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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (10): 1539-1547.doi: 10.3724/SP.J.1006.2018.01539

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns

Fang WANG1,Jing-Sheng CHEN2,Da-Wei LIU3   

  1. 1 College of Life Sciences and Agroforestry, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihar 161006, Heilongjiang, China
    2 Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, China
    3 Agronomy College, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2018-02-05 Accepted:2018-07-20 Online:2018-10-10 Published:2018-07-31
  • Supported by:
    This study was supported by the Heilongjiang Provincial Higher-education Basic Scientific Research Project(2012K-M21)

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Abstract:

To study bacterial community structure of soybean rhizosphere soil in rotation and continuous cropping, 16S rDNA gene were sequenced of soil samples infected soybean cyst nematode from Heilongjiang Province two regions by the second generation of high-throughput sequencing Illumina MiSeq platform. A total of 25 419 OTUs were obtained from six soil samples and classified into 47 phylum, 147 class, and 709 genera. Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes were dominant bacteria, accounting for more than 90% of all soil bacterial communities. The total OTUs and richness were the highest in four years continuous cropping and the lowest in twenty years continuous cropping. The difference of bacteria abundance in different rotational cropping years was not obvious (P > 0.05), but abundance and adversity were significant in continuous cropping (P < 0.05). The relative abundance of Actinobacteria in different rotations was lower than that in continuous cropping, and the relative abundance of Gemmatimonadetes and Bacteroidetes was higher than that in the same area. The relative abundance of soil functional bacteria Bradyrhizobium, Streptomyces, Bacillus, Lysobacter, and Pedomicrobium were higher in different continuous cropping years than those in rotations. Predominant bacterial abundance in long term continuous cropping was more similar with that in rotational cropping.

Key words: soybean, rotation, continuous cropping, bacterial diversity, Illumina MiSeq sequencing

Fig. 1

Rarefaction curves for each sample OTUs at cutoff level of 97% RR: susceptible variety-hemp-resistant variety; RS: susceptible variety-marijuana-susceptible variety; RQ: corn-millet-susceptible variety; C4: continuous cropping 4 years; C10: continuous cropping 10 years; C20: continuous cropping 20 years."

Fig. 2

Abundance distribution curve for six samples RR: susceptible variety-hemp-resistant variety; RS: susceptible variety-marijuana-susceptible variety; RQ: corn-millet-susceptible variety; C4: continuous cropping 4 years; C10: continuous cropping 10 years; C20: continuous cropping 20 years."

Table 1

Sequence statistics and diversity indexes of soil bacteria under different rotational cropping system"

样品
Sample		 有效序列数量
Effective
sequence-number		 OTUs数量
OTUs number (97%)		 Chao指数
Chao index		 Shannon指数
Shannon index
感病品种-大麻-抗病品种(大庆) RR (Daqing) 39530 4243 5247.7 a 10.45 a
感病品种-大麻-感病品种(大庆) RS (Daqing) 37614 4140 5122.7 a 10.57 a
玉米-谷子-感病品种(齐齐哈尔) RQ (Qiqihar) 54387 4229 4972.7 a 10.20 b

Table 2

Sequence statistics and diversity indexes of soil bacteria under different continuous cropping system"

样品
Sample		 有效序列数量
Effective
sequence-number		 OTUs数量
OTUs number (97%)		 Chao指数
Chao index		 Shannon指数
Shannon index
连作4年(大庆) C4 (Daqing) 67271 4928 5659.6 a 10.47 a
连作10年(大庆) C10 (Daqing) 69356 4173 4583.8 b 10.23 b
连作20年(齐齐哈尔) C20 (Qiqihar ) 52387 3706 4147.0 b 10.09 c

Fig. 3

Relative abundance of soil bacterial communities on phylum level of six samples"

Fig. 4

Relative abundance of soil bacterial communities on class level of six samples"

Fig. 5

Heatmap tree for samples cluster analysis"

Fig. 6

Principal component analysis of six soil samples community"

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