欢迎访问作物学报，今天是 2025年7月20日 星期日
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (12): 2192-2197.doi: 10.3724/SP.J.1006.2012.02192

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选

李祥晓1，王倩1，罗生香1，何云霞2，朱苓华1，周永力1,*，黎志康1   

  1. 1 中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程, 北京100081; 2黑龙江省农业科学院生物技术研究所, 黑龙江哈尔滨150086
  • 收稿日期:2012-04-06 修回日期:2012-07-05 出版日期:2012-12-12 网络出版日期:2012-10-08
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目(2006-G51)和比尔-盖茨基金项目资助。

Analyzing Avirulence Genes of Magnaporthe oryzae from Heilongjiang Province and Screening Rice Germplasm with Resistance to Blast Fungus

LI Xiang-Xiao1,WANG Qian1,LUO Sheng-Xiang1,HE Yun-Xia2,ZHU Ling-Hua1,ZHOU Yong-Li1,*,LI Zhi-Kang1   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 2 Institute of Biology Technique, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2012-04-06 Revised:2012-07-05 Published:2012-12-12 Published online:2012-10-08

RichHTML

PDF

2075

被引次数

19

摘要：

采用7个稻瘟病菌无毒基因的特异性引物，对177个来自黑龙江省的稻瘟病菌单孢菌株进行检测结果表明7个无毒基因在黑龙江省不同地区均有分布，但出现频率不同，其中频率最高的为ACE1，达到61.6%，最低的为Avr1-co39，只有31.6%；来源于不同水稻品种的菌株无毒基因组成不同。采用国际水稻研究所20个已知抗性基因的单基因系对48个黑龙江省稻瘟病菌进行毒力分析，与PCR检测无毒基因结果一致。同时，对20个抗病单基因系采用多菌株人工接种发现Pi-9在黑龙江6个地区对稻瘟病菌的抗谱为80%~100%，在育种中具有较高利用价值。

关键词: 稻瘟病菌, 无毒基因, 毒力频率, 抗病性

Abstract:

Theavirulence genes from 177 single spore strains of Magnaporthe grisea isolated from different rice varieties in Heilongjiang province were detected by specific primers. The results indicated that Avr-pita, ACE1, Avr-pia, Avr-pit, Avr1-co39, Avr-pik, and Avr-pizt could be detected in the strains from different rice-growing regions, and the amplification frequencies of avirurence genes were associated with the geographical orgin and rice variety, ACE1 and Avr1-co39 showed the highest and lowest frequency, that was 61.6%and 31.6%, respectively. Moreover, 20 monogenic lines with known blast resistance genes from International Rice Research Institute (IRRI) were used to analyze the genes which were valuable in breeding program in Heilongjiang Province by artificial inoculation. The resistance gene Pi-9 had the broadest resistance spectrum to theisolates from six regions.

Key words: Magnaporthe grisea, Avirulence gene, Virulence frequency, Resistance

[1]Liu J L, Wang X J, Mitchell T, Hu Y J, Liu X L, Dai L Y, Wang G L. Recent progress and understanding of the molecular mechanisms of the rice Magnaporthe oryzae interaction. Mol Plant Pathol, 2010, 11: 419–427



[2]Flor H H. Current status of the gene-for-gene concept. Annu Rev Phytopathol, 1971, 9: 275–296



[3]Lei C-L(雷财林), Wang J-L(王久林), Jiang W-R(蒋婉茹), Ling Z-Z(凌忠专). Study on pathologic races and virulence of blast fungus and their movement in japonica rice-growing region of Northern China. Acta Agron Sin (作物学报), 2000, 26(6): 769–776 (in Chinese with English abstract)



[4]Mekwatanakarn P, Kositratana W, Levy M, Zeigler R S. Pathotype and virulence gene diversity of Pyricularia grisea in Thailand as determined by rice lines near isogenic for major resistance genes. Plant Disease, 2000, 84: 60–70



[5]Sun Q(孙强), Zhang S-Y(张三元), Zhang J-G(张俊国), Yang C-G(杨春刚). Current situation of rice production in Northeast of China and counter measures. North Rice (北方水稻), 2010, 40(2): 72–74 (in Chinese with English abstract)



[6]Lei C-L(雷财林), Zhang G-M(张国民), Cheng Z-J(程治军), Ma J-T(马军滔), Wang J-L(王久林), Xin A-H(辛爱华), Chen P(陈平), Xiao J-L(肖家雷), Zhang X(张欣), Liu Y-X(刘迎雪), Guo X-P(郭秀平), Wang J(王洁), Zhai H-Q(翟虎渠), Wan J-M(万建民). Pathogenic races and virulence gene structure of Magnaporthe oryzae population and rice breeding strategy for blast resistance in Heilongjiang province. Acta Agron Sin (作物学报), 2011, 37(1): 18–27 (in Chinese with English abstract)



[7]Zhang Q-F(张崎峰), Jin X-H(靳学慧), Cai X-X(蔡鑫鑫), Gong S-Y(巩双印), Liu X-Y(刘显元). Identification of Magnaporthe oryzae avirulence gene in Heilongjiang province with SSR marker. Chin Agric Sci Bull (中国农学通报), 2010, 26(10): 250–254 (in Chinese with English abstract)



[8]Liu H-Z(刘华招), Liu Y(刘延), Liu H-L(刘化龙), Xu Z-J(徐正进), Chen W-F(陈温福). Distribution of two blast resistance genes Pib and Pita in major rice cultivars in Heilongjiang province in China. J Northeast Agric Univ (东北农业大学学报), 2011, 42(4): 27–31 (in Chinese with English abstract)



[9]Li C-Y(李成云), Luo C-X(罗朝喜), Li J-B(李进斌), Shen Y(沈瑛), Ise K(伊势一男). Mapping avirulence gene in the rice blast fungus Magnaporthe grisea. Sci Agric Sin (中国农业科学), 2000, 33(3): 49–53 (in Chinese with English abstract)



[10]Shi J(石军), Wang Z(王志), Li S-G(李仕贵), Ma B-T(马炳田). Correlation between pathotypes and DNA fingerprinting patterns based on virulence gene of rice blast fungus. Mol Plant Breed (分子植物育种), 2008, 6(6): 1050–1056 (in Chinese with English abstract)



[11]Zheng X-B(郑晓波),Wang Y-C(王源超), Chen Q-H(陈庆河), Zhang Z-G(张正光). Molecular marker methods of Magnaporthe oryzae avirulence gene. China, 200610096493.9, 2007 (in Chinese)



[12]Wang J-F(王建飞), Bao Y-M(鲍永美), Li P-F(李培富), Zhang H-S(张红生). Primary study on correlation between pathotypes and DNA fingerprintings based on avirulence gene sequence of rice blast fungus Magnaporthe oryzae. Chin J Rice Sci (中国水稻科学), 2006, 20(1): 109–112 (in Chinese with English abstract)



[13]Fukuta Y. Development of differential varieties for blast resistance in IRRI-Japan collaborative research project. In: Kawasaki S ed. Rice Blast: Interaction with Rice and Control. Netherlands: Kluwer Academic Publishers, 2004. pp 229–233



[14]Zhu X-Y(朱小源), Yang Q-Y(杨祈云), Yang J-Y(杨健源), Lei C-L(雷财林), Wang J-L(王久林), Ling Z-Z(凌忠专). Differentiation ability of monogenic lines to Magnaporthe grisea in indica rice. Acta Phytopathol Sin (植物病理学报), 2004, 34(4): 361–368 (in Chinese with English abstract)



[15]Zhou J-H(周江鸿), Wang J-L(王久林), Jiang W-R(蒋琬如), Lei C-L(雷财林), Ling Z-Z(凌忠专). Virulence genes diversity and geographic distribution of Pyricularia grisea in China. Acta Agron Sin (作物学报), 2003, 29(5): 646–651 (in Chinese with English abstract)



[16]Yu S B, Xu W J, Vijayakumar C H M, Ali J, Fu B Y, Xu J L, Jiang Y Z, Marghirang R, Domingo J, Aquino C, Virmani S S, Li Z K. Molecular diversity and multilocus organization of the parental lines used in the international rice molecular breeding program. Theor Appl Genet, 2003, 108: 131–140



[17]Valent B, Khang C H. Recent advances in rice blast effector research. Curr Opin Plant Biol, 2010, 13: 434–441



[18]Yasuda N, Noguchi M T, Fujita Y. Partial mapping of avirulence genes Avr-pii and Avr-pia in the rice blast fungus Magnaporthe oryzae. Can J Plant Pathol, 2006, 28: 494–498



[19]Farman M L, Leong S A. Genetic and physical mapping of temoleres in the rice blast fungus, Magnaporthe oryzae. Genetics, 1995, 140: 479–492



[20]Kaye C, Milazzo J, Rozenfeld S, Lebrun M H, Tharreau D. The development of simple sequence repeat markers for Magnaporthe oryzae and their integration into an established genetic linkage map. Fungal Genet Biol, 2003, 40: 207–214



[21]Zhang L-H(张连洪), Yan J-Y(燕继晔), Zhao W-S(赵文生), Zhang G-Z(张国珍), Guo L-Y(国立耘), Peng Y-L(彭友良). Mapping of avirulence gene AVR-Pi-km in Magnaporthe grisea. Acta Phytopathol Sin (植物病理学报), 2006, 36(2): 116–122 (in Chinese with English abstract)



[22]Ma J-T(马军韬), Zhang G-M(张国民), Xin A-H(辛爱华), Xiao J-L(肖家雷), Liu Y-X(刘迎雪), Gong X-J(宫秀杰). Identification and analysis of the physiologic races of Magnaporthe oryzae in Heilongjiang Province. Plant Prot (植物保护), 2010, 36(3): 97–99 (in Chinese with English abstract)
[1] 傅华英, 张婷, 彭文静, 段瑶瑶, 许哲昕, 林艺华, 高三基. 甘蔗新品种(系)苗期白条病人工接种抗性鉴定与评价[J]. 作物学报, 2021, 47(8): 1531-1539.
[2] 张雪翠, 孙素丽, 卢为国, 李海朝, 贾岩岩, 段灿星, 朱振东. 河南大豆新品系抗大豆疫霉根腐病基因鉴定[J]. 作物学报, 2021, 47(2): 275-284.
[3] 李竹, 许莉萍, 苏亚春, 吴期滨, 成伟, 孙婷婷, 高世武. 基于田间表型和Bru1基因检测分析甘蔗褐锈病抗性遗传[J]. 作物学报, 2018, 44(02): 306-312.
[4] 马军韬，张国民，辛爱华，张丽艳，邓凌韦，王永力，王英，任洋，宫秀杰，葛选良，杨秀峰. 不同遗传背景下稻瘟病菌致病性对比分析[J]. 作物学报, 2015, 41(12): 1791-1801.
[5] 孙喜营,崔磊,孙蕾,孙艳玲,邱丹,邹景伟,武小菲,王晓鸣,李洪杰. 抗禾谷孢囊线虫小麦新种质H3714和H4058的培育与鉴定[J]. 作物学报, 2015, 41(06): 872-880.
[6] 李继发，邓志英，孙福来，关西贞，王延训，田纪春. 小麦新品种“山农20”抗病基因的分子检测[J]. 作物学报, 2014, 40(04): 611-621.
[7] 李洪杰,王晓鸣,陈怀谷,李伟,刘东涛,张会云. 小麦-偃麦草杂种后代及小麦种质资源对纹枯病的抗性[J]. 作物学报, 2013, 39(06): 999-1012.
[8] 代君丽,崔磊,刘珂,宗莹莹,袁虹霞,邢小萍,李洪杰,李洪连. 小麦品种太空6号对Heterodera avenae郑州群体的抗性遗传分析[J]. 作物学报, 2013, 39(04): 642-648.
[9] 王光，吴智丹，张磊，刘凤权，邵敏. 水稻稻瘟病菌诱导表达启动子OsQ16p的克隆与功能分析[J]. 作物学报, 2012, 38(06): 980-987.
[10] 崔磊，高秀，王晓鸣，简恒，唐文华，李洪连，李洪杰. 不同抗性小麦根与菲利普孢囊线虫(Heterodera filipjevi)互作的表型特征[J]. 作物学报, 2012, 38(06): 1009-1017.
[11] 高秀，崔磊，李洪连，王晓鸣，唐文华，Robert L. CONNER，林小虎，李洪杰. 硬粒小麦品种Waskana和Waskowa对禾谷孢囊线虫(Heterodera filipjevi和H. avenae)的抗性[J]. 作物学报, 2012, 38(04): 571-577.
[12] Moytri ROYCHOWDHURY, 贾育林,Richard D.CARTWRIGHT. 水稻稻瘟病抗病基因的结构功能和共同进化[J]. 作物学报, 2012, 38(03): 381-393.
[13] 袁虹霞, 张福霞, 张佳佳, 侯兴松, 李洪杰, 李洪连. CIMMYT小麦种质资源对菲利普孢囊线虫(Heterodera filipjevi)河南许昌群体的抗性[J]. 作物学报, 2011, 37(11): 1956-1966.
[14] 李洪杰, 王晓鸣, 宋凤景, 伍翠平, 武小菲, 张宁, 周阳, 张学勇. 中国小麦品种对白粉病的抗性反应与抗病基因检测[J]. 作物学报, 2011, 37(06): 943-954.
[15] 李余生, 黄胜东, 杨娟, 王才林. 水稻抗稻曲病数量性状座位及效应分析[J]. 作物学报, 2011, 37(05): 778-783.
Viewed
Full text
1070
HTML PDF
Just accepted Online first Issue Just accepted Online first Issue
0 0 0 0 0 1070

  From Others local
  Times 12 1058
  Rate 1% 99%

Abstract
408
Just accepted Online first Issue
0 0 408
  From Others local
  Times 50 358
  Rate 12% 88%

Cited
Web of Science  Crossref   ScienceDirect  Search for Citations in Google Scholar >>
 
This page requires you have already subscribed to WoS.
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2