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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1237-1245.doi: 10.3724/SP.J.1006.2015.01237


Effects of Double Cropping of Wheat and Cotton on Fat and Protein Metabolism of Cotton Embryo

SHEN Tian-Yao,DU Xiang-Bei,YANG Hong-Kun,ZHANG Yu-Xiao,ZHENG Yu-Fei,ZHOU Zhi-Guo,CHEN Bing-Lin*   

  1. Nanjing Agricultural University / Key Laboratory of Crop Physiology Ecology and Production Management, Ministry of Agriculture, Nanjing 210095, China
    Abstract: To provide a theoretical basis for improving the quality of cottonseed under the condition of stabilizing the cotton yield
  • Received:2015-01-08 Revised:2015-05-04 Online:2015-08-12 Published:2015-06-03
  • Contact: 陈兵林, E-mail: blchen@njau.edu.cn, Tel: 025-84396129 E-mail:stychopin@163.com


To provide a theoretical basis for improving the quality of cottonseed under the condition of stabilizing the cotton yield and quality in wheat-cotton double cropping region of China, we studied the effects of the cropping system on fat and protein metabolism of cotton embryo. Field experiments were conducted in 2012 and 2013 at the cotton experimental station of Dafeng City, Jiangsu Province, China, using cotton cultivars Siza 3 (Mid-late maturity) and CCRI 50 (early maturity) with four cropping systems monocropping cotton (CK) as control, and three wheat-cotton double cropping systems were intercropped cotton (IC), transplanted cotton (TC) and direct-seeded cotton (DC). The accumulation of cotton embryo fat and protein, and key enzyme activities as well as their correlation were measured. The results showed that cotton embryo fat content of the double-cropped cotton was lower than that of CK, with the order of IC>TC>DC. Besides, the cotton embryo protein content of IC, TC was higher than that of CK, however, that of DC was lower than that of CK. Moreover, the contents of cotton embryo fat and protein were higher in CCRI 50 than in Siza 3. The double-cropped cotton had lower phosphatidate phosphatase (PPase) and glucose-6-phosphate dehydrogenase (G6PDH) but higher phosphoenolpyruvate carboxylase (PEPC) activities, compared with CK. Furthermore, the activities of glutamine synthetase (GS) and glutamate synthase (GOGAT) of IC and TC were higher than those of CK, however, those of DC were lower. Moreover, the activities of PPase, G6PDH, GS and GOGAT were higher in CCRI 50 than in Siza 3, but the PEPC activity had opposite trend between them. The content of cotton embryo fat was significantly and positively correlated with PPase and G6PDH activities, and significantly and negatively correlated with PEPC activity. In addition, the content of cotton embryo protein was significantly and positively correlated with G6PDH, GS and GOGAT activities. In summary, wheat-cotton intercropping system with mid-maturity cotton cultivars should be utilized to increase the quality of cottonseed on the basis of the stable cotton yield and quality in wheat–cotton region double cropping of China.

Key words: Wheat-cotton cropping system, Cotton embryo, Fat, Protein, Key enzyme

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