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作物学报 ›› 2016, Vol. 42 ›› Issue (01): 51-57.doi: 10.3724/SP.J.1006.2016.00051

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

DTA-6对大豆花荚脱落纤维素酶和GmAC基因表达的调控

崔洪秋1,2**,冯乃杰1**,孙福东1,刘涛1,李建英2,杜吉到1,韩毅强1,郑殿峰1,*   

  1. 1黑龙江八一农垦大学农学院,黑龙江大庆 163319;2黑龙江省农业科学院大庆分院,黑龙江大庆 163316
  • 收稿日期:2015-02-28 修回日期:2015-09-06 出版日期:2016-01-12 网络出版日期:2015-10-08
  • 通讯作者: 郑殿峰, E-mail: dianfeng69@gmail.com
  • 基金资助:

    本研究由国家自然科学基金项目(31171503, 31271652), 国家科技支撑计划项目(2012BAD20B04), 黑龙江省研究生创新科研项目(YJSCX2012-253HLJ), 黑龙江省杰出青年基金项目(JC201309)和黑龙江农垦总局科技攻关项目(HNK12A-06-03, HNK12A-09-02)资助。

Regulation of DTA-6 by Abscission Cellulase and GmAC Gene Expression in Flowers and Pods of Soybean

CUI Hong-Qiu1,2,**,FENG Nai-Jie1,**,SUN Fu-Dong1,LIU Tao1,LI Jian-Ying2,DU Ji-Dao1,HAN Yi-Qiang1,ZHEN Dian-Feng1,*   

  1. 1Agronomy of College / Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2 Daqing branch College / Heilongjiang Academy of Agricultural Science, Daqing163316, China
  • Received:2015-02-28 Revised:2015-09-06 Published:2016-01-12 Published online:2015-10-08
  • Contact: 郑殿峰, E-mail: dianfeng69@gmail.com
  • Supported by:

    This research was supported by the National Natural Science Foundation of China (31171503, 31271652), Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (2012BAD20B04), Heilongjiang Provincial Graduate Science and Technology Innovation Projects (YJSCX2012-253HLJ), Heilongjiang Province Funds for Distinguished Young Scientists (JC201309), and Key Scientific and Technological Projects of Heilongjiang Agriculture and Reclamation Bureau (HNK12A-06-03, HNK12A-09-02).

摘要:

叶面喷施生长调节剂(PGRs)可以调控大豆花荚脱落。2012—2013年在大庆林甸县黑龙江八一农垦大学试验基地采用大田小区试验,R1期对大豆绥农28(SN28)、垦丰16 (KF16)和合丰50 (HF50)分别叶面喷施DTA-6调节剂,对调控花荚离区脱落纤维素酶(GmAC)基因相对表达量、荚脱落纤维素酶(AC)活性、花荚脱落率和产量进行了研究。结果表明:DTA-6抑制大豆花荚离区GmAC基因相对表达量,最大可达51% (±CK%);大豆荚的AC活性能够在喷药后阶段性地被降低,在不同品种上增加和降低存在差异;能够显著降低花荚脱落率(P<0.05);能够显著增加产量(P<0.05)。植物生长调节剂DTA-6是通过降低花荚离区GmAC基因相对表达量,调节AC活性,从而减少植株的花荚脱落率,以实现对产量的调控。

关键词: DTA-6, GmAC基因表达, AC, 花荚脱落率, 产量

Abstract:

It is effective to regulate abscission of soybean flowers and pods by spraying plant growth regulators (PGRs). This study was carried out to determine the effect of DTA-6 on abscission cellulose (AC) activity, abscission cellulose (GmAC) gene expressive, abscission of flowers and pods and yield in soybean. DTA-6 was foliage sprayed at R1 stage on three varieties of Suinong 28 (SN28), Kenfeng 16 (KF16), and Hefeng 50 (HF50) in 2012 and 2013. Our results demonstrated that DTA-6 treatment inhibited GmAC gene expression in abscission zone of flower and pod, with the maximum reduction of 51% among three varieties compared with control. The abscission cellulase activity was periodically decreased by DTA-6, with different decrements among soybean cultivars. DTA-6 significantly decreased (P<0.05) abscission rate of soybean flowers and pods. And significantly increased yield (P<0.05). The relative expression of abscission cellulase gene (GmAC) in abscission zone of flowers-pods was decreased and AC activity was regulated by DTA-6, resulting in reduced abscission rate of soybean flowers and pods and promoted yield.

Key words: DTA-6, GmAC gene expressive, Abscission cellulase, Flowers and pods abscission, Yield

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