yang, WEI Yu-Ming, WANG Ji-Rui, JIANG Yun-Feng, CHEN Guo-Yue. Mapping of QTL for adult plant stripe rust resistance genes in a Sichuan wheat landrace and the evaluation of their breeding effects [J]. Acta Agronomica Sinica, 2024, 50(9): 2167-2178.
[7] LI Yu-Jia, XU Hao, YU Shi-Nan, TANG Jian-Wei, LI Qiao-Yun, GAO Yan, ZHENG Ji-Zhou, DONG Chun-Hao, YUAN Yu-Hao, ZHENG Tian-Cun, YIN Gui-Hong. Genetic analysis of elite stripe rust resistance genes of founder parent Zhou 8425B in its derived varieties [J]. Acta Agronomica Sinica, 2024, 50(1): 16-31.
[8] XUE Jiao, LU Dong-Bai, LIU Wei, LU Zhan-Hua, WANG Shi-Guang, WANG Xiao-Fei, FANG Zhi-Qiang, HE Xiu-Ying. Genetic analysis and fine mapping of a bacterial blight resistance major QTL qBB-11-1 in high-quality rice ‘Yuenong Simiao’ [J]. Acta Agronomica Sinica, 2022, 48(9): 2210-2220.
[9] ZHANG Yi-Duo, LI Guo-Qiang, KONG Zhong-Xin, WANG Yu-Quan, LI Xiao-Li, RU Zhen-Gang, JIA Hai-Yan, MA Zheng-Qiang. Breeding of FHB-resistant wheat line Bainong 4299 by gene pyramiding [J]. Acta Agronomica Sinica, 2022, 48(9): 2221-2227.
[10] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[11] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[12] ZHAO Hai-Han, LIAN Wang-Min, ZHAN Xiao-Deng, XU Hai-Ming, ZHANG Ying-Xin, CHENG Shi-Hua, LOU Xiang-Yang, CAO Li-Yong, HONG Yong-Bo. Genetic dissection of the bacterial blight disease resistance in super hybrid rice RILs using genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(1): 121-137.
[13] LUO Lan, LEI Li-Xia, LIU Jin, ZHANG Rui-Hua, JIN Gui-Xiu, CUI Di, LI Mao-Mao, MA Xiao-Ding, ZHAO Zheng-Wu, HAN Long-Zhi. Mapping QTLs for yield-related traits using chromosome segment substitution lines of Dongxiang common wild rice (Oryza rufipogon Griff.) and Nipponbare (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2021, 47(7): 1391-1401.
[14] JIANG Wei, PAN Zhe-Chao, BAO Li-Xian, ZHOU Fu-Xian, LI Yan-Shan, SUI Qi-Jun, LI Xian-Ping. Genome-wide association analysis for late blight resistance of potato resources [J]. Acta Agronomica Sinica, 2021, 47(2): 245-261.
[15] ZHANG Rong-Yue, WANG Xiao-Yan, YANG Kun, SHAN Hong-Li, CANG Xiao-Yan, LI Jie, WANG Chang-Mi, YIN Jiong, LUO Zhi-Ming, LI Wen-Feng, HUANG Ying-Kun. Identification of brown rust resistance and molecular detection of Bru1 gene in new and main cultivated sugarcane varieties [J]. Acta Agronomica Sinica, 2021, 47(2): 376-382.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!