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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (9): 1408-1415.doi: 10.3724/SP.J.1006.2020.04074

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of mixed-sowing of near-isogenic lines on the clubroot disease controlling efficiency in rapeseed

GUO Qing-Yun1(), KUAI Jie1,*(), WANG Bo1, LIU Fang2, ZHANG Chun-Yu1, LI Gen-Ze3, ZHANG Yun-Yun3, FU Ting-Dong1, ZHOU Guang-Sheng1   

  1. 1 College of Plant Science and Technology, Huazhong Agricultural University / Key Laboratory of Crop Ecophysiology and Farming System for the Middle Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China
    2 National Agricultural Technology Extension Service Center, Beijing 100125, China
    3 Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China
  • Received:2020-03-21 Accepted:2020-06-02 Online:2020-09-12 Published:2020-06-12
  • Contact: Jie KUAI E-mail:1278245883@qq.com;kuaijie@mail.hzau.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2018YFD1000900);Technological Innovation Major Project of the Science and Technology Department of Hubei Province(2017ABA064);Fundamental Research Funds for the Central Universities(2662017JC005)

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Abstract:

The clubroot disease caused by Plasmodiophora brassicae has become a serious threat to rapeseed production in these recent years. In the previous multi-plot and multi-season field experiments, we found that planting of susceptible and resistant cultivars (near-isogenic lines) at different proportions could significantly suppress the incidence of the clubroot disease. In order to explore the preventive and control effect of mix-sowing of susceptible and resistant cultivars on clubroot disease, and identify the factors affecting mixed sowing. Pot experiments with different distance (0, 2, 4, and 6 cm) between the seeds of the resistant and susceptible cultivars were set by artificial inoculation at the 7 days after germination in this study. The disease incidence, disease index and the root biochemical components of rapeseed were measured after forty-two days. The results were as below: (1) the incidence of the resistant cultivar showed no significant change, while the disease incidence and disease index of the susceptible rapeseed cultivars decreased in the mixed-sowing treatment. Further, the disease incidence was significantly influenced by sowing distance of the two cultivars, which was significantly (P < 0.05) lower than those of the other treatments when mixed-sowed at 0 cm. (2) compared to the single planting, the content of soluble sugar and soluble protein in roots of the mixed-sowing treatment were significantly (P < 0.05) decreased for the two cultivars, and the content of acid-insoluble lignin was significantly increased in susceptible cultivar whereas decreased after increasing in resistant cultivar. (3) the results of correlation analysis showed that the content of soluble sugar and soluble protein were positively correlated with the disease incidence and disease index. The correlation coefficients between disease incidence and soluble sugar and soluble protein content were 0.797 and 0.403, respectively, meanwhile, the correlation coefficients between disease index and soluble sugar and soluble protein content were 0.822 and 0.509, respectively. In conclusion, the findings provided a new idea for prevention of clubroot disease in the field production in rapeseed.

Key words: rapeseed, near-isogenic lines, clubroot disease, sowing distance

Table 1

Disease incidence and disease index for Huashuang 5R and Huashuang 5 in different treatments"

菌种
P. brassicae		 处理
Treatment		 华双5R Huashuang 5R 华双5号 Huashuang 5
发病率
Disease incidence
(%)		 病情分级
Disease severity scale		 病情指数
Disease
index		 发病率
Disease
incidence
(%)		 病情分级
Disease severity scale		 病情指数
Disease index
0 1 2 3 0 1 2 3
绩溪2号
小种
Pathotype 2 from Jixi		 CK 5.0 ab 57 0 1 2 4.4 b 100.0 a 0 0 10 50 94.5 a
T1 3.3 b 58 2 0 0 1.1 d 65.0 c 21 24 9 6 33.3 e
T2 3.3b 58 1 1 0 1.7 d 95.0 b 3 10 8 39 79.4 d
T3 3.3 b 58 0 1 1 2.8 c 95.0 b 3 4 7 46 86.7 c
T4 6.7 a 56 0 1 3 6.1 a 96.7 b 2 4 3 51 90.6 b
均值Mean 4.0 3.0 88.0 72.5
恩施4号
小种
Pathotype 4 from Enshi
 CK 1.7 a 59 1 0 0 0.6 a 100.0 a 0 0 17 43 90.6 a
T1 1.7 a 59 1 0 0 0.6 a 62.5 d 17 18 5 20 34.2 e
T2 1.7 a 59 1 0 0 0.6 a 80.0 c 12 22 9 17 50.6 d
T3 1.7 a 59 1 0 0 0.6 a 81.7 c 11 17 8 24 58.3 c
T4 0.0 b 60 0 0 0 0.0 a 91.7 b 5 16 7 32 70.0 b
均值Mean 1.3 0.5 79.0 53.3

Table 2

Disease incidence and disease index of Bing 409R and Bing 409 in different treatments"

菌种
P. brassicae		 处理
Treatment		 丙409R Bing 409R 丙409 Bing 409
发病率
Disease incidence
(%)		 病情分级
Disease severity scale		 病情指数
Disease index		 发病率
Disease
incidence (%)		 病情分级
Disease severity scale		 病情指数
Disease index
0 1 2 3 0 1 2 3
礼州2号
小种
Pathotype 2 from Lizhou		 CK 16.7 a 51 0 4 5 12.8 a 83.3 a 10 8 12 30 67.8 a
T1 15.0 a 51 2 5 2 10.0 c 70.0 cd 18 9 11 17 48.3 d
T2 16.7 a 50 4 3 3 10.6 c 73.3 c 16 9 7 28 58.1 c
T3 15.0 a 51 2 4 3 10.6 c 78.3 b 13 7 14 26 62.8 b
T4 16.7 a 50 3 3 4 11.7 b 80.0 b 12 6 10 32 67.8 a
均值Mean 15.9 10.7 75.4 59.3
歙县4号
小种
Pathotype 4 from Shexian		 CK 8.3 a 55 3 0 2 5.0 a 100.0 a 0 3 10 47 91.1 a
T1 5.0 b 57 2 1 0 2.2 c 43.3 e 34 9 1 16 32.8 d
T2 6.7 ab 56 1 2 1 4.4 b 48.3 cd 31 12 8 9 30.6 d
T3 8.3 a 55 1 4 0 5.0 a 50.0 bc 30 6 11 13 37.2 c
T4 8.3 a 55 1 4 0 5.0 a 51.7 b 29 4 8 19 42.8 b
均值Mean 7.1 4.2 48.3 35.9

Table 3

Root biochemistry components variation of Huashuang 5R and Huashuang 5 in different treatments"

菌种
P. brassicae		 距离
Distance		 可溶性糖
Soluble sugar (mg g-1)		 可溶性蛋白质
Soluble protein (mg g-1)		 酸溶木质素
Acid soluble lignin (%)		 酸不溶木质素
Acid insoluble lignin (%)
华双5R
Huashuang 5R		 华双5号
Huashuang 5		 华双5R
Huashuang 5R		 华双5号
Huashuang 5		 华双5R
Huashuang 5R		 华双5号
Huashuang 5		 华双5R
Huashuang 5R		 华双5号
Huashuang 5
绩溪2号小种Pathotype2 from Jixi NI 37.01 a 25.60 c 4.87 c 2.67 e 1.86 b 1.26 c 19.57 a 18.99 a
CK 12.45 b 45.98 b 7.16 b 8.90 c 1.17 bc 1.90 bc 16.34 b 17.92 b
T1 12.81 b 26.82 c 3.97 d 4.50 d 1.34 bc 2.00 b 18.41 a 15.80 c
T2 12.13 b 54.64 a 4.15 cd 12.70 b 2.93 a 2.87 a 14.72 c 15.41 c
T3 10.04 c 55.28 a 3.79 d 19.70 a 2.98 a 3.28 a 15.24 c 15.43 c
T4 7.95 d 55.84 a 9.43 a 20.81 a 3.04 a 3.17 a 13.65 d 15.43 c
恩施4号
Pathotype 4 from Enshi		 NI 37.01 a 25.60 d 4.87 d 2.67 c 1.86 b 1.26 de 19.57 a 18.99 a
CK 27.21 b 43.85 a 10.81 a 12.05 a 1.63 b 1.63 cd 17.30 b 18.16 b
T1 20.68 c 17.40 e 6.77 c 2.80 d 1.11 c 2.11 bc 17.60 b 18.13 b
T2 15.07 d 33.26 c 8.63 b 5.30 c 2.76 a 2.79 b 16.08 c 15.21 c
T3 10.91 e 35.40 b 8.99 b 5.06 c 2.75 a 3.56 a 15.78 c 15.93 c
T4 7.01 f 36.78 b 9.30 b 7.49 b 2.90 a 3.74 a 14.43 d 15.45 c

Table 4

Root biochemistry components of Bing 409R and Bing 409 in different treatments"

菌种
P. brassicae		 距离
Distance		 可溶性糖
Soluble sugar (mg g-1)		 可溶性蛋白质
Soluble protein (mg g-1)		 酸溶木质素
Acid soluble lignin (%)		 酸不溶木质素
Acid insoluble lignin (%)
丙409R
Bing 409R		 丙409
Bing 409		 丙409R
Bing 409R		 丙409
Bing 409		 丙409R
Bing 409R		 丙409
Bing 409		 丙409R
Bing 409R		 丙409
Bing 409
礼州2号小种Pathotype 2 from Lizhou NI 33.46 a 35.64 d 3.22 d 8.30 ab 1.87 bc 1.50 d 15.73 a 16.10 a
CK 31.69 b 55.76 a 9.37 a 5.35 c 2.50 ab 3.14 a 14.76 b 13.44 d
T1 16.10 c 25.90 f 6.59 c 8.64 ab 1.32 c 1.61 d 15.83 a 15.00 b
T2 15.83 cd 32.38 e 8.34 b 8.90 a 2.48 ab 3.04 a 14.00 b 13.55 d
T3 14.51 d 43.26 c 8.68 b 9.73 a 2.80 a 2.35 bc 14.43 b 14.98 b
T4 14.07 d 50.71 b 7.13 bc 9.39 a 2.92 a 2.83 ab 10.66c 14.26 c
歙县4号小种Pathotype 4 from Shexian NI 33.46 a 35.64 e 3.22 d 8.30 b 1.87 b 1.50 c 15.73 b 16.10 a
CK 16.82 b 66.57 b 5.23 bc 15.50 a 3.22 a 2.74 ab 15.44 b 15.17 b
T1 16.27 b 39.60 d 3.42 d 8.87 b 1.86 b 1.52 c 16.45 a 14.67 b
T2 15.68 c 48.43 c 5.75 b 4.83 c 3.15 a 3.31 a 13.88 c 12.28 c
T3 12.55 d 49.88 c 11.33 a 3.54 d 3.15 a 2.71 ab 11.09 d 10.79 d
T4 11.50 e 76.25 a 2.99 d 2.54 d 3.44 a 3.21 a 11.57 d 11.83 c

Table 5

Correlation analysis among the root biochemistry components, disease incidence and disease index after inoculation"

指标
Parameter		 可溶性糖
Soluble sugar		 可溶性蛋白质
Soluble protein		 酸溶木质素
Acid soluble lignin		 酸不溶木质素
Acid insoluble lignin		 总木质素
Total lignin
发病率Disease incidence 0.797** 0.403** 0.177 0.089 0.165
病情指数Disease index 0.822** 0.509** 0.176 0.074 0.164
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