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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (12): 1754-1763.doi: 10.3724/SP.J.1006.2016.01754

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Characterization and Gene Mapping of a Thermo-sensitive AlbinoLeaf Mutant tsa1 in Rice

LIU Yu-Long1,**,LIU Feng1,**,ZHOU Kun-Neng3,SU Xiao-Mei1,FANG Xian-Wen2,ZHANG Yun-Hui2,*,BAO Yi-Qun1,*   

  1. 1 College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu Agricultural Germplasm Resources Conservation and Utilization Platform, Nanjing 210014, China; 3 Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2016-03-21 Revised:2016-07-11 Online:2016-12-12 Published:2016-08-24
  • Contact: Bao Yiqun,E-mail:baoyiqun@njau.edu.cn,Tel:025-84396795;Zhang Yunhui,E-mail:zyhrice@163.com,Tel:025-84390321 E-mail:2013116120@njau.edu.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation (31401036), the Natural Science Foundation of Jiangsu Province (BK20130706), the Jiangsu Province Agricultural Science and Technology Independent Innovation Foundation [CX(14)5005], the Jiangsu Academy of Agricultural Sciences Fundamental Research Projects [ZX(15)4015], and the Natural Science Foundation of Anhui Province (1408085MKL62).

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Abstract:

A thermo-sensitive albino leaf mutant tsa1 was obtained from Ningjing36 induced by Ethyl methane sulfonate (EMS). The mutant developed albinotic leaves under low temperature(20-24°C), and the color of mutant leaves was comparable with the wild-type under high temperature (28-32°C). Compared with wild type, the chlorophyll a and carotene contents of mutant albinotic leaves obviously decreased under low temperature condition. With microscopeobservation, the quantity of normal chloroplast significantly decreased, besides, there were a large number of small abnormal chloroplasts in albinotic leaves. The abnormal chloroplast in albinotic tissue of mutant had no obvious thylakoid stack under transmission electron microscopy. These results indicate that tsa1 mutant has defect on chloroplast development under low temperature. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. Genetic mapping of the mutant gene was conducted using 368 recessive individuals from F2 population of tsa1/Nanjing 11, the target gene was finally located in a 163 kb region on the chromosome 5. This study provides a basis for map-based cloning and functional analysis of the target gene.

Key words: Rice(OryzasativaL.), Thermo-sensitive, Albino, Mutant, Chloroplastdevelopment

[1]ReyesprietoA,WeberAP,BhattacharyaD.Theoriginandestablishmentoftheplastidinalgaeandplants.AnnuRevGenet,2007,41:147–168
[2]WataruS,Shin-yaM,PaulJ.Chloroplastbiogenesis:Controlofplastiddevelopment,proteinimport,divisionandinheritance.AmSocPlantBiologists,2008,6(6):e0110
[3]BealeSI.Greengenesgleaned.TrendsPlantSci,2005,10:309–312
[4]KhushGS.Strategiesforincreasingtheyieldpotentialofcereals:caseofriceasanexample.PlantBreed,2013,132:433–436
[5]LeisterD.Chloroplastresearchinthegenomicage.TrendsGenet,2003,19:47–56
[6]KurataN,MiyoshiK,NonomuraKI,YamazakiY,ItoY.Ricemutantsandgenesrelatedtoorgandevelopment,morphogenesisandphysiologicaltraits.PlantCellPhysiol,2005,46:48–62
[7]KusumiK,MizutaniA,NishimuraM,KohI.AVirescentgeneV1determinestheexpressiontimingofplastidgenesfortranscription/translationapparatusduringearlyleafdevelopmentinrice.PlantJ,1997,12:1241–1250
[8]SugimotoH,KusumiK,TozawaY,YazakiJ,KishimotoN,KikuchiS,IbaK.Thevirescent-2mutantinhibitsfortheplastidtrancriptsfortheplastidgeneticsystematanearlystageofchloroplastdifferentiation.PlantCellPhysiol,2004,45:985–996
[9]王军,杨杰,陈志德,范方军,朱金燕,杨金欢,仲维功.水稻白化转绿突变体v13(t)的生理特性和基因定位.中国农业科学,2014,44:1973–1979
WangJ,YangJ,ChenZD,FanFJ,ZhuJY,YangJH,ZhongWG.Physiologicalcharacterandgenefinemappingofavirescentmutantv13(t)inrice(OryzasativaL.).SciAgricSin,2014,44:1973–1979(inChinesewithEnglishabstract)
[10]崔海瑞,夏英武,高明尉.温度对水稻突变体W1叶色及叶绿素生物合成的影响.核农学报,2001,15:269–273
CuiHR,XiaYW,GaoMW.EffectoftemperatureonleafcolorandchlorophyllbiosynthesisofricemutantW1.ActaAgricNuclSin,2001,15:269–273(inChinesewithEnglishabstract)
[11]舒庆尧,刘贵付,夏英武.温敏水稻叶色突变体的研究.核农学报,1996,10:6–10
ShuXQ,LiuGF,XiaYW.Researchadvancesinthermo-sensitiveleafcolorationmutantsinrice.ActaAgricNuclSin,1996,10:6–10(inChinese)
[12]董彦君,董文其,张小明,石守鋆,张宏德.突变体Fan5苗色低温敏感性状的遗传分析.中国水稻科学,1995,9:249–250
DongYJ,DongWQ,ZhangXM,ShiSM,ZhangHD.Geneticanalysisoflow-temperature-sensitiveseedling-colourcharacterinthemutantFan5.ChinJRiceSci,1995,9:249–250(inChinesewithEnglishabstract)
[13]GongXD,SuQQ,LinDZ,JiangQ,XuJL,ZhangJH,TengS,DongYJ.ThericeOsV4encodinganovelpentatricopeptiderepeatproteinisrequiredforchloroplastdevelopmentduringtheearlystageundercoldstress.JIntegrPlantBiol,2014,56:400–410
[14]JiangQ,MeiJ,GongXD,XuJL,ZhangJH,TengS,LinDZ,DongYJ.ImportanceofthericeTCD9encodingasubunitofchaperoninprotein60(Cpn60α)forearlyleafstage.PlantSci,2013(215–216):172–179
[15]SongJ,WeiXJ,ShaoGN,ShengZ,ChenD,LiuC,JiaoG,XieL,TangS,HuP.ThericenucleargeneWLP1encodingachloroplastribosomeL13proteinisneededforchloroplastdevelopmentinricegrownunderlowtemperaturecondition.PlantMolBiol,2014,84:301–314
[16]李育红,王宝和,戴正元,李爱宏,刘广青,左示敏,张洪熙,潘学彪.一个水稻新型叶色突变体的形态结构和遗传定位.中国水稻科学,2011,25:587–593
LiYX,WangBH,DaiZY,LiAH,LiuGQ,ZuoSM,ZhangHX,PanXB.Morphologicalstructureandgeneticmappingofanewleafcolormutantinrice.ChinJRiceSci,2011,25:587–593(inChinesewithEnglishabstract)
[17]李超,林冬枝,董彦君,叶胜海,张小明.一个水稻苗期温敏白色条斑叶突变体的遗传分析及基因定位.中国水稻科学,2010,24:223–227
LiC,LinDZ,DongYJ,YeSH,ZhangXM.Geneticanalysisandmappingofathermo-sensitivewhitestripeleafmutantattheseedlingstageinrice(Oryzasativa).ChinJRiceSci,2010,24:223–227(inChinesewithEnglishabstract)
[18]王平荣,王兵,孙小秋,孙昌辉,万春美,马晓智,邓晓建.水稻白化转绿基因gra75的精细定位和生理特性分析.中国农业科学,2013,46:225–232
WangRP,WangB,SunXQ,SunCH,WanCM,MaXZ,DengXJ.Finemappingandphysiologicalcharacteristicsofagreen-revertiblealbinogenegra75inrice.SciAgricSin,2013,46:225–232(inChinesewithEnglishabstract)
[19]LichtenthalerHK.Chlorophyllsandcarotenoids:pigmentsofphotosyntheticbiomembranes.MethodsEnzymol,1987,148:350–382
[20]ChoryJ.Lightsignalsinleafandchloroplastdevelopment:photoreceptorsanddownstreamresponsesinsearchofatransductionpathway.NewBiol,1991,3:538–548
[21]JohneA.Insuranceproductdevelopment:Managingthechanges.IntJBankMarketing,1993,11(3):5–14
[22]KusumiK,ChonoY,ShimadaH,GotohE,TsuyamaM,IbaK.Chloroplastbiogenesisduringtheearlystageofleafdevelopmentinrice.PlantBiotechnol,2010,27:85–90
[23]WuZ,ZhangX,HeB,DiaoLP,ShengSL,WangJL,GuoXP,SuN,WangLF,JiangL,WangCM,ZhaiHQ,WanJM.Achlorophyll-deficientricemutantwithimpairedchlorophyllideesterificationinchlorophyllbiosynthesis.PlantPhysiol,2007,145:29–40
[24]YooJH,ParkJH,ChoSH,YooSC,LiJJ,ZhangHT,KimKS,KohHJ,PaekNC.Thericebrightgreenleaf(bgl)locusencodesOsRopGEF10,whichactivatesthedevelopmentofsmallcuticularpapillaeonleafsurfaces.PlantMolBiol,2011,77:631–641
[25]MiyoshiK,ItoY,SerizawaA,KurataN.OsHAP3genesregulatechloroplastbiogenesisinrice.PlantJ,2003,36:532–540
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