Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (02): 303-310.doi: 10.3724/SP.J.1006.2016.00303

• RESEARCH NOTES • Previous Articles    

Analysis of QTL for Fatty Acid Contents under Different Environments in Soybean

LEI Ya-Kun1,2,**,LIU Bing-Qiang1,**,DI Rui1,YAN Long1,YANG Chun-Yan1,HAO Dong-Xu3,ZHANG Meng-Chen1,*   

  1. 1 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences / Shijiazhuang Branch Center of National Center for Soybean Improvement / Hebei Genetic Breeding Laboratory, Shijiazhuang 050031, China; 2 Institute of Agricultural Information and Economy, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 3 Hebei Econmy Management School, Shijiazhuang 050071, China
  • Received:2015-07-09 Revised:2015-11-20 Online:2016-02-12 Published:2015-12-08
  • Contact: 张孟臣, E-mail: zhangmengchen@hotmail.com, Tel: 0311-87670653 E-mail:yakun0628@163.com
  • Supported by:

    This study was support by the National High Technology Research and Development Program of China (863 Program) (2006AA10Z1B3, 2012AA101106) and the National Natural Science Foundation of China (31000719), the Major Project of China on New Varieties of GMO Cultivation (2014ZX0800402B) and the Special Program of Modern Agro-industry Technology System (CARS-004-PS06), the Doctoral Scientific Research Foundation of Hebei Province (F13E006) and the Program for Excellent Young Talents in Hebei Province.

RichHTML

PDF

910

Abstract:

Soybean is an important crop which contributes about 60% of the world’s oilseed production. The quality of soybean oil depends on the relative composition of fatty acid in seeds. A population of recombinant inbred lines  derived from a cross of Jidou 12 × Heidou was grown in two environments and the seed samples from the environments were evaluated for fatty acid contents. Simple sequence repeat (SSR) markers were used to construct genetic linkage map. A total of 16 QTLs underlying fatty acid contents were identified by CIM and MCIM methods using Windows QTL cartographer 2.5 and QTL Network-2.0 software at Sanya and Shijiazhuang locations, respectively. These QTLs were scattered on linkage groups A2, B2, C2, F, G, I, L. According to the two environment combined data, 13 QTLs were detected using two mapping methods. Nine of them were common in the results from two methods. Another QTL associated with stearic acid content, named as Ste-1, located on LG B2 and flanked by Satt168 and Satt556, was stable across two locations, also. QTL Ste-1 could explain 12% of the phenotypic variation at both locations. This study is helpful to improve fatty acid composition in soybean.

Key words: Soybean, Fatty acids, Quantitative trait loci, Environment

[1] 宋晓昆, 张颖君, 闫龙, 杨春燕, 郑艳艳, 蒋春志, 荆慧贤, 张孟臣, 黄占景. 大豆脂肪酸组份相关、变异特点分析. 华北农学报, 2010, 25 (增刊): 68–73



Song X K, Zhang Y J, Yan L, Yang C Y, Zheng Y Y, Jiang C Z, Jing H X, Zhang M C, Huang Z J. A study on correlation and variability of fatty acid composition contents of soybean cultivars. Acta Agric Boreali-Sin, 2010, 25(suppl): 68–73 (in Chinese with English abstract)



[2] 吕景良, 邵荣春, 吴百灵, 梁岐, 吴桂荣. 东北地区大豆品种资源脂肪酸组成的分析研究. 作物学报, 1990, 16: 349–355



Lü J L, Shao R C, Wu B L, Liang Q, Wu G R. Fatty acid compo-sition analysis of soybean germplasm resources in Northeast. Acta Agron Sin, 1990, 16: 349–355 (in Chinese with English abstract)



[3] Brummer E C , Graef G L, Orf J, Wilcox J R, Shoemaker R C. Mapping QTL for seed protein and oil content in eight soybean populations. Crop Sci, 1997, 37: 370–378



[4] Diers B W, Shoemaker R C. Restriction fragment length polymorphism analysis of soybean fatty acid content. J Am Oil Chem Soc, 1992, 69: 1242–1244



[5] Li Z L, Wilson R F, Rayford W E, Boerma H R. Molecular mapping genes conditioning reduced palmitic acid content in N87-2122-4 soybean. Crop Sci, 2002, 42: 373–378



[6] Spencer M M, Pantalone V R, Meyer E J. Mapping the Fas locus controlling stearic acid content in soybean. Theor Appl Genet, 2003, 106: 615–619



[7] 郑永战, 盖钧镒, 卢为国, 李卫东, 周瑞宝, 田少君. 大豆脂肪及脂肪酸组分含量的QTL定位. 作物学报, 2006, 32: 1823–1830



Zheng Y Z, Gai J Y, Lu W G, Li W D, Zhou R B, Tian S J. QTL mapping for fat and fatty acid composition contents in soybean. Acta Agron Sin, 2006, 32: 1823–1830 (in Chinese with English abstract)



[8] Orf J H, Diers B W, Boerma H R. Genetic improvement：conventionaland molecular based strategies. In: Boerma H R, Specht J E, eds. Soybeans: Improvement, Production, and Uses, 3rd Edn. 出版地: American Society of Agronomy Inc., 2004. pp 417–450



[9] 宋万坤, 王晶, 朱命喜, 齐照明, 刘春燕, 陈庆山, 胡国华. 大豆脂肪酸组分相关QTL元分析. 大豆科学, 2009, (5): 774–778



Song W K, Wang J, Zhu M X, Qi Z M, Liu C Y, Chen Q S, Hu G H. Meta-analysis of fatty acid QTLs in soybean. Soybean Sci, 2009, (5): 774–778 (in Chinese with English abstract)



[10] 于福宽. 大豆种质脂肪酸主要组分鉴定与QTL标记定位. 中国农业科学院硕士学位论文, 北京, 2011



Yu F K. Mapping QTLs and Screening Germplasm for the Major Fatty Acid Components in Soybean Seeds. MS Thesis of Graduate School of Chinese Academy of Agricultural Sciences, Beijng, China, 2011 (in Chinese with English abstract)



[11] 朱军. 运用混合线性模型定位复杂数量性状基因的方法. 浙江大学学报(自然科学版), 1999, 33: 327–335



Zhu J. Methods for locating complex quantitative trait genes using hybrid linear model. J Zhejiang Univ (Nat Sci), 1999, 33: 327–335 (in Chinese with English abstract)



[12] 单大鹏, 齐照明, 邱红梅, 单彩云, 刘春燕, 胡国华, 陈庆山. 大豆油分含量相关的QTL间的上位效应和QE互作效应. 作物学报, 2008, 34: 952–957



Shan D P, Qi Z M, Qiu H M, Shan C Y, Liu C Y, Hu G H, Chen Q S. Epistatic effects of QTLs and QE interaction effects on oil content in soybean. Acta Agron Sin, 2008, 34: 952–957 (in Chinese with English abstract)



[13] 苗兴芬. 多年多点条件下大豆脂肪酸含量QTL分析. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2010



Miao X F. QTL Analysis of Fatty Acids Content of Soybean in Multiple Years and Sites. MS Thesis of Northeast Agricultural University, Heilongjiang, China, 2010 (in Chinese with English abstract)



[14] 张颖君, 高慧敏, 蒋春志, 胡梦芸, 刘兵强, 李辉. 大豆种子脂肪酸含量的快速测定. 大豆科学, 2008, 27: 859–862



Zhang Y J, Gao H M, Jiang C Z, Hu M Y, Liu B Q, Li H. Fast analysis on fatty acids of soybean seed by gas chromatography. Soybean Sci, 2008, 27: 859–862 (in Chinese with English abstract)



[15] Cregan P B, Jarvik T, Bush A L, Shoemaker R C, Lark K G, Kahler A L, Kaya N, van Toai T T, Lohnes D G, Chung J. An integrated genetic linkage map of the soybean genome. Crop Sci, 1999, 39: 1464–1490



[16] 雷雅坤, 闫龙, 杨春燕, 宋晓昆, 张孟臣, 黄占景. 大豆公共遗传图谱C1连锁群SSR标记空白区段的填补. 华北农学报, 2012, 27(6): 5–10



Lei Y K, Yan L, Yang C Y, Song X K, Zhang M C, Huang Z J. Complete the blank section with SSR markers on linkage group C1 of public genetic map in soybean. Acta Agric Boreali-Sin, 2012, 27(6): 5–10 (in Chinese with English abstract)



[17] Maria J M, Joseph W B, Roger H B. Molecular mapping and conrmation of QTLs associated with oleic acid content in N00-3350 soybean. Crop Sci, 2008, 48: 2223–2234



[18] 宋晓昆. 大豆脂肪酸组分遗传及QTL分析. 河北师范大学硕士学位论文, 河北石家庄, 2010



Song X K. Genetic Analysis and QTL Mapping of Fatty Acid Composition Contents in Soybean. MS Thesis of Hebei Normal University, Shijiazhuang, China, 2010 (in Chinese with English abstract)



[19] 邹筱. 大豆脂肪和脂肪酸主要组分含量QTL定位. 中国农业科学院研究生院, 北京, 2014



Zou Y. Quantitative Trait Loci of Oil and Main Fatty Acid Components in Soybean. MS Thesis of Gratude School of Chinese Academy of Agricultural Sciences, Beijing, 2014 (in Chinese with English abstract)



[20] Fulton T M, Beck Bunn T, Emmatty D, Eshed Y, Lopez J, Petiard V, Uhlig J, Zamir D, Tanksley S D. QTL analysis of an advanced backcross of Lycopersicon peruvianumto the cultivated tomato and comparisons with QTLs found in otherwild species. Theor Appl Genet, 1997, 95: 881–894



[21] Jansen R C, Van Ooijen J W, Stam P, Lister C, Dean C. Genotype-by-environment interaction in genetic mapping of multi-ple quantitative trait loci. Theor Appl Genet, 1995, 91: 33–37



[22] 陈霞. 黑龙江省主栽大豆品种脂肪、脂肪酸组份的测定及其相关性的分析. 大豆科学, 1996, 15: 92–95



Chen X. Determination of fat and fatty acid composition of soybean cultivars in eilongjiang province and analysis of their correlation. Soybean Sci, 1996, 15: 92–95 (in Chinese with English abstract)



[23] 李文滨, 郑宇宏, 韩英鹏. 大豆种质资源脂肪酸组分含量及品质性状的相关性分析. 大豆科学, 2008, 27(5): 740–745



Li W B, Zheng Y H, Han Y P. Analysis of fatty acid composition and other quality traits in soybean varieties developed in heilongjiang province. Soybean Sci, 2008, 27: 740–745 (in Chinese with English abstract)



[24]  徐豹, 庄炳昌, 路琴华, 胡传璞. 中国大豆主要生产品种蛋白质、脂肪及其组份的相关分析. 大豆科学, 1988, 7: 175–183



Xu B, Zhuang B C, Lu Q H, Hu C P. Correlation analysis between protein content and its composition, fat content and its composition in current soybean cultivar seeds in China. Soybean Sci, 1988, 7: 175–183 (in Chinese with English abstract)



[25]  张忠臣, 战秀玲, 陈庆山, 滕卫丽, 杨庆凯, 李文滨, 刘迎雪, 郭强, 张丽娜. 大豆QTL定位的研究进展. 大豆科学, 2004, 23: 222–227



Zhang Z Y, Zhan X L, Chen Q S, Teng W L, Yang Q K, Li W B, Liu Y X, Guo Q, Zhang L N. A review of QTL research in soybean. Soybean Sci, 2004, 23: 222–227



[26] 单大鹏, 朱荣胜, 陈立君, 齐照明, 刘春燕, 胡国华, 陈庆山. 大豆蛋白质含量相关QTL间的上位效应和QE互作效应. 作物学报, 2009, 35: 41–47



Shan D Y, Zhu R S, Chen L J, Qi Z M, Liu C Y, Hu G H, Chen Q S. Epistatic effects and QE interaction effects of QTLs for protein content in soybean. Acta Agron Sin, 2009, 35: 41–47 (in Chinese with English abstract)
[1] CHEN Ling-Ling, LI Zhan, LIU Ting-Xuan, GU Yong-Zhe, SONG Jian, WANG Jun, QIU Li-Juan. Genome wide association analysis of petiole angle based on 783 soybean resources (Glycine max L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1333-1345.
[2] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[3] YU Chun-Miao, ZHANG Yong, WANG Hao-Rang, YANG Xing-Yong, DONG Quan-Zhong, XUE Hong, ZHANG Ming-Ming, LI Wei-Wei, WANG Lei, HU Kai-Feng, GU Yong-Zhe, QIU Li-Juan. Construction of a high density genetic map between cultivated and semi-wild soybeans and identification of QTLs for plant height [J]. Acta Agronomica Sinica, 2022, 48(5): 1091-1102.
[4] LI A-Li, FENG Ya-Nan, LI Ping, ZHANG Dong-Sheng, ZONG Yu-Zheng, LIN Wen, HAO Xing-Yu. Transcriptome analysis of leaves responses to elevated CO2 concentration, drought and interaction conditions in soybean [Glycine max (Linn.) Merr.] [J]. Acta Agronomica Sinica, 2022, 48(5): 1103-1118.
[5] PENG Xi-Hong, CHEN Ping, DU Qing, YANG Xue-Li, REN Jun-Bo, ZHENG Ben-Chuan, LUO Kai, XIE Chen, LEI Lu, YONG Tai-Wen, YANG Wen-Yu. Effects of reduced nitrogen application on soil aeration and root nodule growth of relay strip intercropping soybean [J]. Acta Agronomica Sinica, 2022, 48(5): 1199-1209.
[6] WANG Hao-Rang, ZHANG Yong, YU Chun-Miao, DONG Quan-Zhong, LI Wei-Wei, HU Kai-Feng, ZHANG Ming-Ming, XUE Hong, YANG Meng-Ping, SONG Ji-Ling, WANG Lei, YANG Xing-Yong, QIU Li-Juan. Fine mapping of yellow-green leaf gene (ygl2) in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2022, 48(4): 791-800.
[7] LI Rui-Dong, YIN Yang-Yang, SONG Wen-Wen, WU Ting-Ting, SUN Shi, HAN Tian-Fu, XU Cai-Long, WU Cun-Xiang, HU Shui-Xiu. Effects of close planting densities on assimilate accumulation and yield of soybean with different plant branching types [J]. Acta Agronomica Sinica, 2022, 48(4): 942-951.
[8] DU Hao, CHENG Yu-Han, LI Tai, HOU Zhi-Hong, LI Yong-Li, NAN Hai-Yang, DONG Li-Dong, LIU Bao-Hui, CHENG Qun. Improving seed number per pod of soybean by molecular breeding based on Ln locus [J]. Acta Agronomica Sinica, 2022, 48(3): 565-571.
[9] ZHOU Yue, ZHAO Zhi-Hua, ZHANG Hong-Ning, KONG You-Bin. Cloning and functional analysis of the promoter of purple acid phosphatase gene GmPAP14 in soybean [J]. Acta Agronomica Sinica, 2022, 48(3): 590-596.
[10] WANG Juan, ZHANG Yan-Wei, JIAO Zhu-Jin, LIU Pan-Pan, CHANG Wei. Identification of QTLs and candidate genes for 100-seed weight trait using PyBSASeq algorithm in soybean [J]. Acta Agronomica Sinica, 2022, 48(3): 635-643.
[11] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[12] ZHANG Guo-Wei, LI Kai, LI Si-Jia, WANG Xiao-Jing, YANG Chang-Qin, LIU Rui-Xian. Effects of sink-limiting treatments on leaf carbon metabolism in soybean [J]. Acta Agronomica Sinica, 2022, 48(2): 529-537.
[13] YU Tao-Bing, SHI Qi-Han, NIAN-Hai , LIAN Teng-Xiang. Effects of waterlogging on rhizosphere microorganisms communities of different soybean varieties [J]. Acta Agronomica Sinica, 2021, 47(9): 1690-1702.
[14] SONG Li-Jun, NIE Xiao-Yu, HE Lei-Lei, KUAI Jie, YANG Hua, GUO An-Guo, HUANG Jun-Sheng, FU Ting-Dong, WANG Bo, ZHOU Guang-Sheng. Screening and comprehensive evaluation of shade tolerance of forage soybean varieties [J]. Acta Agronomica Sinica, 2021, 47(9): 1741-1752.
[15] CAO Liang, DU Xin, YU Gao-Bo, JIN Xi-Jun, ZHANG Ming-Cong, REN Chun-Yuan, WANG Meng-Xue, ZHANG Yu-Xian. Regulation of carbon and nitrogen metabolism in leaf of soybean cultivar Suinong 26 at seed-filling stage under drought stress by exogenous melatonin [J]. Acta Agronomica Sinica, 2021, 47(9): 1779-1790.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd