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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1640-1648.doi: 10.3724/SP.J.1006.2012.01640

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大麦染色体1H至7H外源基因渗入系的构建及其分析

赖勇1,2，冯静霞1,2，司二静1,2，李葆春3，孟亚雄1,2，马小乐2，杨轲1,2，尚勋武2，王化俊1,2,*   

  1. 1甘肃省作物遗传改良与种质创新重点实验室 / 甘肃省干旱生境作物学重点实验室，甘肃兰州730070；2甘肃农业大学农学院，甘肃兰州 730070；
    3甘肃农业大学生命科学技术学院，甘肃兰州730070
  • 收稿日期:2012-01-15 修回日期:2012-05-13 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 王化俊, E-mail: whuajun@yahoo.com
  • 基金资助:

    本研究由国家科技支撑计划项目(2007BAD52B08)，国家自然科学基金项目(30771331)和国家现代农业产业技术体系建设项目(CARS-05)资助。

Development and Analysis of Introgression Lines on Chromosomes 1H–7H in Barley

LAI Yong1,2,FENG Jing-Xia1,2,SI Er-Jing1,2,LI Bao-Chun3,MENG Ya-Xiong1,2,MA Xiao-Le2,YANG Ke1,2,SHANG Xu-Wu2,WANG Hua-Jun1,2,*   

  1. 1 Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement / Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Life Sciences and Technology of Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-01-15 Revised:2012-05-13 Published:2012-09-12 Published online:2012-07-03
  • Contact: 王化俊, E-mail: whuajun@yahoo.com

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摘要： 以96对SSR引物对BC3F2群体进行辅助筛选，构建了一组基本覆盖野生大麦ISR42-8染色体，并导入轮回亲本Scarlett的1H~7H全基因组的外源基因渗入系，为大麦QTL精细定位提供了良好的作图群体。这组渗入系一共66个，目标片段平均长度为27.6 cM，最长的系IL-52片段长度为100.5 cM，最短的系IL-50片段长度为1.5 cM。含单个渗入片段的有33个系。聚类分析结果表明，66个渗入系的遗传背景高度相似，遗传相似系数变幅为0.708~1.000，平均值为0.917。检测到一个位于4H染色体87.5 cM到110.0 cM区间的分蘖相关QTL，长度为22.5 cM，其中包含多态标记MGB396。

关键词: 大麦, 基因渗入系, 分子标记, QTL定位

Abstract: To provide a suitable population for quantitative trait locus (QTL) mapping and genetic research of favorable genes in barley (Hordeum valgare L.), we constructed a set of introgression lines (ILs, BC3F2) with the genetic background of recurrent parent Scarlett using 96 SSR markers that cover the whole genome of wild barley ISR42-8. This IL population contained 66 lines, and the target segments were determined with the 96 SSR markers distributed on chromosomes 1H to 7H of barley. The average length of target segments was 27.6 cM. The line IL-52 carried the longest introgressed segment (100.5 cM), and the line IL-50 carried the shortest introgressed segment (1.5 cM). There were 33 lines containing a single segment. The clustering analysis showed that the genetic backgrounds of these introgression lines were rather similar with each other. The genetic similarity coefficients ranged from 0.708 to 1.000, with the mean of 0.917. A QTL for tiller number was mapped in the interval of 87.5–110.0 cM on chromosome 4H, the size of which was 22.5 cM, and the polymorphism marker was MGB396.

Key words: Barley, Introgression line, Molecular mark, QTL mapping

[1]Steven D Tanksley, Susan R. McCouch. Seed banks and molecular maps: unlocking genetic potential from the wild. Science, 1997, 277: 1063–1064

[2]Tanksley S D, Nelson J C. Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet, 1996, 92: 191–203

[3]Xiao J, Li J, Yuan L, Thanksley S D. Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific cross. Theor Appl Genet, 1996, 92: 230–244

[4]Moncada P, Martinez C P, Borrero J, Chatel M, Gauch Jr H, Guimaraes E, Tohme J, McCouch S R. Quantitative trait loci for yield and yield components in an Oryza sativa × Oryza rufipogon BC2F2 population evaluated in an upland environment. Theor Appl Genet, 2001, 102: 41–52

[5]Paterson A H, Deverna J W, Lanini B, Tanksley S D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato. Genetics, 1990, 124: 735–742

[6]Young N D, Tanksley S D. RFLP analysis of the size of chromosomal segments retained around Tm-2 locus of tomato during backcross breeding. Theor Appl Genet, 1989, 77: 353–359

[7]Yan J B, Tang J H, Meng Y J, Ma X Q, Teng W T, Subhash C, Li L, Li J S. Improving QTL mapping resolution based on genotypic sampling-a case using a RIL population. Acta Genet Sin, 2006, 33: 617–624

[8]Lin S Y, Sasaki T, Yano M. Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines. Theor Appl Genet, 1998, 96: 997–1003

[9]Tian F, Li D J, Fu Q, Zhu Z F, Fu Y C, Wang X K, Sun C Q. Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits. Theor Appl Genet, 2006, 112: 570–580

[10]Pestsova E G, Borner A, Roder M S. Development of a set of Triticum aestivum–Aegilops tauschii introgression lines. Hereditas, 2001, 135: 139–143

[11]Wang J, Xiang F N, Xia G M. Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line. Planta, 2005, 221: 277–286

[12]Liu S B, Zhou R H, Dong Y C, Li P, Jia J Z. Development, utilization of introgression lines using a synthetic wheat as donor. Theor Appl Genet, 2006, 112: 1360-1373

[13]Chetelat R T, Meglic V. Molecular mapping of chromosome segments introgressed from Solanum lycopersicoides into cultivated tomato (Lycopersicon esculentum). Theor Appl Genet, 2000, 100: 232–241

[14]Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics, 1995, 141: 1147–1162

[15]Bernacchi D, Beck-Bunn D, Emmatty D, Eshed Y, Inai S, Lopez J, Petiard V, Sayama H, Uhlig J, Zamir D. Advanced backcross QTL analysis of tomato: II. Evaluation of near isogenic lines carrying single-donor introgressions for desirable wild QTL-alleles derived from Lycopersicon hirsutum and L. pimpinellifolium. Theor Appl Genet, 1998, 97: 170–180

[16]Pillen K, Zacharias A, Léon J. Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor Appl Genet, 2003, 107: 340–352

[17]Pillen K, Zacharias A, Léon J. Comparative AB-QTL analysis in barley using a single exotic donor of Hordeum vulgare ssp. spontaneum. Theor Appl Genet, 2004, 108: 1591–1601

[18]Matus I, Corey A, Filichkin T, Hayes P M, Vales M I, Kling J, Riera-Lizarazu O, Sato K, Powell W, Waugh R. Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. Genome, 2003, 46: 1010–1023

[19]Korff M V, Wang H, Léon J, Pillen K. Development of candidate introgression lines using an exotic barley accession (Hordeum vulgare ssp. spontaneum) as donor. Theor Appl Genet, 2004, 109: 1736–1745

[20]Schmalenbach I, Körber N, Pillen K. Selecting a set of wild barley introgression lines and verification of QTL effects for resistance to powdery mildew and leaf rust. Theor Appl Genet, 2008, 117: 1093–1106

[21]Andrew H P, Curt L B, Jonathan F W. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Rep, 1993, 11: 122–127

[22]Kicherer S, Backes G, Walther U, Jahoor A. Localising QTLs for leaf rust resistance and agronomic traits in barley (Hordeum vulgare L.). Theor Appl Genet, 2000, l00: 881–888

[23]Marquez-Cedillo L A, Hayes P M, Kleinhofs A, Legge W G, Rossnagel B G, Sato K, Ullrich S E, Wesenberg D M. QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups. Theor Appl Genet, 2001, 103: 625–637

[24]Teulat B, Borries C, This D. New QTLs identified for plant water status, water-soluble carbohydrate and osmotic adjustment in a barley population grown in a growth-chamber under two water regimes. Theor Appl Genet, 2001, 103: 161–170

[25]Korff M V, Wang H, Léon J, Pillen K. AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum). Theor Appl Genet, 2006, 112: 1221–1231

[26]Korff M V, Wang H, Léon J, Pillen K. AB-QTL analysis in spring barley: I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley. Theor Appl Genet, 2005, 112: 1221–1231
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