江苏裸大麦种质资源遗传多样性和群体结构分析

doi:10.3724/SP.J.1006.2023.31006

Abstract

Abstract:

The middle and lower regions of the Yangtze River and the coastal areas were one of the two major production areas of naked barley in China, especially Jiangsu province, which was the largest planting area and was an important production base of naked barley. Studying the genetic diversity and population structure of Jiangsu naked barley germplasm resources is of great significance for broadening the genetic base of existing naked barley resources, mining excellent alleles, and improving the breeding efficiency of naked barley. In this study, a set of 38 simple sequence repeat (SSR) markers were used to assess the genetic diversity of 230 naked barley materials from Jiangsu and 109 materials from seven other sources. 242 alleles were detected with an average allele number of 6.3684 and the effective allele number of 3.092 for each SSR. The mean values of Shannon-Weaver index and Nei were 1.2189 and 0.5857, respectively. The average PIC value was 0.5486, indicating the high polymorphism of the SSRs. When the materials were divided into eight groups according to the regions, the largest number of alleles observed in Jiangsu population was 5.4737 and the mean values of effective alleles, Shannon's diversity index, Nei's diversity index, and PIC were 2.5347, 0.9551, 0.5004, and 0.4593, respectively. The genetic diversity of naked barley materials was mainly caused by genetic variation between and within populations, and gene flow was the main reason for the genetic differentiation among the naked barley materials of different groups. The genetic differentiation coefficient and genetic distance between various groups were not significantly correlated with the geographical location between groups. Based on clustering analysis, population structure analysis, and principal component analysis, the naked barley resources were divided into four groups, and the clustering results could corroborate each other and have not clear correlation with geographical location. The population structure analysis showed that the Jiangsu materials were distributed in all four groups and were clearly differentiated from other groups. In 339 materials, the Q-value of 295 materials were higher than 0.6, and most of them had relatively single genetic background.

Key words: naked barley, SSR primers, Jiangsu, genetic diversity, population structure

LI Ying, LIU Hai-Cui, SHI Lyu, SHI Xiao-Xu, HAN Xiao, LIU Jian, WEI Ya-Feng. Genetic diversity and population structure analysis of naked barley germplasm resources in Jiangsu province[J].Acta Agronomica Sinica, 2023, 49(10): 2687-2697.

Figures/Tables 13

Table S1

Name and origin of 339 naked barley resources used in this study"

序号 No.	统一编号 Uniform No.	名称 Name	产地 Region
1	ZDM2183	红大元麦 Hongdayuanmai	中国江苏沛县 Peixian, Jiangsu, China
2	ZDM2184	白元麦 Baiyuanmai	中国江苏铜山 Tongshan, Jiangsu, China
3	ZDM2185	玉米麦 Yumimai	中国江苏铜山Tongshan, Jiangsu, China
4	ZDM2187	戴帽元麦 Daimaoyuanmai	中国江苏睢宁 Suining, Jiangsu, China
5	ZDM2188	沭阳三月黄 Shuyangsanyuehuang	中国江苏沭阳 Shuyang, Jiangsu, China
6	ZDM2189	青元麦 Qingyuanmai	中国江苏沭阳 Shuyang, Jiangsu, China
7	ZDM2194	白元麦 Baiyuanmai	中国江苏宿迁 Suqian, Jiangsu, China
8	ZDM2204	白元麦 Baiyuanmai	中国江苏滨海 Binhai, Jiangsu, China
9	ZDM2207	建湖三月黄 Jianhusanyuehuang	中国江苏建湖 Jianhu, Jiangsu, China
10	ZDM2209	洋辣子 Yanglazi	中国江苏建湖 Jianhu, Jiangsu, China
11	ZDM2211	盐城紫元麦 Yanchengziyuanmai	中国江苏盐城 Yancheng, Jiangsu, China
12	ZDM2212	四棱元麦 Silengyuanmai	中国江苏东台 Dongtai, Jiangsu, China
13	ZDM2222	太兴四棱 Taixingsileng	中国江苏太兴 Taixing, Jiangsu, China
14	ZDM2228	丹阳三月黄 Danyangsanyuehuang	中国江苏丹阳 Danyang, Jiangsu, China
15	ZDM2232	武进三月黄 Wujinsanyuehuang	中国江苏武进 Wujin, Jiangsu, China
16	ZDM2236	紫元麦 Ziyuanmai	中国江苏灌云 Guanyun, Jiangsu, China
17	ZDM2237	雁口黄 Yankouhuang	中国江苏灌云Guanyun, Jiangsu, China
18	ZDM2240	黄元麦 Huangyuanmai	中国江苏涟水 Lianshui, Jiangsu, China
19	ZDM2241	脱郎头 Tuolangtou	中国江苏涟水Lianshui, Jiangsu, China
20	ZDM2242	六棱元麦 Liulengyuanmai	中国江苏涟水Lianshui, Jiangsu, China
21	ZDM2243	团穗青朗头 Tuansuiqinglangtou	中国江苏涟水Lianshui, Jiangsu, China
22	ZDM2248	六棱元麦 Liulengyuanmai	中国江苏泗阳 Siyang, Jiangsu, China
23	ZDM2249	六棱孔麦 Liulengyuanmai	中国江苏泗阳Siyang, Jiangsu, China
24	ZDM2251	淮安狗尾 Huaiangouwei	中国江苏淮安 Huaian, Jiangsu, China
25	ZDM2251	洋腊元麦 Yanglayuanmai	中国江苏滨海 Binhai, Jiangsu, China
26	ZDM2254	狮子头 Shizitou	中国江苏射阳 Sheyang, Jiangsu, China
27	ZDM2258	洋辣子 Yanglazi	中国江苏建湖 Jianhu, Jiangsu, China
28	ZDM2259	建湖团六棱子 Jianhutuanliulengzi	中国江苏建湖 Jianhu, Jiangsu, China
29	ZDM2261	紫元麦 Ziyuanmai	中国江苏建湖 Jianhu, Jiangsu, China
30	ZDM2263	洋元麦 Yangyuanmai	中国江苏盐城 Yancheng, Jiangsu, China
31	ZDM2265	黑元麦 Heiyuanmai	中国江苏盐城 Yancheng, Jiangsu, China
32	ZDM2266	长六棱白壳 Changliulengbaike	中国江苏如皋 Rugao, Jiangsu, China
33	ZDM2270	紫秆六棱 Ziganliuleng	中国江苏南通 Nantong, Jiangsu, China
34	ZDM2271	南通倒八担 Nantongdaobadan	中国江苏南通 Nantong, Jiangsu, China
35	ZDM2274	南通倒八担 Nantongdaobadan	中国江苏南通 Nantong, Jiangsu, China
36	ZDM2275	南通白麦 Nantongbaimai	中国江苏南通 Nantong, Jiangsu, China
37	ZDM2276	南通冲天炮 Nantongchongtianpao	中国江苏南通 Nantong, Jiangsu, China
38	ZDM2279	南通铁麦 Nantongtiemai	中国江苏南通 Nantong, Jiangsu, China
39	ZDM2280	垂头六棱元麦 Chuitouliulengyuanmai	中国江苏南通 Nantong, Jiangsu, China
40	ZDM2282	如东早六棱头 Rudongzaoliulengtou	中国江苏如东 Rudong, Jiangsu, China
41	ZDM2283	海门火烧六柱头 Haimenhuoshaoliuzhutou	中国江苏海门 Haimen, Jiangsu, China
42	ZDM2284	海门火烧黑柱头 Haimenhuoshaoheizhutou	中国江苏海门 Haimen, Jiangsu, China
43	ZDM2285	海门白皮六棱头 Haimenbaipiliulengtou	中国江苏海门 Haimen, Jiangsu, China
44	ZDM2287	江都四棱子 Jiangdusilengzi	中国江苏江都 Jiangdu, Jiangsu, China
45	ZDM2289	太县洋六棱 Taixianyangliuleng	中国江苏太县 Taixian, Jiangsu, China
46	ZDM2290	太县洋六棱 Taixianyangliuleng	中国江苏太县 Taixian, Jiangsu, China
47	ZDM2291	白六棱 Bailiuleng	中国江苏靖江 Jingjiang, Jiangsu, China
48	ZDM2293	靖江晏来红 Jingjiangyanlaihong	中国江苏靖江 Jingjiang, Jiangsu, China
49	ZDM2295	句容六棱元麦Jurongliulengyuanmai	中国江苏句容 Jurong, Jiangsu, China
50	ZDM2296	扬中红 Yangzhonghong	中国江苏扬中 Yangzhong, Jiangsu, China
51	ZDM2298	丹阳长穗晚元麦 Danyangchangsuiwanyuanmai	中国江苏丹阳 Danyang, Jiangsu, China
52	ZDM2300	宜兴洋元麦 Yixingyangyuanmai	中国江苏宜兴 Yixing, Jiangsu, China
53	ZDM2301	武进晚粒红 Wujinwanlihong	中国江苏武进 Wujin, Jiangsu, China
54	ZDM2302	武进元麦 Wujinyuanmai	中国江苏武进 Wujin, Jiangsu, China
55	ZDM2303	武进四月黄团穗头 Wujinsiyuahuangtuansuitou	中国江苏武进 Wujin, Jiangsu, China
56	ZDM2304	武进六棱头 Wujinliulengtou	中国江苏武进 Wujin, Jiangsu, China
57	ZDM2306	太仓六柱头 Taicangliuzhutou	中国江苏太仓 Taicang, Jiangsu, China
58	ZDM2307	太仓四柱头 Taicangsizhutou	中国江苏太仓 Taicang, Jiangsu, China
59	ZDM2308	江阴六柱头 Jiangyinliuzhutou	中国江苏江阴 Jiangyin, Jiangsu, China
60	ZDM2310	常熟矮脚早裸麦 Changsuaijiaozaoluomai	中国江苏常熟 Changshu, Jiangsu, China
61	ZDM2311	键米大麦 Jianmidamai	中国江苏常熟 Changshu, Jiangsu, China
62	ZDM2313	常熟六棱元麦 Changsuiliulengyuanmai	中国江苏常熟 Changshu, Jiangsu, China
63	ZDM2314	常熟缠茎六柱 Changshuchanjingliuzhu	中国江苏常熟 Changshu, Jiangsu, China
64	ZDM2316	立新2号 Lixin 2	中国江苏大丰 Dafeng, Jiangsu, China
65	ZDM2318	通麦3号 Tongmai 3	中国江苏如皋 Rugao, Jiangsu, China
66	ZDM2320	早芒2号 Zaomang 2	中国江苏如皋 Rugao, Jiangsu, China
67	ZDM2321	海麦1号 Haimai 1	中国江苏海门 Haimen, Jiangsu, China
68	ZDM2322	8023白壳 Baike 8023	中国江苏 Jiangsu, China
69	ZDM2323	村农2号 Cunnong 2	中国江苏 Jiangsu, China
70	ZDM8505	江苏元麦108号 Jiangsuyuanmai 108	中国江苏 Jiangsu, China
71	ZDM8506	江苏元麦109号 Jiangsuyuanmai 109	中国江苏 Jiangsu, China
72	ZDM8508	江苏元麦110号 Jiangsuyuanmai 110	中国江苏 Jiangsu, China
73	ZDM8511	江苏元麦113号 Jiangsuyuanmai 113	中国江苏 Jiangsu, China
74	ZDM8514	江苏元麦116号 Jiangsuyuanmai 116	中国江苏 Jiangsu, China
75	ZDM8515	江苏元麦117号 Jiangsuyuanmai 117	中国江苏 Jiangsu, China
76	ZDM8518	江苏元麦11号 Jiangsuyuanmai 11	中国江苏 Jiangsu, China
77	ZDM8520	江苏元麦121号 Jiangsuyuanmai 121	中国江苏 Jiangsu, China
78	ZDM8522	江苏元麦123号 Jiangsuyuanmai 123	中国江苏 Jiangsu, China
79	ZDM8523	江苏元麦124号 Jiangsuyuanmai 124	中国江苏 Jiangsu, China
80	ZDM8524	江苏元麦125号 Jiangsuyuanmai 125	中国江苏 Jiangsu, China
81	ZDM8525	江苏元麦126号 Jiangsuyuanmai 126	中国江苏 Jiangsu, China
82	ZDM8534	江苏元麦134号 Jiangsuyuanmai 134	中国江苏 Jiangsu, China
83	ZDM8535	江苏元麦135号 Jiangsuyuanmai 135	中国江苏 Jiangsu, China
84	ZDM8536	江苏元麦136号 Jiangsuyuanmai 136	中国江苏 Jiangsu, China
85	ZDM8537	江苏元麦137号 Jiangsuyuanmai 137	中国江苏 Jiangsu, China
86	ZDM8538	江苏元麦138号 Jiangsuyuanmai 138	中国江苏 Jiangsu, China
87	ZDM8540	江苏元麦13号 Jiangsuyuanmai 13	中国江苏 Jiangsu, China
88	ZDM8541	江苏元麦140号 Jiangsuyuanmai 140	中国江苏 Jiangsu, China
89	ZDM8542	江苏元麦141号 Jiangsuyuanmai 141	中国江苏 Jiangsu, China
90	ZDM8543	江苏元麦142号 Jiangsuyuanmai 142	中国江苏 Jiangsu, China
91	ZDM8544	江苏元麦143号 Jiangsuyuanmai 143	中国江苏 Jiangsu, China
92	ZDM8545	江苏元麦144号 Jiangsuyuanmai 144	中国江苏 Jiangsu, China
93	ZDM8546	江苏元麦145号 Jiangsuyuanmai 145	中国江苏 Jiangsu, China
94	ZDM8547	江苏元麦146号 Jiangsuyuanmai 146	中国江苏 Jiangsu, China
95	ZDM8548	江苏元麦147号 Jiangsuyuanmai 147	中国江苏 Jiangsu, China
96	ZDM8549	江苏元麦148号 Jiangsuyuanmai 148	中国江苏 Jiangsu, China
97	ZDM8550	江苏元麦149号 Jiangsuyuanmai 149	中国江苏 Jiangsu, China
98	ZDM8553	江苏元麦151号 Jiangsuyuanmai 151	中国江苏 Jiangsu, China
99	ZDM8554	江苏元麦152号 Jiangsuyuanmai 152	中国江苏 Jiangsu, China
100	ZDM8555	江苏元麦153号 Jiangsuyuanmai 153	中国江苏 Jiangsu, China
101	ZDM8556	江苏元麦154号 Jiangsuyuanmai 154	中国江苏 Jiangsu, China
102	ZDM8557	江苏元麦155号 Jiangsuyuanmai 155	中国江苏 Jiangsu, China
103	ZDM8558	江苏元麦156号 Jiangsuyuanmai 156	中国江苏 Jiangsu, China
104	ZDM8559	江苏元麦157号 Jiangsuyuanmai 157	中国江苏 Jiangsu, China
105	ZDM8560	江苏元麦158号 Jiangsuyuanmai 158	中国江苏 Jiangsu, China
106	ZDM8561	江苏元麦159号 Jiangsuyuanmai 159	中国江苏 Jiangsu, China
107	ZDM8562	江苏元麦15号 Jiangsuyuanmai 15	中国江苏 Jiangsu, China
108	ZDM8563	江苏元麦160号 Jiangsuyuanmai 160	中国江苏 Jiangsu, China
109	ZDM8564	江苏元麦161号 Jiangsuyuanmai 161	中国江苏 Jiangsu, China
110	ZDM8565	江苏元麦162号 Jiangsuyuanmai 162	中国江苏 Jiangsu, China
111	ZDM8566	江苏元麦163号 Jiangsuyuanmai 163	中国江苏 Jiangsu, China
112	ZDM8567	江苏元麦164号 Jiangsuyuanmai 164	中国江苏 Jiangsu, China
113	ZDM8568	江苏元麦165号 Jiangsuyuanmai 165	中国江苏 Jiangsu, China
114	ZDM8569	江苏元麦166号 Jiangsuyuanmai 166	中国江苏 Jiangsu, China
115	ZDM8570	江苏元麦167号 Jiangsuyuanmai 167	中国江苏 Jiangsu, China
116	ZDM8572	江苏元麦17号 Jiangsuyuanmai 17	中国江苏 Jiangsu, China
117	ZDM8573	江苏元麦18号 Jiangsuyuanmai 18	中国江苏 Jiangsu, China
118	ZDM8574	江苏元麦19号 Jiangsuyuanmai 19	中国江苏 Jiangsu, China
119	ZDM8575	江苏元麦1号 Jiangsuyuanmai 1	中国江苏 Jiangsu, China
120	ZDM8576	江苏元麦20号 Jiangsuyuanmai 20	中国江苏 Jiangsu, China
121	ZDM8577	江苏元麦21号 Jiangsuyuanmai 21	中国江苏 Jiangsu, China
122	ZDM8578	江苏元麦22号 Jiangsuyuanmai 22	中国江苏 Jiangsu, China
123	ZDM8579	江苏元麦23号 Jiangsuyuanmai 23	中国江苏 Jiangsu, China
124	ZDM8580	江苏元麦24号 Jiangsuyuanmai 24	中国江苏 Jiangsu, China
125	ZDM8581	江苏元麦25号 Jiangsuyuanmai 25	中国江苏 Jiangsu, China
126	ZDM8582	江苏元麦26号 Jiangsuyuanmai 26	中国江苏 Jiangsu, China
127	ZDM8583	江苏元麦27号 Jiangsuyuanmai 27	中国江苏 Jiangsu, China
128	ZDM8584	江苏元麦28号 Jiangsuyuanmai 28	中国江苏 Jiangsu, China
129	ZDM8585	江苏元麦29号 Jiangsuyuanmai 29	中国江苏 Jiangsu, China
130	ZDM8586	江苏元麦2号 Jiangsuyuanmai 2	中国江苏 Jiangsu, China
131	ZDM8587	江苏元麦30号 Jiangsuyuanmai 30	中国江苏 Jiangsu, China
132	ZDM8588	江苏元麦31号 Jiangsuyuanmai 31	中国江苏 Jiangsu, China
133	ZDM8589	江苏元麦32号 Jiangsuyuanmai 32	中国江苏 Jiangsu, China
134	ZDM8590	江苏元麦33号 Jiangsuyuanmai 33	中国江苏 Jiangsu, China
135	ZDM8591	江苏元麦34号 Jiangsuyuanmai 34	中国江苏 Jiangsu, China
136	ZDM8592	江苏元麦35号 Jiangsuyuanmai 35	中国江苏 Jiangsu, China
137	ZDM8593	江苏元麦36号 Jiangsuyuanmai 36	中国江苏 Jiangsu, China
138	ZDM8594	江苏元麦37号 Jiangsuyuanmai 37	中国江苏 Jiangsu, China
139	ZDM8595	江苏元麦38号 Jiangsuyuanmai 38	中国江苏 Jiangsu, China
140	ZDM8596	江苏元麦39号 Jiangsuyuanmai 39	中国江苏 Jiangsu, China
141	ZDM8597	江苏元麦3号 Jiangsuyuanmai 3	中国江苏 Jiangsu, China
142	ZDM8598	江苏元麦40号 Jiangsuyuanmai 40	中国江苏 Jiangsu, China
143	ZDM8599	江苏元麦41号 Jiangsuyuanmai 41	中国江苏 Jiangsu, China
144	ZDM8600	江苏元麦42号 Jiangsuyuanmai 42	中国江苏 Jiangsu, China
145	ZDM8601	江苏元麦43号 Jiangsuyuanmai 43	中国江苏 Jiangsu, China
146	ZDM8602	江苏元麦44号 Jiangsuyuanmai 44	中国江苏 Jiangsu, China
147	ZDM8603	江苏元麦45号 Jiangsuyuanmai 45	中国江苏 Jiangsu, China
148	ZDM8604	江苏元麦46号 Jiangsuyuanmai 46	中国江苏 Jiangsu, China
149	ZDM8605	江苏元麦47号 Jiangsuyuanmai 47	中国江苏 Jiangsu, China
150	ZDM8606	江苏元麦48号 Jiangsuyuanmai 48	中国江苏 Jiangsu, China
151	ZDM8607	江苏元麦49号 Jiangsuyuanmai 49	中国江苏 Jiangsu, China
152	ZDM8608	江苏元麦4号 Jiangsuyuanmai 4	中国江苏 Jiangsu, China
153	ZDM8609	江苏元麦50号 Jiangsuyuanmai 50	中国江苏 Jiangsu, China
154	ZDM8610	江苏元麦51号 Jiangsuyuanmai 51	中国江苏 Jiangsu, China
155	ZDM8612	江苏元麦53号 Jiangsuyuanmai 53	中国江苏 Jiangsu, China
156	ZDM8613	江苏元麦54号 Jiangsuyuanmai 54	中国江苏 Jiangsu, China
157	ZDM8614	江苏元麦55号 Jiangsuyuanmai 55	中国江苏 Jiangsu, China
158	ZDM8615	江苏元麦56号 Jiangsuyuanmai 56	中国江苏 Jiangsu, China
159	ZDM8616	江苏元麦57号 Jiangsuyuanmai 57	中国江苏 Jiangsu, China
160	ZDM8617	江苏元麦58号 Jiangsuyuanmai 58	中国江苏 Jiangsu, China
161	ZDM8618	江苏元麦59号 Jiangsuyuanmai 59	中国江苏 Jiangsu, China
162	ZDM8619	江苏元麦5号 Jiangsuyuanmai 5	中国江苏 Jiangsu, China
163	ZDM8620	江苏元麦60号 Jiangsuyuanmai 60	中国江苏 Jiangsu, China
164	ZDM8621	江苏元麦61号 Jiangsuyuanmai 61	中国江苏 Jiangsu, China
165	ZDM8622	江苏元麦62号 Jiangsuyuanmai 62	中国江苏 Jiangsu, China
166	ZDM8623	江苏元麦63号 Jiangsuyuanmai 63	中国江苏 Jiangsu, China
167	ZDM8624	江苏元麦64号 Jiangsuyuanmai 64	中国江苏 Jiangsu, China
168	ZDM8625	江苏元麦65号 Jiangsuyuanmai 65	中国江苏 Jiangsu, China
169	ZDM8627	江苏元麦67号 Jiangsuyuanmai 67	中国江苏 Jiangsu, China
170	ZDM8630	江苏元麦6号 Jiangsuyuanmai 6	中国江苏 Jiangsu, China
171	ZDM8635	江苏元麦74号 Jiangsuyuanmai 74	中国江苏 Jiangsu, China
172	ZDM8636	江苏元麦75号 Jiangsuyuanmai 75	中国江苏 Jiangsu, China
173	ZDM8637	江苏元麦76号 Jiangsuyuanmai 76	中国江苏 Jiangsu, China
174	ZDM8638	江苏元麦77号 Jiangsuyuanmai 77	中国江苏 Jiangsu, China
175	ZDM8639	江苏元麦78号 Jiangsuyuanmai 78	中国江苏 Jiangsu, China
176	ZDM8640	江苏元麦79号 Jiangsuyuanmai 79	中国江苏 Jiangsu, China
177	ZDM8641	江苏元麦7号 Jiangsuyuanmai 7	中国江苏 Jiangsu, China
178	ZDM8642	江苏元麦80号 Jiangsuyuanmai 80	中国江苏 Jiangsu, China
179	ZDM8643	江苏元麦81号 Jiangsuyuanmai 81	中国江苏 Jiangsu, China
180	ZDM8645	江苏元麦83号 Jiangsuyuanmai 83	中国江苏 Jiangsu, China
181	ZDM8646	江苏元麦84号 Jiangsuyuanmai 84	中国江苏 Jiangsu, China
182	ZDM8647	江苏元麦85号 Jiangsuyuanmai 85	中国江苏 Jiangsu, China
183	ZDM8648	江苏元麦86号 Jiangsuyuanmai 86	中国江苏 Jiangsu, China
184	ZDM8649	江苏元麦87号 Jiangsuyuanmai 87	中国江苏 Jiangsu, China
185	ZDM8650	江苏元麦88号 Jiangsuyuanmai 88	中国江苏 Jiangsu, China
186	ZDM8651	江苏元麦89号 Jiangsuyuanmai 89	中国江苏 Jiangsu, China
187	ZDM8652	江苏元麦8号 Jiangsuyuanmai 8	中国江苏 Jiangsu, China
188	ZDM8653	江苏元麦90号 Jiangsuyuanmai 90	中国江苏 Jiangsu, China
189	ZDM8654	江苏元麦91号 Jiangsuyuanmai 91	中国江苏 Jiangsu, China
190	ZDM8655	江苏元麦92号 Jiangsuyuanmai 92	中国江苏 Jiangsu, China
191	ZDM8656	江苏元麦93号 Jiangsuyuanmai 93	中国江苏 Jiangsu, China
192	ZDM8657	江苏元麦94号 Jiangsuyuanmai 94	中国江苏 Jiangsu, China
193	ZDM8658	江苏元麦95号 Jiangsuyuanmai 95	中国江苏 Jiangsu, China
194	ZDM8659	江苏元麦96号 Jiangsuyuanmai 96	中国江苏 Jiangsu, China
195	ZDM8660	江苏元麦97号 Jiangsuyuanmai 97	中国江苏 Jiangsu, China
196	ZDM8661	江苏元麦98号 Jiangsuyuanmai 98	中国江苏 Jiangsu, China
197	ZDM8662	江苏元麦99号 Jiangsuyuanmai 99	中国江苏 Jiangsu, China
198	ZDM8663	江苏元麦9号 Jiangsuyuanmai 9	中国江苏 Jiangsu, China
199	ZDM09357	苏511元麦 Suyuanmai 511	中国江苏 Jiangsu, China
200	ZDM09450	苏728 Su728	中国江苏 Jiangsu, China
201	ZDM09455	苏71-27-1 Su71-27-1	中国江苏 Jiangsu, China
202	ZDM09462	苏71-43-2 Su71-43-2	中国江苏 Jiangsu, China
203	ZDM09463	苏71-43-8 Su71-43-8	中国江苏 Jiangsu, China
204	ZDM09464	苏71-58 Su71-58	中国江苏 Jiangsu, China
205	ZDM09465	苏71-59-34 Su71-59-34	中国江苏 Jiangsu, China
206	ZDM09466	苏71-75-12 Su 71-75-12	中国江苏 Jiangsu, China
207	ZDM09467	苏71-84 Su71-84	中国江苏 Jiangsu, China
208	ZDM09468	苏72-18-19(22) Su72-18-19(22)	中国江苏 Jiangsu, China
209	ZDM09469	苏72-28 Su72-28	中国江苏 Jiangsu, China
210	ZDM09471	苏72-37 Su72-37	中国江苏 Jiangsu, China
211	ZDM09472	苏72-38(9)Su72-38(9)	中国江苏 Jiangsu, China
212	ZDM09473	苏72-39-(2)Su72-39-(2)	中国江苏 Jiangsu, China
213	ZDM09474	苏72-41-3 Su72-41-3	中国江苏 Jiangsu, China
214	ZDM09475	苏72-44-(48)Su72-44-(48)	中国江苏 Jiangsu, China
215	ZDM09478	苏72-49 Su72-49	中国江苏 Jiangsu, China
216	ZDM09479	苏72-63 Su72-63	中国江苏 Jiangsu, China
217	ZDM09480	苏76-39 Su 76-39	中国江苏 Jiangsu, China
218	ZDM09481	苏785 Su 785	中国江苏 Jiangsu, China
219		昆仑12 Kunlun 12	中国青海 Qinghai, China
220		昆仑13 Kunlun 13	中国青海 Qinghai, China
221		昆仑14 Kunlun 14	中国青海 Qinghai, China
222		昆仑15 Kunlun 15	中国青海 Qinghai, China
223		柴青1号 Chaiqing 1	中国青海 Qinghai, China
224		21DJ1	中国江苏 Jiangsu, China
225		21DJ2	中国江苏 Jiangsu, China
226		21DJ3	中国江苏 Jiangsu, China
227		21DJ7	中国江苏 Jiangsu, China
228		21DJ8	中国江苏 Jiangsu, China
229		21DJ9	中国江苏 Jiangsu, China
230		甘垦5号 Ganken 5	中国甘肃 Gansu, China
231		苏裸麦2号 Suluomai 2	中国江苏 Jiangsu, China
232		通0914 Tong 0914	中国江苏 Jiangsu, China
233		紫元麦 Ziyuanmai	中国西藏 Tibet, China
234		肚里黄 Dulihuang	中国青海 Qinghai, China
235		5171	中国西藏 Tibet, China
236		甘青4号 Ganqing 4	中国青海 Qinghai, China
237		东陈元麦 Dongchenyuanmai	中国江苏 Jiangsu, China
238		青元麦 Qingyuanmai	中国江苏 Jiangsu, China
239		泰兴元麦 Taixingyuanmai	中国江苏 Jiangsu, China
240	ZDM00006	罗道庄大麦 Luodaozhuangdamai	中国北京 Beijing, China
241	ZDM03261	临海裸大麦Linhailuodamai	中国浙江 Zhejiang, China
242	ZDM06853	勒清 Leqing	中国西藏 Tibet, China
243	ZDM07528	尼泊尔青稞 Niboerqingke	尼泊尔 Nepal
244	ZDM07534	皮当蓝 Pidanglan	中国西藏 Tibet, China
245	ZDM07554	西嘎果那 Xigaguona	中国西藏 Tibet, China
246	ZDM07562	曲布蓝 Qubulan	中国西藏 Tibet, China
247	ZDM07571	竹居青 Zujuqing	中国西藏 Tibet, China
248	ZDM07573	色堆短芒 Seduiduanmang	中国西藏 Tibet, China
249	ZDM07610	沙尔兰 Shaerlan	中国西藏 Tibet, China
250	ZDM07612	沃马紫 Womazi	中国西藏 Tibet, China
251	ZDM07613	沃马兰 Womalan	中国西藏 Tibet, China
252	ZDM07635	油松 Yousong	中国西藏 Tibet, China
253	ZDM07646	定结牧紫 Dingjiemuzi	中国西藏 Tibet, China
254	ZDM07648	孟德黄 Mengdehuang	中国西藏 Tibet, China
255	ZDM07651	春丕青稞 Chunpeiqingke	中国西藏 Tibet, China
256	ZDM07738	雄马兰 Xiongmalan	中国西藏 Tibet, China
257	ZDM07746	短芒青稞 Duanmangqingke	中国西藏 Tibet, China
258	ZDM07747	稀穗短芒 Xisuiduanmang	中国西藏 Tibet, China
259	ZDM08086	白青稞 baiqingke	中国青海 Qinghai, China
260	ZDM08108	四棱黑青稞 Silengheiqingke	中国青海 Qinghai, China
261	ZDM08141	长芒黑青稞 Changmangheiqingke	中国青海 Qinghai, China
262	ZDM08177	红胶泥 Hongjiaoni	中国青海 Qinghai, China
263	ZDM08193	钩芒紫青稞 Goumangziqingke	中国青海 Qinghai, China
264	ZDM08205	紫青稞 Ziqingke	中国青海 Qinghai, China
265	ZDM08217	黑青稞 Heiqingke	中国青海 Qinghai, China
266	ZDM08227	蓝青稞 Lanqingke	中国青海 Qinghai, China
267	ZDM08706	阿黑 Ahei	中国四川 Sichuan, China
268	ZDM08751	色黑瑞朗 Seheiruilang	中国四川 Sichuan, China
269	ZDM08784	日大多 Ridaduo	中国四川 Sichuan, China
270	ZDM08790	泽盖紫青稞 Zegaiziqingke	中国四川 Sichuan, China
271	ZDM08811	足木足大头六棱 Zumuzudatouliuleng	中国四川 Sichuan, China
272	ZDM08841	小青稞 Xiaoqingke	中国四川 Sichuan, China
273	ZDM08846	荞窝青稞 Qiaowoqingke	中国四川 Sichuan, China
274	ZDM09082	长钩紫大麦 Changgouzidamai	中国云南 Yunnan, China
275	ZDM09113	光芒黄大麦 Guangmanghuangdamai	中国云南 Yunnan, China
276	ZDM09369	苏农370 Sunong 370	中国江苏 Jiangsu, China
277	ZDM09653	阿212 A 212	中国四川 Sichuan, China
278	ZDM09748	藏830187 Zang830187	中国西藏 Tibet, China
279	ZDM09767	藏830754 Zang830754	中国西藏 Tibet, China
280	ZDM09772	藏830928 Zang830928	中国西藏 Tibet, China
281	ZDM09777	藏831247 Zang831247	中国西藏 Tibet, China
282	ZDM09779	藏831339 Zang831339	中国西藏 Tibet, China
283	ZDM09802	藏840714 Zang840714	中国西藏 Tibet, China
284	ZDM09817	藏85品3872 Zang85Pin3872	中国西藏 Tibet, China
285	ZDM09824	藏861228 Zang861228	中国西藏 Tibet, China
286	WDM00772	Jet	丹麦 Denmark
287	WDM02118	H.tetrast.L.V.viol	捷克斯洛伐克 Czechoslovakia
288	WDM02131	H.Viol B.8c H	丹麦 Denmark
289	WDM02972	Bonanza	加拿大 Canada
290	WDM03202	83	加拿大 Canada
291	WDM03203	84	加拿大 Canada
292	WDM03227	Black Hulless	丹麦 Denmark
293	WDM03228	Black Hull-less C.I.596	美国 America
294	WDM03230	Black Hull-less C.I.1027	美国 America
295	WDM03233	Black Hull-less C.I.2320	美国 America
296	WDM03237	Brachiki	墨西哥 Mexico
297	WDM03238	C1334	加拿大 Canada
298	WDM03263	Gaffel byg nφgen	丹麦 Denmark
299	WDM03279	H.polyst.V.trif.C	丹麦 Denmark
300	WDM03280	H.riol.B.Sch	丹麦 Denmark
301	WDM03315	Lyallpur	丹麦 Denmark
302	WDM03361	SIBIRI"S"	墨西哥 Mexico
303	WDM03362	SNJCO"S"	墨西哥 Mexico
304	WDM03390	C 2734	加拿大 Canada
305	WDM03406	MANKER-OREGANO	墨西哥 Mexico
306	WDM03411	OREGANO	墨西哥 Mexico
307	WDM03419	Streng	叙利亚 Syria
308	WDM03846	C1264	加拿大 Canada
309	WDM04080	CMB-74A-1796-B-6B-1Y-1B-1Y-1B-OY	墨西哥 Mexico
310	WDM04276	CMB76-839-C-500Y-500B-501Y-503B-500Y-OB	墨西哥 Mexico
311	WDM05243	Japanische419	西德 West Germany
312	WDM05418	Muanbori	日本 Japan
313	WDM05675	Scout	加拿大 Canada
314	WDM06222	四国裸46号 Siguoluo46	日本 Japan
315	WDM06223	四国裸86号 Siguoluo86	日本 Japan
316	WDM06224	四国裸87号 Siguoluo87	日本 Japan
317	WDM06225	四国裸88号 Siguoluo88	日本 Japan
318	WDM06226	四国裸89号 Siguoluo89	日本 Japan
319	WDM06256	御岛裸 Yudaoluo	日本 Japan
320	WDM06275	III-134	日本 Japan
321	WDM06281	III-526	日本 Japan
322	WDM06288	III-558	日本 Japan
323	WDM06289	III-564	日本 Japan
324	WDM06290	III-570	日本 Japan
325	WDM06306	Ⅴ-193	日本 Japan
326	WDM06311	Ⅵ-109	日本 Japan
327	WDM06316	Ⅶ-131	日本 Japan
328		9H88-009	中国西藏 Tibet, China
329		藏青690 Zangqing 690	中国西藏 Tibet, China
330		冬青18 Dongqing 18	中国西藏 Tibet, China
331		2016-品比36 2016-pinbi36	中国西藏 Tibet, China
332		喜马拉雅22 Ximalaya22	中国西藏 Tibet, China
333		藏青25 Zanqging25	中国西藏 Tibet, China
334		09-5024-3	中国西藏 Tibet, China
335		喜马拉雅19 Ximalaya19	中国西藏 Tibet, China
336		12-10-556	中国西藏 Tibet, China
337		08-1128	中国西藏 Tibet, China
338		08-1127	中国西藏 Tibet, China
339		2016-品比42 2016-pinbi-42	中国西藏 Tibet, China

Table S1

Table S2

Information of SSR markers"

引物 Primer	上游引物 Forward primer (5'-3')	下游引物 Reverse primer (5'-3')	染色体 Chromosome	位置 Position (cM)	退火温度 Annealing temperature (℃)
scssr04163	GAAGAAACAACCCAACTTCC	AGGATCGTACGAAGAACAGC	1H	129.7	56
Bmag0579	CCTAGATAAGGAACATAGCCA	CAAAGACCCTAACTCATGTTC	1H	127	56
AWBMS0080	GCTTTCAGCATCTCAACTGCG	TGTATGCCAGGAAAACGGAACC	1H	59.1	56
Bmac0090	ACATCAACCCTCCTGCTC	CCGCACATAGTGGTTACATC	1H	52.3	58
Bmac32	CCATCAAAGTCCGGCTAG	GTCGGGCCTCATACTGAC	1H	105	58
HVM0054	AACCCAGTAACACCGTCCTG	AGTTCCCTGACCCGATGTC	2H	122.41	58
Bmag0125	AATTAGCGAGAACAAAATCAC	AGATAACGATGCACCACC	2H	89.83	56
scssr03381	CAGGGACGTACAATATTACCG	AATAAGCAGGGTACCTTTTGG	2H	64.9	56
Bmag0692	GCAAGGTATCTCTTGTATTTTG	GGCATCTACAATCTAAAACA	2H	46.23	56
EBmag0793	ATATATCAGCTCGGTCTCTCA	AACATAGTAGAGGCGTAGGTG	2H	177	60
Bmag0013	AAGGGGAATCAAAATGGGAG	TCGAATAGGTCTCCGAAGAAA	3H	104.2	58
Bmag0006	TTAAACCCCCCCCCTCTAG	TGCAGTTACTATCGCTGATTTAGC	3H	41	58
Bmag0828	CATCTCCTAAGGTTTATCTTTGA	AGATCGTGACCTCTCAAGAC	3H	42.1	58
Bmag0225	AACACACCAAAAATATTACATCA	CGAGTAGTTCCCATGTGAC	3H	75.54	56
Bmag0603	ATACCATGATACATCACATCG	GGGGGTATGTACGACTAACTA	3H	46.1	56
Bmac0209	CTAGCAACTTCCCAACCGAC	ATGCCTGTGTGTGGACCAT	3H	52.39	56
HVM67	GTCGGGCTCCATTGCTCT	CCGGTACCCAGTGACGAC	4H	102.5	56
Bmac310	CTACCTCTGAGATATCATGCC	ATCTAGTGTGTGTTGCTTCCT	4H	64.37	58
HVM40	CGATTCCCCTTTTCCCAC	ATTCTCCGCCGTCCACTC	4H	14	58
EBmac0701	ATGATGAGAACTCTTCACCC	TGGCACTAAAGCAAAAGAC	4H	96.17	56
EBmac775	GCTTCCTTCATAGACCCAT	ATATCATGCCAATGGTGTC	4H	80	56
EBmac0679	ATTGGAGCGGATTAGGAT	CCCTATGTCATGTAGGAGATG	4H	101	56
Bmag0323	TTTGTGACATCTCAAGAACAC	TGACAAACAAATAATCACAGG	5H	50.85	56
Bmac0096	GCTATGGCGTACTATGTATGGTTG	TCACGATGAGGTATGATCAAAGA	5H	53.12	56
GMS001	CTGACCCTTTGCTTAACATGC	TCAGCGTGACAAACAATAAAGG	5H	200	56
Bmag223	TTAGTCACCCTCAACGGT	CCCCTAACTGCTGTGATG	5H	86.88	56
GBS0613	AGTGTTACATGCATCGCACCG	GGCTTCATCGTCTACCCTTCG	5H	71.5	56
GBM1215	ATGACCAGAAAACGCCTGTC	GGATTCTGCACACACGAGAA	6H	35.4	56
Bmac0316	ATGGTAGAGGTCCCAACTG	ATCACTGCTGTGCCTAGC	6H	7.16	56
Bmag0870	AACCATAGGATTTGTACTAGTTTC	TCATGACATCTCAAGAACG	6H	50.5	56
Bmag0496	AGTATAACCAACAGCCGTCTA	CTATAGCACGCCTTTGAGA	6H	63.76	56
Ebmac0602	GATTGGAGCTTCGGATCAC	CCGTCTAGGGAGAGGTTCTC	6H	75.42	56
scssr05599	TTCCATCATAACAGCAATGG	TTCGTCGAAGGCTATGTAGG	6H	111	56
Bmag0206	TTTTCCCCTATTATAGTGACG	TAGAACTGGGTATTTCCTTGA	7H	17.9	56
EBmac764	AGAATCAAGATCGACCAAAC	AAAAACATGAACCGATGAA	7H	77	56
scssr04056	CCCATGAAGCCTCTTTACG	GGAACGGAGGGAGTATTAAGC	7H	148.22	56
Bmag120	ATTTCATCCCAAAGGAGAC	GTCACATAGACAGTTGTCTTCC	7H	152	56
Bmag7	TGAAGGAAGAATAAACAACCAACA	TCCCCTATTATAGTGACGGTGTG	7H	27	56

Table S2

Fig. 1

Table 1

Genetic diversity of 38 SSR makers in 339 naked barley germplasm resources"

引物 Primer name	等位基因数Number of alleles (Na)	有效等位基因数 Number of effective alleles (Ne)	Shannon's多样性指数 Shannon's diversity index (I)	Nei's多样性指数 Nei's diversity index (H)	多态性信息含量 Polymorphism information content (PIC)
scssr04163	6	2.0982	1.1011	0.5234	0.4943
Bmag0579	4	1.5063	0.6839	0.3361	0.3161
AWBMS0080	4	2.4089	1.0325	0.5849	0.5125
Bmac0090	6	2.0496	1.0753	0.5121	0.4845
Bmac32	8	4.4001	1.7026	0.7727	0.7435
HVM0054	4	2.6254	1.1396	0.6191	0.5677
Bmag0125	7	3.9375	1.5629	0.7460	0.7097
scssr03381	5	3.0967	1.2437	0.6771	0.6173
Bmag0692	10	6.9871	2.0562	0.8569	0.8403
EBmag0793	6	2.9180	1.2420	0.6573	0.6068
Bmag0013	7	3.3836	1.4324	0.7045	0.6577
Bmag0006	5	3.7984	1.4307	0.7367	0.6915
Bmag0828	11	7.5891	2.1680	0.8682	0.8543
Bmag0225	7	3.9071	1.5401	0.7441	0.7044
Bmag0603	5	1.2531	0.4673	0.2020	0.1950
Bmac0209	5	2.7524	1.1854	0.6367	0.5773
HVM67	7	3.4316	1.4449	0.7086	0.6606
Bmac310	6	2.3389	1.2169	0.5724	0.5445
HVM40	4	1.8514	0.8109	0.4599	0.3989
EBmac0701	6	1.2764	0.5132	0.2165	0.2091
EBmac775	7	2.2267	1.1595	0.5509	0.5208
EBmac0679	6	1.3047	0.5691	0.2335	0.2273
Bmag0323	7	4.7185	1.6742	0.7881	0.7576
Bmac0096	6	2.4094	1.2118	0.5850	0.5549
GMS001	4	1.6010	0.7532	0.3754	0.3520
Bmag223	9	3.4627	1.5768	0.7112	0.6822
GBS0613	2	1.9725	0.6862	0.4930	0.3715
GBM1215	5	1.9051	0.8742	0.4751	0.4154
Bmac0316	5	1.1488	0.3308	0.1295	0.1269
Bmag0870	8	4.8852	1.7903	0.7953	0.7691
Bmag0496	10	7.2859	2.1266	0.8627	0.8483
Ebmac0602	5	1.5542	0.7220	0.3566	0.3334
scssr05599	3	1.3087	0.4580	0.2359	0.2178
Bmag0206	11	5.1443	1.8001	0.8056	0.7780
EBmac764	7	3.4792	1.4905	0.7126	0.6728
scssr04056	9	2.7031	1.2559	0.6301	0.5626
Bmag120	7	2.6230	1.1872	0.6188	0.5467
Bmag7	8	4.1988	1.6034	0.7618	0.7248
平均值Mean	6.3684	3.0932	1.2189	0.5857	0.5486

Table 1

Table 2

Table 3

Table 4

Table 5

Fig. 2

Fig. 3

Fig. 4

Table 6

Fig. 5

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来源 Origin	组群大小 Sample size	等位基因数 Number of alleles (N_a)	有效等位基因数 Number of effective alleles (N_e)	Shannon's多样性指数 Shannon's diversity index (I)	Nei's多样性指数 Nei's diversity index (H)	多态性信息含量 Polymorphism information content (PIC)
中国江苏Jiangsu, China	230	5.4737±2.0366	2.5347±1.3079	0.9951±0.4945	0.5004±0.2333	0.4593
中国西藏Tibet, China	38	3.2105±1.5796	2.3819±1.0639	0.8698±0.5037	0.4807±0.2506	0.5494
中国青海Qinghai, China	15	5.0000±1.6926	2.8656±1.0413	1.1693±0.3860	0.5944±0.1728	0.4339
中国四川Sichuan, China	8	3.0263±0.9722	2.7427±0.9586	0.9924±0.3693	0.5826±0.1799	0.3900
亚洲^#Asia^#	17	3.7105±1.1368	2.8741±1.0355	1.1135±0.3230	0.6111±0.1261	0.5192
欧洲 Europe	9	3.9211±1.5489	2.7827±1.0754	1.0619±0.4499	0.5691±0.2173	0.5559
北美洲 North America	17	4.8158±1.7531	3.3396±1.3056	1.2742±0.4197	0.6459±0.1651	0.6009

群体 Population	等位基因数 Number of alleles (N_a)	有效等位基因数 Number of effective alleles (N_e)	Shannon's多样性指数 Shannon's diversity index (I)	Nei's多样性指数 Nei's diversity index (H)	多态性信息含量 Polymorphism information content (PIC)
国内Domestic	6.1579±2.0603	2.9005 ±1.5723	1.1488±0.4885	0.5576±0.2135	0.5196
国外Abroad	5.6842±2.0547	3.7357 ±1.6537	1.3805±0.4296	0.6718±0.1564	0.6322

群体 Population	中国江苏 Jiangsu, China	中国西藏 Tibet, China	中国青海 Qinghai, China	中国四川 Sichuan, China	亚洲(除中国) Asia (except China)	欧洲 Europe	北美洲 North America
中国江苏 Jiangsu, China	0	0.271	0.316	0.356	0.284	0.360	0.320
中国西藏 Tibet, China	0.083	0	0.182	0.211	0.202	0.241	0.234
中国青海 Qinghai, China	0.113	0.069	0	0.157	0.313	0.258	0.249
中国四川 Sichuan, China	0.132	0.084	0.078	0	0.341	0.318	0.323
亚洲^#Asia^#	0.089	0.059	0.110	0.122	0	0.356	0.315
欧洲 Europe	0.108	0.065	0.092	0.113	0.094	0	0.177
北美洲 North America	0.098	0.061	0.088	0.112	0.085	0.045	0

变异来源 Source	自由度 DF	平方和 SS	均方差 MSE	方差分量 Variance component	方差分量百分率 Total variance (%)	基因流 Gene flow (Nm)
群体间 Among population	7	803.578	114.797	1.881	15.58	—
群体内 In population	331	6720.944	20.305	10.113	83.76	—
个体内 In individual	339	27.000	0.080	0.080	0.66	—
总体 Total	677	7551.522		12.073	100.00	—
平均 Mean	—	—	—	—	—	1.355

Genetic diversity and population structure analysis of naked barley germplasm resources in Jiangsu province

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