Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1147-1154.doi: 10.3724/SP.J.1006.2015.01147

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

EST-SSR and SNP Markers Based Barley Malt Purity Detection

ZHANG Li-Sha1,2,DONG Guo-Qing1,*,ZHA Sang2,3,ZHUO Ga3,WANG De-Liang4,GU Fang-Hong4,YUAN Xing-Miao2,ZHANG Jing2,GUO Gang-Gang2,*   

  1. 1 School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2 Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3Agricultural and Animal Husbandry College of Tibet University, Linzhi 860000, China; 4 China National Research Institute of Food and Fermentation Industries, Beijing 100027, China
  • Received:2014-12-15 Revised:2015-04-02 Online:2015-08-12 Published:2015-05-18
  • Contact: 董国清, E-mail: dongbio@126.com; 郭刚刚, E-mail: guoganggang@caas.cn E-mail:zhanglisa06@163.com

Abstract:

Barley malt is one of the main raw materials used in beer production. Malt purity determines the level of its homogeneity, which affecting processing techniques and beer quality. For the efficient and accurate identification of malt purity, providing evidence for the malt raw materials procurement and quality monitoring in beer production, the purities of six proportionally premixed malt samples and another four blind samples were detected by using EST-SSR and SNP markers in this study. The results showed that the samples with mixing ratio higher than 10% were distinguished with EST-SSR markers in qualitative detection, whereas those with SNP markers were more effective even the sample impurity as low as 5%. In the quantitative detection, the standard error between the measured value and the true value was lower than 3% in one sampling. Obviously, both of the two types of molecular markers are all can be used for malt purity test, but KASP assay based-SNP detection is more suitable for rapid and quantitative malt purity test.

Key words: Barley malt, Purity test, Molecular marker, KASP

[1]国家统计局. 中国统计年鉴: 2013. 北京: 中国统计出版社, 2014



National Bureau of Statistics of the People’s Republic of China. China Statistical Yearbook: 2013. Beijing: China Statistics Press, 2014 (in Chinese)



[2]陈之贵. 啤酒厂加强麦芽质量控制的措施. 啤酒科技, 2002, (7): 24–25



Chen Z G. Malt quality control in brewery. Beer Sci Technol, 2002, (7): 24–25 (in Chinese)



[3]廖加宁. 麦芽对啤酒质量的影响及控制. 啤酒科技, 2006, (5): 31–33



Liao J N. Effect of malt on beer quality and the practice of quality control. Beer Sci Technol, 2006, (5): 31–33 (in Chinese)



[4]冯景章. 麦芽质量统计分析以及企业对大麦麦芽质量的要求与控制. 啤酒科技, 2006, (9): 17–19



Feng J Z. Analysis of malt quality and its requirement and control in beer enterprises. Beer Sci Technol, 2006, (9): 17–19 (in Chinese)



[5]颜启传, 黄亚军, 徐媛. 试用 ISTA 推荐的种子醇溶蛋白电泳方法鉴定大麦和小麦品种. 作物学报, 1992, 18: 61–68



Yan Q C, Huang Y J, Xu Y. Cultivar identification of barley and wheat with standard reference method from International Seed Test Association (ISTA). Acta Agron Sin, 1992, 18: 61–68 (in Chinese with English abstract)



[6]滕晓月, 陶龙兴, 孙雷心. 小麦品种的蛋白电泳鉴定. 作物学报, 1988, 14: 322–327



Teng X Y, Tao L X, Sun L X. Identification of wheat proteins by electrophoresis. Acta Agron Sin, 1988, 14: 322–328 (in Chinese with English abstract)



[7]佘花娣, 陈景堂, 黄亚群, 祝丽英, 池书敏. 利用DNA指纹图谱进行农作物品种鉴定的研究进展. 河北农业大学学报, 2003, 26(增刊): 28–30



She H D, Chen J T, Huang Y Q, Zhu L Y, Chi S M. Progress on variety identification of crop using DNA fingerprinting. J Agric Univ Hebei, 2003, 26(suppl): 28–30 (in Chinese with English abstract)



[8]谷方红, 张五九, 冯景章, 林智平.酿造大麦品种鉴定技术的研究. 啤酒科技, 2003, (11): 27–29



Gu F H, Zhang W J, Feng J Z, Lin Z P. Identification technologies of malt barley varieties. Beer Sci Technol, 2003, (11): 27–29 (in Chinese)



[9]Tinker N A, Fortin M G, Mather D E. Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet, 1993, 85: 976–984



[10]黄祥斌, 杨华林.采用PCR-RAPD技术鉴定16种北美啤酒大麦. 啤酒科技, 2003, (10): 52–54



Huang X B, Yang H L. Identification of 16 beer barley varieties using PCR-RAPD technique. Beer Sci Technol, 2003, (10): 52–54 (in Chinese)



[11]Russell J, Fuller J, Young G, Thomas B, Macaulay M, Waugh R, Powell W, Taramino G. Discriminating between barley genotypes using microsatellite markers. Genome, 1997, 40: 442–450



[12]兰青阔, 张桂华, 王永, 赵新, 朱珠, 崔兴华, 郭永泽, 程奕. 基于SNP标记的黄瓜杂交种纯度鉴定方法. 中国蔬菜, 2012, (6): 58–63



Lan Q K, Zhang G H, Wang Y, Zhao X, Zhu Z, Cui X H, Guo Y Z, Cheng Y. SNP-based molecular assay for cucumber hybrid seed purity identification by pyrosequencing. China Vegetables, 2012, (6): 58–63 (in Chinese with English abstract)



[13]Yu H H, Xie W B, Li J, Zhou F S, Zhang Q F. A whole-genome SNP array (RICE6K) for genomic breeding in rice. Plant Biotechnol J, 2014, 12: 28-37



[14]谷方红, 李海峰, 张五九, 林智平, 冯景章.采用微卫星技术进行大麦及麦芽的品种鉴定. 啤酒科技, 2005, (11): 26–28



Gu F H, Li H F, Zhang W J, Lin Z P, Feng J Z. Identification of barley and malt varieties using SSR markers. Beer Sci Technol, 2005, (11): 26–28 (in Chinese)



[15]Giancola S, McKhann H I, Bérard A, Camilleri C, Durand S, Libeau P, Roux F, Reboud X, Gut I G, Brunel D. Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploid plants. Theor Appl Genet, 2006, 112: 1115–1124



[16]袁芳, 徐进, 季林丹, 费丽娟, 刘盼盼, 张莉娜. Tm-shift基因分型方法在遗传学中的应用. 遗传, 2012, 34: 1484–1490



Yuan F, Xu J, Ji L D, Fei L J, Liu P P, Zhang L N. Application of Tm-shift genotyping method in genetic studies. Hereditas (Beijing), 2012, 34: 1484–1490 (in Chinese with English abstract)



[17]Guo G G, Dondup D W, Zhang L S, Hu S, Yuan X M, Zhang J. Identification of SNPs in barley (Hordeum vulgare L.) by deep sequencing of six reduced representation libraries. Crop J, 2014, 2: 419–425



[18]Lister D L, Jones H, Jones M K, O'Sullivan D M, Cockram J. Analysis of DNA polymorphism in ancient barley herbarium material: validation of the KASP SNP genotyping platform. Taxon, 2013, 62: 779–789



[19]马蕾蕾, 徐世昌, 曹远银. 植物种子DNA快速提取新方法. 沈阳农业大学学报, 2005, 36: 626–627



Ma L L, Xu S C, Cao Y Y. A new protocol for fast DNA extraction from plant seeds. J Shenyang Agric Univ, 2005, 36: 626–627 (in Chinese with English abstract)



[20]Sharp P J, Kreis M, Shewry P R, Gale M D. Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet, 1988, 75: 286–290

[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[3] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[4] MA Hong-Bo, LIU Dong-Tao, FENG Guo-Hua, WANG Jing, ZHU Xue-Cheng, ZHANG Hui-Yun, LIU Jing, LIU Li-Wei, YI Yuan. Application of Fhb1 gene in wheat breeding programs for the Yellow-Huai Rivers valley winter wheat zone of China [J]. Acta Agronomica Sinica, 2022, 48(3): 747-758.
[5] WANG Yin, FENG Zhi-Wei, GE Chuan, ZHAO Jia-Jia, QIAO Ling, WU Bang-Bang, YAN Su-Xian, ZHENG Jun, ZHENG Xing-Wei. Identification of seedling resistance to stripe rust in wheat-Thinopyrum intermedium translocation line and its potential application in breeding [J]. Acta Agronomica Sinica, 2021, 47(8): 1511-1521.
[6] JIANG Peng, ZHANG Xu, WU Lei, HE Yi, ZHANG Ping-Ping, MA Hong-Xiang, KONG Ling-Rang. Genetic analysis for yield related traits of wheat (Triticum aestivum L.) based on a recombinant inbred line population from Ningmai 9 and Yangmai 158 [J]. Acta Agronomica Sinica, 2021, 47(5): 869-881.
[7] HE Jun-Yu, YIN Shun-Qiong, CHEN Yun-Qiong, XIONG Jing-Lei, WANG Wei-Bin, ZHOU Hong-Bin, CHEN Mei, WANG Meng-Yue, CHEN Sheng-Wei. Identification of wheat dwarf mutants and analysis on association between the mutant traits of the dwarf plants [J]. Acta Agronomica Sinica, 2021, 47(5): 974-982.
[8] WANG Heng-Bo, CHEN Shu-Qi, GUO Jin-Long, QUE You-Xiong. Molecular detection of G1 marker for orange rust resistance and analysis of candidate resistance WAK gene in sugarcane [J]. Acta Agronomica Sinica, 2021, 47(4): 577-586.
[9] WANG Rui, SHI Long-Jian, TIAN Hong-Li, YI Hong-Mei, YANG Yang, GE Jian-Rong, FAN Ya-Ming, REN Jie, WANG Lu, LU Da-Lei, ZHAO Jiu-Ran, WANG Feng-Ge. Identification of SNP core primer and establishment of high throughput detection scheme for purity identification in maize hybrids [J]. Acta Agronomica Sinica, 2021, 47(4): 770-779.
[10] ZHANG Xue-Cui, SUN Su-Li, LU Wei-Guo, LI Hai-Chao, JIA Yan-Yan, DUAN Can-Xing, ZHU Zhen-Dong. Identification of resistance gene against phytophthora root rot in new soybean lines breeded in Henan province [J]. Acta Agronomica Sinica, 2021, 47(2): 275-284.
[11] GUO Qing-Qing, ZHOU Rong, CHEN Xue, CHEN Lei, LI Jia-Na, WANG Rui. Location and InDel markers for candidate interval of the orange petal gene in Brassica napus L. by next generation sequencing [J]. Acta Agronomica Sinica, 2021, 47(11): 2163-2172.
[12] HUANG Yi-Wen, DAI Xu-Ran, LIU Hong-Wei, YANG Li, MAI Chun-Yan, YU Li-Qiang, YU Guang-Jun, ZHANG Hong-Jun, LI Hong-Jie, ZHOU Yang. Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat [J]. Acta Agronomica Sinica, 2021, 47(11): 2080-2090.
[13] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[14] JIANG Peng,HE Yi,ZHANG Xu,WU Lei,ZHANG Ping-Ping,MA Hong-Xiang. Genetic analysis of plant height and its components for wheat (Triticum aestivum L.) cultivars Ningmai 9 and Yangmai 158 [J]. Acta Agronomica Sinica, 2020, 46(6): 858-868.
[15] HU Mao-Long, CHENG Li, GUO Yue, LONG Wei-Hua, GAO Jian-Qin, PU Hui-Ming, ZHANG Jie-Fu, CHEN Song. Development and application of the marker for imidazolinone-resistant gene in Brassica napus [J]. Acta Agronomica Sinica, 2020, 46(10): 1639-1646.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!