作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1371-1379.doi: 10.3724/SP.J.1006.2014.01371
张林成1,高启国1,*,蒲全明1,任雪松1,刘豫东1,朱利泉2,王小佳1
ZHANG Lin-Cheng1,GAO Qi-Guo1,*,PU Quan-Ming1,REN Xue-Song1,LIU Yu-Dong1,ZHU Li-Quan2,WANG Xiao-Jia1,*
摘要:
bHLH类转录因子在植物叶片形态建成和发育过程中发挥重要的生理功能。本文通过结球甘蓝转录组数据分析,筛选出莲座期和结球期的茎尖间与叶片间均显著差异表达的2个bHLH类转录因子,命名为BoLH01和BoLH02基因,获得了2个基因的编码序列,其中BoLH01基因CDS全长为966 bp、编码321个氨基酸;BoLH02基因CDS全长870 bp、编码289个氨基酸;序列分析表明BoLH01和BoLH02分别与拟南芥AtbHLH18和AtbHLH19氨基酸同源相似性最高,达81%和74%,均具典型bHLH结构域特点和完全保守的13E-16R和9H-13E-17R以及Leu23对应的关键氨基酸位点;分子进化上BoLH01、BoLH02与AtTCP2/3/4/10/24亲缘关系较近,与AtTCP9/11/14/15较远;转录组和荧光定量PCR分析表明BoLH01和BoLH02在平展的莲座叶中表达量较低,在卷曲的球叶中高量表达;序列分析BoHB7、BoHB12和BoILL6的ATG上游均包含能被bHLH功能域识别的E-box序列,对BoHB7、BoHB12和BoILL6荧光定量PCR分析表明, 三者与BoLH01和BoLH02在不同时期叶片中表达量的变化趋势完全一致;说明BoLH01和BoLH02可能通过正向调控BoHB7、BoHB12、BoILL6基因的表达来参与甘蓝叶片卷曲的调控。
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