欢迎访问作物学报,今天是

作物学报 ›› 2015, Vol. 41 ›› Issue (07): 1121-1126.doi: 10.3724/SP.J.1006.2015.01121

• 研究简报 • 上一篇    下一篇

甘蓝型油菜株高性状的全基因组关联分析

唐敏强1,程晓晖1,童超波1,2,刘越英1,赵传纪1,董彩华1,2,于景印1,马小艮1,黄军艳1,2,*,刘胜毅1,2   

  1. 1中国农业科学院油料作物研究所 / 农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062;2湖北大学 / 物资源绿色转化湖北省协同创新中心,湖北武汉430062
  • 收稿日期:2015-01-12 修回日期:2015-05-04 出版日期:2015-07-12 网络出版日期:2015-05-15
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2013AA102602), 国家重点基础研究发展计划(973计划)项目(2011CB109305), 国家自然科学基金项目(31301039)和湖北农业科技创新中心项目资助。

Genome-wide Association Analysis of Plant Height in Rapeseed (Brassica napus)

TANG Min-Qiang1,CHENG Xiao-Hui1,TONG Chao-Bo1,2,LIU Yue-Ying1,ZHAO Chuan-Ji1,DONG Cai-Hua1,2,YU Jing-Yin1,MA Xiao-Gen1,HUANG Jun-Yan1,2,*,LIU Sheng-Yi1,2   

  1. 1 Oil Crops Research Institute, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China?; 2 Hubei university / Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources ,Wuhan 430062, China
  • Received:2015-01-12 Revised:2015-05-04 Published:2015-07-12 Published online:2015-05-15

摘要:

适当的株高不仅是抗倒性和机械化收获的需要,而且是建立高产理想株型的需要。本研究测定来自世界油菜主产国的371份种质资源材料构成的自然群体的株高,利用60K SNP芯片对该群体进行基因型检测,获得了21 856SNP标记,利用这些标记和株高数据进行全基因组关联分析结果表明:(1)检测到4个与株高显著关联的SNP,即Bn-A07-p3583161Bn-scaff_22790_1-p1271170Bn-scaff_20735_1-p42779Bn-scaff_18702_1-p589589,分别分布于A07C01C02染色体;它们对表型变异的解释率分别为11.33%11.75%12.31%10.97%(2)参考基因组信息,在上述前3SNP附近的4929.569.5 kb处分别找到一个与株高性状相关的候选基因;(3) 4个显著标记SNP各自的等位基因间表型均值差异分别为11.0915.1210.488.93 cm,双尾t测验结果表明各等位基因组间差异显著。

关键词: 甘蓝型油菜, 基因组关联分析, SNP, 株高, 候选基因

Abstract:

Appropriate plant height is a trait related to lodging resistance and mechanical harvest, and ideal plant height is a basis of ideotype for high yield. The plant height of 371 rapeseed accessions collected from different growing regions in the world was measured in field experiments, all accessions were genotyped using a 60K SNP chip and 21856 informative SNPs were acquired, then the data of SNPs and plant height were used for genome-wide association analysis. Major results were as follows: 1) 4 significant SNPs related to plant height were detected, including Bn-A07-p3583161, Bn-scaff_22790_1-p1271170, Bn-scaff_20735_1-p42779, and Bn-scaff_18702_1-p589589, which located in A07, C01 and C02 chromosomes, and explained 11.33%, 11.75%, 12.31%, and 10.97% of phenotypic variation, respectively; 2) According to the reference genome annotation information, three candidate genes corresponding to the first three significant SNP were predicted, each gene was locates in 492.0, 9.5, and 69.5 kb away from the three SNP, respectively ; 3) The significant (two-tailed t-test) difference of plant height between means of two allelic groups of accessions corresponding to each significant SNP was 11.09, 15.12, 10.48, and 8.93 cm, respectively.

Key words: Brassica napus L., Genome-wide association analysis, SNP, Plant height, Candidate gene

[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[3] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[4] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[5] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[6] 孙思敏, 韩贝, 陈林, 孙伟男, 张献龙, 杨细燕. 棉花苗期根系分型及根系性状的关联分析[J]. 作物学报, 2022, 48(5): 1081-1090.
[7] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[8] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[9] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[10] 刘丹, 周彩娥, 王晓婷, 吴启蒙, 张旭, 王琪琳, 曾庆东, 康振生, 韩德俊, 吴建辉. 利用集群分离分析结合高密度芯片快速定位小麦成株期抗条锈病基因YrC271[J]. 作物学报, 2022, 48(3): 553-564.
[11] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589.
[12] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[13] 王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[14] 渠建洲, 冯文豪, 张兴华, 徐淑兔, 薛吉全. 基于全基因组关联分析解析玉米籽粒大小的遗传结构[J]. 作物学报, 2022, 48(2): 304-319.
[15] 郑向华, 叶俊华, 程朝平, 魏兴华, 叶新福, 杨窑龙. 利用SNP标记进行水稻品种籼粳鉴定[J]. 作物学报, 2022, 48(2): 342-352.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!