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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1054-1059.doi: 10.3724/SP.J.1006.2013.01054

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

新型水稻57H突变体的发掘与表征

车倩倩1,郭艳萍1,李强1,王益华2,王广元3,田怀东1,*   

  1. 1 山西大学生命科学学院，山西太原030006；2 南京农业大学作物遗传与种质创新国家重点实验室，江苏南京210095；3 山西省作物科学研究所，山西太原030031
  • 收稿日期:2012-12-04 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 基金资助:

    本研究由国家自然科学基金项目(31040058)资助。

Excavation and Characterization of Novel Rice 57H Mutants

CHE Qian-Qian1,GUO Yan-Ping1,LI Qiang1,WANG Yi-Hua2,WANG Guang-Yuan3,TIAN Huai-Dong1,*   

  1. 1 College of Life Science, Shanxi University, Taiyuan 030006, China; 2 State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 3 Shanxi Institute of Crop Science, Taiyuan 030031, China
  • Received:2012-12-04 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-22

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摘要：

在正常型水稻的发育种子中，谷蛋白与醇溶蛋白在胚乳细胞内质网被合成后，作为主要储藏蛋白质分别被蓄积成液泡型蛋白体与内质网衍生型蛋白体。一些57H变异导致57 kD谷蛋白前体高量增加与成熟型谷蛋白显著减少，是关于水稻储藏蛋白蓄积的基因突变。本研究采用SDS-PAGE技术，从水稻种质资源中，发现并获得2个57H突变体。种子全蛋白的SDS-PAGE分析显示, 这2个突变体兼具57 kD多肽链大量增加与13 kD多肽链缺失的性状，但并没有成熟型谷蛋白等其它储藏蛋白性状的变化；谷蛋白电泳与免疫印染分析显示它们中高量增加的多肽链是57 kD谷蛋白前体；醇溶蛋白电泳分析显示它们中缺失的多肽链是13 kD醇溶蛋白。这些结果表明所得的2份突变体具有与已报道57H突变体不同的性状。它们可能含有关于储藏蛋白质合成表达的未知遗传信息，是全面阐明储藏蛋白合成蓄积遗传调控机制的有用素材。

关键词: 水稻, 胚乳, 储藏蛋白质, 新型57H突变体

Abstract:

Glutelins and prolamins, the main rice storage proteins, are synthesized in the ER and accumulated into the vacuolar protein body and the ER-derived protein body in endosperm cell of the developing seed of the wild type rice, respectively. Several 57H mutations have been found to cause the high increase of 57 kD glutelin precursor and the remarkable decrease of the mature glutelins, and involved in the accumulation of storage proteins in rice. In this study, two 57H mutants were newly found and obtained from rice germplasms using SDS-PAGE technique. SDS-PAGE analysis of the total seed proteins showed that the two mutants had the characters namely the great increase of 57 kD polypeptide and the lack of 13 kD polypeptide, but not alteration of characters for the mature glutelins and other storage proteins; Electrophoretic and immuno-blotting analyses of glutelin showed that the increased polypeptides was 57 kD glutelin precursor in the mutants; Electrophoretic analysis of prolamins showed that the lacked polypeptide was 13kD prolamin in them. It is indicate that the two mutants have novel characters distinct from the reported 57H mutants. They probably contain unknown genetic information for synthesis and expression of storage proteins, and are considered to be useful materials for the overall understanding of genetic mechanism regulating synthesis and accumulation of the storage proteins in rice.

Key words: Rice, Endosperm, Storage proteins, Novel 57H mutants

 


 


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