作物学报 ›› 2015, Vol. 41 ›› Issue (04): 531-538.doi: 10.3724/SP.J.1006.2015.00531
沈荔花1,2,李碧凉1,任勇杰1,李程勋1,钟永嘉2,方长旬1,2,林文雄1,2,*
SHEN Li-Hua1,2,LI Bi-Liang1,REN Yong-Jie1,LI Cheng-Xun1,ZHONG Yong-Jia2,FANG Chang-Xun1,2,LIN Wen-Xiong1,2,*
摘要:
基因表达调控是水稻化感作用形成的重要基础。本研究对化感水稻PI312777 (Oryza sativa L.)的OsMYB (CT829537)基因分别进行过表达(overexpression, OE)和RNA干扰(RNAi),再与稗草(Echinochloa crusgalli, BYG)共培养,以野生型PI312777为对照。结果发现,与稗草共培养下CT829537-OEPI312777的酚类代谢关键酶基因表达上调,根系及其水培液中的总酚酸浓度增加,抑草能力增强;相同处理下CT829537-RNAiPI312777则相反,其酚类代谢关键酶基因较野生型植株表达下调,总酚酸浓度降低,抑草能力下降。这表明OsMYB (CT829537)通过调节化感水稻的酚酸类物质合成进而影响其抑草能力。
| [1]Olofsdotter M, Navarez D, Rebulanan M, Streibig J C. Weed-suppressing rice cultivars: does allelopathy play a role. Weed Res, 1999, 39: 441–454[2]Chou C H, Chang F J, Oka H I. Allelopathic potentials of wild rice, Orzyaperennis. Taiwania, 1991, 36: 201–210[3]Chou C H. Adaptive autointoxication mechanisms of Oryza sativa. In: Olofsdotter M, ed. Allelopathy in Rice. Los Banzos, Philippines: IRRI, 1996. pp 25–27[4]Fujii Y. The allelopathic effect of some rice varities. In: Proceedings of the International Seminar Biological Control and Integrated Management of Paddy and Aquatic Weeds in Asia. Tsukuba, Japan, 1992. pp 160–165[5]Dilday R H, Lin J, Yan W. Identification of allelopathy in the USDA-ARS rice germplasm collection. Aust J Exp Agric, 1994, 34: 907–910[6]Dilday R H, Yan W G, Moldenhauer K A K, Gravois K A. Allelopathic activity in rice for controlling major aquatic weeds. In: Olofsdotter M, ed. Proceedings of Workshop on Allelopathy in Rice. Manila, Philippines: IRRI, 1998. pp 7–26[7]Dilday R H, Nastasi P, Smith R J Jr. Allelopathic observation in rice (Oryza sativa L.) to ducksalad (Heterantheralimosa). Proc Arkansas Acad Sci, 1989, 43: 21–22[8]Dilday R H, Lin J, Yan W. Identification of allelopathy in the USDA-ARS rice germplasm collection. Aust J Exp Agric, 1994, 34: 907–910[9]Dilday R H, Mattice J D, Moldenhauer K A. An overview of rice allelopathy in the USA. In: Kim K U, Shin D H, eds. Rice Allelopathy. Taegu (Korea): Kyungpook National University, 2000. pp 15–26[10]Jensen L B, Courtois B, Shin L S, Li Z K, Olofsdotter M, Mauleon R P. Locating genes controlling allelopathic effects against barnyardgrass in upland rice. Agron J, 2001, 93: 21–26[11]Ebana K, Yan W G, Dilday R, Namai H, Okuno K. Analysis of QTL associated with the allelopathic effect of rice using water soluble extracts. Breed Sci, 2001, 51: 47–51[12]Xiong J, Jia X L, Deng J Y. Analysis of epistatic effect and QTL interactions with environment for allelopathy in rice (Oryza sativa L.). Allelopathy J, 2007, 20: 259–268[13]Lee S B, Seo K I, Koo J H, Hur H S, Shin J C. QTLs and molecular markers associated with rice allelopathy. In: Haper J D I, An M, Kent J H, eds. Proceedings of the Fourth World Congresson Allelopathy “Establishing the scientific base”. Australia: Charles Sturt Universit, Wagga Wagga, NSW, 2005. pp 505–507[14]Zeng D L, Qian Q, Teng S, Fujimoto H, Kunihifo Y, Zhu L H. Genetic analysis of rice allelopathy. Chin Sci Bull, 2003, 48: 265–268[15]王海斌, 何海斌, 熊君, 邱龙, 方长旬,曾聪明, 严琳, 林文雄. 低钾胁迫对水稻(Oryza sativa L.)化感潜力变化的影响. 生态学报, 2008, 28: 6219–6227Wang H B, He H B, Xiong J, Qiu L, Fang C X, Zeng C M, Yan L, Lin W X. Effects of potassium stress on allelopathic of rice (Oryza sativa L.). Acta Ecol Sin, 2008, 28: 6219–6227 (in Chinese with English abstract)[16]王海斌, 何海斌, 叶陈英, 邱龙, 方长旬, 林文雄. 不同化感潜力水稻秧苗响应低钾的光合生理特性. 中国生态农业学报, 2008, 16: 1474–1477Wang H B, He H B, Ye C Y, Qiu L, Fang C X, Lin W X. Photosynthetic physiology of different allelopathic rice accessions at seedling stage under phtossium stress. Chin J Eco-Agric, 2008, 16: 1474–1477 (in Chinese with English abstract)[17]王海斌, 何海斌, 曾聪明, 吴良展, 沈荔花, 熊君, 林瑞余, 林文雄. 低磷胁迫下不同化感潜力水稻秧苗生长的分子生理特性. 应用与环境生物学报, 2008, 14: 593–598Wang H B, He H B, Zeng C M, Wu L Z, Shen L H, Xiong J, Lin R Y, Lin W X. Molecular physiological properties of different rice accessions mediated by different phosphorus supplies at seedling stage. Chin J Appl Environ Biol, 2008, 14: 593–598 (in Chinese with English abstract)[18]Mattice J, Lavy T, Sdulman B, Dilday R H. Searching for allelochemicals in rice that control ducksalad. In: Olofsdotter M ed. Proceedings of Workshop on Allelopathy in Rice. Manila, Philippines: IRRI, 1998. pp81–98[19]Seal A N, Pratley J E, Haig T, An M. Identification and quantitation of compounds in a series of allelopathic and nonallelopathic rice root exudates. J Chem Ecol, 2004, 30: 1647–1662[20]Macias F A, Chinchilla N, Varela R M, Molinllo J M G. Bioactive steroids from Oryza sativa L. Steroids, 2006, 71: 603–608[21]He H B, Wang H B, Fang, C X, Lin Z H, Yu Z M, Lin W X. Separation of allelopathy from resource competition using rice/barnyardgrass mixed-cultures. PLoS One, 2012, 7(5): e37201[22]Kato N H. Barnyard grass-induced rice allelopathy and momilactone B. Plant Physiol, 2011, 168: 1016–1020[23]Fang C X, He H B, Wang Q S, Qiu L, Wang H B, Zhuang Y E, Xiong J, Lin W X. Genomic analysis of allelopathic response to low nitrogen and barnyardgrass competition in rice (Oryza sativa L.). Plant Growth Regul, 2010, 61: 277–286[24]Fang C X, Zhuang Y E, Xu T C, Li Y Z, Li Y, Lin W X. Changes in rice allelopathy and rhizosphere microflora by inhibiting rice henylalanine ammonia-lyase gene expression. J Chem Ecol, 2013, 39: 204–212[25]Putu S, Tsutomu S, Hidenari S, Masahiro N, Masayuki N, Minco K. Development of simple and efficient in planta transformation method for rice (Oryza sativa L.) using Agrobacterium tumefaciens. J Biosci Bioeng, 2005, 100: 391–397[26]Fang C X, Wang Q S, Yu Y, Lin Q M, Zhang H L, Wu X C, Chen T, Lin W X. Suppression and overexpression of Lsi1 induce differential gene expression in rice under ultraviolet radiation. Plant Growth Regul, 2011, 65: 1–10[27]熊君, 王海斌, 方长旬, 邱龙, 吴文祥, 何海斌, 林文雄. 不同氮素供应下水稻酚类物质代谢关键酶基因差异表达. 植物生理与分子生物学学报, 2007, 33: 387–394Xiong J, Wang H B, Fang C X, Qiu L, Wu W X, He H B, Lin W X. The differential expression of the genes of the key enzymes involved in phenolic compound metabolism in rice (Oryza sativa L.) under different nitrogen supply. J Plant Physiol Mol Biol, 2007, 33: 387–394 (in Chinese with English abstract)[28]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 2001, 25: 402–408[29]庄月娥. 水稻抑草作用关键基因的分子生态机制研究. 福建农林大学硕士学位论文, 福建福州, 2011Zhuang Y E. Molecular Ecological Mechanism of the Key Gene for Weed-suppression in Allelopathic Rice. MS Thesis of Fujian Agriculture and Forestry University, Fuzhou, China, 2011 (in Chinese with English abstract)[30]Song B Q, Xiong J, Fang C X, Qiu L, Lin R Y, Liang Y Y, Lin W X. Allelopathic enhancement and differential gene expression in rice under low nitrogen treatment. J Chem Ecol, 2008, 34: 688–695[31]Xiong J, Wang H, Qiu L, Wu H W, Chen R S, He H B, Lin R Y, Lin W X. qRT-PCR analysis of key enzymatic genes related to phenolic acid metabolism in rice accessions (Oryza sativa L.) exposed to low nitrogen treatment. Allelopathy J, 2010, 25: 345–356[32]王海斌, 熊君, 方长旬, 邱龙, 吴文祥, 何海斌, 林文雄. 氮素胁迫下强、弱化感水稻萜类代谢途径中关键酶基因差异表达的FQ-PCR分析. 作物学报, 2007, 33(8): 1329–1334Wang H B, Xiong J, Fang C X, Qiu L, Wu W X, He H B, Lin W X. FQ-PCR analysis on the differential expression of the key enzyme genes involved in isoprenoid metabolic pathway in allelopathic and weak allelopathic rice accessions (Oryza sativa L.) under nitrogen stress condition. Acta Agron Sin(作物学报), 2007, 33(8): 1329–1334(in Chinese with English abstract)[33]孙小霞, 王海斌, 林辉锋, 何海斌, 陆锦池, 曾聪明, 熊君, 林文雄. 田间旱育条件下不同化感潜力水稻的抑草效应分析. 中国生态农业学报, 2009, 17: 842–846Sun X X, Wang H B, Lin H F, He H B, Lu J C, Zeng C M, Xiong J, Lin W X. Effects of weed suppression by different allelopathic rice varieties under dry-raising condition. Chin J Eco-Agric, 2009, 17: 842–846 (in Chinese with English abstract)[34]孙小霞, 王海斌, 何海斌, 陆锦池, 林文雄. 田间旱育条件下不同化感潜力水稻根际土壤酚酸类和萜类物质分析. 中国生态农业学报, 2014, 22: 806–812Sun X X, Wang H B, He H B, Lu J C, Lin W X. Analysis of phenolic acids and terpenoids in rhizosphere soils of different allelopathic rice varieties under dry field conditions. Chin J Eco-Agric, 2014, 22: 806–812 (in Chinese with English abstract)[35]Junaedi A, Jung W S, Chung I M, Kim K H. Differentially expressed genes of potentially allelopathic rice in response against barnyardgrass. J Crop Sci Biotechnol, 2007, 10: 231–236[36]Pazares J, Ghosal D, Wienand U, Peterson P A, Saedler H. There gulatory C1 locus of Zea mays encodes a proterin with homology to myb-oncogene products and with structural similarities to transcriptional activators. EMBO J, 1987, 6: 3553–3558[37]Cone K C, Burr F A, Burr B. Molecular analysis of the maize anthocyanin regulatory locus C 1. Proc Natl Acad Sci USA, 1986, 83: 9631–9635[38]Moyano E, Martinez-Garcia J F, Martin C. Apparent redundancy in myb gene function provides gearing for the control of flavonoid biosynthesis in Antirrhinum flowers. Plant Cell, 1996, 8: 1519–1532[39]Legay S, Lacombe E, Goicoechea M, Brière C, Séguin A, Mackay J, Grima-Pettenati J. Molecular characterization of EgMYB1, a putative transcriptional repressor of the lignin biosynthetic pathway. Plant Sci, 2007, 173: 542–549 |
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