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Acta Agronomica Sinica ›› 2023, Vol. 49 ›› Issue (10): 2698-2704.doi: 10.3724/SP.J.1006.2023.24251

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Correlation analysis of sucrose content with protein and oil content and QTL mapping of sucrose content in peanut

GUO Jian-Bin1(), CHENG Liang-Qiang2(), LI Wei-Tao1, LIU Nian1, LUO Huai-Yong1, DING Ying-Bin1, YU Bo-Lun1, CHEN Wei-Gang1, HUANG Li1, ZHOU Xiao-Jing1, LEI Yong1, LIAO Bo-Shou1, JIANG Hui-Fang1()   

  1. 1Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
    2Guizhou Oil Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China
  • Received:2022-11-11 Accepted:2023-02-21 Online:2023-10-12 Published:2023-03-01
  • Contact: E-mail: peanutlab@oilcrops.cn
  • About author:**Contributed equally to this work
  • Supported by:
    Guizhou Provincial Science and Technology Project (QKHJC-ZK[2022]YB 290);Open Project Program of the Key Laboratory of Biology and Genetic Improvement of Oil Crops, the Ministry of Agriculture and Rural Affairs(KF2020008);China Agriculture Research System of MOF and MARA (Peanut, CARS-13-Germplasm Resource Evaluation)

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Abstract:

With the increasing size of edible peanuts market, the quality characteristics of edible peanuts have attracted much attention. Sweetness is an important indicator affecting the flavor and taste of edible peanuts, and it mainly comes from sucrose. Increasing sucrose content is the key to the edible peanuts cultivation, and QTL mapping for sucrose content will lay a foundation for molecular breeding of high sucrose content. However, the increase of sucrose content may affect protein and oil content. In this study, a recombinant inbred line population derived from the cross of Xuhua 13 and Zhonghua 6 was developed. The relationships between sucrose and protein as well as oil content were investigated, and QTLs for sucrose content were mapped. The results showed that sucrose content was not significantly correlated with protein content but there was extremely significantly negatively correlated with the content. Sucrose content presented board phenotypic variations among the RILs, ranging from 14.33 mg g-1 to 61.42 mg g-1. Two lines with high protein, low oil content, and medium sucrose content were observed. Ten QTLs on six chromosomes were detected with 4.56%-12.25% of phenotypic variation explained (PVE) among which qSUCA07 could be repeatedly detected in three environments. The results provide an important information for QTLs fine mapping of sucrose content and cultivating edible peanut varieties with excellent traits.

Key words: peanut, oil content, protein content, sucrose content, quantitative trait locus

Table 1

Correlation coefficients of protein, oil content and sucrose content in the RIL population"

环境 性状 蛋白质 含油量 蔗糖
Environment Trait Protein content Oil content Sucrose content
2020WH 蛋白质 Protein content 1 -0.597** -0.169
含油量 Oil content 1 -0.299**
蔗糖 Sucrose content 1
2021WH 蛋白质 Protein content 1 -0.643** -0.132
含油量 Oil content 1 -0.178*
蔗糖 Sucrose content 1
2021QZ 蛋白质Protein content 1 -0.576** -0.021
含油量 Oil content 1 -0.255**
蔗糖 Sucrose content 1
平均 Mean 蛋白质 Protein content 1 -0.647** -0.077
含油量 Oil content 1 -0.443**
蔗糖 Sucrose content 1

Fig. 1

Parents' difference in sucrose content ** indicates significant difference at the 0.01 probability level. XH13: Xuhua 13; ZH6: Zhonghua 6."

Table 2

Descriptive statistical analysis of sucrose content in the RIL population"

环境
Environment		 最小值
Min.		 最大值
Max.		 均值
Mean		 标准差
SD		 偏度
Skewness		 峰度
Kurtosis		 夏皮洛-威尔克检验
Shapiro-Wilk test W (sig.)
2020WH 16.87 57.93 31.21 9.00 0.74 0.09 0.959 (0.000)
2021WH 14.33 61.42 31.58 8.70 0.81 0.77 0.953 (0.000)
2021QZ 14.49 51.11 32.37 6.49 0.49 0.17 0.978 (0.008)

Fig. 2

Phenotypic distributions of sucrose content in the RIL population in three environments The X-axis and Y-axis represents the sucrose content and the frequency, respectively. Dotted arrow: Zhonghua 6, Solid arrow: Xuhua 13. 2020WH: 2020 Wuhan; 2021WH: 2021 Wuhan; 2021QZ: 2021 Quanzhou. RIL: recombinant inbred lines."

Table 3

Analysis of variance for sucrose content in the RIL population in three environments"

变异来源 自由度 平方和 均方 F值 P值
Source DF SS MS F-value P-value
环境 Environment 2 134.050 67.025 1.576 0.208
基因型 Genotype 185 20,709.541 111.943 2.633 <0.0001
误差 Error 354 15,050.897 42.517
总计 Total 542 581,263.608

Fig. 3

Chromosome-wise logarithm of the odds (LOD) scores of sucrose content QTLs in the RIL population in three environments 2020WH: 2020 Wuhan; 2021WH: 2021 Wuhan; 2021QZ: 2021 Quanzhou. RIL: recombinant inbred lines."

Table 4

QTLs identified for sucrose content in the RIL populations in three environments"

QTL 染色体
Chromosome		 环境
Environment		 位置
Position (cM)		 置信区间
Confidence interval		 LOD值
LOD value		 加性效应
Additive effect		 贡献率
PVE (%)
qSUCA01.1 A01 2020WH 5.11 4.7-7.0 2.62 -1.94 4.60
qSUCA01.2 A01 2020WH 16.01 14.1-17.5 3.00 -2.09 5.23
qSUCA07 A07 2020WH 1.01 0-3.3 6.81 3.21 12.25
2021WH 0.01 0-2.7 4.23 2.49 7.70
2021QZ 0.01 0-3.0 3.51 1.79 7.05
qSUCA10 A10 2020WH 63.70 56.0-64.7 3.60 -2.10 7.14
qSUCB01 B01 2021WH 3.51 0.8-10.9 2.73 -2.06 4.94
qSUCB03.1 B03.1 2020WH 64.81 60.3-66.1 2.70 2.00 4.81
qSUCB10.1 B10 2021QZ 35.71 34.8-37.5 5.47 -2.29 11.45
qSUCB10.2 B10 2021QZ 45.21 43.2-45.6 4.18 -1.98 8.88
qSUCB10.3 B10 2021WH 60.51 59.5-60.7 2.51 -1.95 4.56
qSUCB10.4 B10 2021WH 68.81 67.5-72.7 3.30 -2.22 6.20
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