关键词: 甘蓝型油菜, 基因组关联分析, SNP, 株高, 候选基因

Abstract:

Appropriate plant height is a trait related to lodging resistance and mechanical harvest, and ideal plant height is a basis of ideotype for high yield. The plant height of 371 rapeseed accessions collected from different growing regions in the world was measured in field experiments, all accessions were genotyped using a 60K SNP chip and 21856 informative SNPs were acquired, then the data of SNPs and plant height were used for genome-wide association analysis. Major results were as follows: 1) 4 significant SNPs related to plant height were detected, including Bn-A07-p3583161, Bn-scaff_22790_1-p1271170, Bn-scaff_20735_1-p42779, and Bn-scaff_18702_1-p589589, which located in A07, C01 and C02 chromosomes, and explained 11.33%, 11.75%, 12.31%, and 10.97% of phenotypic variation, respectively; 2) According to the reference genome annotation information, three candidate genes corresponding to the first three significant SNP were predicted, each gene was locates in 492.0, 9.5, and 69.5 kb away from the three SNP, respectively ; 3) The significant (two-tailed t-test) difference of plant height between means of two allelic groups of accessions corresponding to each significant SNP was 11.09, 15.12, 10.48, and 8.93 cm, respectively.

Key words: Brassica napus L., Genome-wide association analysis, SNP, Plant height, Candidate gene