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作物学报 ›› 2010, Vol. 36 ›› Issue (06): 1055-1060.doi: 10.3724/SP.J.1006.2010.01055

• 研究简报 • 上一篇    

磷肥对小麦籽粒HMW-GS积累及GMP粒度分布的影响

倪英丽1,王振林1,*,李文阳1,2,闫素辉1,2,尹燕枰1,李勇1,王平1,陈晓光1   

  1. 1作物生物学国家重点实验室/山东农业大学农学院，山东泰安271018；2安徽科技学院植物科学学院，安徽凤阳233100
  • 收稿日期:2009-12-08 修回日期:2010-03-19 出版日期:2010-06-12 网络出版日期:2010-04-14
  • 通讯作者: 王振林, E-mail: zlwang@sdau.edu.cn; zlwangsd@sina.com; Tel: 0538-8241359
  • 基金资助:

    本研究由国家自然科学基金项目（30871477），国家重点基础研究发展计划（973计划）项目（2009CB2118602）和国家公益性行业（农业）科研专项（200803037）资助。

Effects of Phosphorus Fertilizer on Accumulation of High Molecular Weight Glutenin Subunits and Glutenin Macropolymer Size Distribution in Wheat Grain

NI Ying-Li1,WANG Zhen-Lin1*,LI Wen-Yang12,YAN Su-Hui1,2,YIN Yan-Ping1,LI Yong1,WANG Ping1,CHEN Xiao-Guang1   

  1. 1National Key Laboratory of Crop Biology/Agronomy College,Shandong Agricultural University,Tai'an 271018,China;2College of Plant Science,Anhui Science and Technology University,Fengyang 233100,China
  • Received:2009-12-08 Revised:2010-03-19 Published:2010-06-12 Published online:2010-04-14
  • Contact: WANG Zhen-Lin,E-mail: zlwang@sdau.edu.cn; zlwangsd@sina.com; Tel: 0538-8241359

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2165

被引次数

17

摘要：

在112.5 kg hm-2和225 kg hm-2两种氮水平下，检测了施磷量对强筋小麦山农12籽粒高分子量谷蛋白亚基(HMW-GS)积累及谷蛋白大聚合体(GMP)粒度分布的调控效应。结果表明，小麦籽粒HMW-GS在花后14 d已形成, 成熟期籽粒HMW-GS含量表现为施磷处理高于不施磷处理。在低氮条件下，施磷有助于HMW-GS的积累，而在正常施氮条件下过多施磷则不利于其积累。在低氮条件下，粒径<10 μm GMP颗粒体积百分比随施磷量增加而显著降低；在正常氮水平下，施磷降低粒径<10 μm GMP颗粒体积百分比，其效应表现施磷0 kg hm-2处理最大，其次为施磷40 kg hm-2和100 kg hm-2处理，施磷160 kg hm-2处理最小。两种施氮水平下分别增施磷肥，粒径在10~100 μm和>100 μm GMP颗粒的体积百分比均呈现随磷肥用量增加而增加的趋势。在正常氮水平下，各施磷处理间籽粒中GMP颗粒数目百分比无明显差异。成熟期籽粒中HMW-GS含量与粒径<10 μm GMP颗粒体积百分比呈显著负相关，而与10~100 μm GMP颗粒体积百分比呈显著正相关。说明较大粒径GMP颗粒具有较高的HMW-GS含量。

关键词: 小麦, 高分子量谷蛋白亚基（HMW-GS）, 谷蛋白大聚合体（GMP）, 磷素, 氮素

Abstract:

For gaining insights into the relationship between the accumulation of high-molecular-weight glutenin subunit (HMW-GS) and the distribution of glutenin macropolymer (GMP) in wheat (Triticum aestivum L.), Shannong 12, a cultivar with high glutenin, was used in a field experiment with four phosphorus (P) application levels (0, 40, 100, and 160 kg ha-1) under low nitrogen (112.5 kg ha-1) and normal nitrogen (225 kg ha-1) conditions, respectively. The result showed that HMW-GS in grains formed before 14 d after anthesis, and its content at maturity was higher in P application treatments than in the treatment with no P fertilizer applied. Phosphorus was in favor of the accumulation of HMW-GS under the low nitrogen (N) condition, but caused a negative effect in high P levels under the normal N condition. The content of GMP particle in <10 μm in diameter was significantly reduced with increasing P application. The effect increased with increasing P level under the low N condition, but showed the largest reduction at P level of 100 kg ha-1 and the smallest reduction at P level of 40 kg ha-1. For GMP particle in 10–100 μm and >100 μm groups, their contents tended to increasing with more P applied under both N conditions. Under the normal N condition, there was no significant effect of P level on the number distribution of GMP particle. At maturity, the content of HMW-GS was negatively correlated with the volume of GMP particle in <10 μm group, but positively correlated with that in 10–100 µm group. The result suggested that larger GMP particles have higher HMW-GS content than the smaller GMP particles.

Key words: Wheat, High-molecular-Weight glutenin subunit(HMW-GS), Glutenin macropolymer(GMP), Phosphorus, Nitrogen

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