欢迎访问作物学报，今天是
作物学报 ›› 2023, Vol. 49 ›› Issue (11): 2991-3006.doi: 10.3724/SP.J.1006.2023.34027
陈吴钧(
), 刘江栋, 蒋凯旋, 王幼平, 蒋金金()
CHEN Wu-Jun(), LIU Jiang-Dong, JIANG Kai-Xuan, WANG You-Ping, JIANG Jin-Jin()
摘要：
植物特异的KNOTTED-LIKE HOMEOBOX (KNOX)蛋白属于转录调控因子家族, 该家族在植物生长发育过程和各种胁迫应答中发挥着重要的作用。KNOX蛋白中存在4个保守的结构域: TALE (three amino acid loop extension)类型的HD (homeodomain)、ELK结构域, 以及2个亚结构域KNOX1和KNOX2。目前, 甘蓝型油菜的BnKNOX基因家族还未有系统的研究报道。本研究通过生物信息学分析鉴定获得甘蓝型油菜的36个BnKNOX家族成员。通过序列比对和系统发育树分析, 将其分为3个亚家族(I、II和M类)。进化分析表明, 全基因组复制(whole genome duplication, WGD)和片段复制(segmental duplication)是BnKNOX基因家族扩张的主要动力。基于甘蓝型油菜不同发育时期组织/器官的RNA-seq分析发现, 该基因家族的BnKNAT25/26/29/30在胚乳和种子发育过程中特异表达, 而BnKNAT31/32/34在成熟种子中高表达。通过BnKNOX家族的顺式作用元件分析和非生物胁迫条件下的表达模式分析, 本文鉴定获得17个响应干旱和渗透胁迫的BnKNOX成员。
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