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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1640-1648.doi: 10.3724/SP.J.1006.2012.01640

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大麦染色体1H至7H外源基因渗入系的构建及其分析

赖勇1,2，冯静霞1,2，司二静1,2，李葆春3，孟亚雄1,2，马小乐2，杨轲1,2，尚勋武2，王化俊1,2,*   

  1. 1甘肃省作物遗传改良与种质创新重点实验室 / 甘肃省干旱生境作物学重点实验室，甘肃兰州730070；2甘肃农业大学农学院，甘肃兰州 730070；
    3甘肃农业大学生命科学技术学院，甘肃兰州730070
  • 收稿日期:2012-01-15 修回日期:2012-05-13 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 王化俊, E-mail: whuajun@yahoo.com
  • 基金资助:

    本研究由国家科技支撑计划项目(2007BAD52B08)，国家自然科学基金项目(30771331)和国家现代农业产业技术体系建设项目(CARS-05)资助。

Development and Analysis of Introgression Lines on Chromosomes 1H–7H in Barley

LAI Yong1,2,FENG Jing-Xia1,2,SI Er-Jing1,2,LI Bao-Chun3,MENG Ya-Xiong1,2,MA Xiao-Le2,YANG Ke1,2,SHANG Xu-Wu2,WANG Hua-Jun1,2,*   

  1. 1 Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement / Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Life Sciences and Technology of Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-01-15 Revised:2012-05-13 Published:2012-09-12 Published online:2012-07-03
  • Contact: 王化俊, E-mail: whuajun@yahoo.com

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摘要： 以96对SSR引物对BC3F2群体进行辅助筛选，构建了一组基本覆盖野生大麦ISR42-8染色体，并导入轮回亲本Scarlett的1H~7H全基因组的外源基因渗入系，为大麦QTL精细定位提供了良好的作图群体。这组渗入系一共66个，目标片段平均长度为27.6 cM，最长的系IL-52片段长度为100.5 cM，最短的系IL-50片段长度为1.5 cM。含单个渗入片段的有33个系。聚类分析结果表明，66个渗入系的遗传背景高度相似，遗传相似系数变幅为0.708~1.000，平均值为0.917。检测到一个位于4H染色体87.5 cM到110.0 cM区间的分蘖相关QTL，长度为22.5 cM，其中包含多态标记MGB396。

关键词: 大麦, 基因渗入系, 分子标记, QTL定位

Abstract: To provide a suitable population for quantitative trait locus (QTL) mapping and genetic research of favorable genes in barley (Hordeum valgare L.), we constructed a set of introgression lines (ILs, BC3F2) with the genetic background of recurrent parent Scarlett using 96 SSR markers that cover the whole genome of wild barley ISR42-8. This IL population contained 66 lines, and the target segments were determined with the 96 SSR markers distributed on chromosomes 1H to 7H of barley. The average length of target segments was 27.6 cM. The line IL-52 carried the longest introgressed segment (100.5 cM), and the line IL-50 carried the shortest introgressed segment (1.5 cM). There were 33 lines containing a single segment. The clustering analysis showed that the genetic backgrounds of these introgression lines were rather similar with each other. The genetic similarity coefficients ranged from 0.708 to 1.000, with the mean of 0.917. A QTL for tiller number was mapped in the interval of 87.5–110.0 cM on chromosome 4H, the size of which was 22.5 cM, and the polymorphism marker was MGB396.

Key words: Barley, Introgression line, Molecular mark, QTL mapping

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