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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1147-1154.doi: 10.3724/SP.J.1006.2015.01147

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

EST-SSR and SNP Markers Based Barley Malt Purity Detection

ZHANG Li-Sha1,2,DONG Guo-Qing1,*,ZHA Sang2,3,ZHUO Ga3,WANG De-Liang4,GU Fang-Hong4,YUAN Xing-Miao2,ZHANG Jing2,GUO Gang-Gang2,*   

  1. 1 School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2 Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3Agricultural and Animal Husbandry College of Tibet University, Linzhi 860000, China; 4 China National Research Institute of Food and Fermentation Industries, Beijing 100027, China
  • Received:2014-12-15 Revised:2015-04-02 Online:2015-08-12 Published:2015-05-18
  • Contact: 董国清, E-mail: dongbio@126.com; 郭刚刚, E-mail: guoganggang@caas.cn E-mail:zhanglisa06@163.com

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Abstract:

Barley malt is one of the main raw materials used in beer production. Malt purity determines the level of its homogeneity, which affecting processing techniques and beer quality. For the efficient and accurate identification of malt purity, providing evidence for the malt raw materials procurement and quality monitoring in beer production, the purities of six proportionally premixed malt samples and another four blind samples were detected by using EST-SSR and SNP markers in this study. The results showed that the samples with mixing ratio higher than 10% were distinguished with EST-SSR markers in qualitative detection, whereas those with SNP markers were more effective even the sample impurity as low as 5%. In the quantitative detection, the standard error between the measured value and the true value was lower than 3% in one sampling. Obviously, both of the two types of molecular markers are all can be used for malt purity test, but KASP assay based-SNP detection is more suitable for rapid and quantitative malt purity test.

Key words: Barley malt, Purity test, Molecular marker, KASP

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