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作物学报 ›› 2012, Vol. 38 ›› Issue (06): 962-970.doi: 10.3724/SP.J.1006.2012.00962

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用关联分析发掘小麦自然群体旗叶叶绿素含量的优异等位变异

李玮瑜1,2，张斌2，张嘉楠2,3，昌小平2，李润植1,*，景蕊莲2,*   

  1. 1山西农业大学, 山西太谷 030801; 2中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部作物种质资源利用重点开放实验室, 北京 100081; 3国家谷子改良中心 / 河北省农林科学院谷子研究所, 河北石家庄 050031
  • 收稿日期:2012-01-19 修回日期:2012-04-18 出版日期:2012-06-12 网络出版日期:2012-04-23
  • 通讯作者: 景蕊莲, E-mail: jingrl@caas.net.cn, Tel: 010-82105829; 李润植, E-mail: rli2001@hotmail.com
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA100501)和国际农业研究磋商组织(CGIAR)挑战计划项目(G7010.02.01)资助

Exploring Elite Alleles for Chlorophyll Content of Flag Leaf in Natural Population of Wheat by Association Analysis

LI Wei-Yu1,2,ZHANG Bin2,ZHANG Jia-Nan2,3,CHANG Xiao-Ping2, LI Run-Zhi1,*,JING Rui-Lian2,*   

  1. 1 Agronomy College, Shanxi Agricultural University, Taigu 030801, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 National Millet Improvement Center of China, Institute of Millet Crops / Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
  • Received:2012-01-19 Revised:2012-04-18 Published:2012-06-12 Published online:2012-04-23
  • Contact: 景蕊莲, E-mail: jingrl@caas.net.cn, Tel: 010-82105829; 李润植, E-mail: rli2001@hotmail.com

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被引次数

57

摘要： 为揭示小麦自然群体干旱胁迫条件下旗叶叶绿素含量的变化, 筛选相关标记的优异等位变异, 以262份小麦种质资源组成的自然群体为材料, 分别种植在北京的2个试验地点, 均设雨养和灌溉处理, 于开花期和灌浆期检测旗叶叶绿素含量。以分布于21条染色体的169个SSR标记检测所有材料的基因型, 利用STRUCTURE 2.3.2软件分析群体结构, 用TASSEL软件的MLM (mixed linear model)方法对小麦自然群体的旗叶叶绿素含量进行关联分析。在此基础上, 将携带某等位变异的所有材料表型均值与携带无效等位基因(null allele)材料表型均值比较, 估计等位变异的表型效应, 鉴别优异等位变异。共检测到2048个等位变异, 每位点2~37个等位变异, 平均12个。每位点的标记多态性信息量(PIC)为0.008~0.936, 平均0.628。在22个标记位点共检测出40个(次)与旗叶叶绿素含量极显著的关联, 其中11个标记位点有2次以上的关联, Xwmc419-1B和Xgwm501-2B分别有3次关联。在Xcfa2123-7A、Xgwm232- 1D和Xgwm429-2B位点分别检测到效应值大于4.0的等位变异。

关键词: 小麦, 叶绿素含量, SSR, 关联分析, 等位变异

Abstract: Elite alleles associated with chlorophyll content of flag leaf in a natural population of winter wheat (Triticum aestivum L.) consisting of 262 accessions were measured at flowering and grain-filling stages under both rainfed and well-watered conditions in two experimental locations in Beijing, China. A total of 169 SSR markers distributed on the 21 chromosomes of wheat were employed to detect the genetic diversity and genetic structure of the population. Association analysis between SSR loci and chlorophyll content trait was performed using TASSEL MLM (mixed linear model) program. The phenotypic allele effect was estimated through comparing the average phenotypic value over accessions with that of “null allele”. A total of 2048 alleles were identified on the 169 SSR loci, and each locus had 2–37 alleles with an average of 12. The polymorphism information contents (PICs) of the SSR loci ranged from 0.008 to 0.936 with an average of 0.628. Forty markers were found to be significantly associated with chlorophyll content (P<0.001) on 22 loci, of which 11 markers showed repeated associations, particularly, loci Xwmc419-1B and Xgwm501-2B had associations for three times. The alleles on loci Xcfa2123-7A, Xgwm232-1D, and Xgwm429-2B exhibited more than 4.0 of phenotypic effect value.

Key words: Wheat (Triticum aestivum L.), Chlorophyll content, SSR, Association analysis, Allelic variation

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