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作物学报 ›› 2015, Vol. 41 ›› Issue (04): 531-538.doi: 10.3724/SP.J.1006.2015.00531

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

OsMYB调控化感水稻酚酸类物质合成及其抑草作用

沈荔花1,2,李碧凉1,任勇杰1,李程勋1,钟永嘉2,方长旬1,2,林文雄1,2,*   

  1. 1福建农林大学生命科学学院,福建福州 350002;2福建农林大学农业生态研究所,福建福州 350002
  • 收稿日期:2014-11-18 修回日期:2015-02-06 出版日期:2015-04-12 网络出版日期:2015-03-03
  • 基金资助:

    本研究由国家自然科学基金项目(31271670, 31300336), 教育部高等学校博士学科点专项科研基金优先发展领域(20133515130001)和福建省自然科学基金项目(2010J05045,2012J01075)资助。

Phenolic Acid Synthesis of Allelopathic Rice Regulated by OsMYB and Its Weed Inhibition

SHEN Li-Hua1,2,LI Bi-Liang1,REN Yong-Jie1,LI Cheng-Xun1,ZHONG Yong-Jia2,FANG Chang-Xun1,2,LIN Wen-Xiong1,2,*   

  1. 1 College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Agro-ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2014-11-18 Revised:2015-02-06 Published:2015-04-12 Published online:2015-03-03

摘要:

基因表达调控是水稻化感作用形成的重要基础。本研究对化感水稻PI312777 (Oryza sativa L.)的OsMYB (CT829537)基因分别进行过表达(overexpression, OE)和RNA干扰(RNAi),再与稗草(Echinochloa crusgalli, BYG)共培养,以野生型PI312777为对照。结果发现,与稗草共培养下CT829537-OEPI312777的酚类代谢关键酶基因表达上调,根系及其水培液中的总酚酸浓度增加,抑草能力增强;相同处理下CT829537-RNAiPI312777则相反,其酚类代谢关键酶基因较野生型植株表达下调,总酚酸浓度降低,抑草能力下降。这表明OsMYB (CT829537)通过调节化感水稻的酚酸类物质合成进而影响其抑草能力。

关键词: 水稻, 化感作用, 转录因子, 酚酸, 基因表达调控

Abstract:

Regulation of gene expression is a vital process in the formation of rice allelopathy. In this study, allelopathic rice PI312777 (Oryza sativa L.) was modified by RNA interference (RNAi) and overexpression(OE) technologies to inhibit or enhance gene expression of OsMYB (CT829537) in PI312777 respectively. The results showed that up-regulation of phenolic synthesis related genes was found in CT829537-OE transgenic PI312777 co-cultured with barnyardgrass (BYG), compared with that in wild type. However, the reverse was true in the CT829537-RNAi transgenic PI312777. Up-regulation of the gene expression in CT829537-OE transgenic PI312777 increased phenolic acids contents in rice root and root exudates, which led to enhance allelopathic inhibition on barnyardgrass. In contrast, decreases of phenolic acids contents and weed inhibition were found in CT829537-RNAi transgenic PI312777. These results implied that CT829537 is responsible for regulating phenolic synthesis in allelopathic rice PI312777 and then enhances allelopathic inhibition on weeds.

Key words: Rice, Allelopathy, Transcription factor, Phenolic acid, Gene expression regulation

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