大豆幼苗根系性状的QTL分析

doi:10.3724/SP.J.1006.2011.01151

摘要/Abstract

摘要： 为研究大豆幼苗期根系性状的遗传规律，以中豆29和中豆32构建的RIL群体为材料，在V2期测定水培幼苗根系性状(主根长、侧根数、根重、根体积和根冠比等)及相关性状(株重、茎叶重和下胚轴重等)，以方差分析方法估算遗传参数，并采用复合区间作图法对大豆幼苗期根系等性状进行QTL定位。结果表明，在8个染色体上检测到20个根系及相关性状QTL，其中9个主效QTL位于第11和第14染色体，表型贡献率在10.5%~26.1%之间。在第11和第14染色体上，部分根系性状QTL与地上部性状QTL处于同一位置，其QTL的共位性与形态性状表型相关分析结果一致，反映了根系性状与地上部性状存在一定的关联。

关键词: 大豆, 根系性状, 幼苗期, QTL定位

Abstract: Root system plays important roles in plant growth and development, including uptaking water and nutrient, resisting biotic and abiotic stresses, and finally influencing yield and quality. A number of QTLs for shoot traits have been detected using different populations in soybean [Glycine max (L.) Merr], but rarely for root trait. The aim of the present study was to identify QTLs for some root traits at seedling stage in soybean. A total of 165 recombinant inbred lines (RILs) derived from the cross between Zhongdou 29 and Zhongdou 32 were used. Soybean seedlings were hydroponically cultured,root traits including main root length, number of lateral roots, root weight, root volume, and aerial part traits such as plant weight, shoot weight per plant, and ratio of root weight to shoot weight were measured at the stage of V2 (first trifoliolate leaf fully expanded). By composite interval mapping (CIM) method, twenty QTLs for roots and aerial part traits were mapped on eight chromosomes, including nine major-effect QTLs on chromosome 11 and 14 with 10.5–26.1% of the phenotypic variation. On chromosome 11 and 14, some QTLs for root traits and aerial part traits were clustered, which was consistent with the correlation analysis of phenotypic traits, indicating that the root traits are associated with the shoot traits.

Key words: Soybean, Root traits, Seedling stage, QTL mapping

周蓉, 陈海峰, 王贤智, 伍宝朵, 陈水莲, 张晓娟, 吴学军, 杨中路, 邱德珍, 江木兰, 周新安. 大豆幼苗根系性状的QTL分析[J]. 作物学报, 2011, 37(07): 1151-1158.

ZHOU Rong, CHEN Hai-Feng, WANG Xian-Zhi, WU Bao-Duo, CHEN Shui-Lian, ZHANG Xiao-Juan, TUN Hua-Jun, YANG Zhong-Lu, QIU De-Zhen, JIANG Mu-Lan, ZHOU Xin-An. QTL Analysis of Root Traits of Soybean at Seedling Stage[J]. Acta Agron Sin, 2011, 37(07): 1151-1158.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2011.01151

https://zwxb.chinacrops.org/CN/Y2011/V37/I07/1151

参考文献

[1]Keller M, Karutz Ch, Schmid J E, Stamp P, Winzeler M, Keller B, Messmer M M. Quantitative trait loci for lodging resistance in a segregating wheat×spelt population. Theor Appl Genet, 1999, 98: 1171–1182
[2]Menchey E K, Aycock Jr M K. Anther-derived dihaploids for lodging improvement in tobacco. Crop Sci, 1998, 38: 698–701
[3]Tar’an B, Warkentin T, Somers D J, Miranda D, Vandenberg A, Blade S, Woods S, Bing D, Xue A, DeKoeyer D, Penner G. Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theor Appl Genet, 2003, 107: 1482–1491
[4]Inoue M, Gao Z S, Cai H W. QTL analysis of lodging resistance and related traits in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet, 2004, 109:1576–1585
[5]Zhou R(周蓉), Wang X-Z(王贤智), Chen H-F(陈海峰), Zhang X-J(张晓娟), Shan Z-H(单志慧), Wu X-J(吴学军), Cai S-P(蔡淑平), Qiu D-Z(邱德珍), Zhou X-A(周新安), Wu J-S(吴江生). QTL analysis of lodging and related traits in soybean. Acta Agron Sin (作物学报), 2009, 35(1): 57–65 (in Chinese with English abstract)
[6]Liu Y(刘莹), Gai J-Y(盖钧镒), Lü H-N(吕慧能), Wang Y-J(王永军), Chen S-Y(陈受宜). Identification of drought tolerance germplasm and inheritance and QTL Mapping of related root traits in soybean
[Glycine max (L) Merr.]. Acta Genet Sin (遗传学报), 2005, 32(8): 855–863 (in Chinese with English abstract)
[7]Caradus J R. Genetic control of phosphorus uptake and phosphorus status in plants. In: Genetic Manipulation of Crop Plants to Enhance Integrated Nutrient Management in Cropping System. Patancheru, India: ICRISAT Asia Centre, 1995. pp 55–74
[8]Pantalone V R, Buton J W, Carter T E Jr. Soybean fibrous root heritability and genotypic correlations with agronomic and seed quality traits. Crop Sci, 1996, 36: 1120–1125
[9]Tian P-Z(田佩占). Ecotypes of root system in soybean cultivars. Acta Agron Sin (作物学报), 1984, 10(3): 173–178 (in Chinese with English abstract)
[10]Liu Y(刘莹), Gai J-Y(盖钧镒), Lü H-N(吕慧能). Identification of rhizosphere abiotic stress tolerance and related root traits in soybean
[Glycine max (L) Merr.]. Acta Agron Sin (作物学报), 2005, 31(9): 1132–1137 (in Chinese with English abstract)
[11]Hudak C M, Patterson R P. Vegetative growth analysis of a drought-resistant soybean plant introduction. Crop Sci, 1995, 35: 464–471
[12]Hudak C M, Patterson R P. Root distribution and soil moisture depletion pattern of a drought-resistant soybean plant introduction. Agron J, 1996, 88: 478–485
[13]Pantalone V R, Rebetzke G J, Burton J W, Carter T E Jr. Phenotypic evaluation of root traits in soybean and applicability to plant breeding. Crop Sci, 1996, 36: 456–459
[14]Sloane R J, Patterson R P, Carter T E Jr. Field drought tolerance of a soybean plant introduction. Crop Sci, 1990, 30: 118–123
[15]Hoogenboom G M, Huck C M, Peterson R P. Root growth rate of soybean as affected by drought stress. Agron J, 1987, 79: 607–614
[16]King C A, Purcell L C, Brye K R. Differential wilting among soybean genotypes in response to water deficit. Crop Sci, 2009, 49: 290–291
[17]Sinclair T R, Zwieniecki M A, Holbrook N M. Low leaf hydraulic conductance associated with drought tolerance in soybean. Physiol Plant, 2008, 132: 446–451
[18]Manavalan L P, Guttikonda S K, Tran L S P, Nguyen H T. Physiological and molecular approaches to improve drought resistance in soybean. Plant Cell Physiol, 2009, 50: 1260–1276
[19]Liang Q(梁泉), Yin Y-P(尹元萍), Yan X-L(严小龙), Liao H(廖红). Genetic analysis of root characters in soybean using a recombinant inbred line population at two phosphorus levels. Mol Plant Breed (分子植物育种), 2009, 7(2): 321–329 (in Chinese with English abstract)
[20]Zhang Y L, Jia J H, Zhao Y Q, Gu S Y, XU J G. Screening index for low phosphorus tolerance at seedling stage. Agric Sci Technol, 2010, 11 :87–89
[21]Wang H-L(王宏林), Yu D-Y(喻德跃), Wang Y-J(王永军), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). Mapping QTLs of soybean root weight with RIL population NJRIKY. Hereditas (遗传), 2004, 26(3): 333–336 (in Chinese with English abstract)
[22]Cui S-Y(崔世友), Geng L-Y(耿雷跃), Meng Q-C(孟庆长), Yu D-Y(喻德跃). QTL mapping of phosphorus deficiency tolerance in soybean (Glycine max L.) during seedling stage. Acta Agron Sin (作物学报), 2007, 33(3): 378–383 (in Chinese with English abstract)
[23]Liu Y(刘莹), Zhang M-C(张孟臣). Study on root trait related yield in soybean growing in summer and QTL mapping. J Hebei Univ Eng (Nat Sci Edn)(河北工程大学学报•自然科学版), 2010, 27(1): 65–69 (in Chinese with English abstract)
[24]Sun G-Y(孙广玉), He Y(何庸), Zhang R-H(张荣华), Zhang D-P(张代平). Studies on growth and activities of soybean root. Soybean Sci (大豆科学), 1996, 15(4): 317–321 (in Chinese with English abstract)
[25]Zhou R(周蓉), Chen H-F(陈海峰), Wang X-Z(王贤智), Zhang X-J(张晓娟), Shan Z-H(单志慧), Wu X-J(吴学军), Cai S-P(蔡淑平), Qiu D-Z(邱德珍), Zhou X-A(周新安), Wu J-S(吴江生). QTL analysis of yield, yield components, and lodging in soybean. Acta Agron Sin (作物学报), 2009, 35(5): 821–830 (in Chinese with English abstract)
[26]Zhou R(周蓉), Chen H-F(陈海峰), Wang X-Z(王贤智), Zhang X-J(张晓娟), Shan Z-H(单志慧), Wu X-J(吴学军), Qiu D-Z(邱德珍), Wu B-D(武宝朵), Sha A-H(沙爱华), Yang Z-L(杨中路), Zhou X-A(周新安). Dynamic analysis of QTL for plant height and stem diameter at different developmental stages in soybean. J Plant Genet Resour (植物遗传资源学报), 2010, 11(3): 349–359 (in Chinese with English abstract)
[27]Gai J-Y(盖钧镒). Experimentation Methods(试验统计方法), 3rd edn. Beijing: China Agriculture Press, 2000. pp 248–252 (in Chinese)
[28]Ma Y-H(马育华). Foundation of Statistical Genetics and Plant Breeding (植物育种的数量遗传学基础). Jiangsu: Jiangsu Sci & Tech Press, 1984. pp 442–445 (in Chinese)
[29]Wang S C, Basten C J, Zeng Z B. Windows QTL Cartographer 2.5 User Manual. Department of Statistics, North Carolina State University, Raleigh, NC, 2005 (http://statgen.ncsu.edu/qtlcart/ WQTLCart.htm)
[30]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report o QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13
[31]Ray J D, Yu L X, Mccouch S R, Mackill D J, Toole T C O, Huang N, MeCouch S R. Mapping quantitative trait loci associated with root penetration ability in rice (Oryza sativa L.). Theor Appl Genet, 1996, 92: 627–636
[32]Fang P(方萍), Wu P(吴平), Tao Q-N(陶勤南). QTLs for rice root morphological characters. Acta Agron Sin (作物学报), 1999, 25(2): 181–185 (in Chinese with English abstract)
[33]Li Z-K(李卓坤), Peng T(彭涛), Zhang W-D(张卫东), Xie Q-G(谢全刚), Tian J-C(田纪春). Analysis of QTLs for root trait at seedling stage using an “immortalized F2” population of wheat. Acta Agron Sin (作物学报), 2010, 36(3): 442–448 (in Chinese with English abstract)
[34]Lü C-X(吕彩霞), Guo J-Q(郭建秋), Wang Y(王英), Leng J-T(冷建田), Yang G-M(杨光明), Hou W-S(侯文胜), Wu C-X(吴存祥), Han T-F(韩天富). Identification, inheritance analysis, and QTL mapping of root and shoot traits in soybean variety PI471938 with tolerance to wilting. Acta Agron Sin (作物学报), 2010, 36(9): 1476–1483 (in Chinese with English abstract)
[35]Yang X-H(杨秀红), Wu Z-P(吴宗璞), Zhang G-D(张国栋). Study on soybean root system. J Northeast Agric Univ (东北农业大学学报), 2002, 33(2): 203–208 (in Chinese with English abstract)
[36]Lee S H, Park K Y, Lee H S, Park E H, Boerma H R. Genetic mapping of QTLs conditioning soybean sprout yield and Quality. Theor Appl Genet, 2001, 103: 702–709

相关文章 15

[1]	金昱何, 王雪菲, 徐张一娃, 缪怡宁, 蒋云杰, 伊莹, 缪德麟, 朱静仪, 钟一帆, 陈铭亨, 方芳, 刘鹏. 外源激素对低温胁迫下大豆叶片叶绿素荧光参数及抗氧化酶系统的影响[J]. 作物学报, 2026, 52(6): 1817-1829.
[2]	唐宽强, 李公允, 宋美毅, 赵雪, 常春玲. 大豆株高性状全基因组关联分析及预测模型构建[J]. 作物学报, 2026, 52(6): 1743-1756.
[3]	郑玉珍, 齐飞艳, 孙子淇, 刘华, 秦利, 石磊, 王娟, 汪蒙蒙, 韩锁义, 徐静, 苗利娟, 黄冰艳, 董文召, 郑峥, 张新友. 花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J]. 作物学报, 2026, 52(6): 1646-1657.
[4]	姚术, 郭凯悦, 翟慧慧, 姚佳慧, 邓文琪, 闫玲, 黄驰, 高阳, 俞嫣然, 赵振邦, 李英慧, 王晓波, 李佳佳. 大豆苗期耐低铁综合评价及优异种质筛选[J]. 作物学报, 2026, 52(5): 1373-1387.
[5]	刘长友, 王珅, 时会影, 沈颖超, 孙蕾, 王彦, 张志肖, 苏秋竹, 田静, 范保杰. 基于饭豆基因资源的小豆远缘杂交群体抗豆象QTL定位[J]. 作物学报, 2026, 52(3): 936-944.
[6]	张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493.
[7]	王克晶, 李向华. 我国珍稀的大豆属多年生烟豆和短绒野大豆物种遗传资源濒危性评估分析[J]. 作物学报, 2025, 51(8): 2009-2019.
[8]	孟然, 李赵嘉, 冯薇, 陈悦, 刘路平, 杨春燕, 鲁雪林, 王秀萍. 大豆不同生育时期耐盐性综合评价及耐盐种质筛选[J]. 作物学报, 2025, 51(8): 1991-2008.
[9]	贺红利, 张雨涵, 杨静, 程云清, 赵杨, 李星诺, 司洪亮, 张兴政, 杨向东. 大豆e1-as基因突变体的创制及生理分析[J]. 作物学报, 2025, 51(8): 2228-2239.
[10]	邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735.
[11]	胡蒙, 沙丹, 张晟瑞, 谷勇哲, 张世碧, 李静, 孙君明, 邱丽娟, 李斌. 大豆分枝数QTL定位及候选基因筛选[J]. 作物学报, 2025, 51(7): 1747-1756.
[12]	杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
[13]	王琼, 邹丹霞, 陈兴运, 张威, 张红梅, 刘晓庆, 贾倩茹, 魏利斌, 崔晓艳, 陈新, 王学军, 陈华涛. 大豆开花时间和成熟期性状全基因组关联分析与候选基因预测[J]. 作物学报, 2025, 51(6): 1558-1568.
[14]	殷丛丛, 李睿琦, 岳霈尧, 李晨, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 基于闭合哑铃介导等温扩增可视化检测大豆花叶病毒SC15方法的建立及应用[J]. 作物学报, 2025, 51(5): 1248-1260.
[15]	张金泽, 周庆国, 肖莉晶, 金海润, 欧阳青静, 龙旭, 晏中彬, 田恩堂. 芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析[J]. 作物学报, 2025, 51(5): 1166-1177.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed