欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (11): 2039-2045.doi: 10.3724/SP.J.1006.2013.02039

• 耕作栽培·生理生化 • 上一篇    下一篇

水稻细胞悬浮系的中短期保存方法

高义平1,2,吕孟雨1,赵和1,杨学举2,*,王海波1,*   

  1. 1河北省农林科学院遗传生理研究所,河北省植物转基因中心,河北石家庄050051;2河北农业大学农学院,河北保定071000
  • 收稿日期:2013-05-13 修回日期:2013-06-09 出版日期:2013-11-12 网络出版日期:2013-08-14
  • 通讯作者: 王海波, E-mail: nkywanghb@yahoo.com.cn; 杨学举, E-mail: shmyxj@hebau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(30270855)资助。

Short-medium Term Preservation of Rice Suspension Cells

GAO Yi-Ping1,2,LÜ Meng-Yu1,ZHAO He1,YANG Xue-Ju2,*,WANG Hai-Bo1,*   

  1. 1 Institute of Genetics and Physiology, Hebei Academy of Agricultural and Forestry Sciences, Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China; 2 College of Agriculture, Agricultural University of Hebei, Baoding 071001, China
  • Received:2013-05-13 Revised:2013-06-09 Published:2013-11-12 Published online:2013-08-14
  • Contact: 王海波, E-mail: nkywanghb@yahoo.com.cn; 杨学举, E-mail: shmyxj@hebau.edu.cn

摘要:

细胞悬浮系的中短期保存广泛需要。本文应用中花15细胞悬浮系,比较了AAN6MS三种固体培养基对水稻细胞悬浮系的繁殖保存效果,结果表明,AA固体培养基保存效果最好。细胞系可以在AA0培养基上4个月、在AA0.5培养基上6个月(中间45 d左右继代一次)连续保存后仍然保持其可重悬性。通过比较AA固体培养基繁殖保存、冷冻保存、连续悬浮培养3种保存方法,表明AA固体培养基繁殖保存细胞系2~9个月内,既可保持细胞的可悬浮性,又对细胞系的PODSOD活性和植株再生率影响较小,是一种理想的细胞悬浮系中短期保存方法。

关键词: 水稻, 悬浮细胞, 冷冻保存, 植株再生, POD活性, SOD活性

Abstract:

The preservation of cell suspension in short-medium term is widely needed in cell biology. In this paper, the preservation efects of cell suspension of rice cultivar Zhonghua 15 on AA, N6, and MS solid culture media were compared. The results showed that AA medium was suitable for suspension cells preservation. The cell suspension still kept resuspensible after growing continuously on AA solid medium for 4 months, and then on AA medium supplemented with 0.5 mg L-1 2,4-D for 6 months (subcultured at about 45 d interval). Three kinds of methods of suspension cells preservation, namely preservation on AA solid medium, cryopreservation, and continuously suspending culture, were compared. The results indicated that the first method kept good suspensible property, had high regeneration rate, and had a little influence on activity of POD and SOD, even though the suspension cells grew on AA medium for 2–9 months. AA solid medium is ideal for the preservation of suspension cells in short-middle-term.

Key words: Rice, Suspension cell, Cryopreservation, Plant regeneration, POD, SOD

[1]Wang M(王满), Li X(李霞), Shi M-D(石牡丹), Qian B-Y(钱宝云), Wei X-D(魏晓东), Fang X-W(方先文). Optiming cell suspension culture of mature embryo of transgenic C4 phosphoenolpyruvate carboxylase (pepc) rice. Mol Plant Breed(分子植物育种), 2012, 10 (6): 644−654 (in Chinese with English abstract)



[2]Wang C-Y(王藏月), Wang F-R(王凤茹), Dong J-G(董金皋). Proteomic analysis of rice suspension cultured cells treated with brassinosteroids. J Agric Univ Hebei (河北农业大学学报), 2011, 34(1): 62−67 (in Chinese with English abstract)



[3]Xu L-L(徐林林), Lu D(芦笛), Lu W(陆巍), Zhang R-X(张荣铣), Yang Q(杨清). Establishment of suspension cell line of rice (Oryza sativa L.) and effects of different media on biomass. Plant Physiol Commun (植物生理学通讯). 2006, 42(4): 612−616 (in Chinese with English abstract)



[4]Meijer E G M, Van I E, Schrijnemakers E, Hensgens L A M, Van Zijderveld M, Schilperoort R A. Retention of the capacity to produce plants from protoplasts in cryopreserved cell lines of rice (Oryza sativa L.). Plant Cell Rep, 1991, 10: 171−174



[5]Yin D-D(尹德东), Hu B-Z(胡宝忠). Establishment and cryopreservation of rice suspension cells line. J Northeast Agric Univ (东北农业大学学报), 2006, 37 (6): 750−754 (in Chinese with English abstract)



[6]Yan Q-F(严庆丰), Wang J-H(王君晖), Huang C-N(黄纯农), Yan Q-S(颜秋生), Zhang X-Q(张雪琴). Studies on cryopreservation of rice (Oryza sativa L.) suspension cultures. Acta Biol Exp Sin (实验生物学报), 1994, 27(4): 399−409 (in Chinese with English abstract)



[7]Cho J S, Hong S M, Joo S Y, Yoo J S, Kim D I. Cryopreservation of transgenic rice suspension cells producing recombinant hCTLA4Ig. Appl Microbiol Biotechnol, 2007, 73: 1470−1476



[8]Huang C-N(黄纯农), Wang J-H(王君晖), Yan Q-F(严庆丰), Yan Q-S(颜秋生), Zhang X-Q(张雪琴). Preservation of barley and rice cell suspension cultures in liguid nitrogen by vitrification. J Huangzhou Univ (Nat Sci)(杭州大学学报?自然科学版), 1994, 21 (1): 114−115 (in Chinese)



[9]Liu F(刘峰), Wang J-H(王君晖), Huang C-N(黄纯农), Yan Q-S(颜秋生), Zhang X-Q(张雪琴). Ultrastructural changes in rice embryogenic suspension cells cryopreserved by vitrification. Chin J Rice Sci(中国水稻科学), 1998, 12 (1): 17−20 (in Chinese with English abstract)



[10]Shibli R A, Haagenson D M, Cunningham S M, Berg W K, Volenec J J. Cryopreservation of alfalfa (Medicago sativa L.) cells by encapsulation-dehydration. Plant Cell Rep, 2001, 20: 445−450



[11]Zeng B-Y(曾博雅), Wang Z(王智), Zhang Y-F(张云峰), Yang Q(杨清), Lu W(陆巍). Cryopreservation of rice (Oryza sativa L.) embryonic cell suspensions by encapsulation-dehydration. Plant Physiol Commun (植物生理学通讯), 2009, 45(6): 603−606 (in Chinese with English abstract)



[12]Burritt D J. Efficient cryopreservation of adventitious shoots of Begonia ? erythrophylla using encapsulation-dehydration requires pretreatment with both ABA and praline. Plant Cell Tissue Organ Cult, 2008, 95: 209−215



[13]Hirai D, Sakai A. Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification. Potato Res, 1999, 42: 153−160



[14]Kong L S, von Aderkas P. A novel method of cryopreservation without a cryoprotectant for immature somatic embryos of conifer. Plant Cell Tiss Organ Cult, 2011, 106: 115−125



[15]Engelmann F. In vitro conservation methods. In: Callow J A, Ford-Lloyd B V, Newbhrg H J, eds. Biotechnology and Plant Genetic Resources. Oxford: CAB International, 1997. pp 119−161



[16]Wang Q C, Perl A. Cryopreservation in floricultural plants. In: Teixeira D A, Silva J A, eds. Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues. London: Global Science Books, 2006. pp 523−539



[17]Harding K. Genetic integrity of cryopreserved plant cells: a review. Cryo Lett, 2004, 25: 3−22



[18]Withers L A, Engelmann F. In vitro conservation of plant genetic resources. In: Altman A ed. Agricultural Biotechnology. New York, Marcel Dekker Inc., 1998. pp 57−88



[19]Benson E E. Cryopreservation of phytodiversity: a critical appraisal of theory practice. Crit Rev Plant Sci, 2008, 27(3): 141−219



[20]Panis B, Lambardi M. Status of cryopreservation technologies in plants (crops and forest trees). In: Ruane J, Sonnino A, eds. The Role of Biotechnology in Exploring and Protecting Agricultural Genetic Resources. Rome: FAO, 2006. pp 61−78



[21]Wang B, Yin Z F, Feng C H, Shi X, Li Y P, Wang Q C. Cryopreservation of potato shoot tips. In: Benkeblia N, Tennant P, eds. Potato I. Fruit, Vegetable and Cereal Science and Biotechnology 2 (Special Issue 1). London: Global Science Book, 2008. pp 46−53



[22]Feng C H, Yin Z F, Ma Y L, Zhang Z B, Chen L, Wang B, Li B Q, Huang Y S, Wang Q C. Cryopreservation of sweetpotato (Ipomoea batatas) and its pathogen eradication by cryotherapy. Biotechnol Adv, 2011, 29: 84−93



[23]Moriguchi T, Kozaki I, Yamaki S, Sanada T. Low temperature storage of pear shoots in vitro. Bull Fruit Tree Res Stn, 1990, 17: 11−18



[24]Wang H-B(王海波). Concept of the cell state and its significance in life science. Sci & Technol Rev (科技导报), 2008, 26 (4): 41−46 (in Chinese with English abstract)



[25]Xing D-H(邢登辉), Wu Q-S(吴琴生), Liu D-J(刘大钧). The cell culture of cereal crops- induction and expression of somatic embryogenic potential. Bull Biol (生物学通报), 1994, 29(7): l−3 (in Chinese)



[26]Li C-Y(李春燕), Chen S-S(陈思思), Xu W(徐雯), Li D-S(李东升), Gu X(顾骁), Zhu X-K(朱新开), Guo W-S(郭文善), Feng C-N(封超年). Effect of low temperature at seedling stage on antioxidation enzymes and cytoplasmic osmoticum of leaves in wheat cultivar Yangmai 16. Acta Agron Sin (作物学报), 2011, 37(12): 2293−2298 (in Chinese with English abstract)



[27]Coppens L, Gillis E. Isoenzyme electrofocusing as a biochemical marker system of embryogenesis and organogenesis in callus tissues of Hordeumvulgare. Plant Physiol, 1987, 127: 153−158



[28]Rewal S K, Mehta A R. Changes in enzyme activity and isoperoxidases in haploid tobacco callus during organogenesis. Plant Sci Lett, 1982, 24: 67−77

[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[6] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[7] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[8] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[9] 王小雷, 李炜星, 欧阳林娟, 徐杰, 陈小荣, 边建民, 胡丽芳, 彭小松, 贺晓鹏, 傅军如, 周大虎, 贺浩华, 孙晓棠, 朱昌兰. 基于染色体片段置换系群体检测水稻株型性状QTL[J]. 作物学报, 2022, 48(5): 1141-1151.
[10] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[11] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[12] 王吕, 崔月贞, 吴玉红, 郝兴顺, 张春辉, 王俊义, 刘怡欣, 李小刚, 秦宇航. 绿肥稻秆协同还田下氮肥减量的增产和培肥短期效应[J]. 作物学报, 2022, 48(4): 952-961.
[13] 巫燕飞, 胡琴, 周棋, 杜雪竹, 盛锋. 水稻延伸因子复合体家族基因鉴定及非生物胁迫诱导表达模式分析[J]. 作物学报, 2022, 48(3): 644-655.
[14] 陈云, 李思宇, 朱安, 刘昆, 张亚军, 张耗, 顾骏飞, 张伟杨, 刘立军, 杨建昌. 播种量和穗肥施氮量对优质食味直播水稻产量和品质的影响[J]. 作物学报, 2022, 48(3): 656-666.
[15] 王琰, 陈志雄, 姜大刚, 张灿奎, 查满荣. 增强叶片氮素输出对水稻分蘖和碳代谢的影响[J]. 作物学报, 2022, 48(3): 739-746.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!